Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis
Francisella isolates from patients suffering from tularemia in Germany are generally strains of the species F. tularensis subsp. holarctica. To our knowledge, no other Francisella species are known for Germany. Recently, a new Francisella species could be isolated from a water reservoir of a cooling...
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| Veröffentlicht in: | BMC microbiology 2014-06, Vol.14 (1), p.169-169, Article 169 |
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| creator | Rydzewski, Kerstin Schulz, Tino Brzuszkiewicz, Elzbieta Holland, Gudrun Lück, Christian Fleischer, Jens Grunow, Roland Heuner, Klaus |
| description | Francisella isolates from patients suffering from tularemia in Germany are generally strains of the species F. tularensis subsp. holarctica. To our knowledge, no other Francisella species are known for Germany. Recently, a new Francisella species could be isolated from a water reservoir of a cooling tower in Germany.
We identified a Francisella sp. (isolate W12-1067) whose 16S rDNA is 99% identical to the respective nucleotide sequence of the recently published strain F. guangzhouensis. The overall sequence identity of the fopA, gyrA, rpoA, groEL, sdhA and dnaK genes is only 89%, indicating that strain W12-1067 is not identical to F. guangzhouensis. W12-1067 was isolated from a water reservoir of a cooling tower of a hospital in Germany. The growth optimum of the isolate is approximately 30°C, it can grow in the presence of 4-5% NaCl (halotolerant) and is able to grow without additional cysteine within the medium. The strain was able to replicate within a mouse-derived macrophage-like cell line. The whole genome of the strain was sequenced (~1.7 mbp, 32.2% G + C content) and the draft genome was annotated. Various virulence genes common to the genus Francisella are present, but the Francisella pathogenicity island (FPI) is missing. However, another putative type-VI secretion system is present within the genome of strain W12-1067.
Isolate W12-1067 is closely related to the recently described F. guangzhouensis species and it replicates within eukaryotic host cells. Since W12-1067 exhibits a putative new type-VI secretion system and F. tularensis subsp. holarctica was found not to be the sole species in Germany, the new isolate is an interesting species to be analyzed in more detail. Further research is needed to investigate the epidemiology, ecology and pathogenicity of Francisella species present in Germany. |
| doi_str_mv | 10.1186/1471-2180-14-169 |
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We identified a Francisella sp. (isolate W12-1067) whose 16S rDNA is 99% identical to the respective nucleotide sequence of the recently published strain F. guangzhouensis. The overall sequence identity of the fopA, gyrA, rpoA, groEL, sdhA and dnaK genes is only 89%, indicating that strain W12-1067 is not identical to F. guangzhouensis. W12-1067 was isolated from a water reservoir of a cooling tower of a hospital in Germany. The growth optimum of the isolate is approximately 30°C, it can grow in the presence of 4-5% NaCl (halotolerant) and is able to grow without additional cysteine within the medium. The strain was able to replicate within a mouse-derived macrophage-like cell line. The whole genome of the strain was sequenced (~1.7 mbp, 32.2% G + C content) and the draft genome was annotated. Various virulence genes common to the genus Francisella are present, but the Francisella pathogenicity island (FPI) is missing. However, another putative type-VI secretion system is present within the genome of strain W12-1067.
