Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration
To realize cardiac regeneration using human induced pluripotent stem cells (hiPSCs), strategies for cell preparation, tissue engineering and transplantation must be explored. Here we report a new protocol for the simultaneous induction of cardiomyocytes (CMs) and vascular cells [endothelial cells (E...
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| Veröffentlicht in: | Scientific reports 2014-10, Vol.4 (1), p.6716-6716, Article 6716 |
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| creator | Masumoto, Hidetoshi Ikuno, Takeshi Takeda, Masafumi Fukushima, Hiroyuki Marui, Akira Katayama, Shiori Shimizu, Tatsuya Ikeda, Tadashi Okano, Teruo Sakata, Ryuzo Yamashita, Jun K. |
| description | To realize cardiac regeneration using human induced pluripotent stem cells (hiPSCs), strategies for cell preparation, tissue engineering and transplantation must be explored. Here we report a new protocol for the simultaneous induction of cardiomyocytes (CMs) and vascular cells [endothelial cells (ECs)/vascular mural cells (MCs)] and generate entirely hiPSC-engineered cardiovascular cell sheets, which showed advantageous therapeutic effects in infarcted hearts. The protocol adds to a previous differentiation protocol of CMs by using stage-specific supplementation of vascular endothelial cell growth factor for the additional induction of vascular cells. Using this cell sheet technology, we successfully generated physically integrated cardiac tissue sheets (hiPSC-CTSs). HiPSC-CTS transplantation to rat infarcted hearts significantly improved cardiac function. In addition to neovascularization, we confirmed that engrafted human cells mainly consisted of CMs in >40% of transplanted rats four weeks after transplantation. Thus, our HiPSC-CTSs show promise for cardiac regenerative therapy. |
| doi_str_mv | 10.1038/srep06716 |
| format | Article |
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Here we report a new protocol for the simultaneous induction of cardiomyocytes (CMs) and vascular cells [endothelial cells (ECs)/vascular mural cells (MCs)] and generate entirely hiPSC-engineered cardiovascular cell sheets, which showed advantageous therapeutic effects in infarcted hearts. The protocol adds to a previous differentiation protocol of CMs by using stage-specific supplementation of vascular endothelial cell growth factor for the additional induction of vascular cells. Using this cell sheet technology, we successfully generated physically integrated cardiac tissue sheets (hiPSC-CTSs). HiPSC-CTS transplantation to rat infarcted hearts significantly improved cardiac function. In addition to neovascularization, we confirmed that engrafted human cells mainly consisted of CMs in >40% of transplanted rats four weeks after transplantation. Thus, our HiPSC-CTSs show promise for cardiac regenerative therapy.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep06716</identifier><identifier>PMID: 25336194</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13/100 ; 13/107 ; 631/532/2064/2158 ; 631/532/489 ; 692/308/2171 ; 692/699/75/230 ; Animals ; Cardiomyocytes ; Cell Differentiation - genetics ; Endothelial cells ; Endothelial Cells - cytology ; Endothelial Cells - transplantation ; Heart ; Heart diseases ; Heart transplantation ; Humanities and Social Sciences ; Humans ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - transplantation ; multidisciplinary ; Myocardial Infarction - pathology ; Myocardial Infarction - therapy ; Myocytes, Cardiac - cytology ; Myocytes, Cardiac - transplantation ; Pluripotency ; Rats ; Regeneration ; Rodents ; Science ; Stem cell transplantation ; Stem cells ; Supplements ; Tissue Engineering ; Transplantation ; Vascularization</subject><ispartof>Scientific reports, 2014-10, Vol.4 (1), p.6716-6716, Article 6716</ispartof><rights>The Author(s) 2014</rights><rights>Copyright Nature Publishing Group Oct 2014</rights><rights>Copyright © 2014, Macmillan Publishers Limited. All rights reserved 2014 Macmillan Publishers Limited. 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Here we report a new protocol for the simultaneous induction of cardiomyocytes (CMs) and vascular cells [endothelial cells (ECs)/vascular mural cells (MCs)] and generate entirely hiPSC-engineered cardiovascular cell sheets, which showed advantageous therapeutic effects in infarcted hearts. The protocol adds to a previous differentiation protocol of CMs by using stage-specific supplementation of vascular endothelial cell growth factor for the additional induction of vascular cells. Using this cell sheet technology, we successfully generated physically integrated cardiac tissue sheets (hiPSC-CTSs). HiPSC-CTS transplantation to rat infarcted hearts significantly improved cardiac function. In addition to neovascularization, we confirmed that engrafted human cells mainly consisted of CMs in >40% of transplanted rats four weeks after transplantation. Thus, our HiPSC-CTSs show promise for cardiac regenerative therapy.</description><subject>13/100</subject><subject>13/107</subject><subject>631/532/2064/2158</subject><subject>631/532/489</subject><subject>692/308/2171</subject><subject>692/699/75/230</subject><subject>Animals</subject><subject>Cardiomyocytes</subject><subject>Cell Differentiation - genetics</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - transplantation</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Heart transplantation</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Induced Pluripotent Stem Cells - cytology</subject><subject>Induced Pluripotent Stem Cells - transplantation</subject><subject>multidisciplinary</subject><subject>Myocardial Infarction - pathology</subject><subject>Myocardial Infarction - therapy</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masumoto, Hidetoshi</au><au>Ikuno, Takeshi</au><au>Takeda, Masafumi</au><au>Fukushima, Hiroyuki</au><au>Marui, Akira</au><au>Katayama, Shiori</au><au>Shimizu, Tatsuya</au><au>Ikeda, Tadashi</au><au>Okano, Teruo</au><au>Sakata, Ryuzo</au><au>Yamashita, Jun K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2014-10-22</date><risdate>2014</risdate><volume>4</volume><issue>1</issue><spage>6716</spage><epage>6716</epage><pages>6716-6716</pages><artnum>6716</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>To realize cardiac regeneration using human induced pluripotent stem cells (hiPSCs), strategies for cell preparation, tissue engineering and transplantation must be explored. Here we report a new protocol for the simultaneous induction of cardiomyocytes (CMs) and vascular cells [endothelial cells (ECs)/vascular mural cells (MCs)] and generate entirely hiPSC-engineered cardiovascular cell sheets, which showed advantageous therapeutic effects in infarcted hearts. The protocol adds to a previous differentiation protocol of CMs by using stage-specific supplementation of vascular endothelial cell growth factor for the additional induction of vascular cells. Using this cell sheet technology, we successfully generated physically integrated cardiac tissue sheets (hiPSC-CTSs). HiPSC-CTS transplantation to rat infarcted hearts significantly improved cardiac function. In addition to neovascularization, we confirmed that engrafted human cells mainly consisted of CMs in >40% of transplanted rats four weeks after transplantation. Thus, our HiPSC-CTSs show promise for cardiac regenerative therapy.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>25336194</pmid><doi>10.1038/srep06716</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 13/100 13/107 631/532/2064/2158 631/532/489 692/308/2171 692/699/75/230 Animals Cardiomyocytes Cell Differentiation - genetics Endothelial cells Endothelial Cells - cytology Endothelial Cells - transplantation Heart Heart diseases Heart transplantation Humanities and Social Sciences Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - transplantation multidisciplinary Myocardial Infarction - pathology Myocardial Infarction - therapy Myocytes, Cardiac - cytology Myocytes, Cardiac - transplantation Pluripotency Rats Regeneration Rodents Science Stem cell transplantation Stem cells Supplements Tissue Engineering Transplantation Vascularization |
| title | Human iPS cell-engineered cardiac tissue sheets with cardiomyocytes and vascular cells for cardiac regeneration |
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