RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress
Summary Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcrip...
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Veröffentlicht in: | Molecular microbiology 2013-09, Vol.89 (5), p.975-988 |
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description | Summary
Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts. |
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Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/mmi.12324</identifier><identifier>PMID: 23841692</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Arginine - metabolism ; Aspergillus nidulans - genetics ; Fungi ; Gene Deletion ; Gene expression ; Gene Expression Regulation ; Genotype & phenotype ; Glutamine - metabolism ; Mutation ; Nitrogen ; Nitrogen - metabolism ; Oxidative Stress ; Ribonucleic acid ; RNA ; RNA Stability ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism</subject><ispartof>Molecular microbiology, 2013-09, Vol.89 (5), p.975-988</ispartof><rights>2013 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.</rights><rights>Copyright Blackwell Publishing Ltd. Sep 2013</rights><rights>2013 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4434-4f60ced45ce56cd9198f31e7a8a228a84d94c4d58cb8ca76632d4c6cdc7a58ce3</citedby><cites>FETCH-LOGICAL-c4434-4f60ced45ce56cd9198f31e7a8a228a84d94c4d58cb8ca76632d4c6cdc7a58ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fmmi.12324$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fmmi.12324$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,315,781,785,886,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23841692$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krol, Kinga</creatorcontrib><creatorcontrib>Morozov, Igor Y.</creatorcontrib><creatorcontrib>Jones, Meriel G.</creatorcontrib><creatorcontrib>Wyszomirski, Tomasz</creatorcontrib><creatorcontrib>Weglenski, Piotr</creatorcontrib><creatorcontrib>Dzikowska, Agnieszka</creatorcontrib><creatorcontrib>Caddick, Mark X.</creatorcontrib><title>RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.</description><subject>Arginine - metabolism</subject><subject>Aspergillus nidulans - genetics</subject><subject>Fungi</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genotype & phenotype</subject><subject>Glutamine - metabolism</subject><subject>Mutation</subject><subject>Nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>Oxidative Stress</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Stability</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kUtLKzEYhoMc0XpZ-AcOAVcuRnObaWYjSPEGiiAK7kKa-aaNzCRzklTtvze1Kro42QSS53vyhhehA0qOaV4nfW-PKeNMbKAR5VVZsLqUf9CI1CUpuGRP22gnxmdCKCcV30LbjEtBq5qNUH8f-jMcYLbodIKI0xxwTHpqO5uW2Lc4DmBsaw1OQbtogh1SxNblkTh4FwEnj6c-zbGzKfgZOBz9IhjA2jXYv9lGJ_uycuaBuIc2W91F2P_cd9HjxfnD5Kq4ubu8npzdFEYILgrRVsRAI0oDZWWamtay5RTGWmrGpJaiqYURTSnNVBo9rirOGmEyacY6HwLfRadr77CY9tAYcDl9p4Zgex2Wymurft84O1cz_6IEk4yPaRYcfgqC_7eAmNRz_pXLmRUVnJSEltWKOlpTJvgYA7TfL1CiVs2o3Iz6aCazf39G-ia_qsjAyRp4tR0s_29St7fXa-U7QMiboQ</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Krol, Kinga</creator><creator>Morozov, Igor Y.</creator><creator>Jones, Meriel G.</creator><creator>Wyszomirski, Tomasz</creator><creator>Weglenski, Piotr</creator><creator>Dzikowska, Agnieszka</creator><creator>Caddick, Mark X.</creator><general>Blackwell Publishing Ltd</general><general>BlackWell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>201309</creationdate><title>RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress</title><author>Krol, Kinga ; Morozov, Igor Y. ; Jones, Meriel G. ; Wyszomirski, Tomasz ; Weglenski, Piotr ; Dzikowska, Agnieszka ; Caddick, Mark X.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4434-4f60ced45ce56cd9198f31e7a8a228a84d94c4d58cb8ca76632d4c6cdc7a58ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Arginine - metabolism</topic><topic>Aspergillus nidulans - genetics</topic><topic>Fungi</topic><topic>Gene Deletion</topic><topic>Gene expression</topic><topic>Gene Expression Regulation</topic><topic>Genotype & phenotype</topic><topic>Glutamine - metabolism</topic><topic>Mutation</topic><topic>Nitrogen</topic><topic>Nitrogen - metabolism</topic><topic>Oxidative Stress</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Stability</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krol, Kinga</creatorcontrib><creatorcontrib>Morozov, Igor Y.</creatorcontrib><creatorcontrib>Jones, Meriel G.</creatorcontrib><creatorcontrib>Wyszomirski, Tomasz</creatorcontrib><creatorcontrib>Weglenski, Piotr</creatorcontrib><creatorcontrib>Dzikowska, Agnieszka</creatorcontrib><creatorcontrib>Caddick, Mark X.</creatorcontrib><collection>Wiley-Blackwell Open Access Titles</collection><collection>Wiley Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krol, Kinga</au><au>Morozov, Igor Y.</au><au>Jones, Meriel G.</au><au>Wyszomirski, Tomasz</au><au>Weglenski, Piotr</au><au>Dzikowska, Agnieszka</au><au>Caddick, Mark X.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2013-09</date><risdate>2013</risdate><volume>89</volume><issue>5</issue><spage>975</spage><epage>988</epage><pages>975-988</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23841692</pmid><doi>10.1111/mmi.12324</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Arginine - metabolism Aspergillus nidulans - genetics Fungi Gene Deletion Gene expression Gene Expression Regulation Genotype & phenotype Glutamine - metabolism Mutation Nitrogen Nitrogen - metabolism Oxidative Stress Ribonucleic acid RNA RNA Stability RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism |
title | RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress |
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