Characterization of kanamycin-resistant cell lines of Nicotiana tabacum [Tobacco]
Two kanamycin-resistant variants of Nicotiana tabacum were derived by culturing seedling leaf sections on a shoot-inducing medium containing kanamycin. The variants displayed a higher resistance to the structurally related antibiotic streptomycin than to kanamycin. The resistance phenotype was expre...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1981-06, Vol.67 (6), p.1166-1168 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Owens, Lowell D. |
| description | Two kanamycin-resistant variants of Nicotiana tabacum were derived by culturing seedling leaf sections on a shoot-inducing medium containing kanamycin. The variants displayed a higher resistance to the structurally related antibiotic streptomycin than to kanamycin. The resistance phenotype was expressed when the tissue was cultured either as callus or as a differentiating tissue and was stably maintained in the absence of kanamycin. Plants regenerated from the variant lines had morphologically abnormal flowers and produced few viable seeds. These resistant lines are potentially useful for protoplast fusion or genetic modification experiments requiring selectable phenotypic markers. |
| doi_str_mv | 10.1104/pp.67.6.1166 |
| format | Article |
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The variants displayed a higher resistance to the structurally related antibiotic streptomycin than to kanamycin. The resistance phenotype was expressed when the tissue was cultured either as callus or as a differentiating tissue and was stably maintained in the absence of kanamycin. Plants regenerated from the variant lines had morphologically abnormal flowers and produced few viable seeds. 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The variants displayed a higher resistance to the structurally related antibiotic streptomycin than to kanamycin. The resistance phenotype was expressed when the tissue was cultured either as callus or as a differentiating tissue and was stably maintained in the absence of kanamycin. Plants regenerated from the variant lines had morphologically abnormal flowers and produced few viable seeds. These resistant lines are potentially useful for protoplast fusion or genetic modification experiments requiring selectable phenotypic markers.</description><subject>Agriculture</subject><subject>Antibiotics</subject><subject>Callus</subject><subject>Cell lines</subject><subject>Morphogenesis</subject><subject>Nicotiana tabacum</subject><subject>Plant growth</subject><subject>Plants</subject><subject>Seedlings</subject><subject>Sulfates</subject><subject>Sustainable agriculture</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><recordid>eNp90Utv3CAQAGBUpWo22956jCKfmku9BYN5HHKIVn1JUaKqm1NVoTHGCYltHGArbX992e4qTS85AZpvYIZB6C3BC0Iw-zBNCy4WPB84f4FmpKZVWdVMHqAZxnmPpVSH6CjGO4wxoYS9QoeZciIrNUPflrcQwCQb3G9Izo-F74p7GGHYGDeWwUYXE4ypMLbvi96NNm7FpTM-ucyKBA2Y9VD8WPm8Mf7na_Sygz7aN_t1jq4_fVwtv5QXV5-_Ls8vSsMqmsoOOqlUw4ggraxxx43AHROCK8Ya0_C2VaoWrM3VV1kxxbmSwGtpZK2oAjpHZ7t7p3Uz2NbYMQXo9RTcAGGjPTj9f2R0t_rG_9KsqmXNcv7pPj_4h7WNSQ8ubruE0fp11IJSJhUTJMt3z8r85UJSgjN8v4Mm-BiD7R6rIVhvh6WnSXOhud4OK_OTpx38w_vpZHC8A3cx-fAYZxXn8u9z-_wOvIab4KK-_k6UrLBg2VD6B2fmot0</recordid><startdate>19810601</startdate><enddate>19810601</enddate><creator>Owens, Lowell D.</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19810601</creationdate><title>Characterization of kanamycin-resistant cell lines of Nicotiana tabacum [Tobacco]</title><author>Owens, Lowell D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-faf899b4171d850f6c70f4776944bcb6dd99574d8892b41496698a658c85939a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Agriculture</topic><topic>Antibiotics</topic><topic>Callus</topic><topic>Cell lines</topic><topic>Morphogenesis</topic><topic>Nicotiana tabacum</topic><topic>Plant growth</topic><topic>Plants</topic><topic>Seedlings</topic><topic>Sulfates</topic><topic>Sustainable agriculture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Owens, Lowell D.</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Owens, Lowell D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of kanamycin-resistant cell lines of Nicotiana tabacum [Tobacco]</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1981-06-01</date><risdate>1981</risdate><volume>67</volume><issue>6</issue><spage>1166</spage><epage>1168</epage><pages>1166-1168</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Two kanamycin-resistant variants of Nicotiana tabacum were derived by culturing seedling leaf sections on a shoot-inducing medium containing kanamycin. The variants displayed a higher resistance to the structurally related antibiotic streptomycin than to kanamycin. The resistance phenotype was expressed when the tissue was cultured either as callus or as a differentiating tissue and was stably maintained in the absence of kanamycin. Plants regenerated from the variant lines had morphologically abnormal flowers and produced few viable seeds. These resistant lines are potentially useful for protoplast fusion or genetic modification experiments requiring selectable phenotypic markers.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>16661829</pmid><doi>10.1104/pp.67.6.1166</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1981-06, Vol.67 (6), p.1166-1168 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_425854 |
| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agriculture Antibiotics Callus Cell lines Morphogenesis Nicotiana tabacum Plant growth Plants Seedlings Sulfates Sustainable agriculture |
| title | Characterization of kanamycin-resistant cell lines of Nicotiana tabacum [Tobacco] |
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