Evaluation of Intraspecies Interactions in Biofilm Formation by Methylobacterium Species Isolated from Pink-Pigmented Household Biofilms
Concern regarding household biofilms has grown due to their widespread existence and potential to threaten human health by serving as pathogen reservoirs. Previous studies identified Methylobacterium as one of the dominant genera found in household biofilms. In the present study, we examined the mec...
Gespeichert in:
| Veröffentlicht in: | Microbes and Environments 2014, Vol.29(4), pp.388-392 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 392 |
|---|---|
| container_issue | 4 |
| container_start_page | 388 |
| container_title | Microbes and Environments |
| container_volume | 29 |
| creator | Xu, Fang-Fang Morohoshi, Tomohiro Wang, Wen-Zhao Yamaguchi, Yuka Liang, Yan Ikeda, Tsukasa |
| description | Concern regarding household biofilms has grown due to their widespread existence and potential to threaten human health by serving as pathogen reservoirs. Previous studies identified Methylobacterium as one of the dominant genera found in household biofilms. In the present study, we examined the mechanisms underlying biofilm formation by using the bacterial consortium found in household pink slime. A clone library analysis revealed that Methylobacterium was the predominant genus in household pink slime. In addition, 16 out of 21 pink-pigmented bacterial isolates were assigned to the genus Methylobacterium. Although all of the Methylobacterium isolates formed low-level biofilms, the amount of the biofilms formed by Methylobacterium sp. P-1M and P-18S was significantly increased by co-culturing with other Methylobacterium strains that belonged to a specific phylogenetic group. The single-species biofilm was easily washed from the glass surface, whereas the dual-species biofilm strongly adhered after washing. A confocal laser scanning microscopy analysis showed that the dual-species biofilms were significantly thicker and tighter than the single-species biofilms. |
| doi_str_mv | 10.1264/jsme2.ME14038 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4262362</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3919713671</sourcerecordid><originalsourceid>FETCH-LOGICAL-c658t-f9e56da053d953cb058ffe455ee8086b4cbe3754df1d5471e728c742ca3b6ea03</originalsourceid><addsrcrecordid>eNpdkV1r2zAUhsXYaLusl7sdgt3sxp2-7dwM1pCuhZYV1l4LWT5OlMlWJtmF_IP97NmJE7aB0Nd5ziu9vAi9p-SKMiU-b1ID7OphSQXhxSt0QbnIMyGIfL3fs0xxSs_R25Q2hHAuc3aGzpnkBc2pvEC_ly_G96ZzocWhxndtF03agnWQxgNEY8dawq7F1y7Uzjf4JsTm0FHu8AN0650P5cBBdH2Dfxy7U_CmgwrXMTT40bU_s0e3aqAd725Dn2AdfHUUTe_Qm9r4BJfTOkPPN8unxW12__3b3eLrfWaVLLqsnoNUlSGSV3PJbUlkUdcgpAQoSKFKYUvguRRVTSspcgo5K2wumDW8VGAIn6EvB91tXzZQWRgde72NrjFxp4Nx-t9K69Z6FV60YIrxYczQp0kghl89pE43Llnw3rQwuNI0V3IuWMHHtz7-h25CH9vB3kBJxVhB91R2oGwMKUWoT5-hRI8Z633Gesp44D_87eBEH0MdgMUB2KTOrOAEmNg562GSY3MtxmmSPVXt2kQNLf8D3ni-vQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1756228130</pqid></control><display><type>article</type><title>Evaluation of Intraspecies Interactions in Biofilm Formation by Methylobacterium Species Isolated from Pink-Pigmented Household Biofilms</title><source>J-STAGE Free</source><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Xu, Fang-Fang ; Morohoshi, Tomohiro ; Wang, Wen-Zhao ; Yamaguchi, Yuka ; Liang, Yan ; Ikeda, Tsukasa</creator><creatorcontrib>Xu, Fang-Fang ; Morohoshi, Tomohiro ; Wang, Wen-Zhao ; Yamaguchi, Yuka ; Liang, Yan ; Ikeda, Tsukasa</creatorcontrib><description>Concern regarding household biofilms has grown due to their widespread existence and potential to threaten human health by serving as pathogen reservoirs. Previous studies identified Methylobacterium as one of the dominant genera found in household biofilms. In the present study, we examined the mechanisms underlying biofilm formation