Changes in ion fluxes during phototropic bending of etiolated oat coleoptiles
Background and Aims This work has been conducted to assist theoretical modelling of the different stages of the blue light (BL)-induced phototropic signalling pathway and ion transport activity across plant membranes. Ion fluxes (Ca2+, H+, K+ and Cl-) in etiolated oat coleoptiles have been measured...
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| Veröffentlicht in: | Annals of botany 2004-07, Vol.94 (1), p.187-194 |
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| description | Background and Aims This work has been conducted to assist theoretical modelling of the different stages of the blue light (BL)-induced phototropic signalling pathway and ion transport activity across plant membranes. Ion fluxes (Ca2+, H+, K+ and Cl-) in etiolated oat coleoptiles have been measured continuously before and during unilateral BL exposure. Methods Changes in ion fluxes at the illuminated (light) and shadowed (dark) sides of etiolated oat coleoptiles (Avena sativa) were studied using a non-invasive ion-selective microelectrode technique (MIFE). The bending response was also measured continuously, and correlations between the changes in various ion fluxes and bending response have been investigated. For each ion the difference (delta) between the magnitudes of flux at the light and dark sides of the coleoptile was calculated. Key results Plants that demonstrated a phototropic bending response also demonstrated Ca2+ influx into the light side approximately 20 min after the start of BL exposure. This is regarded as part of the perception and transduction stages of the BL-induced signal cascade. The first 10 min of bending were associated with substantial influx of H+, K+ and Cl- into the light (concave) side of the coleoptiles. Conclusions The data suggest that Ca2+ participates in the signalling stage of the BL-induced phototropism, whereas the phototropic bending response is linked to changes in the transport of H+, K+ and Cl-. |
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Ion fluxes (Ca2+, H+, K+ and Cl-) in etiolated oat coleoptiles have been measured continuously before and during unilateral BL exposure. Methods Changes in ion fluxes at the illuminated (light) and shadowed (dark) sides of etiolated oat coleoptiles (Avena sativa) were studied using a non-invasive ion-selective microelectrode technique (MIFE). The bending response was also measured continuously, and correlations between the changes in various ion fluxes and bending response have been investigated. For each ion the difference (delta) between the magnitudes of flux at the light and dark sides of the coleoptile was calculated. Key results Plants that demonstrated a phototropic bending response also demonstrated Ca2+ influx into the light side approximately 20 min after the start of BL exposure. This is regarded as part of the perception and transduction stages of the BL-induced signal cascade. The first 10 min of bending were associated with substantial influx of H+, K+ and Cl- into the light (concave) side of the coleoptiles. Conclusions The data suggest that Ca2+ participates in the signalling stage of the BL-induced phototropism, whereas the phototropic bending response is linked to changes in the transport of H+, K+ and Cl-.</description><identifier>ISSN: 0305-7364</identifier><identifier>EISSN: 1095-8290</identifier><identifier>DOI: 10.1093/aob/mch126</identifier><identifier>PMID: 15155378</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Auxins ; Avena - growth & development ; Avena - metabolism ; Avena - radiation effects ; Avena sativa ; Bending ; blue light ; Ca2 ; calcium ; Calcium - metabolism ; Cell Membrane - metabolism ; cell membranes ; chlorides ; Chlorine - metabolism ; Cl ; Coleoptiles ; Cotyledon - growth & development ; Cotyledon - metabolism ; Cotyledon - radiation effects ; Darkness ; Electrodes ; etiolation ; hydrogen ; Hydrogen - metabolism ; ion fluxes ; ion transport ; Ions ; Light ; membrane transport ; Models, Theoretical ; Oats ; Original ; Phototropism ; Plant cells ; Plants ; potassium ; Potassium - metabolism ; Seedlings ; Signal Transduction ; Time Factors</subject><ispartof>Annals of botany, 2004-07, Vol.94 (1), p.187-194</ispartof><rights>Annals of Botany Company 2004</rights><rights>Copyright