Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences
AIM:To evaluate the efficacy of centralized culture and possible influencing factors.METHODS:From January 2010 to July 2012,66452 patients with suspected Helicobacter pylori(H.pylori) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy.Gastric...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2015-01, Vol.21 (3), p.944-952 |
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| creator | Gong, Ya-Nan Li, You-Ming Yang, Ning-Min Li, Hong-Zhang Guo, Feng Lin, Lang Wang, Qun-Ying Zhang, Jia-Kun Ji, Zi-Zhong Mao, Ji-Bo Mao, Jun-Liang Shi, Zheng-Chao Tang, Wu-Heng Zhu, Xin-Jian Shao, Wei Zhang, Xiao-Feng Wang, Xing-Hua Tong, Yue-Feng Jiang, Mi-Zu Chen, Guang-Lan Wang, Zhi-Yong Tu, Hui-Min Jiang, Guo-Fa Wu, Jian-Sheng Chen, Xu-Peng Ding, Qiu-Long Ouyang, Hong Jin, Feng-Zhe Xu, Yan-Li Zhang, Jian-Zhong |
| description | AIM:To evaluate the efficacy of centralized culture and possible influencing factors.METHODS:From January 2010 to July 2012,66452 patients with suspected Helicobacter pylori(H.pylori) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy.Gastric mucosal biopsies were taken from the antrum for culture.These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time:5,24 and 48 h.The culture results were reported after 72 h and the positive culture rates were analyzed by a χ2 test.An additional 5736 biopsies from H.pylori-positive patients(5646 rapid urease test-positive and 90 14C-urease breath test-positive) were also cultured for quality control in the central laboratory setting.RESULTS:The positive culture rate was 31.66%(21036/66452) for the patient samples and 71.72%(4114/5736) for the H.pylori-positive quality control specimens.In the 5 h transport group,the positiveculture rate was 30.99%(3865/12471),and 32.84%(14960/45553) in the 24 h transport group.In contrast,the positive culture rate declined significantly in the 48 h transport group(26.25%; P < 0.001).During transportation,the average natural temperature increased from 4.67 to 29.14℃,while the positive culture rate declined from 36.67%(1462/3987) to 24.12%(1799/7459).When the temperature exceeded 24℃,the positive culture rate decreased significantly,especially in the 48 h transport group(23.17%).CONCLUSION:Transportation of specimens within 24 h and below 24℃ is reasonable and acceptable for centralized culture of multicenter H.pylori samples. |
| doi_str_mv | 10.3748/wjg.v21.i3.944 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4299348</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>90888889504849534851485051</cqvip_id><sourcerecordid>1652413026</sourcerecordid><originalsourceid>FETCH-LOGICAL-c394t-228fc6b085d1a4e1fe99a18e9ea8f8757cc3e5f5abd4435dab1c626bf2b880da3</originalsourceid><addsrcrecordid>eNpVkU1v1DAQhi0EokvhyhHlyCXBn4l9QUKrQpEqcYGz5TjjrSvHTu2kqPx6XHZZgaWRD37m8YxehN4S3LGByw8_7w7dAyWdZ53i_BnaUUpUSyXHz9GOYDy0itHhAr0q5Q5jypigL9EFFT3lA1U7BHuIazbB_4Kp8SUFs_oUm-SaawjeptHYFXKzPIaUfeNympt5C6tfAjS2tkIujYlTUx2xLCmvjU1x8n8kPrqwQbRQXqMXzoQCb073Jfrx-er7_rq9-fbl6_7TTWuZ4mtLqXS2H7EUEzEciAOlDJGgwEgnBzFYy0A4YcaJcyYmMxLb0350dJQST4Zdoo9H77KNM0z2uJtesp9NftTJeP3_S_S3-pAeNKdKMS6r4P1JkNP9BmXVsy8WQjAR0lY06QXlhGHaV7Q7ojanUjK48zcE66dsdM1G12y0Z7pmUxve_TvcGf8bRgXYyXib4uHex8OZUVg-HSUwl1yJOqogtbAg7DfVaZ9A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1652413026</pqid></control><display><type>article</type><title>Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences</title><source>MEDLINE</source><source>Baishideng "World Journal of" online journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Gong, Ya-Nan ; Li, You-Ming ; Yang, Ning-Min ; Li, Hong-Zhang ; Guo, Feng ; Lin, Lang ; Wang, Qun-Ying ; Zhang, Jia-Kun ; Ji, Zi-Zhong ; Mao, Ji-Bo ; Mao, Jun-Liang ; Shi, Zheng-Chao ; Tang, Wu-Heng ; Zhu, Xin-Jian ; Shao, Wei ; Zhang, Xiao-Feng ; Wang, Xing-Hua ; Tong, Yue-Feng ; Jiang, Mi-Zu ; Chen, Guang-Lan ; Wang, Zhi-Yong ; Tu, Hui-Min ; Jiang, Guo-Fa ; Wu, Jian-Sheng ; Chen, Xu-Peng ; Ding, Qiu-Long ; Ouyang, Hong ; Jin, Feng-Zhe ; Xu, Yan-Li ; Zhang, Jian-Zhong</creator><creatorcontrib>Gong, Ya-Nan ; Li, You-Ming ; Yang, Ning-Min ; Li, Hong-Zhang ; Guo, Feng ; Lin, Lang ; Wang, Qun-Ying ; Zhang, Jia-Kun ; Ji, Zi-Zhong ; Mao, Ji-Bo ; Mao, Jun-Liang ; Shi, Zheng-Chao ; Tang, Wu-Heng ; Zhu, Xin-Jian ; Shao, Wei ; Zhang, Xiao-Feng ; Wang, Xing-Hua ; Tong, Yue-Feng ; Jiang, Mi-Zu ; Chen, Guang-Lan ; Wang, Zhi-Yong ; Tu, Hui-Min ; Jiang, Guo-Fa ; Wu, Jian-Sheng ; Chen, Xu-Peng ; Ding, Qiu-Long ; Ouyang, Hong ; Jin, Feng-Zhe ; Xu, Yan-Li ; Zhang, Jian-Zhong</creatorcontrib><description>AIM:To evaluate the efficacy of centralized culture and possible influencing factors.METHODS:From January 2010 to July 2012,66452 patients with suspected Helicobacter pylori(H.pylori) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy.Gastric mucosal biopsies were taken from the antrum for culture.These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time:5,24 and 48 h.The culture results were reported after 72 h and the positive culture rates were analyzed by a χ2 test.An additional 5736 biopsies from H.pylori-positive patients(5646 rapid urease test-positive and 90 14C-urease breath test-positive) were also cultured for quality control in the central laboratory setting.RESULTS:The positive culture rate was 31.66%(21036/66452) for the patient samples and 71.72%(4114/5736) for the H.pylori-positive quality control specimens.In the 5 h transport group,the positiveculture rate was 30.99%(3865/12471),and 32.84%(14960/45553) in the 24 h transport group.In contrast,the positive culture rate declined significantly in the 48 h transport group(26.25%; P &lt; 0.001).During transportation,the average natural temperature increased from 4.67 to 29.14℃,while the positive culture rate declined from 36.67%(1462/3987) to 24.12%(1799/7459).When the temperature exceeded 24℃,the positive culture rate decreased significantly,especially in the 48 h transport group(23.17%).CONCLUSION:Transportation of specimens within 24 h and below 24℃ is reasonable and acceptable for centralized culture of multicenter H.pylori samples.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v21.i3.944</identifier><identifier>PMID: 25624729</identifier><language>eng</language><publisher>United States: Baishideng Publishing Group Inc</publisher><subject>Biopsy ; Centralized ; Centralized Hospital Services - organization & administration ; China ; Clinical Trials Study ; Endoscopy, Gastrointestinal ; Feasibility Studies ; Gastric Mucosa - microbiology ; Helicobacter Infections - diagnosis ; Helicobacter Infections - microbiology ; Helicobacter pylori - isolation & purification ; Humans ; isolation;Helicobacter ; Microbial Sensitivity Tests ; Predictive Value of Tests ; pylori;Influenc ; Reproducibility of Results ; Specimen Handling - methods ; Temperature ; Time Factors ; Transportation</subject><ispartof>World journal of gastroenterology : WJG, 2015-01, Vol.21 (3), p.944-952</ispartof><rights>The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved. 