Isolate W12-1067 is closely related to the recently described F. guangzhouensis species and it replicates within eukaryotic host cells. Since W12-1067 exhibits a putative new type-VI secretion system and F. tularensis subsp. holarctica was found not to be the sole species in Germany, the new isolate is an interesting species to be analyzed in more detail. Further research is needed to investigate the epidemiology, ecology and pathogenicity of Francisella species present in Germany.</description><identifier>ISSN: 1471-2180</identifier><identifier>EISSN: 1471-2180</identifier><identifier>DOI: 10.1186/1471-2180-14-169</identifier><identifier>PMID: 24961323</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Animals ; Bacteria ; Bacteriology ; Cell Line ; Chitinase ; Cluster Analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Experiments ; Francisella ; Francisella - genetics ; Francisella - growth & development ; Francisella - isolation & purification ; Francisella - physiology ; Francisella tularensis ; Genome, Bacterial ; Genomes ; Germany ; Humans ; Laboratories ; Macrophages - microbiology ; Mice ; Molecular Sequence Data ; Plasmids ; Proteins ; Sequence Analysis, DNA ; Sequence Homology ; Sodium Chloride - metabolism ; Temperature ; Tularemia ; Virulence Factors - genetics ; Water Microbiology ; Yeast</subject><ispartof>BMC microbiology, 2014-06, Vol.14 (1), p.169-169, Article 169</ispartof><rights>2014 Rydzewski et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>Copyright © 2014 Rydzewski et al.; licensee BioMed Central Ltd. 2014 Rydzewski et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-ad6edc6d7363b86a2610f31c64263db6da77e4f176788347aaf31ce7f9e2cf8d3</citedby><cites>FETCH-LOGICAL-c457t-ad6edc6d7363b86a2610f31c64263db6da77e4f176788347aaf31ce7f9e2cf8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230796/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4230796/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24961323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rydzewski, Kerstin</creatorcontrib><creatorcontrib>Schulz, Tino</creatorcontrib><creatorcontrib>Brzuszkiewicz, Elzbieta</creatorcontrib><creatorcontrib>Holland, Gudrun</creatorcontrib><creatorcontrib>Lück, Christian</creatorcontrib><creatorcontrib>Fleischer, Jens</creatorcontrib><creatorcontrib>Grunow, Roland</creatorcontrib><creatorcontrib>Heuner, Klaus</creatorcontrib><title>Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis</title><title>BMC microbiology</title><addtitle>BMC Microbiol</addtitle><description>Francisella isolates from patients suffering from tularemia in Germany are generally strains of the species F. tularensis subsp. holarctica. To our knowledge, no other Francisella species are known for Germany. Recently, a new Francisella species could be isolated from a water reservoir of a cooling tower in Germany.
We identified a Francisella sp. (isolate W12-1067) whose 16S rDNA is 99% identical to the respective nucleotide sequence of the recently published strain F. guangzhouensis. The overall sequence identity of the fopA, gyrA, rpoA, groEL, sdhA and dnaK genes is only 89%, indicating that strain W12-1067 is not identical to F. guangzhouensis. W12-1067 was isolated from a water reservoir of a cooling tower of a hospital in Germany. The growth optimum of the isolate is approximately 30°C, it can grow in the presence of 4-5% NaCl (halotolerant) and is able to grow without additional cysteine within the medium. The strain was able to replicate within a mouse-derived macrophage-like cell line. The whole genome of the strain was sequenced (~1.7 mbp, 32.2% G + C content) and the draft genome was annotated. Various virulence genes common to the genus Francisella are present, but the Francisella pathogenicity island (FPI) is missing. However, another putative type-VI secretion system is present within the genome of strain W12-1067.
Isolate W12-1067 is closely related to the recently described F. guangzhouensis species and it replicates within eukaryotic host cells. Since W12-1067 exhibits a putative new type-VI secretion system and F. tularensis subsp. holarctica was found not to be the sole species in Germany, the new isolate is an interesting species to be analyzed in more detail. Further research is needed to investigate the epidemiology, ecology and pathogenicity of Francisella species present in Germany.