New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712
Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2014-12, Vol.58 (12), p.7441-7450 |
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| creator | Fernández-Martínez, Lorena T Borsetto, Chiara Gomez-Escribano, Juan Pablo Bibb, Maureen J Al-Bassam, Mahmoud M Chandra, Govind Bibb, Mervyn J |
| description | Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway. |
| doi_str_mv | 10.1128/AAC.04272-14 |
| format | Article |
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Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.</description><identifier>ISSN: 0066-4804</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AAC.04272-14</identifier><identifier>PMID: 25267678</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Acyl Carrier Protein - genetics ; Acyl Carrier Protein - metabolism ; Bacterial Proteins ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biosynthesis ; Chemistry ; Chemistry; Biosynthesis ; Chloramphenicol ; Chloramphenicol - biosynthesis ; Gene Deletion ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Metabolic Networks and Pathways ; Metabolic Networks and Pathways - genetics ; Microarray Analysis ; Molecular Sequence Annotation ; Multigene Family ; Mutation ; Sequence Analysis, DNA ; Sodium-Hydrogen Exchangers - genetics ; Sodium-Hydrogen Exchangers - metabolism ; Streptomyces ; Streptomyces - genetics ; Streptomyces - metabolism ; Streptomyces venezuelae ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Transferases (Other Substituted Phosphate Groups) - genetics ; Transferases (Other Substituted Phosphate Groups) - metabolism</subject><ispartof>Antimicrobial agents and chemotherapy, 2014-12, Vol.58 (12), p.7441-7450</ispartof><rights>Copyright © 2014 Fernández-Martínez et al.</rights><rights>Copyright © 2014 Fernández-Martínez et al. 2014 Fernández-Martínez et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a494t-c2ed3a395a00926f8e049ccb0b70439ae91e93e228f4201741d539143a92940e3</citedby><cites>FETCH-LOGICAL-a494t-c2ed3a395a00926f8e049ccb0b70439ae91e93e228f4201741d539143a92940e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249514/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249514/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25267678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fernández-Martínez, Lorena T</creatorcontrib><creatorcontrib>Borsetto, Chiara</creatorcontrib><creatorcontrib>Gomez-Escribano, Juan Pablo</creatorcontrib><creatorcontrib>Bibb, Maureen J</creatorcontrib><creatorcontrib>Al-Bassam, Mahmoud M</creatorcontrib><creatorcontrib>Chandra, Govind</creatorcontrib><creatorcontrib>Bibb, Mervyn J</creatorcontrib><title>New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.</description><subject>Acyl Carrier Protein - genetics</subject><subject>Acyl Carrier Protein - metabolism</subject><subject>Bacterial Proteins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biosynthesis</subject><subject>Chemistry</subject><subject>Chemistry; Biosynthesis</subject><subject>Chloramphenicol</subject><subject>Chloramphenicol - biosynthesis</subject><subject>Gene Deletion</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Metabolic Networks and Pathways</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>Microarray Analysis</subject><subject>Molecular Sequence Annotation</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium-Hydrogen Exchangers - genetics</subject><subject>Sodium-Hydrogen Exchangers - metabolism</subject><subject>Streptomyces</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces venezuelae</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transferases (Other Substituted Phosphate Groups) - genetics</subject><subject>Transferases (Other Substituted Phosphate Groups) - metabolism</subject><issn>0066-4804</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kT1PwzAQhi0EglLYmFFGkAicHceJF6Qq4ktCMFBmy3WvxCiJi50UlV9PSqGCgcln3aPndPcSckThnFKWX4xGxTlwlrGY8i0yoCDzWKRSbJMBgBAxz4Hvkf0QXqH_pxJ2yR5LmchElg_I-AHfI9sE-1K2oS9aF5mycl7X8xIba1wVTawLy6YtMdgVET21Huetq5cGQ7TABj86rDRGo3FRRBQyyg7IzkxXAQ-_3yF5vr4aF7fx_ePNXTG6jzWXvI0Nw2miE5lqAMnELEfg0pgJTDLgidQoKcoEGctnnAHNOJ2miaQ80ZJJDpgMyeXaO-8mNU4NNq3XlZp7W2u_VE5b9bfT2FK9uIXijMu0Fw3JybfAu7cOQ6tqGwxWlW7QdUFRwZmgHFjao2dr1HgXgsfZZgwFtQpC9UGoryDUl_l0jetQM_XqOt_0l_iPPf69xkb8k1LyCXk1j7c</recordid><startdate>20141201</startdate><enddate>20141201</enddate><creator>Fernández-Martínez, Lorena T</creator><creator>Borsetto, Chiara</creator><creator>Gomez-Escribano, Juan Pablo</creator><creator>Bibb, Maureen J</creator><creator>Al-Bassam, Mahmoud M</creator><creator>Chandra, Govind</creator><creator>Bibb, Mervyn J</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20141201</creationdate><title>New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712</title><author>Fernández-Martínez, Lorena