1,25-dihydroxyvitamin D3 production by human keratinocytes: kinetics and regulation
Human foreskin keratinocytes in vitro metabolize 25-hydroxyvitamin D3 to a number of metabolites, including 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). This metabolite remains mostly within the cell and does not accumulate in the medium under the conditions of these experiments. With time, 1,25(OH)2D3 i...
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| Veröffentlicht in: | The Journal of clinical investigation 1986-08, Vol.78 (2), p.557-566 |
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| description | Human foreskin keratinocytes in vitro metabolize 25-hydroxyvitamin D3 to a number of metabolites, including 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). This metabolite remains mostly within the cell and does not accumulate in the medium under the conditions of these experiments. With time, 1,25(OH)2D3 is catabolized, and more polar metabolites appear in both the cells and the medium. The production of 1,25(OH)2D3 has an apparent Michaelis constant (Km) for 25-hydroxyvitamin D3 of 5.4 X 10(-8) M. The levels of 1,25(OH)2D3 within the cell are increased both by increased production and decreased catabolism when parathyroid hormone(1-34) and isobutylmethylxanthine are added. Exogenously added 1,25(OH)2D3 at concentrations as low as 10(-12) M reduces endogenous 1,25(OH)2D3 production, increases 1,25(OH)2D3 catabolism, and increases 24,25-dihydroxyvitamin D3 production by an actinomycin D-sensitive process. These data indicate that the regulation of 1,25(OH)2D3 production by keratinocytes is similar to, but not identical to the regulation of 1,25(OH)2D3 by the kidney. |
| doi_str_mv | 10.1172/jci112609 |
| format | Article |
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Exogenously added 1,25(OH)2D3 at concentrations as low as 10(-12) M reduces endogenous 1,25(OH)2D3 production, increases 1,25(OH)2D3 catabolism, and increases 24,25-dihydroxyvitamin D3 production by an actinomycin D-sensitive process. These data indicate that the regulation of 1,25(OH)2D3 production by keratinocytes is similar to, but not identical to the regulation of 1,25(OH)2D3 by the kidney.</description><identifier>ISSN: 0021-9738</identifier><identifier>EISSN: 1558-8238</identifier><identifier>DOI: 10.1172/jci112609</identifier><identifier>PMID: 2426308</identifier><identifier>CODEN: JCINAO</identifier><language>eng</language><publisher>Ann Arbor, MI: American Society for Clinical Investigation</publisher><subject>1-Methyl-3-isobutylxanthine - pharmacology ; Biological and medical sciences ; Calcitriol - biosynthesis ; Calcitriol - metabolism ; Calcium - pharmacology ; Cells, Cultured ; Culture Media ; Fundamental and applied biological sciences. Psychology ; Humans ; Keratins ; Kinetics ; Parathyroid Hormone - pharmacology ; Skin - cytology ; Skin - drug effects ; Skin - metabolism ; Substrate Specificity ; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><ispartof>The Journal of clinical investigation, 1986-08, Vol.78 (2), p.557-566</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-36a78d5082dab9c6041b56ef48e9cb1fff8eaa574841795d11b19c9496f7ec5e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC423594/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC423594/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8191710$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2426308$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BIKLE, D. D</creatorcontrib><creatorcontrib>NEMANIC, M. K</creatorcontrib><creatorcontrib>GEE, E</creatorcontrib><creatorcontrib>ELIAS, P</creatorcontrib><title>1,25-dihydroxyvitamin D3 production by human keratinocytes: kinetics and regulation</title><title>The Journal of clinical investigation</title><addtitle>J Clin Invest</addtitle><description>Human foreskin keratinocytes in vitro metabolize 25-hydroxyvitamin D3 to a number of metabolites, including 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). This metabolite remains mostly within the cell and does not accumulate in the medium under the conditions of these experiments. With time, 1,25(OH)2D3 is catabolized, and more polar metabolites appear in both the cells and the medium. The production of 1,25(OH)2D3 has an apparent Michaelis constant (Km) for 25-hydroxyvitamin D3 of 5.4 X 10(-8) M. The levels of 1,25(OH)2D3 within the cell are increased both by increased production and decreased catabolism when parathyroid hormone(1-34) and isobutylmethylxanthine are added. Exogenously added 1,25(OH)2D3 at concentrations as low as 10(-12) M reduces endogenous 1,25(OH)2D3 production, increases 1,25(OH)2D3 catabolism, and increases 24,25-dihydroxyvitamin D3 production by an actinomycin D-sensitive process. These data indicate that the regulation of 1,25(OH)2D3 production by keratinocytes is similar to, but not identical to the regulation of 1,25(OH)2D3 by the kidney.