Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice
To investigate the change in expression of p53, Bcl-2, and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy and thermochemoradiotherapy. Human colon cancer cell line (HT29) was transplanted into t...
Gespeichert in:
| Veröffentlicht in: | World journal of gastroenterology : WJG 2007-08, Vol.13 (32), p.4365-4371 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4371 |
|---|---|
| container_issue | 32 |
| container_start_page | 4365 |
| container_title | World journal of gastroenterology : WJG |
| container_volume | 13 |
| creator | Liang, Han Zhan, Hong-Jie Wang, Bao-Gui Pan, Yuan Hao, Xi-Shan |
| description | To investigate the change in expression of p53, Bcl-2, and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy and thermochemoradiotherapy.
Human colon cancer cell line (HT29) was transplanted into the hind limbs of nude mice. Under laboratory simulated conditions of hyperthermia (43 centigrade, 60 min), the actual radiation doses and doses of mitomycin C (MMC) were calculated in reference to the clinical radiotherapy for human rectal cancer and chemotherapy prescription for colon cancer. The mice were divided into 6 groups according to the treatment approaches: hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy, and thermochemoradiotherapy. The mice were sacrificed at different time points and the tumor tissue was taken for further procedures. The morphologic changes in membrane, cytoplasm and nuclei of tumor cells of p53, Bcl-2, and Bax after treatment, were observed by immunohistochemistry staining.
All of the six treatment modalities down-regulated the expression of p53, Bcl-2 and up-regulated the expression of Bax at different levels. The combined therapy of hyperthermia, with chemotherapy, and/or irradiation showed a greater effect on down-regulating the expression of p53 (0.208 +/- 0.009 vs 0.155 +/- 0.0115, P < 0.01) and Bcl-2 (0.086 +/- 0.010 vs 0.026 +/- 0.0170, P < 0.01) and up-regulating Bax expression (0.091 +/- 0.0013 vs 0.207 +/- 0.027, P < 0.01) compared with any single therapy.
Hyperthermia enhances the effect of radio- and chemotherapy on tumors by changing the expression of apoptosis genes, such as p53, Bcl-2 and Bax. |
| doi_str_mv | 10.3748/wjg.v13.i32.4365 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4250866</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>wjg200732015</wanfj_id><sourcerecordid>wjg200732015</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-e5e0f8f6f94ddbc6a07005fa2967b439419881500039c9f8e685c9c5fd0924453</originalsourceid><addsrcrecordid>eNpVkU-P0zAQxS0EYsvCnRPyAXHaFP9LYl-QUMUC0kpc4Gy5zrhxldjBTnbpZ-BL46jVAifL9sxv3ryH0GtKtrwV8v3D8bC9p3zrOdsK3tRP0IYxqiomBXmKNpSQtlKctVfoRc5HQhjnNXuOrmjbEtlQvkG_d70JB8A-YPg1JcjZx4Cjw2aK0xyzz_gAATI2boaE-9MEae4hjd7cYNvDGNebmU7YhA4n0_nHh4Lsl9EEbONQmNYEWwhzMiFPgwkzdKVkjjgsHeDRW3iJnjkzZHh1Oa_Rj9tP33dfqrtvn7_uPt5VVjA-V1ADcdI1Tomu29vGkJaQ2hmmmnYvuBJUSUlrQghXVjkJjaytsrXriGJC1PwafThzp2U_QmchFFGDnpIfTTrpaLz-_yf4Xh_ivRasLrY1BfD2DHgwwRX79DEuKRTJugTCiumcEbrOeXeZk-LPBfKsR58tDGV5iEvWjaSSSyFKITkX2hRzTuAetVCi16BXri5B6xK0XoMuLW_-3eFvwyVZ_geIPKjh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68183844</pqid></control><display><type>article</type><title>Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Liang, Han ; Zhan, Hong-Jie ; Wang, Bao-Gui ; Pan, Yuan ; Hao, Xi-Shan</creator><creatorcontrib>Liang, Han ; Zhan, Hong-Jie ; Wang, Bao-Gui ; Pan, Yuan ; Hao, Xi-Shan</creatorcontrib><description>To investigate the change in expression of p53, Bcl-2, and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy and thermochemoradiotherapy.
