Comparison of biological properties of umbilical cord-derived mesenchymal stem cells from early and late passages: Immunomodulatory ability is enhanced in aged cells
Mesenchymal stem cells (MSCs) are a potential source of adult stem cells for cell-based therapeutics due to their substantial multilineage differentiation capacity and secretory functions. No information is presently available regarding the maintenance of immunosuppressive properties of this cell ty...
Gespeichert in:
| Veröffentlicht in: | Molecular medicine reports 2015-01, Vol.11 (1), p.166-174 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 174 |
|---|---|
| container_issue | 1 |
| container_start_page | 166 |
| container_title | Molecular medicine reports |
| container_volume | 11 |
| creator | ZHUANG, YONG LI, DONG FU, JINQIU SHI, QING LU, YUANYUAN JU, XIULI |
| description | Mesenchymal stem cells (MSCs) are a potential source of adult stem cells for cell-based therapeutics due to their substantial multilineage differentiation capacity and secretory functions. No information is presently available regarding the maintenance of immunosuppressive properties of this cell type with repeated passages. It was therefore the aim of the present study to analyze the biological properties, particularly the immunoregulatory effect, of MSCs from late passages. The differences between young and old MSCs in morphology, cell surface antigen phenotype, proliferation, gene expression and immunomodulatory ability were investigated. The results of the current study demonstrated that with the passage of cells, senescent MSCs displayed a characteristically enlarged and flattened morphology, different gene expression profiles and stronger immunosuppressive activities. Increased interleukin-6 production may be a possible underlying mechanism for this enhanced immunomodulatory ability of MSCs. These findings suggest that aged MSCs may provide a treatment option for patients with graft versus host disease and other diseases associated with dysregulation of the immune system. |
| doi_str_mv | 10.3892/mmr.2014.2755 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4237101</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A401380287</galeid><sourcerecordid>A401380287</sourcerecordid><originalsourceid>FETCH-LOGICAL-c510t-c69bc6d220952038c95c664107c145a05fd5936b6c53ef447ff80f7eccf80d883</originalsourceid><addsrcrecordid>eNptks9vFCEUxydGY2v16NWQeNDLrPwYmMFDk2ZTtUkTL3omLLzZpRlghJkm-wf5f5Zx19UaA8kj7334woNvVb0meMU6ST94n1YUk2ZFW86fVOeklaRmGDdPj2sqZXtWvcj5DmPBKZfPqzPKGZNU8PPq5zr6USeXY0CxRxsXh7h1Rg9oTHGENDnIS2H2Gzf8ypuYbG0huXuwyEOGYHZ7Xwp5Ao8MDENGfYoegU7DHulg0aAnQKPOWW8hf0Q33s8h-mjnUoipMIv2tEcuIwg7HUxRdgEV2h4EX1bPej1keHWMF9X3T9ff1l_q26-fb9ZXt7XhBE-1EXJjhKUUS04x64zkRoiG4NaQhmvMe8slExthOIO-adq-73DfgjEl2q5jF9XlQXecNx6sgTAlPagxOa_TXkXt1ONKcDu1jfeqoawlmBSB90eBFH_MkCflXV5a0AHinBUR5XJcNi0u6Nt_0Ls4p1DaU0Qy2giBO_aH2uoBlAt9LOeaRVRdNeXADtOuLdTqP1QZFrwzMUDvSv7RhvqwwaSYc4L-1CPBavGVKr5Si6_U4qvCv_n7YU70byMV4N0ByGP5cWdjPjFFqSakxssUgj0ATo_Ylg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1932466083</pqid></control><display><type>article</type><title>Comparison of biological properties of umbilical cord-derived mesenchymal stem cells from early and late passages: Immunomodulatory ability is enhanced in aged cells</title><source>Spandidos Publications Journals</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>ZHUANG, YONG ; LI, DONG ; FU, JINQIU ; SHI, QING ; LU, YUANYUAN ; JU, XIULI</creator><creatorcontrib>ZHUANG, YONG ; LI, DONG ; FU, JINQIU ; SHI, QING ; LU, YUANYUAN ; JU, XIULI</creatorcontrib><description>Mesenchymal stem cells (MSCs) are a potential source of adult stem cells for cell-based therapeutics due to their substantial multilineage differentiation capacity and secretory functions. No information is presently available regarding the maintenance of immunosuppressive properties of this cell type with repeated passages. It was therefore the aim of the present study to analyze the biological properties, particularly the immunoregulatory effect, of MSCs from late passages. The differences between young and old MSCs in morphology, cell surface antigen phenotype, proliferation, gene expression and immunomodulatory ability were investigated. The results of the current study demonstrated that with the passage of cells, senescent MSCs displayed a characteristically enlarged and flattened morphology, different gene expression profiles and stronger immunosuppressive activities. Increased interleukin-6 production may be a possible underlying mechanism for this enhanced immunomodulatory ability of MSCs. These findings suggest that aged MSCs may provide a treatment option for patients with graft versus host disease and other diseases associated with dysregulation of the immune system.