Oxford University Press(England) Jul 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-24d2ca9beaaaa508b255f9b9c5c181fb4fe292c7756b6d3cefa38684fb1a00223</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42759189$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42759189$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27903,27904,53770,53772,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15155378$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Babourina, O</creatorcontrib><creatorcontrib>Godfrey, L</creatorcontrib><creatorcontrib>Voltchanskii, K</creatorcontrib><title>Changes in ion fluxes during phototropic bending of etiolated oat coleoptiles</title><title>Annals of botany</title><addtitle>Ann Bot</addtitle><description>Background and Aims This work has been conducted to assist theoretical modelling of the different stages of the blue light (BL)-induced phototropic signalling pathway and ion transport activity across plant membranes. Ion fluxes (Ca2+, H+, K+ and Cl-) in etiolated oat coleoptiles have been measured continuously before and during unilateral BL exposure. Methods Changes in ion fluxes at the illuminated (light) and shadowed (dark) sides of etiolated oat coleoptiles (Avena sativa) were studied using a non-invasive ion-selective microelectrode technique (MIFE). The bending response was also measured continuously, and correlations between the changes in various ion fluxes and bending response have been investigated. For each ion the difference (delta) between the magnitudes of flux at the light and dark sides of the coleoptile was calculated. Key results Plants that demonstrated a phototropic bending response also demonstrated Ca2+ influx into the light side approximately 20 min after the start of BL exposure. This is regarded as part of the perception and transduction stages of the BL-induced signal cascade. The first 10 min of bending were associated with substantial influx of H+, K+ and Cl- into the light (concave) side of the coleoptiles. Conclusions The data suggest that Ca2+ participates in the signalling stage of the BL-induced phototropism, whereas the phototropic bending response is linked to changes in the transport of H+, K+ and Cl-.</description><subject>Auxins</subject><subject>Avena - growth & development</subject><subject>Avena - metabolism</subject><subject>Avena - radiation effects</subject><subject>Avena sativa</subject><subject>Bending</subject><subject>blue light</subject><subject>Ca2</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>cell membranes</subject><subject>chlorides</subject><subject>Chlorine - metabolism</subject><subject>Cl</subject><subject>Coleoptiles</subject><subject>Cotyledon - growth & development</subject><subject>Cotyledon - metabolism</subject><subject>Cotyledon - radiation effects</subject><subject>Darkness</subject><subject>Electrodes</subject><subject>etiolation</subject><subject>hydrogen</subject><subject>Hydrogen - metabolism</subject><subject>ion fluxes</subject><subject>ion transport</subject><subject>Ions</subject><subject>Light</subject><subject>membrane transport</subject><subject>Models, Theoretical</subject><subject>Oats</subject><subject>Original</subject><subject>Phototropism</subject><subject>Plant cells</subject><subject>Plants</subject><subject>potassium</subject><subject>Potassium - metabolism</subject><subject>Seedlings</subject><subject>Signal Transduction</subject><subject>Time Factors</subject><issn>0305-7364</issn><issn>1095-8290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1vEzEQxS0Eomnhwh1YcURa6s9d-1IJRbQBGnGASoiLZXvtxGGzXmxvVf57HG0UwJeR5_30ZuYB8ALBdwgKcqmCvtybLcLNI7AoHVZzLOBjsIAEsrolDT0D5yntIIS4EegpOEMMMUZavgDr5VYNG5sqP1Q-DJXrp4fy66boh001bkMOOYbRm0rboTv0gqts9qFX2XZVULkyobdhzL636Rl44lSf7PNjvQB31x--LVf17Zebj8v3t7WhnOUa0w4bJbRV5THINWbMCS0MM4gjp6mzWGDTtqzRTUeMdYrwhlOnkSo3YHIBrmbfcdJ72xk75Kh6OUa_V_G3DMrL_5XBb-Um3EuKKSb8YPDmaBDDr8mmLHdhikPZWSLBSoRUiAK9nSETQ0rRutMABOUheVmSl3PyBX7170p_0WPUBXg5A7uUQzzpFLdMIH6YVs-6T9k-nHQVf8qmJS2Tq-8_5Ppmtb7-zFbyU-Ffz7xTQapN9EnefcUQEQgFxYK15A-TnqPl</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Babourina, O</creator><creator>Godfrey, L</creator><creator>Voltchanskii, K</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7T7</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20040701</creationdate><title>Changes in ion fluxes during