Human colon cancer cell line (HT29) was transplanted into the hind limbs of nude mice. Under laboratory simulated conditions of hyperthermia (43 centigrade, 60 min), the actual radiation doses and doses of mitomycin C (MMC) were calculated in reference to the clinical radiotherapy for human rectal cancer and chemotherapy prescription for colon cancer. The mice were divided into 6 groups according to the treatment approaches: hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy, and thermochemoradiotherapy. The mice were sacrificed at different time points and the tumor tissue was taken for further procedures. The morphologic changes in membrane, cytoplasm and nuclei of tumor cells of p53, Bcl-2, and Bax after treatment, were observed by immunohistochemistry staining.
All of the six treatment modalities down-regulated the expression of p53, Bcl-2 and up-regulated the expression of Bax at different levels. The combined therapy of hyperthermia, with chemotherapy, and/or irradiation showed a greater effect on down-regulating the expression of p53 (0.208 +/- 0.009 vs 0.155 +/- 0.0115, P < 0.01) and Bcl-2 (0.086 +/- 0.010 vs 0.026 +/- 0.0170, P < 0.01) and up-regulating Bax expression (0.091 +/- 0.0013 vs 0.207 +/- 0.027, P < 0.01) compared with any single therapy.
Hyperthermia enhances the effect of radio- and chemotherapy on tumors by changing the expression of apoptosis genes, such as p53, Bcl-2 and Bax.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v13.i32.4365</identifier><identifier>PMID: 17708613</identifier><language>eng</language><publisher>United States: Department of Gastrointestinal Oncological Surgery, Tianjin Cancer Hospital, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China</publisher><subject>Animals ; Apoptosis - genetics ; bcl-2-Associated X Protein - genetics ; bcl-2-Associated X Protein - metabolism ; Cell Line, Tumor ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Colonic Neoplasms - therapy ; Combined Modality Therapy ; Drug Therapy - methods ; Female ; Humans ; Hyperthermia, Induced - methods ; Male ; Mice ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Radiotherapy - methods ; Rapid Communication ; Tumor Suppressor Protein p53 - genetics ; Tumor Suppressor Protein p53 - metabolism ; Xenograft Model Antitumor Assays</subject><ispartof>World journal of gastroenterology : WJG, 2007-08, Vol.13 (32), p.4365-4371</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2007 Baishideng Publishing Group Co., Limited. All rights reserved. 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-e5e0f8f6f94ddbc6a07005fa2967b439419881500039c9f8e685c9c5fd0924453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250866/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250866/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17708613$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Han</creatorcontrib><creatorcontrib>Zhan, Hong-Jie</creatorcontrib><creatorcontrib>Wang, Bao-Gui</creatorcontrib><creatorcontrib>Pan, Yuan</creatorcontrib><creatorcontrib>Hao, Xi-Shan</creatorcontrib><title>Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To investigate the change in expression of p53, Bcl-2, and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy and thermochemoradiotherapy.
Human colon cancer cell line (HT29) was transplanted into the hind limbs of nude mice. Under laboratory simulated conditions of hyperthermia (43 centigrade, 60 min), the actual radiation doses and doses of mitomycin C (MMC) were calculated in reference to the clinical radiotherapy for human rectal cancer and chemotherapy prescription for colon cancer. The mice were divided into 6 groups according to the treatment approaches: hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy, and thermochemoradiotherapy. The mice were sacrificed at different time points and the tumor tissue was taken for further procedures. The morphologic changes in membrane, cytoplasm and nuclei of tumor cells of p53, Bcl-2, and Bax after treatment, were observed by immunohistochemistry staining.
