Ornithine decarboxylase gene is overexpressed in colorectal carcinoma
AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma technology.METHODS: Total RNA was extracted from human colorecta...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2005-04, Vol.11 (15), p.2244-2248 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | Hu, Hai-Yan Liu, Xian-Xi Jiang, Chun-Ying Lu, Yi Liu, Shi-Lian Bian, Ji-Feng Wang, Xiao-Ming Geng, Zhao Zhang, Yan Zhang, Bing |
| description | AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma technology.METHODS: Total RNA was extracted from human colorectal cancer tissues and their normal counterpart tissues. ODC mRNA levels were examined by RT-PCR.ODC genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immunohistochemical and Western blotting assay.RESULTS: A cell line, which could steadily secrete antiODC mAb, was selected through subcloning four times. Western blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P |
| doi_str_mv | 10.3748/wjg.v11.i15.2244 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4305806</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>15502125</cqvip_id><wanfj_id>wjg200515007</wanfj_id><sourcerecordid>wjg200515007</sourcerecordid><originalsourceid>FETCH-LOGICAL-c516t-111288d0a313b920ed47538a5c9eab3f6d6efe6e058804810fc4e95249c4239b3</originalsourceid><addsrcrecordid>eNpVkU1P3DAQhq2Kqmyhd05VhFBvWcZfiXOphBD9kJC4wNlynEnWS9Ze7OwC_x6vdtXCyZL9zOOZeQk5ozDntVCXz8thvqV07qicMybEJzJjjDYlUwKOyIwC1GXDWX1Mvqa0BGCcS_aFHFOpqKo5n5Gbu-jdtHAeiw6tiW14eR1NwmLAfOVSEbYY8WUdMSXsCucLG8YQ0U5mLDJvnQ8rc0o-92ZM-O1wnpCHXzf313_K27vff6-vbksraTWVlFKmVAeGU942DLATteTKSNugaXlfdRX2WCFIpUAoCr0V2EgmGisYb1p-Qn7uvetNu8LOop-iGfU6upWJrzoYpz--eLfQQ9hqwbMTqiy42Aueje-NH_QybKLPLeu8SgYgqcw7y9iPwz8xPG0wTXrlksVxNB7DJumqrhnUFc8g7EEbQ0oR-3-9UNC7iHZenSPSOSK9iyiXfH8_w_-CQyYZOD84F8EPTy532Rr72LsRMySBUSb5GwVAmew</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67720763</pqid></control><display><type>article</type><title>Ornithine decarboxylase gene is overexpressed in colorectal carcinoma</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Hu, Hai-Yan ; Liu, Xian-Xi ; Jiang, Chun-Ying ; Lu, Yi ; Liu, Shi-Lian ; Bian, Ji-Feng ; Wang, Xiao-Ming ; Geng, Zhao ; Zhang, Yan ; Zhang, Bing</creator><creatorcontrib>Hu, Hai-Yan ; Liu, Xian-Xi ; Jiang, Chun-Ying ; Lu, Yi ; Liu, Shi-Lian ; Bian, Ji-Feng ; Wang, Xiao-Ming ; Geng, Zhao ; Zhang, Yan ; Zhang, Bing</creatorcontrib><description>AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma technology.METHODS: Total RNA was extracted from human colorectal cancer tissues and their normal counterpart tissues. ODC mRNA levels were examined by RT-PCR.ODC genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immunohistochemical and Western blotting assay.RESULTS: A cell line, which could steadily secrete antiODC mAb, was selected through subcloning four times. Western blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P<0.01). Immunohistochemical staining showed that colorectal carcinoma cells expressed a significantly higher level of ODC than normal colorectal mucosa (98.6±1.03% vs 5.26±5%, P<0.01).CONCLUSION: ODC gene overexpression is significantly related to human colorectal carcinoma. ODC gene expression may be a marker for the gene diagnosis and therapy of colorectal carcinoma.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v11.i15.2244</identifier><identifier>PMID: 15818733</identifier><language>eng</language><publisher>United States: Experimental Center of Medical Molecular Biology, Biochemistry and Molecular Biology Institute, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China%Department of Colo-proctology, The Affiliated Hospital of Shandong University of Tradition Chinese Medicine,Jinan 250012, Shandong Province, China</publisher><subject>Animals ; Antibodies, Monoclonal ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Colorectal Cancer ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - physiopathology ; Female ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Neoplastic ; Humans ; Mice ; Mice, Inbred BALB C ; Ornithine Decarboxylase - genetics ; Ornithine Decarboxylase - immunology ; Ornithine Decarboxylase - metabolism ; 基因表达 ; 治疗方法 ; 直肠肿瘤 ; 结肠肿瘤 ; 鸟氨酸脱羧酶</subject><ispartof>World journal of gastroenterology : WJG, 2005-04, Vol.11 (15), p.2244-2248</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2005 Baishideng Publishing Group Inc. All rights reserved. 