Relationship between Interferon Production and Interferon Messenger RNA Synthesis in Human Fibroblasts
Poly(A) containing mRNA prepared from poly(rI)· poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synth...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1977-04, Vol.74 (4), p.1483-1487 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | N. Babu Kishan Raj Pitha, Paula M. |
| description | Poly(A) containing mRNA prepared from poly(rI)· poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synthesized in vitro fulfilled the criteria for human interferon-namely, its antiviral activity was species specific, and its activity was completely neutralized by antiserum to human fibroblast interferon. The amount of interferon synthesized in human fibroblasts induced by poly(rI)· poly(rC) (normal induction) and poly(rI)· poly(rC) in the presence of cycloheximide (superinduction) was compared to the amount of translatable interferon mRNA both in the wheat germ cell-free system and the Xenopus oöcyte system. Although the production of interferon after the termination of transcription by actinomycin D was markedly increased in superinduced cells, the measurable amount of interferon mRNA as assayed in the oöcyte system was only slightly higher in superinduced cells than in cells induced with poly(rI)· poly(rC) alone. When compared in the wheat germ cell-free system, however, the translational product of mRNA preparation from cells induced with poly(rI)· poly(rC) alone was inactive while that from superinduced cells was active. |
| doi_str_mv | 10.1073/pnas.74.4.1483 |
| format | Article |
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Babu Kishan Raj ; Pitha, Paula M.</creator><creatorcontrib>N. Babu Kishan Raj ; Pitha, Paula M.</creatorcontrib><description>Poly(A) containing mRNA prepared from poly(rI)· poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synthesized in vitro fulfilled the criteria for human interferon-namely, its antiviral activity was species specific, and its activity was completely neutralized by antiserum to human fibroblast interferon. The amount of interferon synthesized in human fibroblasts induced by poly(rI)· poly(rC) (normal induction) and poly(rI)· poly(rC) in the presence of cycloheximide (superinduction) was compared to the amount of translatable interferon mRNA both in the wheat germ cell-free system and the Xenopus oöcyte system. Although the production of interferon after the termination of transcription by actinomycin D was markedly increased in superinduced cells, the measurable amount of interferon mRNA as assayed in the oöcyte system was only slightly higher in superinduced cells than in cells induced with poly(rI)· poly(rC) alone. When compared in the wheat germ cell-free system, however, the translational product of mRNA preparation from cells induced with poly(rI)· poly(rC) alone was inactive while that from superinduced cells was active.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.74.4.1483</identifier><identifier>PMID: 266188</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antiserum ; Antivirals ; Cell free system ; Cells, Cultured ; Cycloheximide - pharmacology ; Female ; Fibroblasts ; Fibroblasts - metabolism ; Germ cells ; Humans ; Interferons ; Interferons - biosynthesis ; Kinetics ; Leukocytes - metabolism ; Male ; Messenger RNA ; Oocytes - metabolism ; Plants - metabolism ; Poly I-C ; Protein Biosynthesis - drug effects ; RNA ; RNA, Messenger - biosynthesis ; Spermine - pharmacology ; Transcription, Genetic ; Triticum - metabolism ; Wheat ; Xenopus</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1977-04, Vol.74 (4), p.1483-1487</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-bfc8e9ac56edadf99c5cd5ddc6e15bcc321cdf9527bfd692457d247d4cf8f9d63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/74/4.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/66727$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/66727$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/266188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>N. Babu Kishan Raj</creatorcontrib><creatorcontrib>Pitha, Paula M.</creatorcontrib><title>Relationship between Interferon Production and Interferon Messenger RNA Synthesis in Human Fibroblasts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Poly(A) containing mRNA prepared from poly(rI)· poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synthesized in vitro fulfilled the criteria for human interferon-namely, its antiviral activity was species specific, and its activity was completely neutralized by antiserum to human fibroblast interferon. The amount of interferon synthesized in human fibroblasts induced by poly(rI)· poly(rC) (normal induction) and poly(rI)· poly(rC) in the presence of cycloheximide (superinduction) was compared to the amount of translatable interferon mRNA both in the wheat germ cell-free system and the Xenopus oöcyte system. Although the production of interferon after the termination of transcription by actinomycin D was markedly increased in superinduced cells, the measurable amount of interferon mRNA as assayed in the oöcyte system was only slightly higher in superinduced cells than in cells induced with poly(rI)· poly(rC) alone. When compared in the wheat germ cell-free system, however, the translational product of mRNA preparation from cells induced with poly(rI)· poly(rC) alone was inactive while that from superinduced cells was active.