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-228fc6b085d1a4e1fe99a18e9ea8f8757cc3e5f5abd4435dab1c626bf2b880da3</citedby><cites>FETCH-LOGICAL-c394t-228fc6b085d1a4e1fe99a18e9ea8f8757cc3e5f5abd4435dab1c626bf2b880da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299348/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299348/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25624729$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Ya-Nan</creatorcontrib><creatorcontrib>Li, You-Ming</creatorcontrib><creatorcontrib>Yang, Ning-Min</creatorcontrib><creatorcontrib>Li, Hong-Zhang</creatorcontrib><creatorcontrib>Guo, Feng</creatorcontrib><creatorcontrib>Lin, Lang</creatorcontrib><creatorcontrib>Wang, Qun-Ying</creatorcontrib><creatorcontrib>Zhang, Jia-Kun</creatorcontrib><creatorcontrib>Ji, Zi-Zhong</creatorcontrib><creatorcontrib>Mao, Ji-Bo</creatorcontrib><creatorcontrib>Mao, Jun-Liang</creatorcontrib><creatorcontrib>Shi, Zheng-Chao</creatorcontrib><creatorcontrib>Tang, Wu-Heng</creatorcontrib><creatorcontrib>Zhu, Xin-Jian</creatorcontrib><creatorcontrib>Shao, Wei</creatorcontrib><creatorcontrib>Zhang, Xiao-Feng</creatorcontrib><creatorcontrib>Wang, Xing-Hua</creatorcontrib><creatorcontrib>Tong, Yue-Feng</creatorcontrib><creatorcontrib>Jiang, Mi-Zu</creatorcontrib><creatorcontrib>Chen, Guang-Lan</creatorcontrib><creatorcontrib>Wang, Zhi-Yong</creatorcontrib><creatorcontrib>Tu, Hui-Min</creatorcontrib><creatorcontrib>Jiang, Guo-Fa</creatorcontrib><creatorcontrib>Wu, Jian-Sheng</creatorcontrib><creatorcontrib>Chen, Xu-Peng</creatorcontrib><creatorcontrib>Ding, Qiu-Long</creatorcontrib><creatorcontrib>Ouyang, Hong</creatorcontrib><creatorcontrib>Jin, Feng-Zhe</creatorcontrib><creatorcontrib>Xu, Yan-Li</creatorcontrib><creatorcontrib>Zhang, Jian-Zhong</creatorcontrib><title>Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM:To evaluate the efficacy of centralized culture and possible influencing factors.METHODS:From January 2010 to July 2012,66452 patients with suspected Helicobacter pylori(H.pylori) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy.Gastric mucosal biopsies were taken from the antrum for culture.These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time:5,24 and 48 h.The culture results were reported after 72 h and the positive culture rates were analyzed by a χ2 test.An additional 5736 biopsies from H.pylori-positive patients(5646 rapid urease test-positive and 90 14C-urease breath test-positive) were also cultured for quality control in the central laboratory setting.RESULTS:The positive culture rate was 31.66%(21036/66452) for the patient samples and 71.72%(4114/5736) for the H.pylori-positive quality control specimens.In the 5 h transport group,the positiveculture rate was 30.99%(3865/12471),and 32.84%(14960/45553) in the 24 h transport group.In contrast,the positive culture rate