Analysis of methylation and mRNA expression status ofFADD andFAS genes in patients with oral squamous cell carcinoma
Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methyla...
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| Veröffentlicht in: | Medicina oral, patología oral y cirugía bucal patología oral y cirugía bucal, 2014-08, Vol.19 (6), p.e562-e568 |
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| container_title | Medicina oral, patología oral y cirugía bucal |
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| creator | Saberi, Eshaghali Kordi-Tamandani, Dor M. Jamali, Sara Rigi-Ladez, Mohammad A. Augend, Arsalan |
| description | Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of
FADD
and
FAS
genes in risk of OSCC.
Material and Methods: we analyze the promoter methylation status of
FADD
and
FAS
genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also,
FADD
and
FAS
genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR.
Results: Aberrant promoter methylation of
FADD
and
FAS
genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both
FADD
and
FAS
genes (
P |
| doi_str_mv | 10.4317/medoral.19805 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmedcentral</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4259371</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_4259371</sourcerecordid><originalsourceid>FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_42593713</originalsourceid><addsrcrecordid>eNqljztPw0AQhE8IRMKjpN8_kOCzz3bcIFkEKxUF0J8W5xIfuofxngH_e85SGmqqXe3ON5ph7I4na5Hx8t6qvR_QrHm1SfIztuRFtVkVlSjOT7sQolywK6KPJMlKXhaXbJHmPK1SkS9ZqB2aiTSBP4BVoZsMBu0doNuDfXmuQf30gyKabxQwjLOyqbfbWdHUr3BUThFoB30ElQsE3zp0MIcC-hzR-oi0yhhocWi18xZv2MUBDanb07xmD83T2-Nu1Y_vsU8bXSIt-0FbHCbpUcu_H6c7efRfUqR5FTtl_zb4BU5GbAo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Analysis of methylation and mRNA expression status ofFADD andFAS genes in patients with oral squamous cell carcinoma</title><source>PubMed Central Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Saberi, Eshaghali ; Kordi-Tamandani, Dor M. ; Jamali, Sara ; Rigi-Ladez, Mohammad A. ; Augend, Arsalan</creator><creatorcontrib>Saberi, Eshaghali ; Kordi-Tamandani, Dor M. ; Jamali, Sara ; Rigi-Ladez, Mohammad A. ; Augend, Arsalan</creatorcontrib><description>Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of
FADD
and
FAS
genes in risk of OSCC.
Material and Methods: we analyze the promoter methylation status of
FADD
and
FAS
genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also,
FADD
and
FAS
genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR.
Results: Aberrant promoter methylation of
FADD
and
FAS
genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both
FADD
and
FAS
genes (
P
<0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both
FADD
(
p
<0.02) and
FAS
(
p
<0.007) genes.
Conclusions: To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the
FADD
and
FAS
genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations.
Key words:
OSCC, FADD, FAS, DNA methylation, gene expression.</description><identifier>ISSN: 1698-4447</identifier><identifier>EISSN: 1698-6946</identifier><identifier>DOI: 10.4317/medoral.19805</identifier><identifier>PMID: 25129245</identifier><language>eng</language><publisher>Medicina Oral S.L</publisher><ispartof>Medicina oral, patología oral y cirugía bucal, 2014-08, Vol.19 (6), p.e562-e568</ispartof><rights>Copyright: © 2014 Medicina Oral S.L. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259371/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259371/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Saberi, Eshaghali</creatorcontrib><creatorcontrib>Kordi-Tamandani, Dor M.</creatorcontrib><creatorcontrib>Jamali, Sara</creatorcontrib><creatorcontrib>Rigi-Ladez, Mohammad A.</creatorcontrib><creatorcontrib>Augend, Arsalan</creatorcontrib><title>Analysis of methylation and mRNA expression status ofFADD andFAS genes in patients with oral squamous cell carcinoma</title><title>Medicina oral, patología oral y cirugía bucal</title><description>Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of
FADD
and
FAS
genes in risk of OSCC.
