RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress

Summary Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcrip...

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Veröffentlicht in:Molecular microbiology 2013-09, Vol.89 (5), p.975-988
Hauptverfasser: Krol, Kinga, Morozov, Igor Y., Jones, Meriel G., Wyszomirski, Tomasz, Weglenski, Piotr, Dzikowska, Agnieszka, Caddick, Mark X.
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container_end_page 988
container_issue 5
container_start_page 975
container_title Molecular microbiology
container_volume 89
creator Krol, Kinga
Morozov, Igor Y.
Jones, Meriel G.
Wyszomirski, Tomasz
Weglenski, Piotr
Dzikowska, Agnieszka
Caddick, Mark X.
description Summary Differential regulation of transcript stability is an effective means by which an organism can modulate gene expression. A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.
doi_str_mv 10.1111/mmi.12324
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A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/mmi.12324</identifier><identifier>PMID: 23841692</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Arginine - metabolism ; Aspergillus nidulans - genetics ; Fungi ; Gene Deletion ; Gene expression ; Gene Expression Regulation ; Genotype &amp; phenotype ; Glutamine - metabolism ; Mutation ; Nitrogen ; Nitrogen - metabolism ; Oxidative Stress ; Ribonucleic acid ; RNA ; RNA Stability ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism</subject><ispartof>Molecular microbiology, 2013-09, Vol.89 (5), p.975-988</ispartof><rights>2013 The Authors. Molecular Microbiology published by John Wiley &amp; Sons Ltd.</rights><rights>Copyright Blackwell Publishing Ltd. Sep 2013</rights><rights>2013 The Authors. 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A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.</description><subject>Arginine - metabolism</subject><subject>Aspergillus nidulans - genetics</subject><subject>Fungi</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>Gene Expression Regulation</subject><subject>Genotype &amp; phenotype</subject><subject>Glutamine - metabolism</subject><subject>Mutation</subject><subject>Nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>Oxidative Stress</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Stability</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kUtLKzEYhoMc0XpZ-AcOAVcuRnObaWYjSPEGiiAK7kKa-aaNzCRzklTtvze1Kro42QSS53vyhhehA0qOaV4nfW-PKeNMbKAR5VVZsLqUf9CI1CUpuGRP22gnxmdCKCcV30LbjEtBq5qNUH8f-jMcYLbodIKI0xxwTHpqO5uW2Lc4DmBsaw1OQbtogh1SxNblkTh4FwEnj6c-zbGzKfgZOBz9IhjA2jXYv9lGJ_uycuaBuIc2W91F2P_cd9HjxfnD5Kq4ubu8npzdFEYILgrRVsRAI0oDZWWamtay5RTGWmrGpJaiqYURTSnNVBo9rirOGmEyacY6HwLfRadr77CY9tAYcDl9p4Zgex2Wymurft84O1cz_6IEk4yPaRYcfgqC_7eAmNRz_pXLmRUVnJSEltWKOlpTJvgYA7TfL1CiVs2o3Iz6aCazf39G-ia_qsjAyRp4tR0s_29St7fXa-U7QMiboQ</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Krol, Kinga</creator><creator>Morozov, Igor Y.</creator><creator>Jones, Meriel G.</creator><creator>Wyszomirski, Tomasz</creator><creator>Weglenski, Piotr</creator><creator>Dzikowska, Agnieszka</creator><creator>Caddick, Mark X.</creator><general>Blackwell Publishing Ltd</general><general>BlackWell Publishing Ltd</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>201309</creationdate><title>RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress</title><author>Krol, Kinga ; 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A well‐characterized example is glutamine signalled degradation of specific transcripts in Aspergillus nidulans. In the case of areA, which encodes a wide‐domain transcription factor mediating nitrogen metabolite repression, the signal is mediated through a highly conserved region of the 3′ UTR. Utilizing this RNA sequence we isolated RrmA, an RNA recognition motif protein. Disruption of the respective gene led to loss of both glutamine signalled transcript degradation as well as nitrate signalled stabilization of niaD mRNA. However, nitrogen starvation was shown to act independently of RrmA in stabilizing certain transcripts. RrmA was also implicated in the regulation of arginine catabolism gene expression and the oxidative stress responses at the level of mRNA stability. ΔrrmA mutants are hypersensitive to oxidative stress. This phenotype correlates with destabilization of eifE and dhsA mRNA. eifE encodes eIF5A, a translation factor within which a conserved lysine is post‐translationally modified to hypusine, a process requiring DhsA. Intriguingly, for specific transcripts RrmA mediates both stabilization and destabilization and the specificity of the signals transduced is transcript dependent, suggesting it acts in consort with other factors which differ between transcripts.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23841692</pmid><doi>10.1111/mmi.12324</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
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subjects Arginine - metabolism
Aspergillus nidulans - genetics
Fungi
Gene Deletion
Gene expression
Gene Expression Regulation
Genotype & phenotype
Glutamine - metabolism
Mutation
Nitrogen
Nitrogen - metabolism
Oxidative Stress
Ribonucleic acid
RNA
RNA Stability
RNA-Binding Proteins - genetics
RNA-Binding Proteins - metabolism
title RrmA regulates the stability of specific transcripts in response to both nitrogen source and oxidative stress
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