DNA methylation profiles provide a viable index for porcine pluripotent stem cells
Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2013-11, Vol.51 (11), p.763-776 |
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| creator | Arai, Yoshikazu Ohgane, Jun Fujishiro, Shuh-hei Nakano, Kazuaki Matsunari, Hitomi Watanabe, Masahito Umeyama, Kazuhiro Azuma, Dai Uchida, Naomi Sakamoto, Nozomu Makino, Tomohiro Yagi, Shintaro Shiota, Kunio Hanazono, Yutaka Nagashima, Hiroshi |
| description | Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)‐specific hypomethylated loci (EShypo‐T‐DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso‐4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso‐4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso‐622‐14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum‐free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/dvg.22423 |
| format | Article |
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The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)‐specific hypomethylated loci (EShypo‐T‐DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso‐4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso‐4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso‐622‐14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum‐free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc.</description><identifier>ISSN: 1526-954X</identifier><identifier>EISSN: 1526-968X</identifier><identifier>DOI: 10.1002/dvg.22423</identifier><identifier>PMID: 23913699</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Blastocyst Inner Cell Mass - metabolism ; Cell Line ; Chimera ; DNA Methylation ; Embryo, Mammalian ; Embryonic Stem Cells - metabolism ; epigenetics ; Gene Expression ; Genes ; Genetic Loci ; induced pluripotent stem cells ; Induced Pluripotent Stem Cells - metabolism ; Mice ; Swine - embryology ; Swine, Miniature - embryology ; Translational Medical Research ; translational research</subject><ispartof>Genesis (New York, N.Y. : 2000), 2013-11, Vol.51 (11), p.763-776</ispartof><rights>Copyright © 2013 The Authors. Genesis Published by Wiley Periodicals, Inc.</rights><rights>Copyright © 2013 Wiley Periodicals, Inc.</rights><rights>Copyright © 2013 Wiley Periodicals, Inc. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5803-6985a43bfd8b5c75b7219204403bb64235fc46098f8a53dc07ccda67871b8dca3</citedby><cites>FETCH-LOGICAL-c5803-6985a43bfd8b5c75b7219204403bb64235fc46098f8a53dc07ccda67871b8dca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdvg.22423$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdvg.22423$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23913699$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arai, Yoshikazu</creatorcontrib><creatorcontrib>Ohgane, Jun</creatorcontrib><creatorcontrib>Fujishiro, Shuh-hei</creatorcontrib><creatorcontrib>Nakano, Kazuaki</creatorcontrib><creatorcontrib>Matsunari, Hitomi</creatorcontrib><creatorcontrib>Watanabe, Masahito</creatorcontrib><creatorcontrib>Umeyama, Kazuhiro</creatorcontrib><creatorcontrib>Azuma, Dai</creatorcontrib><creatorcontrib>Uchida, Naomi</creatorcontrib><creatorcontrib>Sakamoto, Nozomu</creatorcontrib><creatorcontrib>Makino, Tomohiro</creatorcontrib><creatorcontrib>Yagi, Shintaro</creatorcontrib><creatorcontrib>Shiota, Kunio</creatorcontrib><creatorcontrib>Hanazono, Yutaka</creatorcontrib><creatorcontrib>Nagashima, Hiroshi</creatorcontrib><title>DNA methylation profiles provide a viable index for porcine pluripotent stem cells</title><title>Genesis (New York, N.Y. : 2000)</title><addtitle>genesis</addtitle><description>Porcine induced pluripotent stem cells (iPSCs) provide useful information for translational research. The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)‐specific hypomethylated loci (EShypo‐T‐DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso‐4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso‐4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso‐622‐14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum‐free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>Blastocyst