Isolation of a Differentially Regulated Splicing Isoform of Human NF-E2
The transcription factor NF-E2 (nuclear factor erythroid 2), interacting via DNA motifs within regulatory regions of several hematopoietic genes, is thought to mediate the enhancer activity of the globin locus control regions. By screening a human fetal liver cDNA library with probes derived from mo...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1995-04, Vol.92 (8), p.3511-3515 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Pischedda, Carla Cocco, Sandro Melis, Antonello Marini, Maria Giuseppina Kan, Yuet Wai Cao, Antonio Moi, Paolo |
| description | The transcription factor NF-E2 (nuclear factor erythroid 2), interacting via DNA motifs within regulatory regions of several hematopoietic genes, is thought to mediate the enhancer activity of the globin locus control regions. By screening a human fetal liver cDNA library with probes derived from mouse NF-E2, we have isolated a splicing variant of the NF-E2 gene (fNF-E2) that differs in the 5' untranslated region from the previously reported cDNA (aNF-E2). The fNF-E2 isoform is transcribed from an alternative promoter located in the 3' end of the first intron and joined by alternative splicing to the second and third exons, which are shared by both RNA isoforms. Although the two forms produce the same protein, they are expressed in different ratios during development. fNF-E2 is more abundant in the fetal liver and less abundant in the adult bone marrow compared to the previously described form. Their distribution apparently follows the differential expression of fetal and adult hemoglobins. |
| doi_str_mv | 10.1073/pnas.92.8.3511 |
| format | Article |
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By screening a human fetal liver cDNA library with probes derived from mouse NF-E2, we have isolated a splicing variant of the NF-E2 gene (fNF-E2) that differs in the 5' untranslated region from the previously reported cDNA (aNF-E2). The fNF-E2 isoform is transcribed from an alternative promoter located in the 3' end of the first intron and joined by alternative splicing to the second and third exons, which are shared by both RNA isoforms. Although the two forms produce the same protein, they are expressed in different ratios during development. fNF-E2 is more abundant in the fetal liver and less abundant in the adult bone marrow compared to the previously described form. Their distribution apparently follows the differential expression of fetal and adult hemoglobins.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.92.8.3511</identifier><identifier>PMID: 7724591</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Alternative Splicing ; Amino Acid Sequence ; Base Sequence ; Biochemistry ; cDNA ; Cell-Free System ; Cloning, Molecular ; Complementary DNA ; Deoxyribonucleic acid ; DNA ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Erythroid-Specific DNA-Binding Factors ; Exons ; Fetus ; Five prime untranslated regions ; Gene Expression Regulation ; Gene Library ; Genes ; hemoglobin ; Hemoglobins - biosynthesis ; Humans ; Liver ; man ; Messenger RNA ; Molecular Sequence Data ; NF-E2 protein ; NF-E2 Transcription Factor ; NF-E2 Transcription Factor, p45 Subunit ; nuclear factor erythroid 2 ; nucleotide sequence ; Promoter regions ; Promoter Regions, Genetic - genetics ; Protein Binding ; Protein Biosynthesis ; Protein isoforms ; Rodents ; splicing ; Tissue Distribution ; transcription factors ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic ; Untranslated regions</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1995-04, Vol.92 (8), p.3511-3515</ispartof><rights>Copyright 1995 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Apr 11, 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c581t-8d66c2cdaa99079583a5fa310ab639e04739c8207b0287e8cc156f87fc4cfd513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/92/8.