Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes

Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent‐molecule imaging. Surprisingly, in the intact PM, Bdp‐C...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Traffic (Copenhagen, Denmark) Denmark), 2014-06, Vol.15 (6), p.583-612
Hauptverfasser: Hiramoto‐Yamaki, Nao, Tanaka, Kenji A. K., Suzuki, Kenichi G. N., Hirosawa, Koichiro M., Miyahara, Manami S. H., Kalay, Ziya, Tanaka, Koichiro, Kasai, Rinshi S., Kusumi, Akihiro, Fujiwara, Takahiro K.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 612
container_issue 6
container_start_page 583
container_title Traffic (Copenhagen, Denmark)
container_volume 15
creator Hiramoto‐Yamaki, Nao
Tanaka, Kenji A. K.
Suzuki, Kenichi G. N.
Hirosawa, Koichiro M.
Miyahara, Manami S. H.
Kalay, Ziya
Tanaka, Koichiro
Kasai, Rinshi S.
Kusumi, Akihiro
Fujiwara, Takahiro K.
description Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent‐molecule imaging. Surprisingly, in the intact PM, Bdp‐Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non‐raft phospholipid molecules (0.33 µm2/second), despite Bdp‐Chol's probable association with raft domains. Furthermore, Bdp‐Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp‐Chol and Cy3‐conjugated dioleoylphosphatidylethanolamine (Cy3‐DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin‐based membrane skeleton reduces the D of Bdp‐Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3‐DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin‐based membrane‐skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp‐Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3‐DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them. A fluorescent cholesterol analogue, Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol), exhibited diffusion at the fastest rate ever found for any membrane molecules in the plasma membrane, with a median diffusion coefficient of 3.4 µm2/second, ∼10× greater than that of phospholipids, despite Bdp‐Chol's probable association with raft domains. Bdp‐Chol and phospholipids diffused at comparable rates in the absence of actin membrane‐skeleton, indicating that Bdp‐Chol hops across the actin‐fence‐pickets' compartment boundaries ∼10× faster than phospholipids. Raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them.
doi_str_mv 10.1111/tra.12163
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4265843</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1523406233</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5093-daef5b71852bbb0ed94a53967a227acf56ba84ed942a20bde2dc21554f652a4b3</originalsourceid><addsrcrecordid>eNp1kU9rFTEUxYMotlYXfgEJuLGLaZObZP5shMezVaGiSLuUcDOTtCmZyWsyo9RPb15fLSqYTcLNL4dzcgh5ydkRL-t4TnjEgdfiEdnnNWMVa5V8XM6ia6sOeLdHnuV8zRgDJeVTsgdSsVpAu0--XYTy2mGe6Tvv3JJ9nGh0FOlpWGKyubfTTNdXMdg82xQDXU0Y4iX1E13HcYNpHguBwf-0A_0SMI9IP9nRJJxsfk6eOAzZvrjfD8jF6cn5-kN19vn9x_XqrOoV60Q1oHXKNLxVYIxhdugkKtHVDQI02DtVG2zldgwIzAwWhh64UtLVClAacUDe7nQ3ixntsPWcMOhN8iOmWx3R679vJn-lL-N3LaFWrRRF4M29QIo3S4mqR1-ih1BSxCVrrkBIVoPYoq__Qa_jksqn3FHQcNnyplCHO6pPMedk3YMZzvS2NF186LvSCvvqT_cP5O-WCnC8A374YG__r6TPv652kr8Az_SilQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1522714817</pqid></control><display><type>article</type><title>Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes</title><source>IngentaConnect Backfiles</source><source>MEDLINE</source><source>Wiley Free Archive</source><source>Wiley-Blackwell Full Collection</source><source>Elektronische Zeitschriftenbibliothek - Freely accessible e-journals</source><creator>Hiramoto‐Yamaki, Nao ; Tanaka, Kenji A. K. ; Suzuki, Kenichi G. N. ; Hirosawa, Koichiro M. ; Miyahara, Manami S. H. ; Kalay, Ziya ; Tanaka, Koichiro ; Kasai, Rinshi S. ; Kusumi, Akihiro ; Fujiwara, Takahiro K.</creator><creatorcontrib>Hiramoto‐Yamaki, Nao ; Tanaka, Kenji A. K. ; Suzuki, Kenichi G. N. ; Hirosawa, Koichiro M. ; Miyahara, Manami S. H. ; Kalay, Ziya ; Tanaka, Koichiro ; Kasai, Rinshi S. ; Kusumi, Akihiro ; Fujiwara, Takahiro K.