Inner Cell Mass - metabolism</subject><subject>Cell Line</subject><subject>Chimera</subject><subject>DNA Methylation</subject><subject>Embryo, Mammalian</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>epigenetics</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Genetic Loci</subject><subject>induced pluripotent stem cells</subject><subject>Induced Pluripotent Stem Cells - metabolism</subject><subject>Mice</subject><subject>Swine - embryology</subject><subject>Swine, Miniature - embryology</subject><subject>Translational Medical Research</subject><subject>translational research</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EoqVw4A8gS1zgkNYf8dcFqezCgqiKBAVWXCzHcVqXJA52snT_PV62uwIkxMkj-ZlHM_MC8BijY4wQOalXl8eElITeAYeYEV4oLpd3dzUrlwfgQUrXCCEmCbkPDghVmHKlDsGH-fkp7Nx4tW7N6EMPhxga37q0KVa-dtDAlTdV66Dva3cDmxDhEKL1vYNDO0U_hNH1I0yj66B1bZsegnuNaZN7dPsegU-vX13M3hRn7xdvZ6dnhWUS0YIryUxJq6aWFbOCVYJgRVBZIlpVPC_DGltypGQjDaO1RcLa2nAhBa5kbQ09Ai-23mGqOlfbPEU0rR6i70xc62C8_vOn91f6Mqx0dgvMcBY8uxXE8H1yadSdT5sVTO_ClDTmXBKBlGL_R0uOpWRUbqxP_0KvwxT7fAmNBS1pTozyTD3fUjaGlKJr9nNjpDeh6hyq_hVqZp_8vuie3KWYgZMt8CMnt_63Sc8_L3bKYtvhc243-w4Tv2kuqGD6y_lCzy5efp1_XAr9jv4EL4W6_A</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>Arai, Yoshikazu</creator><creator>Ohgane, Jun</creator><creator>Fujishiro, Shuh-hei</creator><creator>Nakano, Kazuaki</creator><creator>Matsunari, Hitomi</creator><creator>Watanabe, Masahito</creator><creator>Umeyama, Kazuhiro</creator><creator>Azuma, Dai</creator><creator>Uchida, Naomi</creator><creator>Sakamoto, Nozomu</creator><creator>Makino, Tomohiro</creator><creator>Yagi, Shintaro</creator><creator>Shiota, Kunio</creator><creator>Hanazono, Yutaka</creator><creator>Nagashima, Hiroshi</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><general>BlackWell Publishing Ltd</general><scope>BSCLL</scope><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201311</creationdate><title>DNA methylation profiles provide a viable index for porcine pluripotent stem cells</title><author>Arai, Yoshikazu ; 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The quality of iPSCs can be assessed by their ability to differentiate into various cell types after chimera formation. However, analysis of chimera formation in pigs is a labor‐intensive and costly process, necessitating a simple evaluation method for porcine iPSCs. Our previous study identified mouse embryonic stem cell (ESC)‐specific hypomethylated loci (EShypo‐T‐DMRs), and, in this study, 36 genes selected from these were used to evaluate porcine iPSC lines. Based on the methylation profiles of the 36 genes, the iPSC line, Porco Rosso‐4, was found closest to mouse pluripotent stem cells among 5 porcine iPSCs. Moreover, Porco Rosso‐4 more efficiently contributed to the inner cell mass (ICM) of blastocysts than the iPSC line showing the lowest reprogramming of the 36 genes (Porco Rosso‐622‐14), indicating that the DNA methylation profile correlates with efficiency of ICM contribution. Furthermore, factors known to enhance iPSC quality (serum‐free medium with PD0325901 and CHIR99021) improved the methylation status at the 36 genes. Thus, the DNA methylation profile of these 36 genes is a viable index for evaluation of porcine iPSCs. genesis 51:763–776. © 2013 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>23913699</pmid><doi>10.1002/dvg.22423</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Blastocyst Inner Cell Mass - metabolism Cell Line Chimera DNA Methylation Embryo, Mammalian Embryonic Stem Cells - metabolism epigenetics Gene Expression Genes Genetic Loci induced pluripotent stem cells Induced Pluripotent Stem Cells - metabolism Mice Swine - embryology Swine, Miniature - embryology Translational Medical Research translational research |
| title | DNA methylation profiles provide a viable index for porcine pluripotent stem cells |
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