by using the bacterial consortium found in household pink slime. A clone library analysis revealed that Methylobacterium was the predominant genus in household pink slime. In addition, 16 out of 21 pink-pigmented bacterial isolates were assigned to the genus Methylobacterium. Although all of the Methylobacterium isolates formed low-level biofilms, the amount of the biofilms formed by Methylobacterium sp. P-1M and P-18S was significantly increased by co-culturing with other Methylobacterium strains that belonged to a specific phylogenetic group. The single-species biofilm was easily washed from the glass surface, whereas the dual-species biofilm strongly adhered after washing. A confocal laser scanning microscopy analysis showed that the dual-species biofilms were significantly thicker and tighter than the single-species biofilms.</description><identifier>ISSN: 1342-6311</identifier><identifier>EISSN: 1347-4405</identifier><identifier>DOI: 10.1264/jsme2.ME14038</identifier><identifier>PMID: 25381715</identifier><language>eng</language><publisher>Japan: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</publisher><subject>Biofilms - growth & development ; Cluster Analysis ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Environmental Microbiology ; Family Characteristics ; household biofilm ; Humans ; intraspecies interaction ; Methylobacterium ; Methylobacterium - classification ; Methylobacterium - growth & development ; Methylobacterium - isolation & purification ; Methylobacterium - physiology ; Microbial Interactions ; Microscopy, Confocal ; Molecular Sequence Data ; Phylogeny ; Pigments, Biological - analysis ; pink biofilm ; RNA, Ribosomal, 18S - genetics ; Sequence Analysis, DNA</subject><ispartof>Microbes and Environments, 2014, Vol.29(4), pp.388-392</ispartof><rights>2014 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology</rights><rights>Copyright Japan Science and Technology Agency 2014</rights><rights>Copyright 2014 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology 2014</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c658t-f9e56da053d953cb058ffe455ee8086b4cbe3754df1d5471e728c742ca3b6ea03</citedby><cites>FETCH-LOGICAL-c658t-f9e56da053d953cb058ffe455ee8086b4cbe3754df1d5471e728c742ca3b6ea03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262362/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4262362/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,1877,4010,27900,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25381715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Fang-Fang</creatorcontrib><creatorcontrib>Morohoshi, Tomohiro</creatorcontrib><creatorcontrib>Wang, Wen-Zhao</creatorcontrib><creatorcontrib>Yamaguchi, Yuka</creatorcontrib><creatorcontrib>Liang, Yan</creatorcontrib><creatorcontrib>Ikeda, Tsukasa</creatorcontrib><title>Evaluation of Intraspecies Interactions in Biofilm Formation by Methylobacterium Species Isolated from Pink-Pigmented Household Biofilms</title><title>Microbes and Environments</title><addtitle>Microbes Environ.</addtitle><description>Concern regarding household biofilms has grown due to their widespread existence and potential to threaten human health by serving as pathogen reservoirs. Previous studies identified Methylobacterium as one of the dominant genera found in household biofilms. In the present study, we examined the mechanisms underlying biofilm formation by using the bacterial consortium found in household pink slime. A clone library analysis revealed that Methylobacterium was the predominant genus in household pink slime. In addition, 16 out of 21 pink-pigmented bacterial isolates were assigned to the genus Methylobacterium. Although all of the Methylobacterium isolates formed low-level biofilms, the amount of the biofilms formed by Methylobacterium sp. P-1M and P-18S was significantly increased by co-culturing with other Methylobacterium strains that belonged to a specific phylogenetic group. The single-species biofilm was easily washed from the glass surface, whereas the dual-species biofilm strongly adhered after washing. A confocal laser scanning microscopy analysis showed that the dual-species biofilms were significantly thicker and tighter than the single-species biofilms.