Material and Methods: we analyze the promoter methylation status of
FADD
and
FAS
genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also,
FADD
and
FAS
genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR.
Results: Aberrant promoter methylation of
FADD
and
FAS
genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both
FADD
and
FAS
genes (
P
<0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both
FADD
(
p
<0.02) and
FAS
(
p
<0.007) genes.
Conclusions: To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the
FADD
and
FAS
genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations.
Key words:
OSCC, FADD, FAS, DNA methylation, gene expression.</description><issn>1698-4447</issn><issn>1698-6946</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqljztPw0AQhE8IRMKjpN8_kOCzz3bcIFkEKxUF0J8W5xIfuofxngH_e85SGmqqXe3ON5ph7I4na5Hx8t6qvR_QrHm1SfIztuRFtVkVlSjOT7sQolywK6KPJMlKXhaXbJHmPK1SkS9ZqB2aiTSBP4BVoZsMBu0doNuDfXmuQf30gyKabxQwjLOyqbfbWdHUr3BUThFoB30ElQsE3zp0MIcC-hzR-oi0yhhocWi18xZv2MUBDanb07xmD83T2-Nu1Y_vsU8bXSIt-0FbHCbpUcu_H6c7efRfUqR5FTtl_zb4BU5GbAo</recordid><startdate>20140817</startdate><enddate>20140817</enddate><creator>Saberi, Eshaghali</creator><creator>Kordi-Tamandani, Dor M.</creator><creator>Jamali, Sara</creator><creator>Rigi-Ladez, Mohammad A.</creator><creator>Augend, Arsalan</creator><general>Medicina Oral S.L</general><scope>5PM</scope></search><sort><creationdate>20140817</creationdate><title>Analysis of methylation and mRNA expression status ofFADD andFAS genes in patients with oral squamous cell carcinoma</title><author>Saberi, Eshaghali ; Kordi-Tamandani, Dor M. ; Jamali, Sara ; Rigi-Ladez, Mohammad A. ; Augend, Arsalan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_42593713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saberi, Eshaghali</creatorcontrib><creatorcontrib>Kordi-Tamandani, Dor M.</creatorcontrib><creatorcontrib>Jamali, Sara</creatorcontrib><creatorcontrib>Rigi-Ladez, Mohammad A.</creatorcontrib><creatorcontrib>Augend, Arsalan</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Medicina oral, patología oral y cirugía bucal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saberi, Eshaghali</au><au>Kordi-Tamandani, Dor M.</au><au>Jamali, Sara</au><au>Rigi-Ladez, Mohammad A.</au><au>Augend, Arsalan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of methylation and mRNA expression status ofFADD andFAS genes in patients with oral squamous cell carcinoma</atitle><jtitle>Medicina oral, patología oral y cirugía bucal</jtitle><date>2014-08-17</date><risdate>2014</risdate><volume>19</volume><issue>6</issue><spage>e562</spage><epage>e568</epage><pages>e562-e568</pages><issn>1698-4447</issn><eissn>1698-6946</eissn><abstract>Background: Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumorigenesis. Our aims were to analyze the promoter methylation and gene expression profiles of
FADD
and
FAS
genes in risk of OSCC.
Material and Methods: we analyze the promoter methylation status of
FADD
and
FAS
genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also,
FADD
and
FAS
genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR.
Results: Aberrant promoter methylation of
FADD
and
FAS
genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both
FADD
and
FAS
genes (
P
<0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both
FADD
(
p
<0.02) and
FAS
(
p
<0.007) genes.
Conclusions: To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the
FADD
and
FAS
genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations.
Key words:
OSCC, FADD, FAS, DNA methylation, gene expression.</abstract><pub>Medicina Oral S.L</pub><pmid>25129245</pmid><doi>10.4317/medoral.19805</doi><oa>free_for_read</oa></addata></record> |
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| issn | 1698-4447 1698-6946 |
| language | eng |
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| source | PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| title | Analysis of methylation and mRNA expression status ofFADD andFAS genes in patients with oral squamous cell carcinoma |
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