</description><identifier>ISSN: 1791-2997</identifier><identifier>EISSN: 1791-3004</identifier><identifier>DOI: 10.3892/mmr.2014.2755</identifier><identifier>PMID: 25339265</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Antigens ; Antigens, Surface - metabolism ; beta-Galactosidase - metabolism ; Biosynthesis ; Cell Differentiation ; Cell Proliferation ; Cell surface ; Cellular Senescence - physiology ; Comparative analysis ; Computational Biology - methods ; Cytokines - genetics ; Cytokines - metabolism ; Cytology ; Dehydrogenases ; Flow cytometry ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Genes ; Genetic aspects ; Graft-versus-host reaction ; Humans ; Immune system ; Immunomodulation ; Immunomodulators ; immunomodulatory ability ; Immunophenotyping ; Immunoregulation ; Immunosuppression ; Interleukin 6 ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Mesenchymal Stromal Cells - ultrastructure ; Mesenchyme ; Metabolism ; Molecular Sequence Annotation ; Morphology ; Ontology ; Physical characteristics ; Physiological aspects ; Senescence ; Stem cells ; Umbilical cord ; Umbilical Cord - cytology ; umbilical cord-mesenchymal stem cell</subject><ispartof>Molecular medicine reports, 2015-01, Vol.11 (1), p.166-174</ispartof><rights>Copyright © 2015, Spandidos Publications</rights><rights>COPYRIGHT 2015 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2015</rights><rights>Copyright © 2015, Spandidos Publications 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-c69bc6d220952038c95c664107c145a05fd5936b6c53ef447ff80f7eccf80d883</citedby><cites>FETCH-LOGICAL-c510t-c69bc6d220952038c95c664107c145a05fd5936b6c53ef447ff80f7eccf80d883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,5556,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25339265$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZHUANG, YONG</creatorcontrib><creatorcontrib>LI, DONG</creatorcontrib><creatorcontrib>FU, JINQIU</creatorcontrib><creatorcontrib>SHI, QING</creatorcontrib><creatorcontrib>LU, YUANYUAN</creatorcontrib><creatorcontrib>JU, XIULI</creatorcontrib><title>Comparison of biological properties of umbilical cord-derived mesenchymal stem cells from early and late passages: Immunomodulatory ability is enhanced in aged cells</title><title>Molecular medicine reports</title><addtitle>Mol Med Rep</addtitle><description>Mesenchymal stem cells (MSCs) are a potential source of adult stem cells for cell-based therapeutics due to their substantial multilineage differentiation capacity and secretory functions. No information is presently available regarding the maintenance of immunosuppressive properties of this cell type with repeated passages. It was therefore the aim of the present study to analyze the biological properties, particularly the immunoregulatory effect, of MSCs from late passages. The differences between young and old MSCs in morphology, cell surface antigen phenotype, proliferation, gene expression and immunomodulatory ability were investigated. The results of the current study demonstrated that with the passage of cells, senescent MSCs displayed a characteristically enlarged and flattened morphology, different gene expression profiles and stronger immunosuppressive activities. Increased interleukin-6 production may be a possible underlying mechanism for this enhanced immunomodulatory ability of MSCs. These findings suggest that aged MSCs may provide a treatment option for patients with graft versus host disease and other diseases associated with dysregulation of the immune system.