Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis
AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonudear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divide...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2005-02, Vol.11 (5), p.661-664 |
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| creator | Gao, Hong-Kai Zhou, Zong-Guang Han, Fang-Hai Chen, You-Qin Yan, Wen-Wei He, Tao Wang, Cun Wang, Zhao |
| description | AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonudear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divided into control group (n=10) and AEP group (n=40). A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1 mRNA expression was seen from 4 to 8h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21%), while in pancreatic microcirculation, expression decreased from 2h and reached the lowest level at 6h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%, 0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral drculation and pancreatic microcirculation (1.00±0.21% vs 0.66±0.20%, P |
| doi_str_mv | 10.3748/wjg.v11.i5.661 |
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| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4250734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>11888581</cqvip_id><wanfj_id>wjg200505009</wanfj_id><sourcerecordid>wjg200505009</sourcerecordid><originalsourceid>FETCH-LOGICAL-c444t-2d9697c39e96b9535ddb891c6500f61b5e40cc0fc756641c588b810a8e2e70a83</originalsourceid><addsrcrecordid>eNpVkcFu1DAQhi1ERbeFK0dkIa4JthMnzgUJFVqQKvUCZ8uxJxsvjhNsp1uejNfDy65okQ8jz3zzz9g_Qq8pKau2Fu_3u215T2lpedk09BnaMEa7gomaPEcbSkhbdBVrz9FFjDtCWFVx9gKdU95wLmi7Qb8_2WGAAF5DxNbjxakEDhIGb-Y0grPKYQ3OYWVGiHb2eJod6NVBQTE8LAHi32wPaQ-QBSDYZYRwaLMhcyodysobvCivA-S7xpPVYX5az6M1hKxqfWG9WTUYrPSaAIOBSaV5jY_9ycaX6GxQLsKrU7xE368_f7v6Utze3Xy9-nhb6LquU8FM13Strjromr7jFTemFx3VDSdkaGjPoSZak0G3vGlqqrkQvaBECWDQ5lBdog9H3WXtJzAafMpPk0uwkwq_5Kys_L_i7Si3872sGSdtVWeBd0eBvfKD8lu5m9fg88oyW8cI4fmQLmPlEcv_EmOA4d8ISuTB6QMus9PScpmdzg1vni72iJ-szcDbk-I4--1Pm0f3Sv8YrANJqRAiU9UfVIi32A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis</title><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Gao, Hong-Kai ; Zhou, Zong-Guang ; Han, Fang-Hai ; Chen, You-Qin ; Yan, Wen-Wei ; He, Tao ; Wang, Cun ; Wang, Zhao</creator><creatorcontrib>Gao, Hong-Kai ; Zhou, Zong-Guang ; Han, Fang-Hai ; Chen, You-Qin ; Yan, Wen-Wei ; He, Tao ; Wang, Cun ; Wang, Zhao</creatorcontrib><description>AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonudear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divided into control group (n=10) and AEP group (n=40). A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1 mRNA expression was seen from 4 to 8h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21%), while in pancreatic microcirculation, expression decreased from 2h and reached the lowest level at 6h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%, 0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral drculation and pancreatic microcirculation (1.00±0.21% vs 0.66±0.20%, P<0.05).Meanwhile, the difference at protein level was also found.CONCLUSION: A reverse expression of PECAM-1 on PMNs was found between peripheral circulation and pancreatic microcirculation, suggesting that inhibition of PECAM-1 expression may improve the pathological change of AEP.