All of the six treatment modalities down-regulated the expression of p53, Bcl-2 and up-regulated the expression of Bax at different levels. The combined therapy of hyperthermia, with chemotherapy, and/or irradiation showed a greater effect on down-regulating the expression of p53 (0.208 +/- 0.009 vs 0.155 +/- 0.0115, P < 0.01) and Bcl-2 (0.086 +/- 0.010 vs 0.026 +/- 0.0170, P < 0.01) and up-regulating Bax expression (0.091 +/- 0.0013 vs 0.207 +/- 0.027, P < 0.01) compared with any single therapy.
Hyperthermia enhances the effect of radio- and chemotherapy on tumors by changing the expression of apoptosis genes, such as p53, Bcl-2 and Bax.</description><subject>Animals</subject><subject>Apoptosis - genetics</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colonic Neoplasms - therapy</subject><subject>Combined Modality Therapy</subject><subject>Drug Therapy - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Hyperthermia, Induced - methods</subject><subject>Male</subject><subject>Mice</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Radiotherapy - methods</subject><subject>Rapid Communication</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU-P0zAQxS0EYsvCnRPyAXHaFP9LYl-QUMUC0kpc4Gy5zrhxldjBTnbpZ-BL46jVAifL9sxv3ryH0GtKtrwV8v3D8bC9p3zrOdsK3tRP0IYxqiomBXmKNpSQtlKctVfoRc5HQhjnNXuOrmjbEtlQvkG_d70JB8A-YPg1JcjZx4Cjw2aK0xyzz_gAATI2boaE-9MEae4hjd7cYNvDGNebmU7YhA4n0_nHh4Lsl9EEbONQmNYEWwhzMiFPgwkzdKVkjjgsHeDRW3iJnjkzZHh1Oa_Rj9tP33dfqrtvn7_uPt5VVjA-V1ADcdI1Tomu29vGkJaQ2hmmmnYvuBJUSUlrQghXVjkJjaytsrXriGJC1PwafThzp2U_QmchFFGDnpIfTTrpaLz-_yf4Xh_ivRasLrY1BfD2DHgwwRX79DEuKRTJugTCiumcEbrOeXeZk-LPBfKsR58tDGV5iEvWjaSSSyFKITkX2hRzTuAetVCi16BXri5B6xK0XoMuLW_-3eFvwyVZ_geIPKjh</recordid><startdate>20070828</startdate><enddate>20070828</enddate><creator>Liang, Han</creator><creator>Zhan, Hong-Jie</creator><creator>Wang, Bao-Gui</creator><creator>Pan, Yuan</creator><creator>Hao, Xi-Shan</creator><general>Department of Gastrointestinal Oncological Surgery, Tianjin Cancer Hospital, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China</general><general>Baishideng Publishing Group Co., Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20070828</creationdate><title>Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice</title><author>Liang, Han ; Zhan, Hong-Jie ; Wang, Bao-Gui ; Pan, Yuan ; Hao, Xi-Shan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-e5e0f8f6f94ddbc6a07005fa2967b439419881500039c9f8e685c9c5fd0924453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Apoptosis - genetics</topic><topic>bcl-2-Associated X Protein - genetics</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colonic Neoplasms - therapy</topic><topic>Combined Modality Therapy</topic><topic>Drug Therapy - methods</topic><topic>Female</topic><topic>Humans</topic><topic>Hyperthermia, Induced - methods</topic><topic>Male</topic><topic>Mice</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Radiotherapy - methods</topic><topic>Rapid Communication</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>online_resources</toplevel><creatorcontrib>Liang, Han</creatorcontrib><creatorcontrib>Zhan, Hong-Jie</creatorcontrib><creatorcontrib>Wang, Bao-Gui</creatorcontrib><creatorcontrib>Pan, Yuan</creatorcontrib><creatorcontrib>Hao, Xi-Shan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Han</au><au>Zhan, Hong-Jie</au><au>Wang, Bao-Gui</au><au>Pan, Yuan</au><au>Hao, Xi-Shan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2007-08-28</date><risdate>2007</risdate><volume>13</volume><issue>32</issue><spage>4365</spage><epage>4371</epage><pages>4365-4371</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To investigate the change in expression of p53, Bcl-2, and Bax genes in human colon cancer cells transplanted into nude mice after hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy and thermochemoradiotherapy.