</description><subject>Biofilms - growth & development</subject><subject>Cluster Analysis</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Environmental Microbiology</subject><subject>Family Characteristics</subject><subject>household biofilm</subject><subject>Humans</subject><subject>intraspecies interaction</subject><subject>Methylobacterium</subject><subject>Methylobacterium - classification</subject><subject>Methylobacterium - growth & development</subject><subject>Methylobacterium - isolation & purification</subject><subject>Methylobacterium - physiology</subject><subject>Microbial Interactions</subject><subject>Microscopy, Confocal</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Pigments, Biological - analysis</subject><subject>pink biofilm</subject><subject>RNA, Ribosomal, 18S - genetics</subject><subject>Sequence Analysis, DNA</subject><issn>1342-6311</issn><issn>1347-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkV1r2zAUhsXYaLusl7sdgt3sxp2-7dwM1pCuhZYV1l4LWT5OlMlWJtmF_IP97NmJE7aB0Nd5ziu9vAi9p-SKMiU-b1ID7OphSQXhxSt0QbnIMyGIfL3fs0xxSs_R25Q2hHAuc3aGzpnkBc2pvEC_ly_G96ZzocWhxndtF03agnWQxgNEY8dawq7F1y7Uzjf4JsTm0FHu8AN0650P5cBBdH2Dfxy7U_CmgwrXMTT40bU_s0e3aqAd725Dn2AdfHUUTe_Qm9r4BJfTOkPPN8unxW12__3b3eLrfWaVLLqsnoNUlSGSV3PJbUlkUdcgpAQoSKFKYUvguRRVTSspcgo5K2wumDW8VGAIn6EvB91tXzZQWRgde72NrjFxp4Nx-t9K69Z6FV60YIrxYczQp0kghl89pE43Llnw3rQwuNI0V3IuWMHHtz7-h25CH9vB3kBJxVhB91R2oGwMKUWoT5-hRI8Z633Gesp44D_87eBEH0MdgMUB2KTOrOAEmNg562GSY3MtxmmSPVXt2kQNLf8D3ni-vQ</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Xu, Fang-Fang</creator><creator>Morohoshi, Tomohiro</creator><creator>Wang, Wen-Zhao</creator><creator>Yamaguchi, Yuka</creator><creator>Liang, Yan</creator><creator>Ikeda, Tsukasa</creator><general>Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</general><general>Japan Science and Technology Agency</general><general>The Japanese Society of Microbial Ecology (JSME)/The Japanese Society of Soil Microbiology (JSSM)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>5PM</scope></search><sort><creationdate>2014</creationdate><title>Evaluation of Intraspecies Interactions in Biofilm Formation by Methylobacterium Species Isolated from Pink-Pigmented Household Biofilms</title><author>Xu, Fang-Fang ; Morohoshi, Tomohiro ; Wang, Wen-Zhao ; Yamaguchi, Yuka ; Liang, Yan ; Ikeda, Tsukasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c658t-f9e56da053d953cb058ffe455ee8086b4cbe3754df1d5471e728c742ca3b6ea03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biofilms - growth & development</topic><topic>Cluster Analysis</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Environmental Microbiology</topic><topic>Family Characteristics</topic><topic>household biofilm</topic><topic>Humans</topic><topic>intraspecies interaction</topic><topic>Methylobacterium</topic><topic>Methylobacterium - classification</topic><topic>Methylobacterium - growth & development</topic><topic>Methylobacterium - isolation & purification</topic><topic>Methylobacterium - physiology</topic><topic>Microbial Interactions</topic><topic>Microscopy, Confocal</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Pigments, Biological - analysis</topic><topic>pink biofilm</topic><topic>RNA, Ribosomal, 18S - genetics</topic><topic>Sequence