</creatorcontrib><description>Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent‐molecule imaging. Surprisingly, in the intact PM, Bdp‐Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non‐raft phospholipid molecules (0.33 µm2/second), despite Bdp‐Chol's probable association with raft domains. Furthermore, Bdp‐Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp‐Chol and Cy3‐conjugated dioleoylphosphatidylethanolamine (Cy3‐DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin‐based membrane skeleton reduces the D of Bdp‐Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3‐DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin‐based membrane‐skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp‐Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3‐DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them. A fluorescent cholesterol analogue, Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol), exhibited diffusion at the fastest rate ever found for any membrane molecules in the plasma membrane, with a median diffusion coefficient of 3.4 µm2/second, ∼10× greater than that of phospholipids, despite Bdp‐Chol's probable association with raft domains. Bdp‐Chol and phospholipids diffused at comparable rates in the absence of actin membrane‐skeleton, indicating that Bdp‐Chol hops across the actin‐fence‐pickets' compartment boundaries ∼10× faster than phospholipids. Raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them.</description><identifier>ISSN: 1398-9219</identifier><identifier>EISSN: 1600-0854</identifier><identifier>DOI: 10.1111/tra.12163</identifier><identifier>PMID: 24506328</identifier><language>eng</language><publisher>Former Munksgaard: John Wiley &amp; Sons A/S</publisher><subject>Actin Cytoskeleton - metabolism ; Animals ; Boron Compounds ; Cell Line ; cholesterol ; Cholesterol - analogs &amp; derivatives ; Cholesterol - metabolism ; Diffusion ; Fluorescent Dyes ; hop diffusion ; Membrane Microdomains - drug effects ; Membrane Microdomains - metabolism ; Original ; Phosphatidylethanolamines - pharmacology ; phospholipids ; raft domains ; Rats ; single fluorescent‐molecule tracking ; single‐particle tracking</subject><ispartof>Traffic (Copenhagen, Denmark), 2014-06, Vol.15 (6), p.583-612</ispartof><rights>2014 The Authors. published by John Wiley &amp; Sons Ltd.</rights><rights>2014 John Wiley &amp; Sons A/S. Published by John Wiley &amp; Sons Ltd.</rights><rights>2014 John Wiley &amp; Sons A/S. Published by John Wiley &amp; Sons Ltd</rights><rights>2014 The Authors. published by John Wiley &amp; Sons Ltd 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5093-daef5b71852bbb0ed94a53967a227acf56ba84ed942a20bde2dc21554f652a4b3</citedby><cites>FETCH-LOGICAL-c5093-daef5b71852bbb0ed94a53967a227acf56ba84ed942a20bde2dc21554f652a4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftra.12163$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftra.12163$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24506328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiramoto‐Yamaki, Nao</creatorcontrib><creatorcontrib>Tanaka, Kenji A. K.</creatorcontrib><creatorcontrib>Suzuki, Kenichi G. N.</creatorcontrib><creatorcontrib>Hirosawa, Koichiro M.</creatorcontrib><creatorcontrib>Miyahara, Manami S. H.</creatorcontrib><creatorcontrib>Kalay, Ziya</creatorcontrib><creatorcontrib>Tanaka, Koichiro</creatorcontrib><creatorcontrib>Kasai, Rinshi S.</creatorcontrib><creatorcontrib>Kusumi, Akihiro</creatorcontrib><creatorcontrib>Fujiwara, Takahiro K.</creatorcontrib><title>Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes</title><title>Traffic (Copenhagen, Denmark)</title><addtitle>Traffic</addtitle><description>Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent‐molecule imaging. Surprisingly, in the intact PM, Bdp‐Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non‐raft phospholipid molecules (0.33 µm2/second), despite Bdp‐Chol's probable association with raft domains. Furthermore, Bdp‐Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp‐Chol and Cy3‐conjugated dioleoylphosphatidylethanolamine (Cy3‐DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin‐based membrane skeleton reduces the D of