T ; Borsetto, Chiara ; Gomez-Escribano, Juan Pablo ; Bibb, Maureen J ; Al-Bassam, Mahmoud M ; Chandra, Govind ; Bibb, Mervyn J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a494t-c2ed3a395a00926f8e049ccb0b70439ae91e93e228f4201741d539143a92940e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acyl Carrier Protein - genetics</topic><topic>Acyl Carrier Protein - metabolism</topic><topic>Bacterial Proteins</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biosynthesis</topic><topic>Chemistry</topic><topic>Chemistry; Biosynthesis</topic><topic>Chloramphenicol</topic><topic>Chloramphenicol - biosynthesis</topic><topic>Gene Deletion</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Metabolic Networks and Pathways</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>Microarray Analysis</topic><topic>Molecular Sequence Annotation</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium-Hydrogen Exchangers - genetics</topic><topic>Sodium-Hydrogen Exchangers - metabolism</topic><topic>Streptomyces</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces venezuelae</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transferases (Other Substituted Phosphate Groups) - genetics</topic><topic>Transferases (Other Substituted Phosphate Groups) - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fernández-Martínez, Lorena T</creatorcontrib><creatorcontrib>Borsetto, Chiara</creatorcontrib><creatorcontrib>Gomez-Escribano, Juan Pablo</creatorcontrib><creatorcontrib>Bibb, Maureen J</creatorcontrib><creatorcontrib>Al-Bassam, Mahmoud M</creatorcontrib><creatorcontrib>Chandra, Govind</creatorcontrib><creatorcontrib>Bibb, Mervyn J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial agents and chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fernández-Martínez, Lorena T</au><au>Borsetto, Chiara</au><au>Gomez-Escribano, Juan Pablo</au><au>Bibb, Maureen J</au><au>Al-Bassam, Mahmoud M</au><au>Chandra, Govind</au><au>Bibb, Mervyn J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712</atitle><jtitle>Antimicrobial agents and chemotherapy</jtitle><stitle>Antimicrob Agents Chemother</stitle><addtitle>Antimicrob Agents Chemother</addtitle><date>2014-12-01</date><risdate>2014</risdate><volume>58</volume><issue>12</issue><spage>7441</spage><epage>7450</epage><pages>7441-7450</pages><issn>0066-4804</issn><eissn>1098-6596</eissn><abstract>Comparative genome analysis revealed seven uncharacterized genes, sven0909 to sven0915, adjacent to the previously identified chloramphenicol biosynthetic gene cluster (sven0916-sven0928) of Streptomyces venezuelae strain ATCC 10712 that was absent in a closely related Streptomyces strain that does not produce chloramphenicol. Transcriptional analysis suggested that three of these genes might be involved in chloramphenicol production, a prediction confirmed by the construction of deletion mutants. These three genes encode a cluster-associated transcriptional activator (Sven0913), a phosphopantetheinyl transferase (Sven0914), and a Na(+)/H(+) antiporter (Sven0915). Bioinformatic analysis also revealed the presence of a previously undetected gene, sven0925, embedded within the chloramphenicol biosynthetic gene cluster that appears to encode an acyl carrier protein, bringing the number of new genes likely to be involved in chloramphenicol production to four. Microarray experiments and synteny comparisons also suggest that sven0929 is part of the biosynthetic gene cluster. This has allowed us to propose an updated and revised version of the chloramphenicol biosynthetic pathway.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>25267678</pmid><doi>10.1128/AAC.04272-14</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Antimicrobial agents and chemotherapy, 2014-12, Vol.58 (12), p.7441-7450 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Acyl Carrier Protein - genetics Acyl Carrier Protein - metabolism Bacterial Proteins Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosynthesis Chemistry Chemistry Biosynthesis Chloramphenicol Chloramphenicol - biosynthesis Gene Deletion Gene Expression Profiling Gene Expression Regulation, Bacterial Metabolic Networks and Pathways Metabolic Networks and Pathways - genetics Microarray Analysis Molecular Sequence Annotation Multigene Family Mutation Sequence Analysis, DNA Sodium-Hydrogen Exchangers - genetics Sodium-Hydrogen Exchangers - metabolism Streptomyces Streptomyces - genetics Streptomyces - metabolism Streptomyces venezuelae Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Transferases (Other Substituted Phosphate Groups) - genetics Transferases (Other Substituted Phosphate Groups) - metabolism |
| title | New insights into chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712 |
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