</description><subject>Antigens</subject><subject>Antigens, Surface - metabolism</subject><subject>beta-Galactosidase - metabolism</subject><subject>Biosynthesis</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cell surface</subject><subject>Cellular Senescence - physiology</subject><subject>Comparative analysis</subject><subject>Computational Biology - methods</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>Cytology</subject><subject>Dehydrogenases</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Graft-versus-host reaction</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunomodulation</subject><subject>Immunomodulators</subject><subject>immunomodulatory ability</subject><subject>Immunophenotyping</subject><subject>Immunoregulation</subject><subject>Immunosuppression</subject><subject>Interleukin 6</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mesenchymal Stromal Cells - ultrastructure</subject><subject>Mesenchyme</subject><subject>Metabolism</subject><subject>Molecular Sequence Annotation</subject><subject>Morphology</subject><subject>Ontology</subject><subject>Physical characteristics</subject><subject>Physiological aspects</subject><subject>Senescence</subject><subject>Stem cells</subject><subject>Umbilical cord</subject><subject>Umbilical Cord - cytology</subject><subject>umbilical cord-mesenchymal stem cell</subject><issn>1791-2997</issn><issn>1791-3004</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptks9vFCEUxydGY2v16NWQeNDLrPwYmMFDk2ZTtUkTL3omLLzZpRlghJkm-wf5f5Zx19UaA8kj7334woNvVb0meMU6ST94n1YUk2ZFW86fVOeklaRmGDdPj2sqZXtWvcj5DmPBKZfPqzPKGZNU8PPq5zr6USeXY0CxRxsXh7h1Rg9oTHGENDnIS2H2Gzf8ypuYbG0huXuwyEOGYHZ7Xwp5Ao8MDENGfYoegU7DHulg0aAnQKPOWW8hf0Q33s8h-mjnUoipMIv2tEcuIwg7HUxRdgEV2h4EX1bPej1keHWMF9X3T9ff1l_q26-fb9ZXt7XhBE-1EXJjhKUUS04x64zkRoiG4NaQhmvMe8slExthOIO-adq-73DfgjEl2q5jF9XlQXecNx6sgTAlPagxOa_TXkXt1ONKcDu1jfeqoawlmBSB90eBFH_MkCflXV5a0AHinBUR5XJcNi0u6Nt_0Ls4p1DaU0Qy2giBO_aH2uoBlAt9LOeaRVRdNeXADtOuLdTqP1QZFrwzMUDvSv7RhvqwwaSYc4L-1CPBavGVKr5Si6_U4qvCv_n7YU70byMV4N0ByGP5cWdjPjFFqSakxssUgj0ATo_Ylg</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>ZHUANG, YONG</creator><creator>LI, DONG</creator><creator>FU, JINQIU</creator><creator>SHI, QING</creator><creator>LU, YUANYUAN</creator><creator>JU, XIULI</creator><general>D.A. Spandidos</general><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Comparison of biological properties of umbilical cord-derived mesenchymal stem cells from early and late passages: Immunomodulatory ability is enhanced in aged cells</title><author>ZHUANG, YONG ; LI, DONG ; FU, JINQIU ; SHI, QING ; LU, YUANYUAN ; JU, XIULI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-c69bc6d220952038c95c664107c145a05fd5936b6c53ef447ff80f7eccf80d883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antigens</topic><topic>Antigens, Surface - metabolism</topic><topic>beta-Galactosidase - metabolism</topic><topic>Biosynthesis</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cell surface</topic><topic>Cellular Senescence - physiology</topic><topic>Comparative analysis</topic><topic>Computational Biology - methods</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>Cytology</topic><topic>Dehydrogenases</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Graft-versus-host reaction</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunomodulation</topic><topic>Immunomodulators</topic><topic>immunomodulatory ability</topic><topic>Immunophenotyping</topic><topic>Immunoregulation</topic><topic>Immunosuppression</topic><topic>Interleukin 6</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mesenchymal Stromal Cells - ultrastructure</topic><topic>Mesenchyme</topic><topic>Metabolism</topic><topic>Molecular Sequence Annotation</topic><topic>Morphology</topic><topic>Ontology</topic><topic>Physical characteristics</topic><topic>Physiological aspects</topic><topic>Senescence</topic><topic>Stem cells</topic><topic>Umbilical cord</topic><topic>Umbilical Cord - cytology</topic><topic>umbilical cord-mesenchymal stem