Mutations of mitochondrial 12S rRNA in gastric carcinoma and their significance
AIM: To detect the variations of mitochondrial 12S rRNA in patients with gastric carcinoma, and to study their significance and the relationship between these variations and the genesis of gastric carcinoma. METHODS: PCR amplified mitochondrial 12S rRNA of 44 samples including 22 from gastric carcin...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2005-01, Vol.11 (1), p.31-35 |
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| creator | Han, Cheng-Bo Ma, Jia-Ming Xin, Yan Mao, Xiao-Yun Zhao, Yu-Jie Wu, Dong-Ying Zhang, Su-Min Zhang, Yu-Kui |
| description | AIM: To detect the variations of mitochondrial 12S rRNA in patients with gastric carcinoma, and to study their significance and the relationship between these variations and the genesis of gastric carcinoma. METHODS: PCR amplified mitochondrial 12S rRNA of 44 samples including 22 from gastric carcinoma tissues and 22from adjacent normal tissues, was detected by direct DNA sequencing. Then laser capture microdissection technique (LCM) was used to separate the cancerous cells and dysplasia cells with specific mutations. Denaturing high performance liquid chromatography (DHPLC) plus allele-specific PCR (AS-PCR), nest-PCR and polyacrylamide gel electrophoresis (PAGE) were used to further evaluate this mutant property and quantitative difference of mutant type between cancerous and dysplasia cells. Finally, RNAdraw biosoft was used to analyze the RNA secondary structure of mutant-type 12S rRNA. RESULTS: Compared with Mitomap database, some new variations were found, among which np652 G insertion and np716 T-G transversion were found only in cancerous tissues. There was a statistic difference in the frequency of 12S rRNA variation between intestinal type (12/17, 70.59%) and diffusive type (5/17, 29.41%) of gastric carcinoma (P |
| doi_str_mv | 10.3748/wjg.v11.i1.31 |
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METHODS: PCR amplified mitochondrial 12S rRNA of 44 samples including 22 from gastric carcinoma tissues and 22from adjacent normal tissues, was detected by direct DNA sequencing. Then laser capture microdissection technique (LCM) was used to separate the cancerous cells and dysplasia cells with specific mutations. Denaturing high performance liquid chromatography (DHPLC) plus allele-specific PCR (AS-PCR), nest-PCR and polyacrylamide gel electrophoresis (PAGE) were used to further evaluate this mutant property and quantitative difference of mutant type between cancerous and dysplasia cells. Finally, RNAdraw biosoft was used to analyze the RNA secondary structure of mutant-type 12S rRNA. RESULTS: Compared with Mitomap database, some new variations were found, among which np652 G insertion and np716 T-G transversion were found only in cancerous tissues. There was a statistic difference in the frequency of 12S rRNA variation between intestinal type (12/17, 70.59%) and diffusive type (5/17, 29.41%) of gastric carcinoma (P<0.05). DHPLC analysis showed that 12S rRNA np652 G insertion and np716 T-G transversion were heteroplasmic mutations. The frequency of 12S rRNA variation in cancerous cells was higher than that in dysplasia cells (P<0.01). 12S rRNA np652 G insertion showed obviously negative effects on the stability of 12S rRNA secondary structure, while others such as T-G transversion did not. CONCLUSION: The mutations of mitochondrial 12S rRNA may be associated with the occurrence of intestinal-type gastric carcinoma. Most variations exist both in gastric carcinomas and in normal tissues, and they might not be the characteristics of tumors. However, np652 G insertion and np716 T-G transversion may possess some molecular significance in gastric carcinogenesis. During the process from normality to dysplasia, then to carcinoma, 12S rRNA tends to convert from homoplasmy (wild type) to heteroplasmy, then to homoplasmy (mutant type, np717 T-G).