NrsZ: a novel, processed, nitrogen‐dependent, small non‐coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence
The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non‐coding RNA: NrsZ (nitrogen‐regulated sRNA). We show that under nitrogen lim...
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| Veröffentlicht in: | Environmental microbiology 2014-04, Vol.16 (4), p.1053-1068 |
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| creator | Wenner, Nicolas Maes, Alexandre Cotado‐Sampayo, Marta Lapouge, Karine |
| description | The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non‐coding RNA: NrsZ (nitrogen‐regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two‐component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post‐transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions. |
| doi_str_mv | 10.1111/1462-2920.12272 |
| format | Article |
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Here, we report the discovery and characterization of a novel small non‐coding RNA: NrsZ (nitrogen‐regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two‐component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post‐transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions.</description><identifier>ISSN: 1462-2912</identifier><identifier>EISSN: 1462-2920</identifier><identifier>DOI: 10.1111/1462-2920.12272</identifier><identifier>PMID: 24308329</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Gene Expression Regulation, Bacterial ; genes ; Glycolipids - metabolism ; nitrogen ; Nitrogen - metabolism ; nucleotides ; nutrients ; pathogens ; Pseudomonas ; Pseudomonas aeruginosa - pathogenicity ; Pseudomonas aeruginosa - physiology ; RNA ; RNA, Small Untranslated - chemistry ; RNA, Small Untranslated - genetics ; surfactants ; virulence ; Virulence - genetics ; Virulence Factors - genetics</subject><ispartof>Environmental microbiology, 2014-04, Vol.16 (4), p.1053-1068</ispartof><rights>2013 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.</rights><rights>Copyright © 2014 John Wiley & Sons Ltd 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1462-2920.12272$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1462-2920.12272$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24308329$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wenner, Nicolas</creatorcontrib><creatorcontrib>Maes, Alexandre</creatorcontrib><creatorcontrib>Cotado‐Sampayo, Marta</creatorcontrib><creatorcontrib>Lapouge, Karine</creatorcontrib><title>NrsZ: a novel, processed, nitrogen‐dependent, small non‐coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence</title><title>Environmental microbiology</title><addtitle>Environ Microbiol</addtitle><description>The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non‐coding RNA: NrsZ (nitrogen‐regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two‐component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post‐transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions.</description><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>beta-Galactosidase - genetics</subject><subject>beta-Galactosidase - metabolism</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>genes</subject><subject>Glycolipids - metabolism</subject><subject>nitrogen</subject><subject>Nitrogen - metabolism</subject><subject>nucleotides</subject><subject>nutrients</subject><subject>pathogens</subject><subject>Pseudomonas</subject><subject>Pseudomonas aeruginosa - pathogenicity</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>RNA</subject><subject>RNA, Small Untranslated - chemistry</subject><subject>RNA, Small Untranslated - genetics</subject><subject>surfactants</subject><subject>virulence</subject><subject>Virulence - genetics</subject><subject>Virulence Factors - genetics</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>WIN</sourceid><sourceid>EIF</sourceid><recordid>eNpVkU1uFDEQhVsIREJgzQ58gGnwX_-xQBpFIRMUJhEEgdhYNe1yx9Bjj-zugYgNR-CMnARPBlrgjctV33uS62XZY0afsXSeM1nynDc8PTmv-J3scOrcnWrGD7IHMX6mlFWiovezAy4FrQVvDrPvyxA_vSBAnN9iPyOb4FuMEfWMODsE36H79eOnxg06jW6YkbiGvk_0rt16bV1H3i7nZLiGgQTsxh4GjOQy4qj92juIBDCMnXU-ArmcXzCytWHs0bX4MLtnoI_46M99lF29Ork6XuTnF6dnx_Pz3PCK8VyuUNQryWiJQjZFpcHQEmpspZRGC6mFFlWrDTRN3QAio5pXRpRGo1lxKo6yl3vbzbhao27TNwL0ahPsGsKN8mDV_xNnr1Xnt0ryQqS1JoMn_xpMyr9bTECxB77aHm-mOaNqF5LaxaB2kajbkNTJm7PbIunyvc7GAb9NOghfVJmSKtSH5amSy491U79eqEXin-55A15BF2xU799xyiSltCgLwcVv-hCgtA</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Wenner, Nicolas</creator><creator>Maes, Alexandre</creator><creator>Cotado‐Sampayo, Marta</creator><creator>Lapouge, Karine</creator><general>Blackwell Publishing Ltd</general><general>BlackWell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>201404</creationdate><title>NrsZ: a novel, processed, nitrogen‐dependent, small non‐coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence</title><author>Wenner, Nicolas ; Maes, Alexandre ; Cotado‐Sampayo, Marta ; Lapouge, Karine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f2712-4be38b4106e34957daf06a8ec444fd34d3d37cdfa9989aee10d27f36fdefb203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>beta-Galactosidase - genetics</topic><topic>beta-Galactosidase - metabolism</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>genes</topic><topic>Glycolipids - metabolism</topic><topic>nitrogen</topic><topic>Nitrogen - metabolism</topic><topic>nucleotides</topic><topic>nutrients</topic><topic>pathogens</topic><topic>Pseudomonas</topic><topic>Pseudomonas aeruginosa - pathogenicity</topic><topic>Pseudomonas aeruginosa - physiology</topic><topic>RNA</topic><topic>RNA, Small Untranslated - chemistry</topic><topic>RNA, Small Untranslated - genetics</topic><topic>surfactants</topic><topic>virulence</topic><topic>Virulence - genetics</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wenner, Nicolas</creatorcontrib><creatorcontrib>Maes, Alexandre</creatorcontrib><creatorcontrib>Cotado‐Sampayo, Marta</creatorcontrib><creatorcontrib>Lapouge, Karine</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library (Open Access Collection)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wenner, Nicolas</au><au>Maes, Alexandre</au><au>Cotado‐Sampayo, Marta</au><au>Lapouge, Karine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NrsZ: a novel, processed, nitrogen‐dependent, small non‐coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2014-04</date><risdate>2014</risdate><volume>16</volume><issue>4</issue><spage>1053</spage><epage>1068</epage><pages>1053-1068</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>The opportunistic pathogen Pseudomonas aeruginosa PAO1 has a remarkable capacity to adapt to various environments and to survive with limited nutrients. Here, we report the discovery and characterization of a novel small non‐coding RNA: NrsZ (nitrogen‐regulated sRNA). We show that under nitrogen limitation, NrsZ is induced by the NtrB/C two‐component system, an important regulator of nitrogen assimilation and P. aeruginosa's swarming motility, in concert with the alternative sigma factor RpoN. Furthermore, we demonstrate that NrsZ modulates P. aeruginosa motility by controlling the production of rhamnolipid surfactants, virulence factors notably needed for swarming motility. This regulation takes place through the post‐transcriptional control of rhlA, a gene essential for rhamnolipids synthesis. Interestingly, we also observed that NrsZ is processed in three similar short modules, and that the first short module encompassing the first 60 nucleotides is sufficient for NrsZ regulatory functions.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>24308329</pmid><doi>10.1111/1462-2920.12272</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Proteins - genetics Bacterial Proteins - metabolism beta-Galactosidase - genetics beta-Galactosidase - metabolism Gene Expression Regulation, Bacterial genes Glycolipids - metabolism nitrogen Nitrogen - metabolism nucleotides nutrients pathogens Pseudomonas Pseudomonas aeruginosa - pathogenicity Pseudomonas aeruginosa - physiology RNA RNA, Small Untranslated - chemistry RNA, Small Untranslated - genetics surfactants virulence Virulence - genetics Virulence Factors - genetics |
| title | NrsZ: a novel, processed, nitrogen‐dependent, small non‐coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence |
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