Analysis, DNA</topic><toplevel>online_resources</toplevel><creatorcontrib>Xu, Fang-Fang</creatorcontrib><creatorcontrib>Morohoshi, Tomohiro</creatorcontrib><creatorcontrib>Wang, Wen-Zhao</creatorcontrib><creatorcontrib>Yamaguchi, Yuka</creatorcontrib><creatorcontrib>Liang, Yan</creatorcontrib><creatorcontrib>Ikeda, Tsukasa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbes and Environments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Fang-Fang</au><au>Morohoshi, Tomohiro</au><au>Wang, Wen-Zhao</au><au>Yamaguchi, Yuka</au><au>Liang, Yan</au><au>Ikeda, Tsukasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Intraspecies Interactions in Biofilm Formation by Methylobacterium Species Isolated from Pink-Pigmented Household Biofilms</atitle><jtitle>Microbes and Environments</jtitle><addtitle>Microbes Environ.</addtitle><date>2014</date><risdate>2014</risdate><volume>29</volume><issue>4</issue><spage>388</spage><epage>392</epage><pages>388-392</pages><issn>1342-6311</issn><eissn>1347-4405</eissn><abstract>Concern regarding household biofilms has grown due to their widespread existence and potential to threaten human health by serving as pathogen reservoirs. Previous studies identified Methylobacterium as one of the dominant genera found in household biofilms. In the present study, we examined the mechanisms underlying biofilm formation by using the bacterial consortium found in household pink slime. A clone library analysis revealed that Methylobacterium was the predominant genus in household pink slime. In addition, 16 out of 21 pink-pigmented bacterial isolates were assigned to the genus Methylobacterium. Although all of the Methylobacterium isolates formed low-level biofilms, the amount of the biofilms formed by Methylobacterium sp. P-1M and P-18S was significantly increased by co-culturing with other Methylobacterium strains that belonged to a specific phylogenetic group. The single-species biofilm was easily washed from the glass surface, whereas the dual-species biofilm strongly adhered after washing. A confocal laser scanning microscopy analysis showed that the dual-species biofilms were significantly thicker and tighter than the single-species biofilms.</abstract><cop>Japan</cop><pub>Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles</pub><pmid>25381715</pmid><doi>10.1264/jsme2.ME14038</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1342-6311 |
| ispartof | Microbes and Environments, 2014, Vol.29(4), pp.388-392 |
| issn | 1342-6311 1347-4405 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4262362 |
| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Biofilms - growth & development Cluster Analysis DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Environmental Microbiology Family Characteristics household biofilm Humans intraspecies interaction Methylobacterium Methylobacterium - classification Methylobacterium - growth & development Methylobacterium - isolation & purification Methylobacterium - physiology Microbial Interactions Microscopy, Confocal Molecular Sequence Data Phylogeny Pigments, Biological - analysis pink biofilm RNA, Ribosomal, 18S - genetics Sequence Analysis, DNA |
| title | Evaluation of Intraspecies Interactions in Biofilm Formation by Methylobacterium Species Isolated from Pink-Pigmented Household Biofilms |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T05%3A56%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20Intraspecies%20Interactions%20in%20Biofilm%20Formation%20by%20Methylobacterium%20Species%20Isolated%20from%20Pink-Pigmented%20Household%20Biofilms&rft.jtitle=Microbes%20and%20Environments&rft.au=Xu,%20Fang-Fang&rft.date=2014&rft.volume=29&rft.issue=4&rft.spage=388&rft.epage=392&rft.pages=388-392&rft.issn=1342-6311&rft.eissn=1347-4405&rft_id=info:doi/10.1264/jsme2.ME14038&rft_dat=%3Cproquest_pubme%3E3919713671%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1756228130&rft_id=info:pmid/25381715&rfr_iscdi=true |