</description><subject>Animals</subject><subject>Antiserum</subject><subject>Antivirals</subject><subject>Cell free system</subject><subject>Cells, Cultured</subject><subject>Cycloheximide - pharmacology</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Fibroblasts - metabolism</subject><subject>Germ cells</subject><subject>Humans</subject><subject>Interferons</subject><subject>Interferons - biosynthesis</subject><subject>Kinetics</subject><subject>Leukocytes - metabolism</subject><subject>Male</subject><subject>Messenger RNA</subject><subject>Oocytes - metabolism</subject><subject>Plants - metabolism</subject><subject>Poly I-C</subject><subject>Protein Biosynthesis - drug effects</subject><subject>RNA</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Spermine - pharmacology</subject><subject>Transcription, Genetic</subject><subject>Triticum - metabolism</subject><subject>Wheat</subject><subject>Xenopus</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAURi3EayhsWSCQsuouwXb8iBcsqorSSuWhAmvLsa87qTL2YDtA_z2JZhgNG1ZX8jnfvZY-hF4S3BAs27fbYHIjWcMawrr2AVoRrEgtmMIP0QpjKuuOUfYUPcv5DmOseIefoMdUCNJ1K-RvYDRliCGvh23VQ_kFEKqrUCB5SDFUX1J0k12MygR3TD5CzhBuIVU3n86qr_ehrCEPuRpCdTltTKguhj7FfjS55OfokTdjhhf7eYK-X7z_dn5ZX3_-cHV-dl1bxnmpe287UMZyAc44r5Tl1nHnrADCe2tbSuz8zKnsvROKMi4dZdIx6zuvnGhP0Lvd3u3Ub8BZCCWZUW_TsDHpXkcz6H9JGNb6Nv7UrMUdVnP-dJ9P8ccEuejNkC2MowkQp6y7VmEh6HKo2Yk2xZwT-MMNgvXSi1560ZJpppde5sDr458d9F0RM36zx0vsLzyOn_6Paz-NY4HfZRZf7cS7XGI6mEJIKts_Jquukw</recordid><startdate>19770401</startdate><enddate>19770401</enddate><creator>N. Babu Kishan Raj</creator><creator>Pitha, Paula M.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19770401</creationdate><title>Relationship between Interferon Production and Interferon Messenger RNA Synthesis in Human Fibroblasts</title><author>N. Babu Kishan Raj ; Pitha, Paula M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-bfc8e9ac56edadf99c5cd5ddc6e15bcc321cdf9527bfd692457d247d4cf8f9d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Antiserum</topic><topic>Antivirals</topic><topic>Cell free system</topic><topic>Cells, Cultured</topic><topic>Cycloheximide - pharmacology</topic><topic>Female</topic><topic>Fibroblasts</topic><topic>Fibroblasts - metabolism</topic><topic>Germ cells</topic><topic>Humans</topic><topic>Interferons</topic><topic>Interferons - biosynthesis</topic><topic>Kinetics</topic><topic>Leukocytes - metabolism</topic><topic>Male</topic><topic>Messenger RNA</topic><topic>Oocytes - metabolism</topic><topic>Plants - metabolism</topic><topic>Poly I-C</topic><topic>Protein Biosynthesis - drug effects</topic><topic>RNA</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Spermine - pharmacology</topic><topic>Transcription, Genetic</topic><topic>Triticum - metabolism</topic><topic>Wheat</topic><topic>Xenopus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>N. Babu Kishan Raj</creatorcontrib><creatorcontrib>Pitha, Paula M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>N. Babu Kishan Raj</au><au>Pitha, Paula M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between Interferon Production and Interferon Messenger RNA Synthesis in Human Fibroblasts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1977-04-01</date><risdate>1977</risdate><volume>74</volume><issue>4</issue><spage>1483</spage><epage>1487</epage><pages>1483-1487</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Poly(A) containing mRNA prepared from poly(rI)· poly(rC)-induced human fibroblasts stimulated [14C]leucine incorporation into protein in wheat germ cell-free extracts. For the translation of interferon mRNA into a biologically active product, the presence of spermine was essential. The protein synthesized in vitro fulfilled the criteria for human interferon-namely, its antiviral activity was species specific, and its activity was completely neutralized by antiserum to human fibroblast interferon. The amount of interferon synthesized in human fibroblasts induced by poly(rI)· poly(rC) (normal induction) and poly(rI)· poly(rC) in the presence of cycloheximide (superinduction) was compared to the amount of translatable interferon mRNA both in the wheat germ cell-free system and the Xenopus oöcyte system. Although the production of interferon after the termination of transcription by actinomycin D was markedly increased in superinduced cells, the measurable amount of interferon mRNA as assayed in the oöcyte system was only slightly higher in superinduced cells than in cells induced with poly(rI)· poly(rC) alone. When compared in the wheat germ cell-free system, however, the translational product of mRNA preparation from cells induced with poly(rI)· poly(rC) alone was inactive while that from superinduced cells was active.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>266188</pmid><doi>10.1073/pnas.74.4.1483</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1977-04, Vol.74 (4), p.1483-1487 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antiserum Antivirals Cell free system Cells, Cultured Cycloheximide - pharmacology Female Fibroblasts Fibroblasts - metabolism Germ cells Humans Interferons Interferons - biosynthesis Kinetics Leukocytes - metabolism Male Messenger RNA Oocytes - metabolism Plants - metabolism Poly I-C Protein Biosynthesis - drug effects RNA RNA, Messenger - biosynthesis Spermine - pharmacology Transcription, Genetic Triticum - metabolism Wheat Xenopus |
| title | Relationship between Interferon Production and Interferon Messenger RNA Synthesis in Human Fibroblasts |
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