Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line
Hek-293 cell line presents good production platform for recombinant therapeutic proteins, however little is known about the components that contribute to the cellular control of recombinant protein production. In this study, we generated a Hek-293 producing recombinant factor VIII (FVIII) and we eva...
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| creator | Rodrigues, Evandra Strazza Picanço-Castro, Virgínia Espanhol, Marta Regina de Andrade, Luiz Alberto Martins Palma, Patricia Vianna Bonini Kashima, Simone Fontes, Aparecida Maria Covas, Dimas Tadeu |
| description | Hek-293 cell line presents good production platform for recombinant therapeutic proteins, however little is known about the components that contribute to the cellular control of recombinant protein production. In this study, we generated a Hek-293 producing recombinant factor VIII (FVIII) and we evaluated the immunoglobulin-binding protein (BiP) and phytanoil-CoA α-hydroxylase (PAHX) expression levels which are known for diminishing FVIII production. Our analyses showed that the recombinant cell population expresses 3.1 ± 1.4 fold of BIP mRNA (P = 0.0054) and 97.8 ± 0.5 fold of PAHX mRNA (P = 0.0016) compared to nontransduced cells. The amount of these proteins was inversely correlated to the secreted FVIII. In conclusion, BIP and PAHX expression are augmented in human cells producing FVIII and they antagonize the amount of therapeutic factor VIII in the cell culture. |
| doi_str_mv | 10.1186/2193-1801-2-328 |
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In this study, we generated a Hek-293 producing recombinant factor VIII (FVIII) and we evaluated the immunoglobulin-binding protein (BiP) and phytanoil-CoA α-hydroxylase (PAHX) expression levels which are known for diminishing FVIII production. Our analyses showed that the recombinant cell population expresses 3.1 ± 1.4 fold of BIP mRNA (P = 0.0054) and 97.8 ± 0.5 fold of PAHX mRNA (P = 0.0016) compared to nontransduced cells. The amount of these proteins was inversely correlated to the secreted FVIII. In conclusion, BIP and PAHX expression are augmented in human cells producing FVIII and they antagonize the amount of therapeutic factor VIII in the cell culture.</description><identifier>ISSN: 2193-1801</identifier><identifier>EISSN: 2193-1801</identifier><identifier>DOI: 10.1186/2193-1801-2-328</identifier><identifier>PMID: 25530931</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Humanities and Social Sciences ; Microbiology and Biotechnology ; multidisciplinary ; Science ; Science (multidisciplinary)</subject><ispartof>SpringerPlus, 2013-07, Vol.2 (1), p.328-328, Article 328</ispartof><rights>Rodrigues et al.; licensee Springer. 2013. This article is published under license to BioMed Central Ltd. 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Picanço-Castro, Virgínia ; Espanhol, Marta Regina ; de Andrade, Luiz Alberto Martins ; Palma, Patricia Vianna Bonini ; Kashima, Simone ; Fontes, Aparecida Maria ; Covas, Dimas Tadeu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b521t-6e6164e92f58124ff6abb880e9706988fac90a9f457b2632abd040789e1f43513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Humanities and Social Sciences</topic><topic>Microbiology and Biotechnology</topic><topic>multidisciplinary</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, Evandra Strazza</creatorcontrib><creatorcontrib>Picanço-Castro, Virgínia</creatorcontrib><creatorcontrib>Espanhol, Marta Regina</creatorcontrib><creatorcontrib>de Andrade, Luiz Alberto Martins</creatorcontrib><creatorcontrib>Palma, Patricia Vianna Bonini</creatorcontrib><creatorcontrib>Kashima, Simone</creatorcontrib><creatorcontrib>Fontes, Aparecida Maria</creatorcontrib><creatorcontrib>Covas, Dimas Tadeu</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Computer Science Collection</collection><collection>Computer Science Database</collection><collection>Materials Science Database</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>SpringerPlus</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodrigues, Evandra Strazza</au><au>Picanço-Castro, Virgínia</au><au>Espanhol, Marta Regina</au><au>de Andrade, Luiz Alberto Martins</au><au>Palma, Patricia Vianna Bonini</au><au>Kashima, Simone</au><au>Fontes, Aparecida Maria</au><au>Covas, Dimas Tadeu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line</atitle><jtitle>SpringerPlus</jtitle><stitle>SpringerPlus</stitle><addtitle>Springerplus</addtitle><date>2013-07-18</date><risdate>2013</risdate><volume>2</volume><issue>1</issue><spage>328</spage><epage>328</epage><pages>328-328</pages><artnum>328</artnum><issn>2193-1801</issn><eissn>2193-1801</eissn><abstract>Hek-293 cell line presents good production platform for recombinant therapeutic proteins, however little is known about the components that contribute to the cellular control of recombinant protein production. In this study, we generated a Hek-293 producing recombinant factor VIII (FVIII) and we evaluated the immunoglobulin-binding protein (BiP) and phytanoil-CoA α-hydroxylase (PAHX) expression levels which are known for diminishing FVIII production. Our analyses showed that the recombinant cell population expresses 3.1 ± 1.4 fold of BIP mRNA (P = 0.0054) and 97.8 ± 0.5 fold of PAHX mRNA (P = 0.0016) compared to nontransduced cells. The amount of these proteins was inversely correlated to the secreted FVIII. In conclusion, BIP and PAHX expression are augmented in human cells producing FVIII and they antagonize the amount of therapeutic factor VIII in the cell culture.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>25530931</pmid><doi>10.1186/2193-1801-2-328</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Humanities and Social Sciences Microbiology and Biotechnology multidisciplinary Science Science (multidisciplinary) |
| title | Quantitative correlation between transcriptional levels of ER chaperone, peroximal protein and FVIII productivity in human Hek-293 cell line |
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