Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae
Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have y...
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| Veröffentlicht in: | BMC microbiology 2014-12, Vol.14 (1), p.317-317, Article 317 |
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| creator | Azad, Gajendra Kumar Singh, Vikash Thakare, Mayur Jankiram Baranwal, Shivani Tomar, Raghuvir Singh |
| description | Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described.
Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response.
Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae. |
| doi_str_mv | 10.1186/s12866-014-0317-0 |
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Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response.
Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae.</description><identifier>ISSN: 1471-2180</identifier><identifier>EISSN: 1471-2180</identifier><identifier>DOI: 10.1186/s12866-014-0317-0</identifier><identifier>PMID: 25523922</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Culture Media - chemistry ; Curcuma - chemistry ; Curcuma longa ; Curcumin - isolation & purification ; Curcumin - metabolism ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Enzymologic ; Glycerin ; Glycerol ; Iron - metabolism ; Kinases ; Mitogen-Activated Protein Kinases - metabolism ; Mitogens ; Phosphates ; Phosphorylation ; Physiological aspects ; Protein kinases ; Protein Processing, Post-Translational ; Proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae Proteins - metabolism ; Science education ; Studies ; Transcription factors ; Yeast</subject><ispartof>BMC microbiology, 2014-12, Vol.14 (1), p.317-317, Article 317</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>2014 Azad et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>Azad et al.; licensee BioMed Central. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b622t-731bf6c7b0ddfda07543a4d05d769f6f6d8df2263d8f5e35df0a1a782ab8f2fd3</citedby><cites>FETCH-LOGICAL-b622t-731bf6c7b0ddfda07543a4d05d769f6f6d8df2263d8f5e35df0a1a782ab8f2fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275933/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4275933/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25523922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azad, Gajendra Kumar</creatorcontrib><creatorcontrib>Singh, Vikash</creatorcontrib><creatorcontrib>Thakare, Mayur Jankiram</creatorcontrib><creatorcontrib>Baranwal, Shivani</creatorcontrib><creatorcontrib>Tomar, Raghuvir Singh</creatorcontrib><title>Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae</title><title>BMC microbiology</title><addtitle>BMC Microbiol</addtitle><description>Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described.
Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response.
Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae.</description><subject>Analysis</subject><subject>Culture Media - chemistry</subject><subject>Curcuma - chemistry</subject><subject>Curcuma longa</subject><subject>Curcumin - isolation & purification</subject><subject>Curcumin - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Glycerin</subject><subject>Glycerol</subject><subject>Iron - metabolism</subject><subject>Kinases</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Mitogens</subject><subject>Phosphates</subject><subject>Phosphorylation</subject><subject>Physiological aspects</subject><subject>Protein kinases</subject><subject>Protein Processing, Post-Translational</subject><subject>Proteins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Science education</subject><subject>Studies</subject><subject>Transcription factors</subject><subject>Yeast</subject><issn>1471-2180</issn><issn>1471-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkl1rFDEUhgdRbF39Ad7IgDd6MTUfk2T2Rij1o4WKYPU6ZJKT2dSdZJtklu6l_9wsW9uuVJBAEs77nJfDOaeqXmJ0hHHH3yVMOs4bhNsGUSwa9Kg6xK3ADcEdenzvf1A9S-kSISw6Kp5WB4QxQueEHFa_vrgcBvCN0tmtVQZTr2LI4Hz903mVoD4NA65dqu-AokVIq-CLmkOtp6insQThehXSFGEL5AXU_WSM80O9AZVyfaG0XqgYxo2GVGuIsHbJKXhePbFqmeDFzTurfnz6-P3ktDn_-vns5Pi86TkhuREU95Zr0SNjrFFIsJaq1iBmBJ9bbrnpjCWEU9NZBpQZixRWoiOq7yyxhs6q9zvf1dSPYDT4HNVSrqIbVdzIoJzcV7xbyCGsZUsEm1NaDD7sDHoX_mGwr-gwyt2IZBmR3I6oXLPqzU0dMVxNkLIcXdKwXCoPYUoSc8Ep7TD-H7QVCDNOREFf_4Vehin60tAthTAVlNA7alBLkM7bUArVW1N5zOiciZZxXKijB6hyDIxOBw_Wlfhewtu9hMJkuM6DmlKSZxff9lm8Y3UMKUWwtw3ESG63-sGWvbo_utuMP2tMfwMwPvPn</recordid><startdate>20141219</startdate><enddate>20141219</enddate><creator>Azad, Gajendra Kumar</creator><creator>Singh, Vikash</creator><creator>Thakare, Mayur Jankiram</creator><creator>Baranwal, Shivani</creator><creator>Tomar, Raghuvir Singh</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20141219</creationdate><title>Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae</title><author>Azad, Gajendra Kumar ; Singh, Vikash ; Thakare, Mayur Jankiram ; Baranwal, Shivani ; Tomar, Raghuvir Singh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b622t-731bf6c7b0ddfda07543a4d05d769f6f6d8df2263d8f5e35df0a1a782ab8f2fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Culture Media - chemistry</topic><topic>Curcuma - chemistry</topic><topic>Curcuma longa</topic><topic>Curcumin - isolation & purification</topic><topic>Curcumin - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Glycerin</topic><topic>Glycerol</topic><topic>Iron - metabolism</topic><topic>Kinases</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Mitogens</topic><topic>Phosphates</topic><topic>Phosphorylation</topic><topic>Physiological aspects</topic><topic>Protein kinases</topic><topic>Protein Processing, Post-Translational</topic><topic>Proteins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Science education</topic><topic>Studies</topic><topic>Transcription factors</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azad, Gajendra Kumar</creatorcontrib><creatorcontrib>Singh, Vikash</creatorcontrib><creatorcontrib>Thakare, Mayur Jankiram</creatorcontrib><creatorcontrib>Baranwal, Shivani</creatorcontrib><creatorcontrib>Tomar, Raghuvir Singh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azad, Gajendra Kumar</au><au>Singh, Vikash</au><au>Thakare, Mayur Jankiram</au><au>Baranwal, Shivani</au><au>Tomar, Raghuvir Singh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae</atitle><jtitle>BMC microbiology</jtitle><addtitle>BMC Microbiol</addtitle><date>2014-12-19</date><risdate>2014</risdate><volume>14</volume><issue>1</issue><spage>317</spage><epage>317</epage><pages>317-317</pages><artnum>317</artnum><issn>1471-2180</issn><eissn>1471-2180</eissn><abstract>Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described.
Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response.
Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25523922</pmid><doi>10.1186/s12866-014-0317-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Culture Media - chemistry Curcuma - chemistry Curcuma longa Curcumin - isolation & purification Curcumin - metabolism Gene expression Gene Expression Profiling Gene Expression Regulation, Enzymologic Glycerin Glycerol Iron - metabolism Kinases Mitogen-Activated Protein Kinases - metabolism Mitogens Phosphates Phosphorylation Physiological aspects Protein kinases Protein Processing, Post-Translational Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae Proteins - metabolism Science education Studies Transcription factors Yeast |
| title | Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae |
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