declined significantly in the 48 h transport group(26.25%; P &lt; 0.001).During transportation,the average natural temperature increased from 4.67 to 29.14℃,while the positive culture rate declined from 36.67%(1462/3987) to 24.12%(1799/7459).When the temperature exceeded 24℃,the positive culture rate decreased significantly,especially in the 48 h transport group(23.17%).CONCLUSION:Transportation of specimens within 24 h and below 24℃ is reasonable and acceptable for centralized culture of multicenter H.pylori samples.</description><subject>Biopsy</subject><subject>Centralized</subject><subject>Centralized Hospital Services - organization & administration</subject><subject>China</subject><subject>Clinical Trials Study</subject><subject>Endoscopy, Gastrointestinal</subject><subject>Feasibility Studies</subject><subject>Gastric Mucosa - microbiology</subject><subject>Helicobacter Infections - diagnosis</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Humans</subject><subject>isolation;Helicobacter</subject><subject>Microbial Sensitivity Tests</subject><subject>Predictive Value of Tests</subject><subject>pylori;Influenc</subject><subject>Reproducibility of Results</subject><subject>Specimen Handling - methods</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Transportation</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1v1DAQhi0EokvhyhHlyCXBn4l9QUKrQpEqcYGz5TjjrSvHTu2kqPx6XHZZgaWRD37m8YxehN4S3LGByw8_7w7dAyWdZ53i_BnaUUpUSyXHz9GOYDy0itHhAr0q5Q5jypigL9EFFT3lA1U7BHuIazbB_4Kp8SUFs_oUm-SaawjeptHYFXKzPIaUfeNympt5C6tfAjS2tkIujYlTUx2xLCmvjU1x8n8kPrqwQbRQXqMXzoQCb073Jfrx-er7_rq9-fbl6_7TTWuZ4mtLqXS2H7EUEzEciAOlDJGgwEgnBzFYy0A4YcaJcyYmMxLb0350dJQST4Zdoo9H77KNM0z2uJtesp9NftTJeP3_S_S3-pAeNKdKMS6r4P1JkNP9BmXVsy8WQjAR0lY06QXlhGHaV7Q7ojanUjK48zcE66dsdM1G12y0Z7pmUxve_TvcGf8bRgXYyXib4uHex8OZUVg-HSUwl1yJOqogtbAg7DfVaZ9A</recordid><startdate>20150121</startdate><enddate>20150121</enddate><creator>Gong, Ya-Nan</creator><creator>Li, You-Ming</creator><creator>Yang, Ning-Min</creator><creator>Li, Hong-Zhang</creator><creator>Guo, Feng</creator><creator>Lin, Lang</creator><creator>Wang, Qun-Ying</creator><creator>Zhang, Jia-Kun</creator><creator>Ji, Zi-Zhong</creator><creator>Mao, Ji-Bo</creator><creator>Mao, Jun-Liang</creator><creator>Shi, Zheng-Chao</creator><creator>Tang, Wu-Heng</creator><creator>Zhu, Xin-Jian</creator><creator>Shao, Wei</creator><creator>Zhang, Xiao-Feng</creator><creator>Wang, Xing-Hua</creator><creator>Tong, Yue-Feng</creator><creator>Jiang, Mi-Zu</creator><creator>Chen, Guang-Lan</creator><creator>Wang, Zhi-Yong</creator><creator>Tu, Hui-Min</creator><creator>Jiang, Guo-Fa</creator><creator>Wu, Jian-Sheng</creator><creator>Chen, Xu-Peng</creator><creator>Ding, Qiu-Long</creator><creator>Ouyang, Hong</creator><creator>Jin, Feng-Zhe</creator><creator>Xu, Yan-Li</creator><creator>Zhang, Jian-Zhong</creator><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150121</creationdate><title>Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences</title><author>Gong, Ya-Nan ; Li, You-Ming ; Yang, Ning-Min ; Li, Hong-Zhang ; Guo, Feng ; Lin, Lang ; Wang, Qun-Ying ; Zhang, Jia-Kun ; Ji, Zi-Zhong ; Mao, Ji-Bo ; Mao, Jun-Liang ; Shi, Zheng-Chao ; Tang, Wu-Heng ; Zhu, Xin-Jian ; Shao, Wei ; Zhang, Xiao-Feng ; Wang, Xing-Hua ; Tong, Yue-Feng ; Jiang, Mi-Zu ; Chen, Guang-Lan ; Wang, Zhi-Yong ; Tu, Hui-Min ; Jiang, Guo-Fa ; Wu, Jian-Sheng ; Chen, Xu-Peng ; Ding, Qiu-Long ; Ouyang, Hong ; Jin, Feng-Zhe ; Xu, Yan-Li ; Zhang, Jian-Zhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-228fc6b085d1a4e1fe99a18e9ea8f8757cc3e5f5abd4435dab1c626bf2b880da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biopsy</topic><topic>Centralized</topic><topic>Centralized Hospital Services - organization & administration</topic><topic>China</topic><topic>Clinical Trials Study</topic><topic>Endoscopy, Gastrointestinal</topic><topic>Feasibility Studies</topic><topic>Gastric Mucosa - microbiology</topic><topic>Helicobacter Infections - diagnosis</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Humans</topic><topic>isolation;Helicobacter</topic><topic>Microbial Sensitivity Tests</topic><topic>Predictive Value of Tests</topic><topic>pylori;Influenc</topic><topic>Reproducibility of Results</topic><topic>Specimen Handling - methods</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Transportation</topic><toplevel>online_resources</toplevel><creatorcontrib>Gong, Ya-Nan</creatorcontrib><creatorcontrib>Li, You-Ming</creatorcontrib><creatorcontrib>Yang, Ning-Min</creatorcontrib><creatorcontrib>Li, Hong-Zhang</creatorcontrib><creatorcontrib>Guo, Feng</creatorcontrib><creatorcontrib>Lin, Lang</creatorcontrib><creatorcontrib>Wang, Qun-Ying</creatorcontrib><creatorcontrib>Zhang, Jia-Kun</creatorcontrib><creatorcontrib>Ji, Zi-Zhong</creatorcontrib><creatorcontrib>Mao, Ji-Bo</creatorcontrib><creatorcontrib>Mao, Jun-Liang</creatorcontrib><creatorcontrib>Shi, Zheng-Chao</creatorcontrib><creatorcontrib>Tang, Wu-Heng</creatorcontrib><creatorcontrib>Zhu, Xin-Jian</creatorcontrib><creatorcontrib>Shao, Wei</creatorcontrib><creatorcontrib>Zhang, Xiao-Feng</creatorcontrib><creatorcontrib>Wang, Xing-Hua</creatorcontrib><creatorcontrib>Tong, Yue-Feng</creatorcontrib><creatorcontrib>Jiang, Mi-Zu</creatorcontrib><creatorcontrib>Chen, Guang-Lan</creatorcontrib><creatorcontrib>Wang, Zhi-Yong</creatorcontrib><creatorcontrib>Tu, Hui-Min</creatorcontrib><creatorcontrib>Jiang, Guo-Fa</creatorcontrib><creatorcontrib>Wu, Jian-Sheng</creatorcontrib><creatorcontrib>Chen, Xu-Peng</creatorcontrib><creatorcontrib>Ding, Qiu-Long</creatorcontrib><creatorcontrib>Ouyang, Hong</creatorcontrib><creatorcontrib>Jin, Feng-Zhe</creatorcontrib><creatorcontrib>Xu, Yan-Li</creatorcontrib><creatorcontrib>Zhang, Jian-Zhong</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Ya-Nan</au><au>Li, You-Ming</au><au>Yang, Ning-Min</au><au>Li, Hong-Zhang</au><au>Guo, Feng</au><au>Lin, Lang</au><au>Wang, Qun-Ying</au><au>Zhang, Jia-Kun</au><au>Ji, Zi-Zhong</au><au>Mao, Ji-Bo</au><au>Mao, Jun-Liang</au><au>Shi, Zheng-Chao</au><au>Tang, Wu-Heng</au><au>Zhu, Xin-Jian</au><au>Shao, Wei</au><au>Zhang, Xiao-Feng</au><au>Wang, Xing-Hua</au><au>Tong, Yue-Feng</au><au>Jiang, Mi-Zu</au><au>Chen, Guang-Lan</au><au>Wang, Zhi-Yong</au><au>Tu, Hui-Min</au><au>Jiang, Guo-Fa</au><au>Wu, Jian-Sheng</au><au>Chen, Xu-Peng</au><au>Ding, Qiu-Long</au><au>Ouyang, Hong</au><au>Jin, Feng-Zhe</au><au>Xu, Yan-Li</au><au>Zhang, Jian-Zhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2015-01-21</date><risdate>2015</risdate><volume>21</volume><issue>3</issue><spage>944</spage><epage>952</epage><pages>944-952</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM:To evaluate the efficacy of centralized culture and possible influencing factors.METHODS:From January 2010 to July 2012,66452 patients with suspected Helicobacter pylori(H.pylori) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy.Gastric mucosal biopsies were taken from the antrum for culture.These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time:5,24 and 48 h.The culture results were reported after 72 h and the positive culture rates were analyzed by a χ2 test.An additional 5736 biopsies from H.pylori-positive patients(5646 rapid urease test-positive and 90 14C-urease breath test-positive) were also cultured for quality control in the central laboratory setting.RESULTS:The positive culture rate was 31.66%(21036/66452) for the patient samples and 71.72%(4114/5736) for the H.pylori-positive quality control specimens.In the 5 h transport group,the positiveculture rate was 30.99%(3865/12471),and 32.84%(14960/45553) in the 24 h transport group.In contrast,the positive culture rate declined significantly in the 48 h transport group(26.25%; P &lt; 0.001).During transportation,the average natural temperature increased from 4.67 to 29.14℃,while the positive culture rate declined from 36.67%(1462/3987) to 24.12%(1799/7459).When the temperature exceeded 24℃,the positive culture rate decreased significantly,especially in the 48 h transport group(23.17%).CONCLUSION:Transportation of specimens within 24 h and below 24℃ is reasonable and acceptable for centralized culture of multicenter H.pylori samples.</abstract><cop>United States</cop><pub>Baishideng Publishing Group Inc</pub><pmid>25624729</pmid><doi>10.3748/wjg.v21.i3.944</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2015-01, Vol.21 (3), p.944-952 |
| issn | 1007-9327 2219-2840 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4299348 |
| source | MEDLINE; Baishideng "World Journal of" online journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Biopsy Centralized Centralized Hospital Services - organization & administration China Clinical Trials Study Endoscopy, Gastrointestinal Feasibility Studies Gastric Mucosa - microbiology Helicobacter Infections - diagnosis Helicobacter Infections - microbiology Helicobacter pylori - isolation & purification Humans isolation Helicobacter Microbial Sensitivity Tests Predictive Value of Tests pylori Influenc Reproducibility of Results Specimen Handling - methods Temperature Time Factors Transportation |
| title | Centralized isolation of Helicobacter pylori from multiple centers and transport condition influences |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T05%3A39%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Centralized%20isolation%20of%20Helicobacter%20pylori%20from%20multiple%20centers%20and%20transport%20condition%20influences&rft.jtitle=World%20journal%20of%20gastroenterology%20:%20WJG&rft.au=Gong,%20Ya-Nan&rft.date=2015-01-21&rft.volume=21&rft.issue=3&rft.spage=944&rft.epage=952&rft.pages=944-952&rft.issn=1007-9327&rft.eissn=2219-2840&rft_id=info:doi/10.3748/wjg.v21.i3.944&rft_dat=%3Cproquest_pubme%3E1652413026%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1652413026&rft_id=info:pmid/25624729&rft_cqvip_id=90888889504849534851485051&rfr_iscdi=true |