Limited polymorphisms in k13 gene in Plasmodium falciparum isolates from Dakar, Senegal in 2012-2013
The emergence of Plasmodium falciparum resistance to artemisinin and its derivatives, manifested as delayed parasite clearance following the treatment, has developed in Southeast Asia. The spread of resistance to artemisinin from Asia to Africa may be catastrophic for malaria control and elimination...
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| creator | Torrentino-Madamet, Marylin Fall, Bécaye Benoit, Nicolas Camara, Cheikhou Amalvict, Rémy Fall, Mansour Dionne, Pierre Ba Fall, Kadidiatou Nakoulima, Aminata Diatta, Bakary Diemé, Yaya Ménard, Didier Wade, Boubacar Pradines, Bruno |
| description | The emergence of Plasmodium falciparum resistance to artemisinin and its derivatives, manifested as delayed parasite clearance following the treatment, has developed in Southeast Asia. The spread of resistance to artemisinin from Asia to Africa may be catastrophic for malaria control and elimination worldwide. Recently, mutations in the propeller domain of the Kelch 13 (k13) gene (PF3D71343700) were associated with in vitro resistance to artemisinin and with delayed clearance after artemisinin treatment in southern Asia. The aim of the study was to characterize the genetic variability of k13 and to evaluate the molecular resistance to artemisinin for the first time in Senegal.
Plasmodium falciparum isolates were collected from 138 malaria patients in Dakar and its districts during the rainy season of October 2012 to January 2013 at the Hôpital Principal de Dakar. The k13 gene was amplified using nested PCR and sequenced.
A very limited variability within the k13 gene in Senegalese P. falciparum isolates was identified. No polymorphism was detected in the six k13-propeller blades. Only two mutations, T149S (6.3%) and K189T (42.2%), and one (N) or two (NN) asparagine insertion at the codon 142 (4.7 and 6.3%, respectively) were detected in the Plasmodium/Apicomplexa-specific domain. None of the polymorphisms associated with artemisinin resistance in Southeast Asia was detected in the 138 P. falciparum from Dakar.
The present data do not suggest widespread artemisinin resistance in Dakar in 2012-2013. Notably, the C580Y, R539T or Y493H substitutions that were associated with in vitro resistance or delayed parasite clearance in Southeast Asia were not observed in Dakar, nor were any of the polymorphisms observed in parasites from Southeast Asia, nor the M476I mutation that was selected in vitro with artemisinin pressure in a African parasite line. |
| doi_str_mv | 10.1186/1475-2875-13-472 |
| format | Article |
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Plasmodium falciparum isolates were collected from 138 malaria patients in Dakar and its districts during the rainy season of October 2012 to January 2013 at the Hôpital Principal de Dakar. The k13 gene was amplified using nested PCR and sequenced.
A very limited variability within the k13 gene in Senegalese P. falciparum isolates was identified. No polymorphism was detected in the six k13-propeller blades. Only two mutations, T149S (6.3%) and K189T (42.2%), and one (N) or two (NN) asparagine insertion at the codon 142 (4.7 and 6.3%, respectively) were detected in the Plasmodium/Apicomplexa-specific domain. None of the polymorphisms associated with artemisinin resistance in Southeast Asia was detected in the 138 P. falciparum from Dakar.