phototropic bending of etiolated oat coleoptiles</title><author>Babourina, O ; Godfrey, L ; Voltchanskii, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-24d2ca9beaaaa508b255f9b9c5c181fb4fe292c7756b6d3cefa38684fb1a00223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Auxins</topic><topic>Avena - growth & development</topic><topic>Avena - metabolism</topic><topic>Avena - radiation effects</topic><topic>Avena sativa</topic><topic>Bending</topic><topic>blue light</topic><topic>Ca2</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>cell membranes</topic><topic>chlorides</topic><topic>Chlorine - metabolism</topic><topic>Cl</topic><topic>Coleoptiles</topic><topic>Cotyledon - growth & development</topic><topic>Cotyledon - metabolism</topic><topic>Cotyledon - radiation effects</topic><topic>Darkness</topic><topic>Electrodes</topic><topic>etiolation</topic><topic>hydrogen</topic><topic>Hydrogen - metabolism</topic><topic>ion fluxes</topic><topic>ion transport</topic><topic>Ions</topic><topic>Light</topic><topic>membrane transport</topic><topic>Models, Theoretical</topic><topic>Oats</topic><topic>Original</topic><topic>Phototropism</topic><topic>Plant cells</topic><topic>Plants</topic><topic>potassium</topic><topic>Potassium - metabolism</topic><topic>Seedlings</topic><topic>Signal Transduction</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Babourina, O</creatorcontrib><creatorcontrib>Godfrey, L</creatorcontrib><creatorcontrib>Voltchanskii, K</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Annals of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Babourina, O</au><au>Godfrey, L</au><au>Voltchanskii, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes in ion fluxes during phototropic bending of etiolated oat coleoptiles</atitle><jtitle>Annals of botany</jtitle><addtitle>Ann Bot</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>94</volume><issue>1</issue><spage>187</spage><epage>194</epage><pages>187-194</pages><issn>0305-7364</issn><eissn>1095-8290</eissn><abstract>Background and Aims This work has been conducted to assist theoretical modelling of the different stages of the blue light (BL)-induced phototropic signalling pathway and ion transport activity across plant membranes. Ion fluxes (Ca2+, H+, K+ and Cl-) in etiolated oat coleoptiles have been measured continuously before and during unilateral BL exposure. Methods Changes in ion fluxes at the illuminated (light) and shadowed (dark) sides of etiolated oat coleoptiles (Avena sativa) were studied using a non-invasive ion-selective microelectrode technique (MIFE). The bending response was also measured continuously, and correlations between the changes in various ion fluxes and bending response have been investigated. For each ion the difference (delta) between the magnitudes of flux at the light and dark sides of the coleoptile was calculated. Key results Plants that demonstrated a phototropic bending response also demonstrated Ca2+ influx into the light side approximately 20 min after the start of BL exposure. This is regarded as part of the perception and transduction stages of the BL-induced signal cascade. The first 10 min of bending were associated with substantial influx of H+, K+ and Cl- into the light (concave) side of the coleoptiles. Conclusions The data suggest that Ca2+ participates in the signalling stage of the BL-induced phototropism, whereas the phototropic bending response is linked to changes in the transport of H+, K+ and Cl-.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>15155378</pmid><doi>10.1093/aob/mch126</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Auxins Avena - growth & development Avena - metabolism Avena - radiation effects Avena sativa Bending blue light Ca2 calcium Calcium - metabolism Cell Membrane - metabolism cell membranes chlorides Chlorine - metabolism Cl Coleoptiles Cotyledon - growth & development Cotyledon - metabolism Cotyledon - radiation effects Darkness Electrodes etiolation hydrogen Hydrogen - metabolism ion fluxes ion transport Ions Light membrane transport Models, Theoretical Oats Original Phototropism Plant cells Plants potassium Potassium - metabolism Seedlings Signal Transduction Time Factors |
| title | Changes in ion fluxes during phototropic bending of etiolated oat coleoptiles |
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