cell</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZHUANG, YONG</creatorcontrib><creatorcontrib>LI, DONG</creatorcontrib><creatorcontrib>FU, JINQIU</creatorcontrib><creatorcontrib>SHI, QING</creatorcontrib><creatorcontrib>LU, YUANYUAN</creatorcontrib><creatorcontrib>JU, XIULI</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Molecular medicine reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHUANG, YONG</au><au>LI, DONG</au><au>FU, JINQIU</au><au>SHI, QING</au><au>LU, YUANYUAN</au><au>JU, XIULI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of biological properties of umbilical cord-derived mesenchymal stem cells from early and late passages: Immunomodulatory ability is enhanced in aged cells</atitle><jtitle>Molecular medicine reports</jtitle><addtitle>Mol Med Rep</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>11</volume><issue>1</issue><spage>166</spage><epage>174</epage><pages>166-174</pages><issn>1791-2997</issn><eissn>1791-3004</eissn><abstract>Mesenchymal stem cells (MSCs) are a potential source of adult stem cells for cell-based therapeutics due to their substantial multilineage differentiation capacity and secretory functions. No information is presently available regarding the maintenance of immunosuppressive properties of this cell type with repeated passages. It was therefore the aim of the present study to analyze the biological properties, particularly the immunoregulatory effect, of MSCs from late passages. The differences between young and old MSCs in morphology, cell surface antigen phenotype, proliferation, gene expression and immunomodulatory ability were investigated. The results of the current study demonstrated that with the passage of cells, senescent MSCs displayed a characteristically enlarged and flattened morphology, different gene expression profiles and stronger immunosuppressive activities. Increased interleukin-6 production may be a possible underlying mechanism for this enhanced immunomodulatory ability of MSCs. These findings suggest that aged MSCs may provide a treatment option for patients with graft versus host disease and other diseases associated with dysregulation of the immune system.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>25339265</pmid><doi>10.3892/mmr.2014.2755</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1791-2997 |
| ispartof | Molecular medicine reports, 2015-01, Vol.11 (1), p.166-174 |
| issn | 1791-2997 1791-3004 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4237101 |
| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Antigens Antigens, Surface - metabolism beta-Galactosidase - metabolism Biosynthesis Cell Differentiation Cell Proliferation Cell surface Cellular Senescence - physiology Comparative analysis Computational Biology - methods Cytokines - genetics Cytokines - metabolism Cytology Dehydrogenases Flow cytometry Gene expression Gene Expression Profiling Gene Expression Regulation Genes Genetic aspects Graft-versus-host reaction Humans Immune system Immunomodulation Immunomodulators immunomodulatory ability Immunophenotyping Immunoregulation Immunosuppression Interleukin 6 Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Mesenchymal Stromal Cells - ultrastructure Mesenchyme Metabolism Molecular Sequence Annotation Morphology Ontology Physical characteristics Physiological aspects Senescence Stem cells Umbilical cord Umbilical Cord - cytology umbilical cord-mesenchymal stem cell |
| title | Comparison of biological properties of umbilical cord-derived mesenchymal stem cells from early and late passages: Immunomodulatory ability is enhanced in aged cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T22%3A22%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20biological%20properties%20of%20umbilical%20cord-derived%20mesenchymal%20stem%20cells%20from%20early%20and%20late%20passages:%20Immunomodulatory%20ability%20is%20enhanced%20in%20aged%20cells&rft.jtitle=Molecular%20medicine%20reports&rft.au=ZHUANG,%20YONG&rft.date=2015-01-01&rft.volume=11&rft.issue=1&rft.spage=166&rft.epage=174&rft.pages=166-174&rft.issn=1791-2997&rft.eissn=1791-3004&rft_id=info:doi/10.3892/mmr.2014.2755&rft_dat=%3Cgale_pubme%3EA401380287%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1932466083&rft_id=info:pmid/25339265&rft_galeid=A401380287&rfr_iscdi=true |