The present data do not suggest widespread artemisinin resistance in Dakar in 2012-2013. Notably, the C580Y, R539T or Y493H substitutions that were associated with in vitro resistance or delayed parasite clearance in Southeast Asia were not observed in Dakar, nor were any of the polymorphisms observed in parasites from Southeast Asia, nor the M476I mutation that was selected in vitro with artemisinin pressure in a African parasite line.</description><identifier>ISSN: 1475-2875</identifier><identifier>EISSN: 1475-2875</identifier><identifier>DOI: 10.1186/1475-2875-13-472</identifier><identifier>PMID: 25471113</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Amino Acid Substitution ; Analysis ; Antimalarials ; Antimalarials - pharmacology ; Artemisinins ; Artemisinins - pharmacology ; Codon ; DNA, Protozoan ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Drug Resistance ; Genetic aspects ; Genetic polymorphisms ; Genetic research ; Genotype ; Health aspects ; Human health and pathology ; Humans ; Infectious diseases ; Lactones ; Lactones - pharmacology ; Life Sciences ; Malaria ; Malaria, Falciparum ; Malaria, Falciparum - parasitology ; Mutation, Missense ; Plasmodium falciparum ; Plasmodium falciparum - classification ; Plasmodium falciparum - drug effects ; Plasmodium falciparum - genetics ; Plasmodium falciparum - isolation & purification ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Protozoan Proteins ; Protozoan Proteins - genetics ; Senegal ; Sequence Analysis, DNA</subject><ispartof>Malaria journal, 2014-12, Vol.13 (1), p.472-472, Article 472</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>Attribution</rights><rights>Torrentino-Madamet et al.; licensee BioMed Central Ltd. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-a3cb4cc94ac1c3398cdb6b033da873e05a90a469f7887cb26a4ef26f268f2b853</citedby><cites>FETCH-LOGICAL-c596t-a3cb4cc94ac1c3398cdb6b033da873e05a90a469f7887cb26a4ef26f268f2b853</cites><orcidid>0000-0003-1357-4495</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289025/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289025/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25471113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02560659$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Torrentino-Madamet, Marylin</creatorcontrib><creatorcontrib>Fall, Bécaye</creatorcontrib><creatorcontrib>Benoit, Nicolas</creatorcontrib><creatorcontrib>Camara, Cheikhou</creatorcontrib><creatorcontrib>Amalvict, Rémy</creatorcontrib><creatorcontrib>Fall, Mansour</creatorcontrib><creatorcontrib>Dionne, Pierre</creatorcontrib><creatorcontrib>Ba Fall, Kadidiatou</creatorcontrib><creatorcontrib>Nakoulima, Aminata</creatorcontrib><creatorcontrib>Diatta, Bakary</creatorcontrib><creatorcontrib>Diemé, Yaya</creatorcontrib><creatorcontrib>Ménard, Didier</creatorcontrib><creatorcontrib>Wade, Boubacar</creatorcontrib><creatorcontrib>Pradines, Bruno</creatorcontrib><title>Limited polymorphisms in k13 gene in Plasmodium falciparum isolates from Dakar, Senegal in 2012-2013</title><title>Malaria journal</title><addtitle>Malar J</addtitle><description>The emergence of Plasmodium falciparum resistance to artemisinin and its derivatives, manifested as delayed parasite clearance following the treatment, has developed in Southeast Asia. The spread of resistance to artemisinin from Asia to Africa may be catastrophic for malaria control and elimination worldwide. Recently, mutations in the propeller domain of the Kelch 13 (k13) gene (PF3D71343700) were associated with in vitro resistance to artemisinin and with delayed clearance after artemisinin treatment in southern Asia. The aim of the study was to characterize the genetic variability of k13 and to evaluate the molecular resistance to artemisinin for the first time in Senegal.
Plasmodium falciparum isolates were collected from 138 malaria patients in Dakar and its districts during the rainy season of October 2012 to January 2013 at the Hôpital Principal de Dakar. The k13 gene was amplified using nested PCR and sequenced.
A very limited variability within the k13 gene in Senegalese P. falciparum isolates was identified. No polymorphism was detected in the six k13-propeller blades. Only two mutations, T149S (6.3%) and K189T (42.2%), and one (N) or two (NN) asparagine insertion at the codon 142 (4.7 and 6.3%, respectively) were detected in the Plasmodium/Apicomplexa-specific domain. None of the polymorphisms associated with artemisinin resistance in Southeast Asia was detected in the 138 P. falciparum from Dakar.