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2367097$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2367097$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7724591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pischedda, Carla</creatorcontrib><creatorcontrib>Cocco, Sandro</creatorcontrib><creatorcontrib>Melis, Antonello</creatorcontrib><creatorcontrib>Marini, Maria Giuseppina</creatorcontrib><creatorcontrib>Kan, Yuet Wai</creatorcontrib><creatorcontrib>Cao, Antonio</creatorcontrib><creatorcontrib>Moi, Paolo</creatorcontrib><title>Isolation of a Differentially Regulated Splicing Isoform of Human NF-E2</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The transcription factor NF-E2 (nuclear factor erythroid 2), interacting via DNA motifs within regulatory regions of several hematopoietic genes, is thought to mediate the enhancer activity of the globin locus control regions. By screening a human fetal liver cDNA library with probes derived from mouse NF-E2, we have isolated a splicing variant of the NF-E2 gene (fNF-E2) that differs in the 5' untranslated region from the previously reported cDNA (aNF-E2). The fNF-E2 isoform is transcribed from an alternative promoter located in the 3' end of the first intron and joined by alternative splicing to the second and third exons, which are shared by both RNA isoforms. Although the two forms produce the same protein, they are expressed in different ratios during development. fNF-E2 is more abundant in the fetal liver and less abundant in the adult bone marrow compared to the previously described form. Their distribution apparently follows the differential expression of fetal and adult hemoglobins.</description><subject>Alternative Splicing</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>cDNA</subject><subject>Cell-Free System</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Erythroid-Specific DNA-Binding Factors</subject><subject>Exons</subject><subject>Fetus</subject><subject>Five prime untranslated regions</subject><subject>Gene Expression Regulation</subject><subject>Gene Library</subject><subject>Genes</subject><subject>hemoglobin</subject><subject>Hemoglobins - biosynthesis</subject><subject>Humans</subject><subject>Liver</subject><subject>man</subject><subject>Messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>NF-E2 protein</subject><subject>NF-E2 Transcription Factor</subject><subject>NF-E2 Transcription Factor, p45 Subunit</subject><subject>nuclear factor erythroid 2</subject><subject>nucleotide sequence</subject><subject>Promoter regions</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>Protein Biosynthesis</subject><subject>Protein isoforms</subject><subject>Rodents</subject><subject>splicing</subject><subject>Tissue Distribution</subject><subject>transcription factors</subject><subject>Transcription Factors - biosynthesis</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Untranslated regions</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctv1DAYxC0EKtvClRNIEYfeEj7b8UviUpW-pAokHmfL69hLVt54sRNE__s62mW1cICTD_ObT-MZhF5haDAI-m47mNwo0siGMoyfoAUGhWveKniKFgBE1LIl7XN0mvMaABSTcIJOhCAtU3iBbu5yDGbs41BFX5nqQ--9S24YexPCQ_XZraYiu676sg297YdVVQw-ps2M304bM1Qfr-sr8gI98yZk93L_nqFv11dfL2_r-083d5cX97VlEo-17Di3xHbGKAWihKGGeUMxmCWnykErqLKSgFgCkcJJazHjXgpvW-s7hukZer-7u52WG9fZkjSZoLep35j0oKPp9Z_K0H_Xq_hTtwQrUezne3uKPyaXR73ps3UhmMHFKevSC-EE0_-CmAuGGWEFfPsXuI5TGkoHmgCmwDjnBWp2kE0x5-T8ITAGPc-o5xm1IlrqecZieHP8zQO-3-1In32_1WP_-b907acQRvdrLODrHbjOY0wHklAuoPT1CJqfuNo</recordid><startdate>19950411</startdate><enddate>19950411</enddate><creator>Pischedda, Carla</creator><creator>Cocco, Sandro</creator><creator>Melis, Antonello</creator><creator>Marini, Maria Giuseppina</creator><creator>Kan, Yuet Wai</creator><creator>Cao, Antonio</creator><creator>Moi, Paolo</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7T3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19950411</creationdate><title>Isolation of a Differentially Regulated Splicing Isoform of Human NF-E2</title><author>Pischedda, Carla ; Cocco, Sandro ; Melis, Antonello ; Marini, Maria Giuseppina ; Kan, Yuet Wai ; Cao, Antonio ; Moi, Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c581t-8d66c2cdaa99079583a5fa310ab639e04739c8207b0287e8cc156f87fc4cfd513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alternative Splicing</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>cDNA</topic><topic>Cell-Free