Spatially Defined EGF Receptor Activation Reveals an F-Actin-Dependent Phospho-Erk Signaling Complex

We investigated the association of signaling proteins with epidermal growth factor (EGF) receptors (EGFR) using biotinylated EGF bound to streptavidin that is covalently coupled in an ordered array of micron-sized features on silicon surfaces. Using NIH-3T3 cells stably expressing EGFR, we observe c...

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Veröffentlicht in:	Biophysical journal 2014-12, Vol.107 (11), p.2639-2651
Hauptverfasser:	Singhai, Amit, Wakefield, Devin L., Bryant, Kirsten L., Hammes, Stephen R., Holowka, David, Baird, Barbara
Format:	Artikel
Sprache:	eng
Schlagworte:	Actin Cytoskeleton - drug effects Actin Cytoskeleton - metabolism Actins - metabolism Animals Biophysics Cell Biophysics Cell Membrane - drug effects Cell Membrane - metabolism Correlation analysis Dynamin II - metabolism Epidermal growth factor Epidermal Growth Factor - pharmacology Integrin beta1 - metabolism Ligands MAP Kinase Signaling System - drug effects Membranes Mice NIH 3T3 Cells Paxillin - metabolism Phosphatidylinositol 4,5-Diphosphate - metabolism Phosphorylation Phosphorylation - drug effects Phosphotyrosine - metabolism Polymerization - drug effects Protein expression Receptor, Epidermal Growth Factor - metabolism Signal transduction Sirolimus - pharmacology
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