</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v11.i1.31</identifier><identifier>PMID: 15609392</identifier><language>eng</language><publisher>United States: Cancer Institute, the First Affiliated Hospital, China Medical University, S henyang 110001, Liaoning Province, China%Biochips Center, China Medical University, Shenyang 110001, Liaoning Province, China</publisher><subject>12S ; Base Sequence ; Gastric Cancer ; Genetic Variation ; Humans ; Molecular Sequence Data ; Nucleic Acid Conformation ; Point Mutation ; RNA - chemistry ; RNA - genetics ; RNA, Mitochondrial ; RNA, Ribosomal - chemistry ; RNA, Ribosomal - genetics ; rRNA ; Stomach Neoplasms - classification ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; 基因突变 ; 线粒体</subject><ispartof>World journal of gastroenterology : WJG, 2005-01, Vol.11 (1), p.31-35</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2005 Baishideng Publishing Group Inc. All rights reserved. 2005</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-870210ad93a31d59ee3c9c823ae22805336a8561d3510aa966f26e97fe57b9df3</citedby><cites>FETCH-LOGICAL-c441t-870210ad93a31d59ee3c9c823ae22805336a8561d3510aa966f26e97fe57b9df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205379/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4205379/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15609392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Cheng-Bo</creatorcontrib><creatorcontrib>Ma, Jia-Ming</creatorcontrib><creatorcontrib>Xin, Yan</creatorcontrib><creatorcontrib>Mao, Xiao-Yun</creatorcontrib><creatorcontrib>Zhao, Yu-Jie</creatorcontrib><creatorcontrib>Wu, Dong-Ying</creatorcontrib><creatorcontrib>Zhang, Su-Min</creatorcontrib><creatorcontrib>Zhang, Yu-Kui</creatorcontrib><title>Mutations of mitochondrial 12S rRNA in gastric carcinoma and their significance</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM: To detect the variations of mitochondrial 12S rRNA in patients with gastric carcinoma, and to study their significance and the relationship between these variations and the genesis of gastric carcinoma. METHODS: PCR amplified mitochondrial 12S rRNA of 44 samples including 22 from gastric carcinoma tissues and 22from adjacent normal tissues, was detected by direct DNA sequencing. Then laser capture microdissection technique (LCM) was used to separate the cancerous cells and dysplasia cells with specific mutations. Denaturing high performance liquid chromatography (DHPLC) plus allele-specific PCR (AS-PCR), nest-PCR and polyacrylamide gel electrophoresis (PAGE) were used to further evaluate this mutant property and quantitative difference of mutant type between cancerous and dysplasia cells. Finally, RNAdraw biosoft was used to analyze the RNA secondary structure of mutant-type 12S rRNA. RESULTS: Compared with Mitomap database, some new variations were found, among which np652 G insertion and np716 T-G transversion were found only in cancerous tissues. There was a statistic difference in the frequency of 12S rRNA variation between intestinal type (12/17, 70.59%) and diffusive type (5/17, 29.41%) of gastric carcinoma (P<0.05). DHPLC analysis showed that 12S rRNA np652 G insertion and np716 T-G transversion were heteroplasmic mutations. The frequency of 12S rRNA variation in cancerous cells was higher than that in dysplasia cells (P<0.01). 12S rRNA np652 G insertion showed obviously negative effects on the stability of 12S rRNA secondary structure, while others such as T-G transversion did not. CONCLUSION: The mutations of mitochondrial 12S rRNA may be associated with the occurrence of intestinal-type gastric carcinoma. Most variations exist both in gastric carcinomas and in normal tissues, and they might not be the characteristics of tumors. However, np652 G insertion and np716 T-G transversion may possess some molecular significance in gastric carcinogenesis. During the process from normality to dysplasia, then to carcinoma, 12S rRNA tends to convert from homoplasmy (wild type) to heteroplasmy, then to homoplasmy (mutant type, np717 T-G).