2005</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-111288d0a313b920ed47538a5c9eab3f6d6efe6e058804810fc4e95249c4239b3</citedby><cites>FETCH-LOGICAL-c516t-111288d0a313b920ed47538a5c9eab3f6d6efe6e058804810fc4e95249c4239b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305806/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305806/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15818733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Hai-Yan</creatorcontrib><creatorcontrib>Liu, Xian-Xi</creatorcontrib><creatorcontrib>Jiang, Chun-Ying</creatorcontrib><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Liu, Shi-Lian</creatorcontrib><creatorcontrib>Bian, Ji-Feng</creatorcontrib><creatorcontrib>Wang, Xiao-Ming</creatorcontrib><creatorcontrib>Geng, Zhao</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Zhang, Bing</creatorcontrib><title>Ornithine decarboxylase gene is overexpressed in colorectal carcinoma</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma technology.METHODS: Total RNA was extracted from human colorectal cancer tissues and their normal counterpart tissues. ODC mRNA levels were examined by RT-PCR.ODC genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immunohistochemical and Western blotting assay.RESULTS: A cell line, which could steadily secrete antiODC mAb, was selected through subcloning four times. Western blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P<0.01). Immunohistochemical staining showed that colorectal carcinoma cells expressed a significantly higher level of ODC than normal colorectal mucosa (98.6±1.03% vs 5.26±5%, P<0.01).CONCLUSION: ODC gene overexpression is significantly related to human colorectal carcinoma. ODC gene expression may be a marker for the gene diagnosis and therapy of colorectal carcinoma.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Colorectal Cancer</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - physiopathology</subject><subject>Female</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Ornithine Decarboxylase - genetics</subject><subject>Ornithine Decarboxylase - immunology</subject><subject>Ornithine Decarboxylase - metabolism</subject><subject>基因表达</subject><subject>治疗方法</subject><subject>直肠肿瘤</subject><subject>结肠肿瘤</subject><subject>鸟氨酸脱羧酶</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1P3DAQhq2Kqmyhd05VhFBvWcZfiXOphBD9kJC4wNlynEnWS9Ze7OwC_x6vdtXCyZL9zOOZeQk5ozDntVCXz8thvqV07qicMybEJzJjjDYlUwKOyIwC1GXDWX1Mvqa0BGCcS_aFHFOpqKo5n5Gbu-jdtHAeiw6tiW14eR1NwmLAfOVSEbYY8WUdMSXsCucLG8YQ0U5mLDJvnQ8rc0o-92ZM-O1wnpCHXzf313_K27vff6-vbksraTWVlFKmVAeGU942DLATteTKSNugaXlfdRX2WCFIpUAoCr0V2EgmGisYb1p-Qn7uvetNu8LOop-iGfU6upWJrzoYpz--eLfQQ9hqwbMTqiy42Aueje-NH_QybKLPLeu8SgYgqcw7y9iPwz8xPG0wTXrlksVxNB7DJumqrhnUFc8g7EEbQ0oR-3-9UNC7iHZenSPSOSK9iyiXfH8_w_-CQyYZOD84F8EPTy532Rr72LsRMySBUSb5GwVAmew</recordid><startdate>20050421</startdate><enddate>20050421</enddate><creator>Hu, Hai-Yan</creator><creator>Liu, Xian-Xi</creator><creator>Jiang, Chun-Ying</creator><creator>Lu, Yi</creator><creator>Liu, Shi-Lian</creator><creator>Bian, Ji-Feng</creator><creator>Wang, Xiao-Ming</creator><creator>Geng, Zhao</creator><creator>Zhang, Yan</creator><creator>Zhang, Bing</creator><general>Experimental Center of Medical Molecular Biology, Biochemistry and Molecular Biology Institute, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China%Department of Colo-proctology, The Affiliated Hospital of Shandong University of Tradition Chinese Medicine,Jinan 250012, Shandong Province, China</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20050421</creationdate><title>Ornithine decarboxylase gene is overexpressed in colorectal carcinoma</title><author>Hu, Hai-Yan ; Liu, Xian-Xi ; Jiang, Chun-Ying ; Lu, Yi ; Liu, Shi-Lian ; Bian, Ji-Feng ; Wang, Xiao-Ming ; Geng, Zhao ; Zhang, Yan ; Zhang, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-111288d0a313b920ed47538a5c9eab3f6d6efe6e058804810fc4e95249c4239b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Colorectal Cancer</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - physiopathology</topic><topic>Female</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Ornithine Decarboxylase - genetics</topic><topic>Ornithine Decarboxylase - immunology</topic><topic>Ornithine Decarboxylase - metabolism</topic><topic>基因表达</topic><topic>治疗方法</topic><topic>直肠肿瘤</topic><topic>结肠肿瘤</topic><topic>鸟氨酸脱羧酶</topic><toplevel>online_resources</toplevel><creatorcontrib>Hu, Hai-Yan</creatorcontrib><creatorcontrib>Liu, Xian-Xi</creatorcontrib><creatorcontrib>Jiang, Chun-Ying</creatorcontrib><creatorcontrib>Lu, Yi</creatorcontrib><creatorcontrib>Liu, Shi-Lian</creatorcontrib><creatorcontrib>Bian, Ji-Feng</creatorcontrib><creatorcontrib>Wang, Xiao-Ming</creatorcontrib><creatorcontrib>Geng, Zhao</creatorcontrib><creatorcontrib>Zhang, Yan</creatorcontrib><creatorcontrib>Zhang, Bing</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Hai-Yan</au><au>Liu, Xian-Xi</au><au>Jiang, Chun-Ying</au><au>Lu, Yi</au><au>Liu, Shi-Lian</au><au>Bian, Ji-Feng</au><au>Wang, Xiao-Ming</au><au>Geng, Zhao</au><au>Zhang, Yan</au><au>Zhang, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ornithine decarboxylase gene is overexpressed in colorectal carcinoma</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2005-04-21</date><risdate>2005</risdate><volume>11</volume><issue>15</issue><spage>2244</spage><epage>2248</epage><pages>2244-2248</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM: To investigate the ornithine decarboxylase (ODC) gene expression in colorectal carcinoma, ODC mRNA was assayed by RT-PCR and ODC protein was detected by a monoclonal antibody against fusion of human colon ODC prepared by hybridoma technology.METHODS: Total RNA was extracted from human colorectal cancer tissues and their normal counterpart tissues. ODC mRNA levels were examined by RT-PCR.ODC genes amplified from RT-PCR were cloned into a prokaryotic vector pQE-30. The expressed proteins were purified by chromatography. Anti-ODC mAb was prepared with classical hybridoma techniques and used to determine the ODC expression in colon cancer tissues by immunohistochemical and Western blotting assay.RESULTS: A cell line, which could steadily secrete antiODC mAb, was selected through subcloning four times. Western blotting reconfirmed the mAb and ELISA showed that its subtype was IgG2a. RT-PCR showed that the ODC mRNA level increased greatly in colon cancer tissues (P<0.01). Immunohistochemical staining showed that colorectal carcinoma cells expressed a significantly higher level of ODC than normal colorectal mucosa (98.6±1.03% vs 5.26±5%, P<0.01).CONCLUSION: ODC gene overexpression is significantly related to human colorectal carcinoma. ODC gene expression may be a marker for the gene diagnosis and therapy of colorectal carcinoma.</abstract><cop>United States</cop><pub>Experimental Center of Medical Molecular Biology, Biochemistry and Molecular Biology Institute, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China%Department of Colo-proctology, The Affiliated Hospital of Shandong University of Tradition Chinese Medicine,Jinan 250012, Shandong Province, China</pub><pmid>15818733</pmid><doi>10.3748/wjg.v11.i15.2244</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Antibodies, Monoclonal Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Colorectal Cancer Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Colorectal Neoplasms - physiopathology Female Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Mice Mice, Inbred BALB C Ornithine Decarboxylase - genetics Ornithine Decarboxylase - immunology Ornithine Decarboxylase - metabolism 基因表达 治疗方法 直肠肿瘤 结肠肿瘤 鸟氨酸脱羧酶 |
| title | Ornithine decarboxylase gene is overexpressed in colorectal carcinoma |
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