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v11.i5.661</identifier><identifier>PMID: 15655817</identifier><language>eng</language><publisher>United States: Department of General Surgery & Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China%Department of Gastrointestinal Surgery, First Affiliated Hospital, Zhongshan University, Guangzhou 510120, Guangdong Province, China%Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA</publisher><subject>Acute Disease ; Amylases - blood ; Animals ; Basic Research ; Ceruletide ; Edema - chemically induced ; Edema - pathology ; Edema - physiopathology ; Flow Cytometry ; Gene Expression ; Male ; Microcirculation ; Neutrophils - physiology ; Organ Size ; Pancreas - blood supply ; Pancreatitis - chemically induced ; Pancreatitis - pathology ; Pancreatitis - physiopathology ; Platelet Endothelial Cell Adhesion Molecule-1 - genetics ; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; 内皮细胞 ; 基因表达 ; 外周循环 ; 急性胰腺炎水肿 ; 支持分子-1 ; 消化系统 ; 胰腺微循环 ; 蛙皮缩胆囊肽 ; 血小板 ; 诱导作用</subject><ispartof>World journal of gastroenterology : WJG, 2005-02, Vol.11 (5), p.661-664</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2005 Baishideng Publishing Group Inc. All rights reserved. 2005</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-2d9697c39e96b9535ddb891c6500f61b5e40cc0fc756641c588b810a8e2e70a83</citedby><cites>FETCH-LOGICAL-c444t-2d9697c39e96b9535ddb891c6500f61b5e40cc0fc756641c588b810a8e2e70a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250734/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4250734/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15655817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Hong-Kai</creatorcontrib><creatorcontrib>Zhou, Zong-Guang</creatorcontrib><creatorcontrib>Han, Fang-Hai</creatorcontrib><creatorcontrib>Chen, You-Qin</creatorcontrib><creatorcontrib>Yan, Wen-Wei</creatorcontrib><creatorcontrib>He, Tao</creatorcontrib><creatorcontrib>Wang, Cun</creatorcontrib><creatorcontrib>Wang, Zhao</creatorcontrib><title>Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM: To investigate the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonudear leukocytes (PMNs) in peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis (AEP).METHODS: Fifty Wistar rats were randomly divided into control group (n=10) and AEP group (n=40). A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1 mRNA expression was seen from 4 to 8h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21%), while in pancreatic microcirculation, expression decreased from 2h and reached the lowest level at 6h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%, 0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral drculation and pancreatic microcirculation (1.00±0.21% vs 0.66±0.20%, P<0.05).Meanwhile, the difference at protein level was also found.CONCLUSION: A reverse expression of PECAM-1 on PMNs was found between peripheral circulation and pancreatic microcirculation, suggesting that inhibition of PECAM-1 expression may improve the pathological change of AEP.</description><subject>Acute Disease</subject><subject>Amylases - blood</subject><subject>Animals</subject><subject>Basic Research</subject><subject>Ceruletide</subject><subject>Edema - chemically induced</subject><subject>Edema - pathology</subject><subject>Edema - physiopathology</subject><subject>Flow Cytometry</subject><subject>Gene Expression</subject><subject>Male</subject><subject>Microcirculation</subject><subject>Neutrophils - physiology</subject><subject>Organ Size</subject><subject>Pancreas - blood supply</subject><subject>Pancreatitis - chemically induced</subject><subject>Pancreatitis - pathology</subject><subject>Pancreatitis - physiopathology</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - genetics</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>内皮细胞</subject><subject>基因表达</subject><subject>外周循环</subject><subject>急性胰腺炎水肿</subject><subject>支持分子-1</subject><subject>消化系统</subject><subject>胰腺微循环</subject><subject>蛙皮缩胆囊肽</subject><subject>血小板</subject><subject>诱导作用</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFu1DAQhi1ERbeFK0dkIa4JthMnzgUJFVqQKvUCZ8uxJxsvjhNsp1uejNfDy65okQ8jz3zzz9g_Qq8pKau2Fu_3u215T2lpedk09BnaMEa7gomaPEcbSkhbdBVrz9FFjDtCWFVx9gKdU95wLmi7Qb8_2WGAAF5DxNbjxakEDhIGb-Y0grPKYQ3OYWVGiHb2eJod6NVBQTE8LAHi32wPaQ-QBSDYZYRwaLMhcyodysobvCivA-S7xpPVYX5az6M1hKxqfWG9WTUYrPSaAIOBSaV5jY_9ycaX6GxQLsKrU7xE368_f7v6Utze3Xy9-nhb6LquU8FM13Strjromr7jFTemFx3VDSdkaGjPoSZak0G3vGlqqrkQvaBECWDQ5lBdog9H3WXtJzAafMpPk0uwkwq_5Kys_L_i7Si3872sGSdtVWeBd0eBvfKD8lu5m9fg88oyW8cI4fmQLmPlEcv_EmOA4d8ISuTB6QMus9PScpmdzg1vni72iJ-szcDbk-I4--1Pm0f3Sv8YrANJqRAiU9UfVIi32A</recordid><startdate>20050207</startdate><enddate>20050207</enddate><creator>Gao, Hong-Kai</creator><creator>Zhou, Zong-Guang</creator><creator>Han, Fang-Hai</creator><creator>Chen, You-Qin</creator><creator>Yan, Wen-Wei</creator><creator>He, Tao</creator><creator>Wang, Cun</creator><creator>Wang, Zhao</creator><general>Department of General Surgery & Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China%Department of Gastrointestinal Surgery, First Affiliated Hospital, Zhongshan University, Guangzhou 510120, Guangdong Province, China%Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20050207</creationdate><title>Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis</title><author>Gao, Hong-Kai ; 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A model of AEP was established by subcutaneous injection of cerulein 5.5 and 7.5μg/kg at 0 and 1 h after the beginning of experiment respectively. PECAM-1 expression on PMNs from splenic vein and inferior vena cava was determined by RT-PCR at mRNA level and determined by flow cytometry at protein level.RESULTS: In experimental rats, an increased PECAM-1 mRNA expression was seen from 4 to 8h of AEP in peripheral circulation (0.77±0.25%, 0.76±0.28%, 0.89±0.30%,1.00±0.21%), while in pancreatic microcirculation, expression decreased from 2h and reached the lowest level at 6h of AEP (0.78±0.29%, 0.75±0.26%, 0.62±0.28%, 0.66±0.20%). There were significant differences at 8-h time point of AEP between peripheral drculation and pancreatic microcirculation (1.00±0.21% vs 0.66±0.20%, P<0.05).Meanwhile, the difference at protein level was also found.CONCLUSION: A reverse expression of PECAM-1 on PMNs was found between peripheral circulation and pancreatic microcirculation, suggesting that inhibition of PECAM-1 expression may improve the pathological change of AEP.</abstract><cop>United States</cop><pub>Department of General Surgery & Division of Digestive Surgery and Organ-Microcirculation, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China%Department of Gastrointestinal Surgery, First Affiliated Hospital, Zhongshan University, Guangzhou 510120, Guangdong Province, China%Cardiovascular Biology, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA</pub><pmid>15655817</pmid><doi>10.3748/wjg.v11.i5.661</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2005-02, Vol.11 (5), p.661-664 |
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| subjects | Acute Disease Amylases - blood Animals Basic Research Ceruletide Edema - chemically induced Edema - pathology Edema - physiopathology Flow Cytometry Gene Expression Male Microcirculation Neutrophils - physiology Organ Size Pancreas - blood supply Pancreatitis - chemically induced Pancreatitis - pathology Pancreatitis - physiopathology Platelet Endothelial Cell Adhesion Molecule-1 - genetics Platelet Endothelial Cell Adhesion Molecule-1 - metabolism Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction 内皮细胞 基因表达 外周循环 急性胰腺炎水肿 支持分子-1 消化系统 胰腺微循环 蛙皮缩胆囊肽 血小板 诱导作用 |
| title | Differences in platelet endothelial cell adhesion molecule-1 expression between peripheral circulation and pancreatic microcirculation in cerulein-induced acute edematous pancreatitis |
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