System</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Erythroid-Specific DNA-Binding Factors</topic><topic>Exons</topic><topic>Fetus</topic><topic>Five prime untranslated regions</topic><topic>Gene Expression Regulation</topic><topic>Gene Library</topic><topic>Genes</topic><topic>hemoglobin</topic><topic>Hemoglobins - biosynthesis</topic><topic>Humans</topic><topic>Liver</topic><topic>man</topic><topic>Messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>NF-E2 protein</topic><topic>NF-E2 Transcription Factor</topic><topic>NF-E2 Transcription Factor, p45 Subunit</topic><topic>nuclear factor erythroid 2</topic><topic>nucleotide sequence</topic><topic>Promoter regions</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>Protein Biosynthesis</topic><topic>Protein isoforms</topic><topic>Rodents</topic><topic>splicing</topic><topic>Tissue Distribution</topic><topic>transcription factors</topic><topic>Transcription Factors - biosynthesis</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Untranslated regions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pischedda, Carla</creatorcontrib><creatorcontrib>Cocco, Sandro</creatorcontrib><creatorcontrib>Melis, Antonello</creatorcontrib><creatorcontrib>Marini, Maria Giuseppina</creatorcontrib><creatorcontrib>Kan, Yuet Wai</creatorcontrib><creatorcontrib>Cao, Antonio</creatorcontrib><creatorcontrib>Moi, Paolo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Human Genome Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pischedda, Carla</au><au>Cocco, Sandro</au><au>Melis, Antonello</au><au>Marini, Maria Giuseppina</au><au>Kan, Yuet Wai</au><au>Cao, Antonio</au><au>Moi, Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of a Differentially Regulated Splicing Isoform of Human NF-E2</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1995-04-11</date><risdate>1995</risdate><volume>92</volume><issue>8</issue><spage>3511</spage><epage>3515</epage><pages>3511-3515</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>The transcription factor NF-E2 (nuclear factor erythroid 2), interacting via DNA motifs within regulatory regions of several hematopoietic genes, is thought to mediate the enhancer activity of the globin locus control regions. By screening a human fetal liver cDNA library with probes derived from mouse NF-E2, we have isolated a splicing variant of the NF-E2 gene (fNF-E2) that differs in the 5' untranslated region from the previously reported cDNA (aNF-E2). The fNF-E2 isoform is transcribed from an alternative promoter located in the 3' end of the first intron and joined by alternative splicing to the second and third exons, which are shared by both RNA isoforms. Although the two forms produce the same protein, they are expressed in different ratios during development. fNF-E2 is more abundant in the fetal liver and less abundant in the adult bone marrow compared to the previously described form. Their distribution apparently follows the differential expression of fetal and adult hemoglobins.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>7724591</pmid><doi>10.1073/pnas.92.8.3511</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1995-04, Vol.92 (8), p.3511-3515 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Alternative Splicing Amino Acid Sequence Base Sequence Biochemistry cDNA Cell-Free System Cloning, Molecular Complementary DNA Deoxyribonucleic acid DNA DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Erythroid-Specific DNA-Binding Factors Exons Fetus Five prime untranslated regions Gene Expression Regulation Gene Library Genes hemoglobin Hemoglobins - biosynthesis Humans Liver man Messenger RNA Molecular Sequence Data NF-E2 protein NF-E2 Transcription Factor NF-E2 Transcription Factor, p45 Subunit nuclear factor erythroid 2 nucleotide sequence Promoter regions Promoter Regions, Genetic - genetics Protein Binding Protein Biosynthesis Protein isoforms Rodents splicing Tissue Distribution transcription factors Transcription Factors - biosynthesis Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic Untranslated regions |
| title | Isolation of a Differentially Regulated Splicing Isoform of Human NF-E2 |
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