A compact Airy beam light sheet microscope with a tilted cylindrical lens
Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagati...
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| Veröffentlicht in: | Biomedical optics express 2014-10, Vol.5 (10), p.3434-3442 |
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| container_title | Biomedical optics express |
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| creator | Yang, Zhengyi Prokopas, Martynas Nylk, Jonathan Coll-Lladó, Clara Gunn-Moore, Frank J Ferrier, David E K Vettenburg, Tom Dholakia, Kishan |
| description | Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality. |
| doi_str_mv | 10.1364/BOE.5.003434 |
| format | Article |
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Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. 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| ispartof | Biomedical optics express, 2014-10, Vol.5 (10), p.3434-3442 |
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| language | eng |
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| title | A compact Airy beam light sheet microscope with a tilted cylindrical lens |
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