High-Resolution Crystal Structures Reveal Plasticity in the Metal Binding Site of Apurinic/Apyrimidinic Endonuclease I

Apurinic/apyrimidinic endonuclease I (APE1) is an essential base excision repair enzyme that catalyzes a Mg2+-dependent reaction in which the phosphodiester backbone is cleaved 5′ of an abasic site in duplex DNA. This reaction has been proposed to involve either one or two metal ions bound to the ac...

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Veröffentlicht in:	Biochemistry (Easton) 2014-10, Vol.53 (41), p.6520-6529
Hauptverfasser:	He, Hongzhen, Chen, Qiujia, Georgiadis, Millie M
Format:	Artikel
Sprache:	eng
Schlagworte:	active sites Amino Acid Substitution Apoenzymes - chemistry Apoenzymes - genetics Apoenzymes - metabolism Binding Sites Biocatalysis Catalytic Domain crystal structure Crystallography, X-Ray DNA DNA - chemistry DNA - metabolism DNA repair DNA-(Apurinic or Apyrimidinic Site) Lyase - chemistry DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics DNA-(Apurinic or Apyrimidinic Site) Lyase - metabolism fluorometry Glutamic Acid - chemistry Humans Kinetics magnesium Magnesium - chemistry Magnesium - metabolism manganese Manganese - chemistry Manganese - metabolism metal ions Models, Molecular Mutagenesis, Site-Directed Mutant Proteins - chemistry Mutant Proteins - metabolism Peptide Fragments - chemistry Peptide Fragments - genetics Peptide Fragments - metabolism Pliability Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - metabolism
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