Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721
AIM: To investigate the survivin gene expression in human hepatocellular carcinoma cell line SMMC-7721 and the effects of survivin gene RNA interference (RNAi) on cell apoptosis and biological behaviors of SMMC-7721 cells.METHODS: Eukaryotic expression vector of survivin gene RNAi and recombinant pl...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2005-02, Vol.11 (5), p.756-759 |
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| creator | Cheng, Sheng-Quan Wang, Wen-Liang Yan, Wei Li, Qing-Long Wang, Li Wang, Wen-Yong |
| description | AIM: To investigate the survivin gene expression in human hepatocellular carcinoma cell line SMMC-7721 and the effects of survivin gene RNA interference (RNAi) on cell apoptosis and biological behaviors of SMMC-7721 cells.METHODS: Eukaryotic expression vector of survivin gene RNAi and recombinant plasmid pSuppressorNeo-survivin (pSuNeo-SVV), were constructed by ligating into the vector, pSupperssorNeo (pSuNeo) digested with restriction enzymes Xba Ⅰ and Sal Ⅰ and the designed double-chain RNAi primers. A cell model of SMMC-7721 after treatment with RNAi was prepared by transfecting SMMC-7721 cells with the lipofectin transfection method. Strept-avidin-biotin-complex (SABC) immunohistochemical staining and RT-PCR were used to detect survivin gene expressions in SMMC-7721 cells. Flow cytometry was used for the cell cycle analysis. Transmission electron microscopy was performed to determine whether RNAi induced cell apoptosis, and the method of measuring the cell growth curve was utilized to study the growth of SMMC-7721 cells before and after treatment with RNAi.RESULTS: The eukaryotic expression vector of survivin gene RNAi and pSuNeo-SW, were constructed successfully. The expression level of survivin gene in SMMC-7721 cells was observed. After the treatment of RNAi, the expression of survivin gene in SMMC-7721 cells was almost absent, apoptosis index was increased by 15.6%, and the number of cells was decreased in G2/M phase and the cell growth was inhibited.CONCLUSION: RNAi can exert a knockdown of survivin gene expression in SMMC-7721 cells, and induce apoptosis and inhibit the growth of carcinoma cells. |
| doi_str_mv | 10.3748/wjg.v11.i5.756 |
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| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4250756</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>11888603</cqvip_id><wanfj_id>wjg200505031</wanfj_id><sourcerecordid>wjg200505031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c444t-64d2b4449f486ea30e821722ffb4c1dc55f9bc35f6b4c2f98b0f6a910b5849713</originalsourceid><addsrcrecordid>eNpVkduL1DAUxoMo7rj66qMEEd9ac22bF2EZvOGugpfnkKZJJ7Nt0k3aGfe_N2UGLwRyOfn4ne-cA8BzjEpas-bNcd-XB4xLx8uaVw_AhhAsCtIw9BBsMEJ1ISipL8CTlPYIEUo5eQwuMK84b6jYgOWzD_q2C0cPg4VpiQd3cB72xhtofk3RpOSCh-09_PblykHnu0WbBNUUpjkkl3IE7pZR5d1Mag7aDMMyqAi1itr5MCq4huDgMvD7zc22qGuCn4JHVg3JPDufl-Dn-3c_th-L668fPm2vrgvNGJuLinWkzTdhWVMZRZFpCK4JsbZlGneacytaTbmt8ptY0bTIVkpg1PKGiRrTS_D2xJ2WdjSdNn6OapBTdKOK9zIoJ___8W4n-3CQjHCU-5kBr06Ao_JW-V7uwxJ9tixz4wlCPC-65nl9zhPD3WLSLEeX1rqVN2FJsqppLQRhWViehDqGlKKxf7xgJNeBrlyZByodlycDL_6t4K_8PMEseHkm7oLv71z22Cp9a91gJMZN01SI0t8-Sqnz</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67379924</pqid></control><display><type>article</type><title>Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Cheng, Sheng-Quan ; Wang, Wen-Liang ; Yan, Wei ; Li, Qing-Long ; Wang, Li ; Wang, Wen-Yong</creator><creatorcontrib>Cheng, Sheng-Quan ; Wang, Wen-Liang ; Yan, Wei ; Li, Qing-Long ; Wang, Li ; Wang, Wen-Yong</creatorcontrib><description>AIM: To investigate the survivin gene expression in human hepatocellular carcinoma cell line SMMC-7721 and the effects of survivin gene RNA interference (RNAi) on cell apoptosis and biological behaviors of SMMC-7721 cells.METHODS: Eukaryotic expression vector of survivin gene RNAi and recombinant plasmid pSuppressorNeo-survivin (pSuNeo-SVV), were constructed by ligating into the vector, pSupperssorNeo (pSuNeo) digested with restriction enzymes Xba Ⅰ and Sal Ⅰ and the designed double-chain RNAi primers. A cell model of SMMC-7721 after treatment with RNAi was prepared by transfecting SMMC-7721 cells with the lipofectin transfection method. Strept-avidin-biotin-complex (SABC) immunohistochemical staining and