Moderately enhancing cytokinin level by down-regulation of GhCKX expression in cotton concurrently increases fiber and seed yield
Cotton is the leading natural fiber crop in the world. Cotton seeds are also an important oil and protein source. However, enhancement of fiber abundance usually leads to a smaller seed. Thus, it has become a challenge for cotton breeding to concurrently increase fiber yield and seed yield. To impro...
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| Veröffentlicht in: | Molecular breeding 2015-02, Vol.35 (2), p.60-11, Article 60 |
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| container_title | Molecular breeding |
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| creator | Zhao, Juan Bai, Wenqin Zeng, Qiwei Song, Shuiqing Zhang, Mi Li, Xianbi Hou, Lei Xiao, Yuehua Luo, Ming Li, Demou Luo, Xiaoying Pei, Yan |
| description | Cotton is the leading natural fiber crop in the world. Cotton seeds are also an important oil and protein source. However, enhancement of fiber abundance usually leads to a smaller seed. Thus, it has become a challenge for cotton breeding to concurrently increase fiber yield and seed yield. To improve cotton yield, we elevated the endogenous cytokinin level in transgenic cotton by constitutive suppression of cytokinin dehydrogenase (CKX), a key negative regulator controlling endogenous cytokinin in plants. The slightly and moderately suppressed transgenic cotton plants showed normal growth and development, while the severely suppressed plants exhibited a typical cytokinin-overproduction alteration. The suppression of CKX led to an enhancement of endogenous cytokinins in transgenic cotton plants. Total cytokinins in moderately suppressed lines, CR-3 and CR-6, increased by 20.4 and 55.5 % respectively, and that in the severely suppressed line (CR-13) increased by 134.2 % compared to the wild type. The moderately suppressed lines showed a delay in leaf senescence, higher photosynthesis, more fruiting branches and bolls, and bigger seed size. Field trials showed that seed yield and lint yield of the moderately suppressed CR-6 line increased by 15.4 and 20.0 %, respectively. Meanwhile, the enhanced cytokinin level in transgenic cottons did not show significant influence on fiber qualities. Our data demonstrated that CKX is a promising gene for crop yield improvement. |
| doi_str_mv | 10.1007/s11032-015-0232-6 |
| format | Article |
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Cotton seeds are also an important oil and protein source. However, enhancement of fiber abundance usually leads to a smaller seed. Thus, it has become a challenge for cotton breeding to concurrently increase fiber yield and seed yield. To improve cotton yield, we elevated the endogenous cytokinin level in transgenic cotton by constitutive suppression of cytokinin dehydrogenase (CKX), a key negative regulator controlling endogenous cytokinin in plants. The slightly and moderately suppressed transgenic cotton plants showed normal growth and development, while the severely suppressed plants exhibited a typical cytokinin-overproduction alteration. The suppression of CKX led to an enhancement of endogenous cytokinins in transgenic cotton plants. Total cytokinins in moderately suppressed lines, CR-3 and CR-6, increased by 20.4 and 55.5 % respectively, and that in the severely suppressed line (CR-13) increased by 134.2 % compared to the wild type. The moderately suppressed lines showed a delay in leaf senescence, higher photosynthesis, more fruiting branches and bolls, and bigger seed size. Field trials showed that seed yield and lint yield of the moderately suppressed CR-6 line increased by 15.4 and 20.0 %, respectively. Meanwhile, the enhanced cytokinin level in transgenic cottons did not show significant influence on fiber qualities. Our data demonstrated that CKX is a promising gene for crop yield improvement.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-015-0232-6</identifier><identifier>PMID: 25653571</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Agricultural production ; Biomedical and Life Sciences ; Biotechnology ; bolls ; branches ; Cotton ; cottonseed ; Crop yield ; Cytokinins ; fiber quality ; field experimentation ; fruiting ; Fruits ; gene expression regulation ; genes ; Gossypium hirsutum ; growth and development ; leaves ; Life Sciences ; Lint ; lint yield ; Molecular biology ; oils ; Photosynthesis ; Plant biology ; Plant breeding ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Protein sources ; seed yield ; Seeds ; Senescence ; Transgenic plants</subject><ispartof>Molecular breeding, 2015-02, Vol.35 (2), p.60-11, Article 60</ispartof><rights>The Author(s) 2015</rights><rights>Molecular Breeding is a copyright of Springer, (2015). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-276a9fda4f70f51202a8f915a01886ee74c5bb7a345bde71fb217cd6a22640b23</citedby><cites>FETCH-LOGICAL-c560t-276a9fda4f70f51202a8f915a01886ee74c5bb7a345bde71fb217cd6a22640b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-015-0232-6$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-015-0232-6$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,778,782,883,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25653571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Juan</creatorcontrib><creatorcontrib>Bai, Wenqin</creatorcontrib><creatorcontrib>Zeng, Qiwei</creatorcontrib><creatorcontrib>Song, Shuiqing</creatorcontrib><creatorcontrib>Zhang, Mi</creatorcontrib><creatorcontrib>Li, Xianbi</creatorcontrib><creatorcontrib>Hou, Lei</creatorcontrib><creatorcontrib>Xiao, Yuehua</creatorcontrib><creatorcontrib>Luo, Ming</creatorcontrib><creatorcontrib>Li, Demou</creatorcontrib><creatorcontrib>Luo, Xiaoying</creatorcontrib><creatorcontrib>Pei, Yan</creatorcontrib><title>Moderately enhancing cytokinin level by down-regulation of GhCKX expression in cotton concurrently increases fiber and seed yield</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><addtitle>Mol Breed</addtitle><description>Cotton is the leading natural fiber crop in the world. Cotton seeds are also an important oil and protein source. However, enhancement of fiber abundance usually leads to a smaller seed. Thus, it has become a challenge for cotton breeding to concurrently increase fiber yield and seed yield. To improve cotton yield, we elevated the endogenous cytokinin level in transgenic cotton by constitutive suppression of cytokinin dehydrogenase (CKX), a key negative regulator controlling endogenous cytokinin in plants. The slightly and moderately suppressed transgenic cotton plants showed normal growth and development, while the severely suppressed plants exhibited a typical cytokinin-overproduction alteration. The suppression of CKX led to an enhancement of endogenous cytokinins in transgenic cotton plants. Total cytokinins in moderately suppressed lines, CR-3 and CR-6, increased by 20.4 and 55.5 % respectively, and that in the severely suppressed line (CR-13) increased by 134.2 % compared to the wild type. The moderately suppressed lines showed a delay in leaf senescence, higher photosynthesis, more fruiting branches and bolls, and bigger seed size. Field trials showed that seed yield and lint yield of the moderately suppressed CR-6 line increased by 15.4 and 20.0 %, respectively. Meanwhile, the enhanced cytokinin level in transgenic cottons did not show significant influence on fiber qualities. Our data demonstrated that CKX is a promising gene for crop yield improvement.</description><subject>Agricultural production</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>bolls</subject><subject>branches</subject><subject>Cotton</subject><subject>cottonseed</subject><subject>Crop yield</subject><subject>Cytokinins</subject><subject>fiber quality</subject><subject>field experimentation</subject><subject>fruiting</subject><subject>Fruits</subject><subject>gene expression regulation</subject><subject>genes</subject><subject>Gossypium hirsutum</subject><subject>growth and development</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>Lint</subject><subject>lint yield</subject><subject>Molecular biology</subject><subject>oils</subject><subject>Photosynthesis</subject><subject>Plant biology</subject><subject>Plant breeding</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Protein sources</subject><subject>seed yield</subject><subject>Seeds</subject><subject>Senescence</subject><subject>Transgenic plants</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkstu1TAQhiMEoqXwAGzAEhs2AV_iSzZI6AgKoogFVGJnOc74HJcc-2AnhSx5cxyllMsCWHnk-eaf8fivqvsEPyEYy6eZEMxojQmvMS2BuFEdEy5p3UqlbpaYKVwz2bCj6k7OF7jUtELcro4oF5xxSY6rb29jD8mMMMwIws4E68MW2XmMn3zwAQ1wCQPqZtTHL6FOsJ0GM_oYUHTodLd58xHB10OCnJe7wts4jnE5gp1SgjAWXR9sApMhI-c7SMiEHmWAHs0ehv5udcuZIcO9q_OkOn_54sPmVX327vT15vlZbbnAY02lMK3rTeMkdpxQTI1yLeEGE6UEgGws7zppWMO7HiRxHSXS9sJQKhrcUXZSPVt1D1O3h96W2ZIZ9CH5vUmzjsbr3zPB7_Q2XuqG4VYpVgQeXwmk-HmCPOq9zxaGwQSIU9ZEUSEIb1v-b1RIigUpv_QfqMANVoos6KM_0Is4pVCWpiltGZZlgKU3WSmbYs4J3PUTCdaLbfRqG11soxfbaFFqHvy6m-uKHz4pAF2BXFJhC-ln67-pPlyLnInabJPP-vw9LUAxYiOIkuw7KkbYKA</recordid><startdate>20150201</startdate><enddate>20150201</enddate><creator>Zhao, Juan</creator><creator>Bai, Wenqin</creator><creator>Zeng, Qiwei</creator><creator>Song, Shuiqing</creator><creator>Zhang, Mi</creator><creator>Li, Xianbi</creator><creator>Hou, Lei</creator><creator>Xiao, Yuehua</creator><creator>Luo, Ming</creator><creator>Li, Demou</creator><creator>Luo, Xiaoying</creator><creator>Pei, Yan</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150201</creationdate><title>Moderately enhancing cytokinin level by down-regulation