The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans
Streptococcus mutans is known to be a primary etiological factor of dental caries, a widespread and growing disease in Polish children. Recognition of novel features determining the pathogenicity of this pathogen may contribute to understanding the mechanisms of bacterial infections.The goal of the...
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| Veröffentlicht in: | BMC microbiology 2014-08, Vol.14 (1), p.194-194, Article 194 |
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| creator | Krzyściak, Wirginia Pluskwa, Katarzyna K Piątkowski, Jakub Krzyściak, Paweł Jurczak, Anna Kościelniak, Dorota Skalniak, Anna |
| description | Streptococcus mutans is known to be a primary etiological factor of dental caries, a widespread and growing disease in Polish children. Recognition of novel features determining the pathogenicity of this pathogen may contribute to understanding the mechanisms of bacterial infections.The goal of the study was to determine the activity of prephenate dehydrogenase (PHD) and to illuminate the role of the enzyme in S. mutans pathogenicity. The strains were biotyped based on STREPTOtest 24 biochemical identification tests and the usefulness of biotyping in the determination of S. mutans pathogenicity determinants was examined.
Out of ninety strains isolated from children with deciduous teeth fifty three were classified as S. mutans species. PDH activity was higher (21.69 U/mg on average) in the experimental group compared to the control group (5.74 U/mg on average) (P |
| doi_str_mv | 10.1186/1471-2180-14-194 |
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Out of ninety strains isolated from children with deciduous teeth fifty three were classified as S. mutans species. PDH activity was higher (21.69 U/mg on average) in the experimental group compared to the control group (5.74 U/mg on average) (P <0.001). Moreover, it was demonstrated that biotype I, established basing on the biochemical characterization of the strain, was predominant (58.5%) in oral cavity streptococcosis. Its dominance was determined by higher PDH activity compared to biotypes II and III (P = 0.0019).
The usefulness of biotyping in the determination of Streptococcus mutans pathogenicity determinants was demonstrated. The obtained results allow for better differentiation of S. mutans species and thus may contribute to recognition of pathogenic bacteria transmission mechanisms and facilitate treatment.</description><identifier>ISSN: 1471-2180</identifier><identifier>EISSN: 1471-2180</identifier><identifier>DOI: 10.1186/1471-2180-14-194</identifier><identifier>PMID: 25096795</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Bacteria ; Bacterial Typing Techniques - methods ; Bacteriology ; Biofilms ; Biosynthesis ; Care and treatment ; Child ; Child, Preschool ; Data analysis ; Dehydrogenases ; Dental caries ; Dentistry ; Enzymes ; Female ; Humans ; Laboratories ; Male ; Medical research ; Methods ; Microbiology ; Mouth - microbiology ; Physiological aspects ; Prephenate Dehydrogenase - analysis ; Risk factors ; Streptococcus mutans ; Streptococcus mutans - classification ; Streptococcus mutans - enzymology ; Streptococcus mutans - isolation & purification ; Streptococcus mutans - pathogenicity ; Studies ; Teeth ; Virulence Factors - analysis</subject><ispartof>BMC microbiology, 2014-08, Vol.14 (1), p.194-194, Article 194</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>2014 Krzysciak et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>Copyright © 2014 Krzyściak et al.; licensee BioMed Central Ltd. 2014 Krzyściak et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-1d056ba1ca83ef36948da62a91999ee04c7a411d2fc8729f6df575ea461367793</citedby><cites>FETCH-LOGICAL-c558t-1d056ba1ca83ef36948da62a91999ee04c7a411d2fc8729f6df575ea461367793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236502/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236502/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25096795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krzyściak, Wirginia</creatorcontrib><creatorcontrib>Pluskwa, Katarzyna K</creatorcontrib><creatorcontrib>Piątkowski, Jakub</creatorcontrib><creatorcontrib>Krzyściak, Paweł</creatorcontrib><creatorcontrib>Jurczak, Anna</creatorcontrib><creatorcontrib>Kościelniak, Dorota</creatorcontrib><creatorcontrib>Skalniak, Anna</creatorcontrib><title>The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans</title><title>BMC microbiology</title><addtitle>BMC Microbiol</addtitle><description>Streptococcus mutans is known to be a primary etiological factor of dental caries, a widespread and growing disease in Polish children. Recognition of novel features determining the pathogenicity of this pathogen may contribute to understanding the mechanisms of bacterial infections.The goal of the study was to determine the activity of prephenate dehydrogenase (PHD) and to illuminate the role of the enzyme in S. mutans pathogenicity. The strains were biotyped based on STREPTOtest 24 biochemical identification tests and the usefulness of biotyping in the determination of S. mutans pathogenicity determinants was examined.
Out of ninety strains isolated from children with deciduous teeth fifty three were classified as S. mutans species. PDH activity was higher (21.69 U/mg on average) in the experimental group compared to the control group (5.74 U/mg on average) (P <0.001). Moreover, it was demonstrated that biotype I, established basing on the biochemical characterization of the strain, was predominant (58.5%) in oral cavity streptococcosis. Its dominance was determined by higher PDH activity compared to biotypes II and III (P = 0.0019).
