Three‐dimensional image cytometer based on widefield structured light microscopy and high‐speed remote depth scanning
A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth‐resolved image can be rapidly generated using a structured light re...
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| Veröffentlicht in: | Cytometry. Part A 2015-01, Vol.87 (1), p.49-60 |
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| container_title | Cytometry. Part A |
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| creator | Choi, Heejin Wadduwage, Dushan N. Tu, Ting Yuan Matsudaira, Paul So, Peter T. C. |
| description | A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth‐resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. Finally, the large field of view, high NA objective lens and the high pixelation, high frame rate sCMOS camera enable high resolution, high sensitivity imaging of a large cell population. This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:105. © 2014 International Society for Advancement of Cytometry |
| doi_str_mv | 10.1002/cyto.a.22584 |
| format | Article |
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This system can image at 800 cell/sec in 3D at submicron resolution corresponding to imaging 1 million cells in 20 min. The statistical accuracy of this instrument is verified by quantitatively measuring rare cell populations with ratio ranging from 1:1 to 1:105. © 2014 International Society for Advancement of Cytometry</description><subject>3D image cytometry</subject><subject>Algorithms</subject><subject>Animals</subject><subject>Fibroblasts - ultrastructure</subject><subject>Fluorescent Dyes</subject><subject>Image Cytometry - instrumentation</subject><subject>Image Cytometry - methods</subject><subject>Imaging, Three-Dimensional - instrumentation</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Kidney - ultrastructure</subject><subject>Lenses</subject><subject>Light</subject><subject>Lighting</subject><subject>Mice</subject><subject>Microscopy - instrumentation</subject><subject>Microscopy - methods</subject><subject>Muntjacs</subject><subject>rare cell detection</subject><subject>remote depth scanning</subject><subject>structured light illumination</subject><subject>Time Factors</subject><issn>1552-4922</issn><issn>1552-4930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkT1vFDEQhi0EIiHQUSOXFNzhr_1qkKITAaRIaY6CyvLZ41ujXXuxvYm24yfwG_NL8HHhBA2iGmv86NHMvAi9pGRNCWFv9ZLDWq0Zq1rxCJ3TqmIr0XHy-PRm7Aw9S-krIbwinD1FZ6ziFaNtc46WbR8B7r__MG4En1zwasBuVHvAB_EIGSLeqQQGB4_vnAHrYDA45TjrPMfSH9y-z3h0Ooakw7Rg5Q3uS7NY0wSFiDCGDNjAlHuctPLe-f1z9MSqIcGLh3qBPl-9324-rq5vPnzaXF6vtKiZWFlDOkJ3dacoZ9BZI2jLaGdYs2ttZxRjjbDEGKEtJ6rVjagJ13XVcGNNLTS_QO-O3mnejWA0-BzVIKdYtoyLDMrJv3-86-U-3ErBWsLqrghePwhi-DZDynJ0ScMwKA9hTpLWoiG0EQ37H7QE0JKOFvTNET2cLUWwp4kokYdg5eH-UslfwRb81Z9bnODfSRZAHIE7N8DyT5ncfNneXB69PwGzA7VH</recordid><startdate>201501</startdate><enddate>201501</enddate><creator>Choi, Heejin</creator><creator>Wadduwage, Dushan N.</creator><creator>Tu, Ting Yuan</creator><creator>Matsudaira, Paul</creator><creator>So, Peter T. 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C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4624-fd0901b69a132e9fd418219d27b8f9da2274f0dd4cf30a8c74603c6573dfd64c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>3D image cytometry</topic><topic>Algorithms</topic><topic>Animals</topic><topic>Fibroblasts - ultrastructure</topic><topic>Fluorescent Dyes</topic><topic>Image Cytometry - instrumentation</topic><topic>Image Cytometry - methods</topic><topic>Imaging, Three-Dimensional - instrumentation</topic><topic>Imaging, Three-Dimensional - methods</topic><topic>Kidney - ultrastructure</topic><topic>Lenses</topic><topic>Light</topic><topic>Lighting</topic><topic>Mice</topic><topic>Microscopy - instrumentation</topic><topic>Microscopy - methods</topic><topic>Muntjacs</topic><topic>rare cell detection</topic><topic>remote depth scanning</topic><topic>structured light illumination</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Heejin</creatorcontrib><creatorcontrib>Wadduwage, Dushan N.</creatorcontrib><creatorcontrib>Tu, Ting Yuan</creatorcontrib><creatorcontrib>Matsudaira, Paul</creatorcontrib><creatorcontrib>So, Peter T. 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C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three‐dimensional image cytometer based on widefield structured light microscopy and high‐speed remote depth scanning</atitle><jtitle>Cytometry. Part A</jtitle><addtitle>Cytometry A</addtitle><date>2015-01</date><risdate>2015</risdate><volume>87</volume><issue>1</issue><spage>49</spage><epage>60</epage><pages>49-60</pages><issn>1552-4922</issn><eissn>1552-4930</eissn><abstract>A high throughput 3D image cytometer have been developed that improves imaging speed by an order of magnitude over current technologies. This imaging speed improvement was realized by combining several key components. First, a depth‐resolved image can be rapidly generated using a structured light reconstruction algorithm that requires only two wide field images, one with uniform illumination and the other with structured illumination. Second, depth scanning is implemented using the high speed remote depth scanning. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3D image cytometry Algorithms Animals Fibroblasts - ultrastructure Fluorescent Dyes Image Cytometry - instrumentation Image Cytometry - methods Imaging, Three-Dimensional - instrumentation Imaging, Three-Dimensional - methods Kidney - ultrastructure Lenses Light Lighting Mice Microscopy - instrumentation Microscopy - methods Muntjacs rare cell detection remote depth scanning structured light illumination Time Factors |
| title | Three‐dimensional image cytometer based on widefield structured light microscopy and high‐speed remote depth scanning |
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