Bdp‐Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3‐DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin‐based membrane‐skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp‐Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3‐DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them. A fluorescent cholesterol analogue, Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol), exhibited diffusion at the fastest rate ever found for any membrane molecules in the plasma membrane, with a median diffusion coefficient of 3.4 µm2/second, ∼10× greater than that of phospholipids, despite Bdp‐Chol's probable association with raft domains. Bdp‐Chol and phospholipids diffused at comparable rates in the absence of actin membrane‐skeleton, indicating that Bdp‐Chol hops across the actin‐fence‐pickets' compartment boundaries ∼10× faster than phospholipids. Raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them.</description><subject>Actin Cytoskeleton - metabolism</subject><subject>Animals</subject><subject>Boron Compounds</subject><subject>Cell Line</subject><subject>cholesterol</subject><subject>Cholesterol - analogs &amp; derivatives</subject><subject>Cholesterol - metabolism</subject><subject>Diffusion</subject><subject>Fluorescent Dyes</subject><subject>hop diffusion</subject><subject>Membrane Microdomains - drug effects</subject><subject>Membrane Microdomains - metabolism</subject><subject>Original</subject><subject>Phosphatidylethanolamines - pharmacology</subject><subject>phospholipids</subject><subject>raft domains</subject><subject>Rats</subject><subject>single fluorescent‐molecule tracking</subject><subject>single‐particle tracking</subject><issn>1398-9219</issn><issn>1600-0854</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNp1kU9rFTEUxYMotlYXfgEJuLGLaZObZP5shMezVaGiSLuUcDOTtCmZyWsyo9RPb15fLSqYTcLNL4dzcgh5ydkRL-t4TnjEgdfiEdnnNWMVa5V8XM6ia6sOeLdHnuV8zRgDJeVTsgdSsVpAu0--XYTy2mGe6Tvv3JJ9nGh0FOlpWGKyubfTTNdXMdg82xQDXU0Y4iX1E13HcYNpHguBwf-0A_0SMI9IP9nRJJxsfk6eOAzZvrjfD8jF6cn5-kN19vn9x_XqrOoV60Q1oHXKNLxVYIxhdugkKtHVDQI02DtVG2zldgwIzAwWhh64UtLVClAacUDe7nQ3ixntsPWcMOhN8iOmWx3R679vJn-lL-N3LaFWrRRF4M29QIo3S4mqR1-ih1BSxCVrrkBIVoPYoq__Qa_jksqn3FHQcNnyplCHO6pPMedk3YMZzvS2NF186LvSCvvqT_cP5O-WCnC8A374YG__r6TPv652kr8Az_SilQ</recordid><startdate>201406</startdate><enddate>201406</enddate><creator>Hiramoto‐Yamaki, Nao</creator><creator>Tanaka, Kenji A. K.</creator><creator>Suzuki, Kenichi G. N.</creator><creator>Hirosawa, Koichiro M.</creator><creator>Miyahara, Manami S. H.</creator><creator>Kalay, Ziya</creator><creator>Tanaka, Koichiro</creator><creator>Kasai, Rinshi S.</creator><creator>Kusumi, Akihiro</creator><creator>Fujiwara, Takahiro K.</creator><general>John Wiley &amp; Sons A/S</general><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201406</creationdate><title>Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes</title><author>Hiramoto‐Yamaki, Nao ; Tanaka, Kenji A. K. ; Suzuki, Kenichi G. N. ; Hirosawa, Koichiro M. ; Miyahara, Manami S. H. ; Kalay, Ziya ; Tanaka, Koichiro ; Kasai, Rinshi S. ; Kusumi, Akihiro ; Fujiwara, Takahiro K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5093-daef5b71852bbb0ed94a53967a227acf56ba84ed942a20bde2dc21554f652a4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Actin Cytoskeleton - metabolism</topic><topic>Animals</topic><topic>Boron Compounds</topic><topic>Cell Line</topic><topic>cholesterol</topic><topic>Cholesterol - analogs &amp; derivatives</topic><topic>Cholesterol - metabolism</topic><topic>Diffusion</topic><topic>Fluorescent Dyes</topic><topic>hop diffusion</topic><topic>Membrane Microdomains - drug effects</topic><topic>Membrane Microdomains - metabolism</topic><topic>Original</topic><topic>Phosphatidylethanolamines - pharmacology</topic><topic>phospholipids</topic><topic>raft domains</topic><topic>Rats</topic><topic>single fluorescent‐molecule tracking</topic><topic>single‐particle tracking</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiramoto‐Yamaki, Nao</creatorcontrib><creatorcontrib>Tanaka, Kenji A. K.</creatorcontrib><creatorcontrib>Suzuki, Kenichi G. N.</creatorcontrib><creatorcontrib>Hirosawa, Koichiro M.