The usefulness of biotyping in the determination of Streptococcus mutans pathogenicity determinants was demonstrated. The obtained results allow for better differentiation of S. mutans species and thus may contribute to recognition of pathogenic bacteria transmission mechanisms and facilitate treatment.</description><subject>Bacteria</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biosynthesis</subject><subject>Care and treatment</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Data analysis</subject><subject>Dehydrogenases</subject><subject>Dental caries</subject><subject>Dentistry</subject><subject>Enzymes</subject><subject>Female</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medical research</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Mouth - microbiology</subject><subject>Physiological aspects</subject><subject>Prephenate Dehydrogenase - analysis</subject><subject>Risk factors</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - classification</subject><subject>Streptococcus mutans - enzymology</subject><subject>Streptococcus mutans - isolation & purification</subject><subject>Streptococcus mutans - pathogenicity</subject><subject>Studies</subject><subject>Teeth</subject><subject>Virulence Factors - analysis</subject><issn>1471-2180</issn><issn>1471-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNks1rFTEUxQdRbK3uXcmAG11MTTL5mGyEUvwoFARb1yEvczMvZSYZk4z4_nsztr72iQsJIZeb3zlcLqeqXmJ0inHH32EqcENwhxpMGyzpo-p433r8oD6qnqV0gxAWXSueVkeEIcmFZMdVuN5CvSSwy-ghpTrYeuNC3s3OD7XzdS7fPWSIk_M6u-BXIsEIJkNfzzpvwwDeGZd395zPadVe5QhzDiYYs6R6WrL26Xn1xOoxwYu796T69vHD9fnn5vLLp4vzs8vGMNblBveI8Y3GRnct2JZL2vWaEy2xlBIAUSM0xbgn1nSCSMt7ywQDTTluuRCyPane3_rOy2aC3oDPUY9qjm7ScaeCdurwx7utGsIPRUnLGSLF4M2dQQzfF0hZTS4ZGEftISxJYcYRpkh06D9QxjihLV3Hev0XehOW6MsmflPlcknuqUGPoJy3oYxoVlN1xlrJBCZ89Tr9B1VOD5MzwYN1pX8geHsgKEyGn3nQS0rq4urrIYtuWRNDShHsfnUYqTV7ag2XWsNVKlWyVySvHq58L_gTtvYX_zjTxw</recordid><startdate>20140805</startdate><enddate>20140805</enddate><creator>Krzyściak, Wirginia</creator><creator>Pluskwa, Katarzyna K</creator><creator>Piątkowski, Jakub</creator><creator>Krzyściak, Paweł</creator><creator>Jurczak, Anna</creator><creator>Kościelniak, Dorota</creator><creator>Skalniak, Anna</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140805</creationdate><title>The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans</title><author>Krzyściak, Wirginia ; Pluskwa, Katarzyna K ; Piątkowski, Jakub ; Krzyściak, Paweł ; Jurczak, Anna ; Kościelniak, Dorota ; Skalniak, Anna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-1d056ba1ca83ef36948da62a91999ee04c7a411d2fc8729f6df575ea461367793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacteria</topic><topic>Bacterial Typing Techniques - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krzyściak, Wirginia</au><au>Pluskwa, Katarzyna K</au><au>Piątkowski, Jakub</au><au>Krzyściak, Paweł</au><au>Jurczak, Anna</au><au>Kościelniak, Dorota</au><au>Skalniak, Anna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans</atitle><jtitle>BMC microbiology</jtitle><addtitle>BMC Microbiol</addtitle><date>2014-08-05</date><risdate>2014</risdate><volume>14</volume><issue>1</issue><spage>194</spage><epage>194</epage><pages>194-194</pages><artnum>194</artnum><issn>1471-2180</issn><eissn>1471-2180</eissn><abstract>Streptococcus mutans is known to be a primary etiological factor of dental caries, a widespread and growing disease in Polish children. Recognition of novel features determining the pathogenicity of this pathogen may contribute to understanding the mechanisms of bacterial infections.The goal of the study was to determine the activity of prephenate dehydrogenase (PHD) and to illuminate the role of the enzyme in S. mutans pathogenicity. The strains were biotyped based on STREPTOtest 24 biochemical identification tests and the usefulness of biotyping in the determination of S. mutans pathogenicity determinants was examined.
Out of ninety strains isolated from children with deciduous teeth fifty three were classified as S. mutans species. PDH activity was higher (21.69 U/mg on average) in the experimental group compared to the control group (5.74 U/mg on average) (P <0.001). Moreover, it was demonstrated that biotype I, established basing on the biochemical characterization of the strain, was predominant (58.5%) in oral cavity streptococcosis. Its dominance was determined by higher PDH activity compared to biotypes II and III (P = 0.0019).
The usefulness of biotyping in the determination of Streptococcus mutans pathogenicity determinants was demonstrated. The obtained results allow for better differentiation of S. mutans species and thus may contribute to recognition of pathogenic bacteria transmission mechanisms and facilitate treatment.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25096795</pmid><doi>10.1186/1471-2180-14-194</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteria Bacterial Typing Techniques - methods Bacteriology Biofilms Biosynthesis Care and treatment Child Child, Preschool Data analysis Dehydrogenases Dental caries Dentistry Enzymes Female Humans Laboratories Male Medical research Methods Microbiology Mouth - microbiology Physiological aspects Prephenate Dehydrogenase - analysis Risk factors Streptococcus mutans Streptococcus mutans - classification Streptococcus mutans - enzymology Streptococcus mutans - isolation & purification Streptococcus mutans - pathogenicity Studies Teeth Virulence Factors - analysis |
| title | The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans |
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