High-content screening technology combined with a human granuloma model as a new approach to evaluate the activities of drugs against Mycobacterium tuberculosis
Tuberculosis remains a major health problem due to the emergence of drug-resistant strains of Mycobacterium tuberculosis. Some models have provided valuable information about drug resistance and efficacy; however, the translation of these results into effective human treatments has mostly proven uns...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2015-01, Vol.59 (1), p.693-697 |
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| container_title | Antimicrobial agents and chemotherapy |
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| creator | Silva-Miranda, Mayra Ekaza, Euloge Breiman, Adrien Asehnoune, Karim Barros-Aguirre, David Pethe, Kevin Ewann, Fanny Brodin, Priscille Ballell-Pages, Lluís Altare, Frédéric |
| description | Tuberculosis remains a major health problem due to the emergence of drug-resistant strains of Mycobacterium tuberculosis. Some models have provided valuable information about drug resistance and efficacy; however, the translation of these results into effective human treatments has mostly proven unsuccessful. In this study, we adapted high-content screening (HCS) technology to investigate the activities of antitubercular compounds in the context of an in vitro granuloma model. We observed significant shifts in the MIC50s between the activities of the compounds under extracellular and granuloma conditions. |
| doi_str_mv | 10.1128/AAC.03705-14 |
| format | Article |
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All Rights Reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2015, American Society for Microbiology. 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| ispartof | Antimicrobial agents and chemotherapy, 2015-01, Vol.59 (1), p.693-697 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antitubercular Agents Antitubercular Agents - pharmacology Dose-Response Relationship, Drug Granuloma Granuloma - drug therapy High-Throughput Screening Assays High-Throughput Screening Assays - methods Humans Immunology Life Sciences Microbial Sensitivity Tests Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Susceptibility Tuberculosis, Pulmonary - drug therapy |
| title | High-content screening technology combined with a human granuloma model as a new approach to evaluate the activities of drugs against Mycobacterium tuberculosis |
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