The present data do not suggest widespread artemisinin resistance in Dakar in 2012-2013. Notably, the C580Y, R539T or Y493H substitutions that were associated with in vitro resistance or delayed parasite clearance in Southeast Asia were not observed in Dakar, nor were any of the polymorphisms observed in parasites from Southeast Asia, nor the M476I mutation that was selected in vitro with artemisinin pressure in a African parasite line.</description><subject>Amino Acid Substitution</subject><subject>Analysis</subject><subject>Antimalarials</subject><subject>Antimalarials - pharmacology</subject><subject>Artemisinins</subject><subject>Artemisinins - pharmacology</subject><subject>Codon</subject><subject>DNA, Protozoan</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Drug Resistance</subject><subject>Genetic aspects</subject><subject>Genetic polymorphisms</subject><subject>Genetic research</subject><subject>Genotype</subject><subject>Health aspects</subject><subject>Human health and pathology</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Lactones</subject><subject>Lactones - pharmacology</subject><subject>Life Sciences</subject><subject>Malaria</subject><subject>Malaria, Falciparum</subject><subject>Malaria, Falciparum - parasitology</subject><subject>Mutation, Missense</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - classification</subject><subject>Plasmodium falciparum - drug effects</subject><subject>Plasmodium falciparum - genetics</subject><subject>Plasmodium falciparum - isolation & purification</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Protozoan Proteins</subject><subject>Protozoan Proteins - genetics</subject><subject>Senegal</subject><subject>Sequence Analysis, DNA</subject><issn>1475-2875</issn><issn>1475-2875</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1r3DAQhkVoSdI095yKoZcU6lTSyLJ0KSz5aAoLKTQ5C1mWd9VYlit5A_n3ldl0SUoPQWI0jN5n9MGL0AnBZ4QI_oWwuiqpyIFAyWq6hw53pTfP8gP0LqVfGJNa1HQfHdCK1YQQOETt0nk32bYYQ__oQxzXLvlUuKG4J1Cs7GDn_Eevkw-t2_ii071xo445dSn0erKp6GLwxYW-1_Fz8TMjK93PFMWEljnAe_Q2Y8keP61H6O7q8vb8ulzefPt-vliWppJ8KjWYhhkjmTbEAEhh2oY3GKDVogaLKy2xZlx2tRC1aSjXzHaU5yk62ogKjtDXbd9x03jbGjtMUfdqjM7r-KiCdurlzuDWahUeFKNCYjo3-LRtsP4Hu14s1VzLIo55JR9I1p4-HRbD741Nk_IuGdv3erBhkxThjHLCgPNXSEEyyStOs_TjVpr_0Co3dCFf1MxytahAcmAYZFad_UeVR2u9M2Gwncv1FwDeAiaGlKLtdq8jWM1WUrNX1OwVRUBlK2Xkw_PP3AF_vQN_AD0awMc</recordid><startdate>20141204</startdate><enddate>20141204</enddate><creator>Torrentino-Madamet, Marylin</creator><creator>Fall, Bécaye</creator><creator>Benoit, Nicolas</creator><creator>Camara, Cheikhou</creator><creator>Amalvict, Rémy</creator><creator>Fall, Mansour</creator><creator>Dionne, Pierre</creator><creator>Ba Fall, Kadidiatou</creator><creator>Nakoulima, Aminata</creator><creator>Diatta, Bakary</creator><creator>Diemé, Yaya</creator><creator>Ménard, Didier</creator><creator>Wade, Boubacar</creator><creator>Pradines, Bruno</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1357-4495</orcidid></search><sort><creationdate>20141204</creationdate><title>Limited polymorphisms in k13 gene in Plasmodium falciparum isolates from Dakar, Senegal in 2012-2013</title><author>Torrentino-Madamet, Marylin ; Fall, Bécaye ; Benoit, Nicolas ; Camara, Cheikhou ; Amalvict, Rémy ; Fall, Mansour ; Dionne, Pierre ; Ba Fall, Kadidiatou ; Nakoulima, Aminata ; Diatta, Bakary ; Diemé, Yaya ; Ménard, Didier ; Wade, Boubacar ; Pradines, Bruno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-a3cb4cc94ac1c3398cdb6b033da873e05a90a469f7887cb26a4ef26f268f2b853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Amino Acid Substitution</topic><topic>Analysis</topic><topic>Antimalarials</topic><topic>Antimalarials - pharmacology</topic><topic>Artemisinins</topic><topic>Artemisinins - pharmacology</topic><topic>Codon</topic><topic>DNA, Protozoan</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Drug Resistance</topic><topic>Genetic aspects</topic><topic>Genetic polymorphisms</topic><topic>Genetic research</topic><topic>Genotype</topic><topic>Health aspects</topic><topic>Human health and pathology</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Lactones</topic><topic>Lactones - pharmacology</topic><topic>Life Sciences</topic><topic>Malaria</topic><topic>Malaria, Falciparum</topic><topic>Malaria, Falciparum - parasitology</topic><topic>Mutation, Missense</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - classification</topic><topic>Plasmodium falciparum - drug effects</topic><topic>Plasmodium falciparum - genetics</topic><topic>Plasmodium falciparum - isolation & purification</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Protozoan Proteins</topic><topic>Protozoan