</creatorcontrib><creatorcontrib>Miyahara, Manami S. H.</creatorcontrib><creatorcontrib>Kalay, Ziya</creatorcontrib><creatorcontrib>Tanaka, Koichiro</creatorcontrib><creatorcontrib>Kasai, Rinshi S.</creatorcontrib><creatorcontrib>Kusumi, Akihiro</creatorcontrib><creatorcontrib>Fujiwara, Takahiro K.</creatorcontrib><collection>Wiley-Blackwell Open Access Collection</collection><collection>Wiley Free Archive</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Traffic (Copenhagen, Denmark)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiramoto‐Yamaki, Nao</au><au>Tanaka, Kenji A. K.</au><au>Suzuki, Kenichi G. N.</au><au>Hirosawa, Koichiro M.</au><au>Miyahara, Manami S. H.</au><au>Kalay, Ziya</au><au>Tanaka, Koichiro</au><au>Kasai, Rinshi S.</au><au>Kusumi, Akihiro</au><au>Fujiwara, Takahiro K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes</atitle><jtitle>Traffic (Copenhagen, Denmark)</jtitle><addtitle>Traffic</addtitle><date>2014-06</date><risdate>2014</risdate><volume>15</volume><issue>6</issue><spage>583</spage><epage>612</epage><pages>583-612</pages><issn>1398-9219</issn><eissn>1600-0854</eissn><abstract>Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent‐molecule imaging. Surprisingly, in the intact PM, Bdp‐Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non‐raft phospholipid molecules (0.33 µm2/second), despite Bdp‐Chol's probable association with raft domains. Furthermore, Bdp‐Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp‐Chol and Cy3‐conjugated dioleoylphosphatidylethanolamine (Cy3‐DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin‐based membrane skeleton reduces the D of Bdp‐Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3‐DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin‐based membrane‐skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp‐Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3‐DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them. A fluorescent cholesterol analogue, Bodipy488‐conjugated cholesterol molecule (Bdp‐Chol), exhibited diffusion at the fastest rate ever found for any membrane molecules in the plasma membrane, with a median diffusion coefficient of 3.4 µm2/second, ∼10× greater than that of phospholipids, despite Bdp‐Chol's probable association with raft domains. Bdp‐Chol and phospholipids diffused at comparable rates in the absence of actin membrane‐skeleton, indicating that Bdp‐Chol hops across the actin‐fence‐pickets' compartment boundaries ∼10× faster than phospholipids. Raft domains coexist with membrane‐skeleton‐induced compartments and are contained within them.</abstract><cop>Former Munksgaard</cop><pub>John Wiley &amp; Sons A/S</pub><pmid>24506328</pmid><doi>10.1111/tra.12163</doi><tpages>30</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1398-9219
ispartof Traffic (Copenhagen, Denmark), 2014-06, Vol.15 (6), p.583-612
issn 1398-9219
1600-0854
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4265843
source IngentaConnect Backfiles; MEDLINE; Wiley Free Archive; Wiley-Blackwell Full Collection; Elektronische Zeitschriftenbibliothek - Freely accessible e-journals
subjects Actin Cytoskeleton - metabolism
Animals
Boron Compounds
Cell Line
cholesterol
Cholesterol - analogs & derivatives
Cholesterol - metabolism
Diffusion
Fluorescent Dyes
hop diffusion
Membrane Microdomains - drug effects
Membrane Microdomains - metabolism
Original
Phosphatidylethanolamines - pharmacology
phospholipids
raft domains
Rats
single fluorescent‐molecule tracking
single‐particle tracking
title Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T19%3A48%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultrafast%20Diffusion%20of%20a%20Fluorescent%20Cholesterol%20Analog%20in%20Compartmentalized%20Plasma%20Membranes&rft.jtitle=Traffic%20(Copenhagen,%20Denmark)&rft.au=Hiramoto%E2%80%90Yamaki,%20Nao&rft.date=2014-06&rft.volume=15&rft.issue=6&rft.spage=583&rft.epage=612&rft.pages=583-612&rft.issn=1398-9219&rft.eissn=1600-0854&rft_id=info:doi/10.1111/tra.12163&rft_dat=%3Cproquest_pubme%3E1523406233%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1522714817&rft_id=info:pmid/24506328&rfr_iscdi=true