</description><subject>12S</subject><subject>Base Sequence</subject><subject>Gastric Cancer</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Point Mutation</subject><subject>RNA - chemistry</subject><subject>RNA - genetics</subject><subject>RNA, Mitochondrial</subject><subject>RNA, Ribosomal - chemistry</subject><subject>RNA, Ribosomal - genetics</subject><subject>rRNA</subject><subject>Stomach Neoplasms - classification</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><subject>基因突变</subject><subject>线粒体</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1vEzEQhi0EoqFw5IosxHWDx1571xekqqKAVFqpwNmaeO3NhMQu3k0r_n1dJeLjNId59MzofRl7DWKpurZ_f78Zl3cAS4KlgidsISXYRvateMoWIETXWCW7E_ZimjZCSKW0fM5OQBthlZULdv11P-NMOU08R76jOft1TkMh3HKQ33i5uTrjlPiI01zIc4_FU8o75JgGPq8DFT7RmCiSx-TDS_Ys4nYKr47zlP24-Pj9_HNzef3py_nZZePbFuam74QEgYNVqGDQNgTlre-lwiBlL7RSBnttYFC6YmiNidIE28Wgu5UdojplHw7e2_1qFwYf0lxw624L7bD8dhnJ_b9JtHZjvnOtrPbOVsG7g-AeU8Q0uk3el1RfdjVRKYQWNT1TseaA-ZKnqYT45wQI91jAI-5qAY7AKaj8m3__-ksfE6_A26Ow5jz-onp5hf5npG1wAKbT0Br1AJ7Bja8</recordid><startdate>20050107</startdate><enddate>20050107</enddate><creator>Han, Cheng-Bo</creator><creator>Ma, Jia-Ming</creator><creator>Xin, Yan</creator><creator>Mao, Xiao-Yun</creator><creator>Zhao, Yu-Jie</creator><creator>Wu, Dong-Ying</creator><creator>Zhang, Su-Min</creator><creator>Zhang, Yu-Kui</creator><general>Cancer Institute, the First Affiliated Hospital, China Medical University, S henyang 110001, Liaoning Province, China%Biochips Center, China Medical University, Shenyang 110001, Liaoning Province, China</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20050107</creationdate><title>Mutations of mitochondrial 12S rRNA in gastric carcinoma and their significance</title><author>Han, Cheng-Bo ; 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METHODS: PCR amplified mitochondrial 12S rRNA of 44 samples including 22 from gastric carcinoma tissues and 22from adjacent normal tissues, was detected by direct DNA sequencing. Then laser capture microdissection technique (LCM) was used to separate the cancerous cells and dysplasia cells with specific mutations. Denaturing high performance liquid chromatography (DHPLC) plus allele-specific PCR (AS-PCR), nest-PCR and polyacrylamide gel electrophoresis (PAGE) were used to further evaluate this mutant property and quantitative difference of mutant type between cancerous and dysplasia cells. Finally, RNAdraw biosoft was used to analyze the RNA secondary structure of mutant-type 12S rRNA. RESULTS: Compared with Mitomap database, some new variations were found, among which np652 G insertion and np716 T-G transversion were found only in cancerous tissues. There was a statistic difference in the frequency of 12S rRNA variation between intestinal type (12/17, 70.59%) and diffusive type (5/17, 29.41%) of gastric carcinoma (P<0.05). DHPLC analysis showed that 12S rRNA np652 G insertion and np716 T-G transversion were heteroplasmic mutations. The frequency of 12S rRNA variation in cancerous cells was higher than that in dysplasia cells (P<0.01). 12S rRNA np652 G insertion showed obviously negative effects on the stability of 12S rRNA secondary structure, while others such as T-G transversion did not. CONCLUSION: The mutations of mitochondrial 12S rRNA may be associated with the occurrence of intestinal-type gastric carcinoma. Most variations exist both in gastric carcinomas and in normal tissues, and they might not be the characteristics of tumors. However, np652 G insertion and np716 T-G transversion may possess some molecular significance in gastric carcinogenesis. During the process from normality to dysplasia, then to carcinoma, 12S rRNA tends to convert from homoplasmy (wild type) to heteroplasmy, then to homoplasmy (mutant type, np717 T-G).</abstract><cop>United States</cop><pub>Cancer Institute, the First Affiliated Hospital, China Medical University, S henyang 110001, Liaoning Province, China%Biochips Center, China Medical University, Shenyang 110001, Liaoning Province, China</pub><pmid>15609392</pmid><doi>10.3748/wjg.v11.i1.31</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 12S Base Sequence Gastric Cancer Genetic Variation Humans Molecular Sequence Data Nucleic Acid Conformation Point Mutation RNA - chemistry RNA - genetics RNA, Mitochondrial RNA, Ribosomal - chemistry RNA, Ribosomal - genetics rRNA Stomach Neoplasms - classification Stomach Neoplasms - genetics Stomach Neoplasms - pathology 基因突变 线粒体 |
| title | Mutations of mitochondrial 12S rRNA in gastric carcinoma and their significance |
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