Proteins - genetics</topic><topic>Senegal</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torrentino-Madamet, Marylin</creatorcontrib><creatorcontrib>Fall, Bécaye</creatorcontrib><creatorcontrib>Benoit, Nicolas</creatorcontrib><creatorcontrib>Camara, Cheikhou</creatorcontrib><creatorcontrib>Amalvict, Rémy</creatorcontrib><creatorcontrib>Fall, Mansour</creatorcontrib><creatorcontrib>Dionne, Pierre</creatorcontrib><creatorcontrib>Ba Fall, Kadidiatou</creatorcontrib><creatorcontrib>Nakoulima, Aminata</creatorcontrib><creatorcontrib>Diatta, Bakary</creatorcontrib><creatorcontrib>Diemé, Yaya</creatorcontrib><creatorcontrib>Ménard, Didier</creatorcontrib><creatorcontrib>Wade, Boubacar</creatorcontrib><creatorcontrib>Pradines, Bruno</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Malaria journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torrentino-Madamet, Marylin</au><au>Fall, Bécaye</au><au>Benoit, Nicolas</au><au>Camara, Cheikhou</au><au>Amalvict, Rémy</au><au>Fall, Mansour</au><au>Dionne, Pierre</au><au>Ba Fall, Kadidiatou</au><au>Nakoulima, Aminata</au><au>Diatta, Bakary</au><au>Diemé, Yaya</au><au>Ménard, Didier</au><au>Wade, Boubacar</au><au>Pradines, Bruno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Limited polymorphisms in k13 gene in Plasmodium falciparum isolates from Dakar, Senegal in 2012-2013</atitle><jtitle>Malaria journal</jtitle><addtitle>Malar J</addtitle><date>2014-12-04</date><risdate>2014</risdate><volume>13</volume><issue>1</issue><spage>472</spage><epage>472</epage><pages>472-472</pages><artnum>472</artnum><issn>1475-2875</issn><eissn>1475-2875</eissn><abstract>The emergence of Plasmodium falciparum resistance to artemisinin and its derivatives, manifested as delayed parasite clearance following the treatment, has developed in Southeast Asia. The spread of resistance to artemisinin from Asia to Africa may be catastrophic for malaria control and elimination worldwide. Recently, mutations in the propeller domain of the Kelch 13 (k13) gene (PF3D71343700) were associated with in vitro resistance to artemisinin and with delayed clearance after artemisinin treatment in southern Asia. The aim of the study was to characterize the genetic variability of k13 and to evaluate the molecular resistance to artemisinin for the first time in Senegal.
Plasmodium falciparum isolates were collected from 138 malaria patients in Dakar and its districts during the rainy season of October 2012 to January 2013 at the Hôpital Principal de Dakar. The k13 gene was amplified using nested PCR and sequenced.
A very limited variability within the k13 gene in Senegalese P. falciparum isolates was identified. No polymorphism was detected in the six k13-propeller blades. Only two mutations, T149S (6.3%) and K189T (42.2%), and one (N) or two (NN) asparagine insertion at the codon 142 (4.7 and 6.3%, respectively) were detected in the Plasmodium/Apicomplexa-specific domain. None of the polymorphisms associated with artemisinin resistance in Southeast Asia was detected in the 138 P. falciparum from Dakar.
The present data do not suggest widespread artemisinin resistance in Dakar in 2012-2013. Notably, the C580Y, R539T or Y493H substitutions that were associated with in vitro resistance or delayed parasite clearance in Southeast Asia were not observed in Dakar, nor were any of the polymorphisms observed in parasites from Southeast Asia, nor the M476I mutation that was selected in vitro with artemisinin pressure in a African parasite line.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25471113</pmid><doi>10.1186/1475-2875-13-472</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1357-4495</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Amino Acid Substitution Analysis Antimalarials Antimalarials - pharmacology Artemisinins Artemisinins - pharmacology Codon DNA, Protozoan DNA, Protozoan - chemistry DNA, Protozoan - genetics Drug Resistance Genetic aspects Genetic polymorphisms Genetic research Genotype Health aspects Human health and pathology Humans Infectious diseases Lactones Lactones - pharmacology Life Sciences Malaria Malaria, Falciparum Malaria, Falciparum - parasitology Mutation, Missense Plasmodium falciparum Plasmodium falciparum - classification Plasmodium falciparum - drug effects Plasmodium falciparum - genetics Plasmodium falciparum - isolation & purification Polymerase Chain Reaction Polymorphism, Genetic Protozoan Proteins Protozoan Proteins - genetics Senegal Sequence Analysis, DNA |
| title | Limited polymorphisms in k13 gene in Plasmodium falciparum isolates from Dakar, Senegal in 2012-2013 |
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