Discovery and characterization of potential prognostic biomarkers for dengue hemorrhagic fever
Half a million patients are hospitalized with severe dengue every year, many of whom would die without timely, appropriate clinical intervention. The majority of dengue cases are uncomplicated; however, 2-5% progress to severe dengue. Severe dengue cases have been reported with increasing frequency...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2014-12, Vol.91 (6), p.1218-1226 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Poole-Smith, B Katherine Gilbert, Alexa Gonzalez, Andrea L Beltran, Manuela Tomashek, Kay M Ward, Brian J Hunsperger, Elizabeth A Ndao, Momar |
| description | Half a million patients are hospitalized with severe dengue every year, many of whom would die without timely, appropriate clinical intervention. The majority of dengue cases are uncomplicated; however, 2-5% progress to severe dengue. Severe dengue cases have been reported with increasing frequency over the last 30 years. To discover biomarkers for severe dengue, we used surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to analyze dengue virus positive serum samples from the acute phase of infection. Using this method, 16 proteins were identified as candidate biomarkers for severe dengue. From these 16 biomarkers, three candidates were selected for confirmation by enzyme-linked immunosorbent assay and Western blot: vitronectin (Vtn, 55.1 kDa), hemopexin (Hx, 52.4 kDa), and serotransferrin (Tf, 79.2 kDa). Vitronectin, Hx, and Tf best differentiated between dengue and severe dengue. |
| doi_str_mv | 10.4269/ajtmh.14-0193 |
| format | Article |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Biomarkers - blood Dengue virus Enzyme-Linked Immunosorbent Assay Humans Prognosis Severe Dengue - diagnosis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Discovery and characterization of potential prognostic biomarkers for dengue hemorrhagic fever |
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