Human colon cancer cell line (HT29) was transplanted into the hind limbs of nude mice. Under laboratory simulated conditions of hyperthermia (43 centigrade, 60 min), the actual radiation doses and doses of mitomycin C (MMC) were calculated in reference to the clinical radiotherapy for human rectal cancer and chemotherapy prescription for colon cancer. The mice were divided into 6 groups according to the treatment approaches: hyperthermia, chemotherapy, radiotherapy, thermochemotherapy, thermoradiotherapy, and thermochemoradiotherapy. The mice were sacrificed at different time points and the tumor tissue was taken for further procedures. The morphologic changes in membrane, cytoplasm and nuclei of tumor cells of p53, Bcl-2, and Bax after treatment, were observed by immunohistochemistry staining.
All of the six treatment modalities down-regulated the expression of p53, Bcl-2 and up-regulated the expression of Bax at different levels. The combined therapy of hyperthermia, with chemotherapy, and/or irradiation showed a greater effect on down-regulating the expression of p53 (0.208 +/- 0.009 vs 0.155 +/- 0.0115, P < 0.01) and Bcl-2 (0.086 +/- 0.010 vs 0.026 +/- 0.0170, P < 0.01) and up-regulating Bax expression (0.091 +/- 0.0013 vs 0.207 +/- 0.027, P < 0.01) compared with any single therapy.
Hyperthermia enhances the effect of radio- and chemotherapy on tumors by changing the expression of apoptosis genes, such as p53, Bcl-2 and Bax.</abstract><cop>United States</cop><pub>Department of Gastrointestinal Oncological Surgery, Tianjin Cancer Hospital, Tianjin Medical University, Key Laboratory of Cancer Prevention and Therapy, Tianjin 300060, China</pub><pmid>17708613</pmid><doi>10.3748/wjg.v13.i32.4365</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2007-08, Vol.13 (32), p.4365-4371 |
| issn | 1007-9327 2219-2840 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4250866 |
| source | MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Apoptosis - genetics bcl-2-Associated X Protein - genetics bcl-2-Associated X Protein - metabolism Cell Line, Tumor Colonic Neoplasms - metabolism Colonic Neoplasms - pathology Colonic Neoplasms - therapy Combined Modality Therapy Drug Therapy - methods Female Humans Hyperthermia, Induced - methods Male Mice Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Radiotherapy - methods Rapid Communication Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism Xenograft Model Antitumor Assays |
| title | Change in expression of apoptosis genes after hyperthermia, chemotherapy and radiotherapy in human colon cancer transplanted into nude mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T17%3A59%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Change%20in%20expression%20of%20apoptosis%20genes%20after%20hyperthermia,%20chemotherapy%20and%20radiotherapy%20in%20human%20colon%20cancer%20transplanted%20into%20nude%20mice&rft.jtitle=World%20journal%20of%20gastroenterology%20:%20WJG&rft.au=Liang,%20Han&rft.date=2007-08-28&rft.volume=13&rft.issue=32&rft.spage=4365&rft.epage=4371&rft.pages=4365-4371&rft.issn=1007-9327&rft.eissn=2219-2840&rft_id=info:doi/10.3748/wjg.v13.i32.4365&rft_dat=%3Cwanfang_jour_pubme%3Ewjg200732015%3C/wanfang_jour_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=68183844&rft_id=info:pmid/17708613&rft_wanfj_id=wjg200732015&rfr_iscdi=true |