of GhCKX expression in cotton concurrently increases fiber and seed yield</title><author>Zhao, Juan ; Bai, Wenqin ; Zeng, Qiwei ; Song, Shuiqing ; Zhang, Mi ; Li, Xianbi ; Hou, Lei ; Xiao, Yuehua ; Luo, Ming ; Li, Demou ; Luo, Xiaoying ; Pei, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-276a9fda4f70f51202a8f915a01886ee74c5bb7a345bde71fb217cd6a22640b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Agricultural production</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>bolls</topic><topic>branches</topic><topic>Cotton</topic><topic>cottonseed</topic><topic>Crop yield</topic><topic>Cytokinins</topic><topic>fiber quality</topic><topic>field experimentation</topic><topic>fruiting</topic><topic>Fruits</topic><topic>gene expression regulation</topic><topic>genes</topic><topic>Gossypium hirsutum</topic><topic>growth and development</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>Lint</topic><topic>lint yield</topic><topic>Molecular biology</topic><topic>oils</topic><topic>Photosynthesis</topic><topic>Plant biology</topic><topic>Plant breeding</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Protein sources</topic><topic>seed yield</topic><topic>Seeds</topic><topic>Senescence</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Juan</creatorcontrib><creatorcontrib>Bai, Wenqin</creatorcontrib><creatorcontrib>Zeng, Qiwei</creatorcontrib><creatorcontrib>Song, Shuiqing</creatorcontrib><creatorcontrib>Zhang, Mi</creatorcontrib><creatorcontrib>Li, Xianbi</creatorcontrib><creatorcontrib>Hou, Lei</creatorcontrib><creatorcontrib>Xiao, Yuehua</creatorcontrib><creatorcontrib>Luo, Ming</creatorcontrib><creatorcontrib>Li, Demou</creatorcontrib><creatorcontrib>Luo, Xiaoying</creatorcontrib><creatorcontrib>Pei, Yan</creatorcontrib><collection>AGRIS</collection><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Juan</au><au>Bai, Wenqin</au><au>Zeng, Qiwei</au><au>Song, Shuiqing</au><au>Zhang, Mi</au><au>Li, Xianbi</au><au>Hou, Lei</au><au>Xiao, Yuehua</au><au>Luo, Ming</au><au>Li, Demou</au><au>Luo, Xiaoying</au><au>Pei, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Moderately enhancing cytokinin level by down-regulation of GhCKX expression in cotton concurrently increases fiber and seed yield</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><addtitle>Mol Breed</addtitle><date>2015-02-01</date><risdate>2015</risdate><volume>35</volume><issue>2</issue><spage>60</spage><epage>11</epage><pages>60-11</pages><artnum>60</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Cotton is the leading natural fiber crop in the world. Cotton seeds are also an important oil and protein source. However, enhancement of fiber abundance usually leads to a smaller seed. Thus, it has become a challenge for cotton breeding to concurrently increase fiber yield and seed yield. To improve cotton yield, we elevated the endogenous cytokinin level in transgenic cotton by constitutive suppression of cytokinin dehydrogenase (CKX), a key negative regulator controlling endogenous cytokinin in plants. The slightly and moderately suppressed transgenic cotton plants showed normal growth and development, while the severely suppressed plants exhibited a typical cytokinin-overproduction alteration. The suppression of CKX led to an enhancement of endogenous cytokinins in transgenic cotton plants. Total cytokinins in moderately suppressed lines, CR-3 and CR-6, increased by 20.4 and 55.5 % respectively, and that in the severely suppressed line (CR-13) increased by 134.2 % compared to the wild type. The moderately suppressed lines showed a delay in leaf senescence, higher photosynthesis, more fruiting branches and bolls, and bigger seed size. Field trials showed that seed yield and lint yield of the moderately suppressed CR-6 line increased by 15.4 and 20.0 %, respectively. Meanwhile, the enhanced cytokinin level in transgenic cottons did not show significant influence on fiber qualities. Our data demonstrated that CKX is a promising gene for crop yield improvement.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>25653571</pmid><doi>10.1007/s11032-015-0232-6</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agricultural production Biomedical and Life Sciences Biotechnology bolls branches Cotton cottonseed Crop yield Cytokinins fiber quality field experimentation fruiting Fruits gene expression regulation genes Gossypium hirsutum growth and development leaves Life Sciences Lint lint yield Molecular biology oils Photosynthesis Plant biology Plant breeding Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Protein sources seed yield Seeds Senescence Transgenic plants |
| title | Moderately enhancing cytokinin level by down-regulation of GhCKX expression in cotton concurrently increases fiber and seed yield |
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