RT-PCR were used to detect survivin gene expressions in SMMC-7721 cells. Flow cytometry was used for the cell cycle analysis. Transmission electron microscopy was performed to determine whether RNAi induced cell apoptosis, and the method of measuring the cell growth curve was utilized to study the growth of SMMC-7721 cells before and after treatment with RNAi.RESULTS: The eukaryotic expression vector of survivin gene RNAi and pSuNeo-SW, were constructed successfully. The expression level of survivin gene in SMMC-7721 cells was observed. After the treatment of RNAi, the expression of survivin gene in SMMC-7721 cells was almost absent, apoptosis index was increased by 15.6%, and the number of cells was decreased in G2/M phase and the cell growth was inhibited.CONCLUSION: RNAi can exert a knockdown of survivin gene expression in SMMC-7721 cells, and induce apoptosis and inhibit the growth of carcinoma cells.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v11.i5.756</identifier><identifier>PMID: 15655839</identifier><language>eng</language><publisher>United States: Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China%Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China</publisher><subject>Apoptosis ; Brief Reports ; Carcinoma, Hepatocellular - physiopathology ; Carcinoma, Hepatocellular - therapy ; Cell Division ; Cell Line, Tumor - physiology ; Cell Line, Tumor - ultrastructure ; Flow Cytometry ; Gene Expression ; Genetic Therapy - methods ; Humans ; In Vitro Techniques ; Inhibitor of Apoptosis Proteins ; Liver Neoplasms - physiopathology ; Liver Neoplasms - therapy ; Microscopy, Electron, Transmission ; Microtubule-Associated Proteins - genetics ; Neoplasm Proteins ; Restriction Mapping ; RNA, Small Interfering ; RNAi ; SMMC-7721 ; Survivin ; 基因表达 ; 抑制作用 ; 细胞凋亡 ; 肝细胞癌 ; 肿瘤细胞系统 ; 诱导作用</subject><ispartof>World journal of gastroenterology : WJG, 2005-02, Vol.11 (5), p.756-759</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2005 Baishideng Publishing Group Inc. All rights reserved. 2005</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-64d2b4449f486ea30e821722ffb4c1dc55f9bc35f6b4c2f98b0f6a910b5849713</citedby><cites>FETCH-LOGICAL-c444t-64d2b4449f486ea30e821722ffb4c1dc55f9bc35f6b4c2f98b0f6a910b5849713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250756/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250756/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15655839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheng, Sheng-Quan</creatorcontrib><creatorcontrib>Wang, Wen-Liang</creatorcontrib><creatorcontrib>Yan, Wei</creatorcontrib><creatorcontrib>Li, Qing-Long</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Wang, Wen-Yong</creatorcontrib><title>Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM: To investigate the survivin gene expression in human hepatocellular carcinoma cell line SMMC-7721 and the effects of survivin gene RNA interference (RNAi) on cell apoptosis and biological behaviors of SMMC-7721 cells.METHODS: Eukaryotic expression vector of survivin gene RNAi and recombinant plasmid pSuppressorNeo-survivin (pSuNeo-SVV), were constructed by ligating into the vector, pSupperssorNeo (pSuNeo) digested with restriction enzymes Xba Ⅰ and Sal Ⅰ and the designed double-chain RNAi primers. A cell model of SMMC-7721 after treatment with RNAi was prepared by transfecting SMMC-7721 cells with the lipofectin transfection method. Strept-avidin-biotin-complex (SABC) immunohistochemical staining and RT-PCR were used to detect survivin gene expressions in SMMC-7721 cells. Flow cytometry was used for the cell cycle analysis. Transmission electron microscopy was performed to determine whether RNAi induced cell apoptosis, and the method of measuring the cell growth curve was utilized to study the growth of SMMC-7721 cells before and after treatment with RNAi.RESULTS: The eukaryotic expression vector of survivin gene RNAi and pSuNeo-SW, were constructed successfully. The expression level of survivin gene in SMMC-7721 cells was observed. After the treatment of RNAi, the expression of survivin gene in SMMC-7721 cells was almost absent, apoptosis index was increased by 15.6%, and the number of cells was decreased in G2/M phase and the cell growth was inhibited.CONCLUSION: RNAi can exert a knockdown of survivin gene expression in SMMC-7721 cells, and induce apoptosis and inhibit the growth of carcinoma cells.