The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression
Collagen prolyl hydroxylases (C-P4HAs) are a family of enzymes involved in collagen biogenesis. One of the isoforms of P4HA, Prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), catalyzes the formation of 4-hydroxyproline that is essential for the proper three-dimensional folding of newly synthesized...
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| Veröffentlicht in: | Oncotarget 2014-08, Vol.5 (16), p.6654-6669 |
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| creator | Chakravarthi, Balabhadrapatruni V S K Pathi, Satya Sreehari Goswami, Moloy T Cieślik, Marcin Zheng, Heng Nallasivam, Sivakumar Arekapudi, Subramanyeswara R Jing, Xiaojun Siddiqui, Javed Athanikar, Jyoti Carskadon, Shannon L Lonigro, Robert J Kunju, Lakshmi P Chinnaiyan, Arul M Palanisamy, Nallasivam Varambally, Sooryanarayana |
| description | Collagen prolyl hydroxylases (C-P4HAs) are a family of enzymes involved in collagen biogenesis. One of the isoforms of P4HA, Prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), catalyzes the formation of 4-hydroxyproline that is essential for the proper three-dimensional folding of newly synthesized procollagen chains. Here, we show the overexpression of P4HA1 in aggressive prostate cancer. Immunohistochemical analysis using tissue microarray demonstrated that P4HA1 expression was correlated with prostate cancer progression. Using in vitro studies, we showed that P4HA1 plays a critical role in prostate cancer cell growth and tumor progression. Expression profiling studies using P4HA1 modulated prostate cells suggested regulation of Matrix metalloproteases 1. The invasive properties of P4HA1 overexpressing cells were reversed by blocking MMP1. Our studies indicate P4HA1 copy number gain in a subset of metastatic prostate tumors and its expression is also regulated by microRNA-124. MiR-124 in turn is negatively regulated by transcriptional repressors EZH2 and CtBP1, both of which are overexpressed in aggressive prostate cancer. Chick chorioallantoic membrane (CAM) assay and mice xenograft investigations show that P4HA1 is required for tumor growth and metastasis in vivo. Our observations suggest that P4HA1 plays a critical role in prostate cancer progression and could serve as a viable therapeutic target. |
| doi_str_mv | 10.18632/oncotarget.2208 |
| format | Article |
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One of the isoforms of P4HA, Prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), catalyzes the formation of 4-hydroxyproline that is essential for the proper three-dimensional folding of newly synthesized procollagen chains. Here, we show the overexpression of P4HA1 in aggressive prostate cancer. Immunohistochemical analysis using tissue microarray demonstrated that P4HA1 expression was correlated with prostate cancer progression. Using in vitro studies, we showed that P4HA1 plays a critical role in prostate cancer cell growth and tumor progression. Expression profiling studies using P4HA1 modulated prostate cells suggested regulation of Matrix metalloproteases 1. The invasive properties of P4HA1 overexpressing cells were reversed by blocking MMP1. Our studies indicate P4HA1 copy number gain in a subset of metastatic prostate tumors and its expression is also regulated by microRNA-124. MiR-124 in turn is negatively regulated by transcriptional repressors EZH2 and CtBP1, both of which are overexpressed in aggressive prostate cancer. Chick chorioallantoic membrane (CAM) assay and mice xenograft investigations show that P4HA1 is required for tumor growth and metastasis in vivo. Our observations suggest that P4HA1 plays a critical role in prostate cancer progression and could serve as a viable therapeutic target.</description><identifier>EISSN: 1949-2553</identifier><identifier>DOI: 10.18632/oncotarget.2208</identifier><identifier>PMID: 25115393</identifier><language>eng</language><publisher>United States: Impact Journals LLC</publisher><subject>Animals ; Cell Line, Tumor ; Cell Proliferation - physiology ; Disease Progression ; Gene Expression ; HEK293 Cells ; Heterografts ; Humans ; Male ; Matrix Metalloproteinase 1 - biosynthesis ; Matrix Metalloproteinase 1 - genetics ; Matrix Metalloproteinase 1 - metabolism ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; Mice ; Mice, Nude ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Procollagen-Proline Dioxygenase - biosynthesis ; Procollagen-Proline Dioxygenase - genetics ; Procollagen-Proline Dioxygenase - metabolism ; Prostatic Neoplasms - enzymology ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - pathology ; Research Paper ; RNA, Small Interfering - administration & dosage ; RNA, Small Interfering - genetics ; Transfection</subject><ispartof>Oncotarget, 2014-08, Vol.5 (16), p.6654-6669</ispartof><rights>Copyright: © 2014 Chakravarthi et al. 2014</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196154/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196154/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25115393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chakravarthi, Balabhadrapatruni V S K</creatorcontrib><creatorcontrib>Pathi, Satya Sreehari</creatorcontrib><creatorcontrib>Goswami, Moloy T</creatorcontrib><creatorcontrib>Cieślik, Marcin</creatorcontrib><creatorcontrib>Zheng, Heng</creatorcontrib><creatorcontrib>Nallasivam, Sivakumar</creatorcontrib><creatorcontrib>Arekapudi, Subramanyeswara R</creatorcontrib><creatorcontrib>Jing, Xiaojun</creatorcontrib><creatorcontrib>Siddiqui, Javed</creatorcontrib><creatorcontrib>Athanikar, Jyoti</creatorcontrib><creatorcontrib>Carskadon, Shannon L</creatorcontrib><creatorcontrib>Lonigro, Robert J</creatorcontrib><creatorcontrib>Kunju, Lakshmi P</creatorcontrib><creatorcontrib>Chinnaiyan, Arul M</creatorcontrib><creatorcontrib>Palanisamy, Nallasivam</creatorcontrib><creatorcontrib>Varambally, Sooryanarayana</creatorcontrib><title>The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression</title><title>Oncotarget</title><addtitle>Oncotarget</addtitle><description>Collagen prolyl hydroxylases (C-P4HAs) are a family of enzymes involved in collagen biogenesis. One of the isoforms of P4HA, Prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), catalyzes the formation of 4-hydroxyproline that is essential for the proper three-dimensional folding of newly synthesized procollagen chains. Here, we show the overexpression of P4HA1 in aggressive prostate cancer. Immunohistochemical analysis using tissue microarray demonstrated that P4HA1 expression was correlated with prostate cancer progression. Using in vitro studies, we showed that P4HA1 plays a critical role in prostate cancer cell growth and tumor progression. