The resistome of Pseudomonas aeruginosa in relationship to phenotypic susceptibility
Many clinical isolates of Pseudomonas aeruginosa cause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates of P. aeruginosa, obtained from diverse...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2015-01, Vol.59 (1), p.427-436 |
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| creator | Kos, Veronica N Déraspe, Maxime McLaughlin, Robert E Whiteaker, James D Roy, Paul H Alm, Richard A Corbeil, Jacques Gardner, Humphrey |
| description | Many clinical isolates of Pseudomonas aeruginosa cause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates of P. aeruginosa, obtained from diverse geographic locations collected between 2003 and 2012 and were related to microbiological susceptibility data for meropenem, levofloxacin, and amikacin. β-Lactamases and integron cassette arrangements were enriched in the established multidrug-resistant lineages of sequence types ST111 (predominantly O12) and ST235 (O11). This study demonstrates the utility of next-generation sequencing (NGS) in defining relevant resistance elements and highlights the diversity of resistance determinants within P. aeruginosa. This information is valuable in furthering the design of diagnostics and therapeutics for the treatment of P. aeruginosa infections. |
| doi_str_mv | 10.1128/AAC.03954-14 |
| format | Article |
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Key genetic determinants of resistance were identified through genome sequences of 390 clinical isolates of P. aeruginosa, obtained from diverse geographic locations collected between 2003 and 2012 and were related to microbiological susceptibility data for meropenem, levofloxacin, and amikacin. β-Lactamases and integron cassette arrangements were enriched in the established multidrug-resistant lineages of sequence types ST111 (predominantly O12) and ST235 (O11). This study demonstrates the utility of next-generation sequencing (NGS) in defining relevant resistance elements and highlights the diversity of resistance determinants within P. aeruginosa. 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All Rights Reserved.</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a490t-f6b0296c6d0f2920853acc139d66ce36f29d2281d77d4a1c1f0328437eca1f8f3</citedby><cites>FETCH-LOGICAL-a490t-f6b0296c6d0f2920853acc139d66ce36f29d2281d77d4a1c1f0328437eca1f8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291382/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4291382/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25367914$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kos, Veronica N</creatorcontrib><creatorcontrib>Déraspe, Maxime</creatorcontrib><creatorcontrib>McLaughlin, Robert E</creatorcontrib><creatorcontrib>Whiteaker, James D</creatorcontrib><creatorcontrib>Roy, Paul H</creatorcontrib><creatorcontrib>Alm, Richard A</creatorcontrib><creatorcontrib>Corbeil, Jacques</creatorcontrib><creatorcontrib>Gardner, Humphrey</creatorcontrib><title>The resistome of Pseudomonas aeruginosa in relationship to phenotypic susceptibility</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>Many clinical isolates of Pseudomonas aeruginosa cause infections that are difficult to eradicate due to their resistance to a wide variety of antibiotics. 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| ispartof | Antimicrobial agents and chemotherapy, 2015-01, Vol.59 (1), p.427-436 |
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| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4291382 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Amikacin Amikacin - pharmacology Amikacin - therapeutic use Anti-Bacterial Agents Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Bacterial Typing Techniques Base Sequence beta-Lactamases - genetics DNA, Bacterial - genetics Drug Resistance, Multiple, Bacterial - genetics Genome, Bacterial - genetics Humans Levofloxacin Levofloxacin - pharmacology Levofloxacin - therapeutic use Mechanisms of Resistance Microbial Sensitivity Tests Multilocus Sequence Typing Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - isolation & purification Pseudomonas Infections - drug therapy Pseudomonas Infections - microbiology Sequence Analysis, DNA Thienamycins Thienamycins - pharmacology Thienamycins - therapeutic use |
| title | The resistome of Pseudomonas aeruginosa in relationship to phenotypic susceptibility |
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