</description><subject>Apoptosis</subject><subject>Brief Reports</subject><subject>Carcinoma, Hepatocellular - physiopathology</subject><subject>Carcinoma, Hepatocellular - therapy</subject><subject>Cell Division</subject><subject>Cell Line, Tumor - physiology</subject><subject>Cell Line, Tumor - ultrastructure</subject><subject>Flow Cytometry</subject><subject>Gene Expression</subject><subject>Genetic Therapy - methods</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Inhibitor of Apoptosis Proteins</subject><subject>Liver Neoplasms - physiopathology</subject><subject>Liver Neoplasms - therapy</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Neoplasm Proteins</subject><subject>Restriction Mapping</subject><subject>RNA, Small Interfering</subject><subject>RNAi</subject><subject>SMMC-7721</subject><subject>Survivin</subject><subject>基因表达</subject><subject>抑制作用</subject><subject>细胞凋亡</subject><subject>肝细胞癌</subject><subject>肿瘤细胞系统</subject><subject>诱导作用</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkduL1DAUxoMo7rj66qMEEd9ac22bF2EZvOGugpfnkKZJJ7Nt0k3aGfe_N2UGLwRyOfn4ne-cA8BzjEpas-bNcd-XB4xLx8uaVw_AhhAsCtIw9BBsMEJ1ISipL8CTlPYIEUo5eQwuMK84b6jYgOWzD_q2C0cPg4VpiQd3cB72xhtofk3RpOSCh-09_PblykHnu0WbBNUUpjkkl3IE7pZR5d1Mag7aDMMyqAi1itr5MCq4huDgMvD7zc22qGuCn4JHVg3JPDufl-Dn-3c_th-L668fPm2vrgvNGJuLinWkzTdhWVMZRZFpCK4JsbZlGneacytaTbmt8ptY0bTIVkpg1PKGiRrTS_D2xJ2WdjSdNn6OapBTdKOK9zIoJ___8W4n-3CQjHCU-5kBr06Ao_JW-V7uwxJ9tixz4wlCPC-65nl9zhPD3WLSLEeX1rqVN2FJsqppLQRhWViehDqGlKKxf7xgJNeBrlyZByodlycDL_6t4K_8PMEseHkm7oLv71z22Cp9a91gJMZN01SI0t8-Sqnz</recordid><startdate>20050207</startdate><enddate>20050207</enddate><creator>Cheng, Sheng-Quan</creator><creator>Wang, Wen-Liang</creator><creator>Yan, Wei</creator><creator>Li, Qing-Long</creator><creator>Wang, Li</creator><creator>Wang, Wen-Yong</creator><general>Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China%Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20050207</creationdate><title>Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721</title><author>Cheng, Sheng-Quan ; 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A cell model of SMMC-7721 after treatment with RNAi was prepared by transfecting SMMC-7721 cells with the lipofectin transfection method. Strept-avidin-biotin-complex (SABC) immunohistochemical staining and RT-PCR were used to detect survivin gene expressions in SMMC-7721 cells. Flow cytometry was used for the cell cycle analysis. Transmission electron microscopy was performed to determine whether RNAi induced cell apoptosis, and the method of measuring the cell growth curve was utilized to study the growth of SMMC-7721 cells before and after treatment with RNAi.RESULTS: The eukaryotic expression vector of survivin gene RNAi and pSuNeo-SW, were constructed successfully. The expression level of survivin gene in SMMC-7721 cells was observed. After the treatment of RNAi, the expression of survivin gene in SMMC-7721 cells was almost absent, apoptosis index was increased by 15.6%, and the number of cells was decreased in G2/M phase and the cell growth was inhibited.CONCLUSION: RNAi can exert a knockdown of survivin gene expression in SMMC-7721 cells, and induce apoptosis and inhibit the growth of carcinoma cells.</abstract><cop>United States</cop><pub>Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China%Department of Pathology, Faculty of Preclinical Medicine, Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China</pub><pmid>15655839</pmid><doi>10.3748/wjg.v11.i5.756</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Brief Reports Carcinoma, Hepatocellular - physiopathology Carcinoma, Hepatocellular - therapy Cell Division Cell Line, Tumor - physiology Cell Line, Tumor - ultrastructure Flow Cytometry Gene Expression Genetic Therapy - methods Humans In Vitro Techniques Inhibitor of Apoptosis Proteins Liver Neoplasms - physiopathology Liver Neoplasms - therapy Microscopy, Electron, Transmission Microtubule-Associated Proteins - genetics Neoplasm Proteins Restriction Mapping RNA, Small Interfering RNAi SMMC-7721 Survivin 基因表达 抑制作用 细胞凋亡 肝细胞癌 肿瘤细胞系统 诱导作用 |
| title | Knockdown of survivin gene expression by RNAi induces apoptosis in human hepatocellular carcinoma cell line SMMC-7721 |
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