</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Calcitriol - biosynthesis</subject><subject>Calcitriol - metabolism</subject><subject>Calcium - pharmacology</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Keratins</subject><subject>Kinetics</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>Skin - cytology</subject><subject>Skin - drug effects</subject><subject>Skin - metabolism</subject><subject>Substrate Specificity</subject><subject>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><issn>0021-9738</issn><issn>1558-8238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU9v1DAQxS1EVZbCgQ-AlANCQiLF4_gvEge0QFtUiQNwtiaO03Wb2Fs7qci3Z6tdreA0h_d7M6P3CHkF9BxAsQ-3LgAwSc0TsgIhdK1Zo5-SFaUMaqMa_Yw8L-WWUuBc8FNyyjiTDdUr8hPeM1F3YbN0Of1ZHsKEY4jVl6ba5tTNbgopVu1SbeYRY3XnM04hJrdMvnys7kL0U3ClwthV2d_MAz7yL8hJj0PxLw_zjPz-9vXX-rK-_nFxtf58XTsu-VQ3EpXuBNWsw9Y4STm0Qvqea29cC33fa48oFNcclBEdQAvGGW5kr7wTvjkjn_Z7t3M7-s75OGUc7DaHEfNiEwb7vxLDxt6kB8tZIwzf-d8e_Dndz75MdgzF-WHA6NNcrJJGKKHkDny3B11OpWTfH28AtY8F2O_rq30BO_b1v08dyUPiO_3NQcficOgzRhfKEdNgQAFt_gInko8N</recordid><startdate>19860801</startdate><enddate>19860801</enddate><creator>BIKLE, D. D</creator><creator>NEMANIC, M. K</creator><creator>GEE, E</creator><creator>ELIAS, P</creator><general>American Society for Clinical Investigation</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19860801</creationdate><title>1,25-dihydroxyvitamin D3 production by human keratinocytes: kinetics and regulation</title><author>BIKLE, D. D ; NEMANIC, M. K ; GEE, E ; ELIAS, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-36a78d5082dab9c6041b56ef48e9cb1fff8eaa574841795d11b19c9496f7ec5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Calcitriol - biosynthesis</topic><topic>Calcitriol - metabolism</topic><topic>Calcium - pharmacology</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Keratins</topic><topic>Kinetics</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>Skin - cytology</topic><topic>Skin - drug effects</topic><topic>Skin - metabolism</topic><topic>Substrate Specificity</topic><topic>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BIKLE, D. D</creatorcontrib><creatorcontrib>NEMANIC, M. K</creatorcontrib><creatorcontrib>GEE, E</creatorcontrib><creatorcontrib>ELIAS, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of clinical investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BIKLE, D. D</au><au>NEMANIC, M. K</au><au>GEE, E</au><au>ELIAS, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>1,25-dihydroxyvitamin D3 production by human keratinocytes: kinetics and regulation</atitle><jtitle>The Journal of clinical investigation</jtitle><addtitle>J Clin Invest</addtitle><date>1986-08-01</date><risdate>1986</risdate><volume>78</volume><issue>2</issue><spage>557</spage><epage>566</epage><pages>557-566</pages><issn>0021-9738</issn><eissn>1558-8238</eissn><coden>JCINAO</coden><abstract>Human foreskin keratinocytes in vitro metabolize 25-hydroxyvitamin D3 to a number of metabolites, including 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). This metabolite remains mostly within the cell and does not accumulate in the medium under the conditions of these experiments. With time, 1,25(OH)2D3 is catabolized, and more polar metabolites appear in both the cells and the medium. The production of 1,25(OH)2D3 has an apparent Michaelis constant (Km) for 25-hydroxyvitamin D3 of 5.4 X 10(-8) M. The levels of 1,25(OH)2D3 within the cell are increased both by increased production and decreased catabolism when parathyroid hormone(1-34) and isobutylmethylxanthine are added. Exogenously added 1,25(OH)2D3 at concentrations as low as 10(-12) M reduces endogenous 1,25(OH)2D3 production, increases 1,25(OH)2D3 catabolism, and increases 24,25-dihydroxyvitamin D3 production by an actinomycin D-sensitive process. These data indicate that the regulation of 1,25(OH)2D3 production by keratinocytes is similar to, but not identical to the regulation of 1,25(OH)2D3 by the kidney.</abstract><cop>Ann Arbor, MI</cop><pub>American Society for Clinical Investigation</pub><pmid>2426308</pmid><doi>10.1172/jci112609</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 1-Methyl-3-isobutylxanthine - pharmacology Biological and medical sciences Calcitriol - biosynthesis Calcitriol - metabolism Calcium - pharmacology Cells, Cultured Culture Media Fundamental and applied biological sciences. Psychology Humans Keratins Kinetics Parathyroid Hormone - pharmacology Skin - cytology Skin - drug effects Skin - metabolism Substrate Specificity Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue |
| title | 1,25-dihydroxyvitamin D3 production by human keratinocytes: kinetics and regulation |
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