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Cell Line</subject><subject>Chitinase</subject><subject>Cluster Analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Experiments</subject><subject>Francisella</subject><subject>Francisella - genetics</subject><subject>Francisella - growth & development</subject><subject>Francisella - isolation & purification</subject><subject>Francisella - physiology</subject><subject>Francisella tularensis</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Germany</subject><subject>Humans</subject><subject>Laboratories</subject><subject>Macrophages - microbiology</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Sodium Chloride - metabolism</subject><subject>Temperature</subject><subject>Tularemia</subject><subject>Virulence Factors - genetics</subject><subject>Water Microbiology</subject><subject>Yeast</subject><issn>1471-2180</issn><issn>1471-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNUsFu1DAQjRCIlsKdE7LEpT2k9diJ7VyQUEUXpEpcijhaXmey6yqxg51U2r_gk3G6ZdX21JPHM2_eePxeUXwEeg6gxAVUEkoGipZQlSCaV8XxIfX6UXxUvEvpllKQisu3xRGrGgGc8ePi7wp9GJAk_DOjt0iMb8m4zclpNzqbr6bfJZdI6IghnYtpIiuMg_HkKhpvXcK-NySN58Sl0JsJyelvYCVQIc9IZiFr7IPfOL8hUyDTNo8a0TpMT_qnuTcRfR70vnjTmT7hh4fzpPh19e3m8nt5_XP14_LrdWmrWk6laQW2VrSSC75WwjABtONgRcUEb9eiNVJi1YEUUileSWOWKsquQWY71fKT4sued5zXQ6ZCP0XT6zG6wcSdDsbppxXvtnoT7nTFOJWNyASnDwQx5L9Lkx5csss2HsOcNNRCKE6bhr0AWquaqfoe-vkZ9DbMMYuwR4FqauAZRfcoG0NKEbvDu4HqxRl6kV4v0udIZ2fklk-P9z00_LcC_wfQdbSu</recordid><startdate>20140625</startdate><enddate>20140625</enddate><creator>Rydzewski, Kerstin</creator><creator>Schulz, Tino</creator><creator>Brzuszkiewicz, Elzbieta</creator><creator>Holland, Gudrun</creator><creator>Lück, Christian</creator><creator>Fleischer, Jens</creator><creator>Grunow, Roland</creator><creator>Heuner, Klaus</creator><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TM</scope><scope>5PM</scope></search><sort><creationdate>20140625</creationdate><title>Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis</title><author>Rydzewski, Kerstin ; Schulz, Tino ; Brzuszkiewicz, Elzbieta ; Holland, Gudrun ; Lück, Christian ; Fleischer, Jens ; Grunow, Roland ; Heuner, Klaus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-ad6edc6d7363b86a2610f31c64263db6da77e4f176788347aaf31ce7f9e2cf8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Cell Line</topic><topic>Chitinase</topic><topic>Cluster Analysis</topic><topic>DNA, Bacterial - 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Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rydzewski, Kerstin</au><au>Schulz, Tino</au><au>Brzuszkiewicz, Elzbieta</au><au>Holland, Gudrun</au><au>Lück, Christian</au><au>Fleischer, Jens</au><au>Grunow, Roland</au><au>Heuner, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis</atitle><jtitle>BMC microbiology</jtitle><addtitle>BMC Microbiol</addtitle><date>2014-06-25</date><risdate>2014</risdate><volume>14</volume><issue>1</issue><spage>169</spage><epage>169</epage><pages>169-169</pages><artnum>169</artnum><issn>1471-2180</issn><eissn>1471-2180</eissn><abstract>Francisella isolates from patients suffering from tularemia in Germany are generally strains of the species F. tularensis subsp. holarctica. To our knowledge, no other Francisella species are known for Germany. Recently, a new Francisella species could be isolated from a water reservoir of a cooling tower in Germany.
We identified a Francisella sp. (isolate W12-1067) whose 16S rDNA is 99% identical to the respective nucleotide sequence of the recently published strain F. guangzhouensis. The overall sequence identity of the fopA, gyrA, rpoA, groEL, sdhA and dnaK genes is only 89%, indicating that strain W12-1067 is not identical to F. guangzhouensis. W12-1067 was isolated from a water reservoir of a cooling tower of a hospital in Germany. The growth optimum of the isolate is approximately 30°C, it can grow in the presence of 4-5% NaCl (halotolerant) and is able to grow without additional cysteine within the medium. The strain was able to replicate within a mouse-derived macrophage-like cell line. The whole genome of the strain was sequenced (~1.7 mbp, 32.2% G + C content) and the draft genome was annotated. Various virulence genes common to the genus Francisella are present, but the Francisella pathogenicity island (FPI) is missing. However, another putative type-VI secretion system is present within the genome of strain W12-1067.
Isolate W12-1067 is closely related to the recently described F. guangzhouensis species and it replicates within eukaryotic host cells. Since W12-1067 exhibits a putative new type-VI secretion system and F. tularensis subsp. holarctica was found not to be the sole species in Germany, the new isolate is an interesting species to be analyzed in more detail. Further research is needed to investigate the epidemiology, ecology and pathogenicity of Francisella species present in Germany.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>24961323</pmid><doi>10.1186/1471-2180-14-169</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bacteria Bacteriology Cell Line Chitinase Cluster Analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics Experiments Francisella Francisella - genetics Francisella - growth & development Francisella - isolation & purification Francisella - physiology Francisella tularensis Genome, Bacterial Genomes Germany Humans Laboratories Macrophages - microbiology Mice Molecular Sequence Data Plasmids Proteins Sequence Analysis, DNA Sequence Homology Sodium Chloride - metabolism Temperature Tularemia Virulence Factors - genetics Water Microbiology Yeast |
| title | Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis |
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