Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi
In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using...
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| Veröffentlicht in: | Scientific reports 2014-12, Vol.4 (1), p.7435, Article 7435 |
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| creator | Epis, Sara Porretta, Daniele Mastrantonio, Valentina Urbanelli, Sandra Sassera, Davide De Marco, Leone Mereghetti, Valeria Montagna, Matteo Ricci, Irene Favia, Guido Bandi, Claudio |
| description | In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector
Anopheles stephensi
as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps. |
| doi_str_mv | 10.1038/srep07435 |
| format | Article |
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Anopheles stephensi
as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep07435</identifier><identifier>PMID: 25504146</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>38 ; 38/39 ; 38/77 ; 38/90 ; 631/158/2464 ; 704/158/2452 ; ABC transporter ; ABC transporters ; Amino Acid Sequence ; Animals ; Anopheles - drug effects ; Anopheles - genetics ; Anopheles - metabolism ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Female ; Gene expression ; Gene Expression - drug effects ; Gene Expression Regulation - drug effects ; Humanities and Social Sciences ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insect Vectors - drug effects ; Insect Vectors - genetics ; Insect Vectors - metabolism ; Insecticides ; Insecticides - pharmacology ; Investigations ; Larva - drug effects ; Larva - genetics ; Larva - metabolism ; Larvae ; Malaria ; Molecular Sequence Data ; multidisciplinary ; Permethrin ; Permethrin - pharmacology ; Science ; Sequence Homology, Amino Acid ; Vector-borne diseases</subject><ispartof>Scientific reports, 2014-12, Vol.4 (1), p.7435, Article 7435</ispartof><rights>The Author(s) 2014</rights><rights>Copyright Nature Publishing Group Dec 2014</rights><rights>Copyright © 2014, Macmillan Publishers Limited. All rights reserved 2014 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-41bf1e713e21bd03a5e161a7742a3824ddbd1cde18de52683217c31e022b7e8e3</citedby><cites>FETCH-LOGICAL-c438t-41bf1e713e21bd03a5e161a7742a3824ddbd1cde18de52683217c31e022b7e8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262823/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262823/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,41096,42165,51551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25504146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Epis, Sara</creatorcontrib><creatorcontrib>Porretta, Daniele</creatorcontrib><creatorcontrib>Mastrantonio, Valentina</creatorcontrib><creatorcontrib>Urbanelli, Sandra</creatorcontrib><creatorcontrib>Sassera, Davide</creatorcontrib><creatorcontrib>De Marco, Leone</creatorcontrib><creatorcontrib>Mereghetti, Valeria</creatorcontrib><creatorcontrib>Montagna, Matteo</creatorcontrib><creatorcontrib>Ricci, Irene</creatorcontrib><creatorcontrib>Favia, Guido</creatorcontrib><creatorcontrib>Bandi, Claudio</creatorcontrib><title>Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector
Anopheles stephensi
as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.</description><subject>38</subject><subject>38/39</subject><subject>38/77</subject><subject>38/90</subject><subject>631/158/2464</subject><subject>704/158/2452</subject><subject>ABC transporter</subject><subject>ABC transporters</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Anopheles - drug effects</subject><subject>Anopheles - genetics</subject><subject>Anopheles - metabolism</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Humanities and Social Sciences</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insect Vectors - drug effects</subject><subject>Insect Vectors - genetics</subject><subject>Insect Vectors - metabolism</subject><subject>Insecticides</subject><subject>Insecticides - pharmacology</subject><subject>Investigations</subject><subject>Larva - drug effects</subject><subject>Larva - genetics</subject><subject>Larva - metabolism</subject><subject>Larvae</subject><subject>Malaria</subject><subject>Molecular Sequence Data</subject><subject>multidisciplinary</subject><subject>Permethrin</subject><subject>Permethrin - pharmacology</subject><subject>Science</subject><subject>Sequence Homology, Amino Acid</subject><subject>Vector-borne 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reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Epis, Sara</au><au>Porretta, Daniele</au><au>Mastrantonio, Valentina</au><au>Urbanelli, Sandra</au><au>Sassera, Davide</au><au>De Marco, Leone</au><au>Mereghetti, Valeria</au><au>Montagna, Matteo</au><au>Ricci, Irene</au><au>Favia, Guido</au><au>Bandi, Claudio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-12-11</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>7435</spage><pages>7435-</pages><artnum>7435</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In insects, ABC transporters have been shown to contribute to defence/resistance to insecticides by reducing toxic concentrations in cells/tissues. Despite the extensive studies about this detoxifying mechanism, the temporal patterns of ABC transporter activation have been poorly investigated. Using the malaria vector
Anopheles stephensi
as a study system, we investigated the expression profile of ABC genes belonging to different subfamilies in permethrin-treated larvae at different time points (30 min to 48 h). Our results showed that the expression of ABCB and ABCG subfamily genes was upregulated at 1 h after treatment, with the highest expression observed at 6 h. Therefore, future investigations on the temporal dynamics of ABC gene expression will allow a better implementation of insecticide treatment regimens, including the use of specific inhibitors of ABC efflux pumps.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>25504146</pmid><doi>10.1038/srep07435</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 38 38/39 38/77 38/90 631/158/2464 704/158/2452 ABC transporter ABC transporters Amino Acid Sequence Animals Anopheles - drug effects Anopheles - genetics Anopheles - metabolism ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Female Gene expression Gene Expression - drug effects Gene Expression Regulation - drug effects Humanities and Social Sciences Insect Proteins - genetics Insect Proteins - metabolism Insect Vectors - drug effects Insect Vectors - genetics Insect Vectors - metabolism Insecticides Insecticides - pharmacology Investigations Larva - drug effects Larva - genetics Larva - metabolism Larvae Malaria Molecular Sequence Data multidisciplinary Permethrin Permethrin - pharmacology Science Sequence Homology, Amino Acid Vector-borne diseases |
| title | Temporal dynamics of the ABC transporter response to insecticide treatment: insights from the malaria vector Anopheles stephensi |
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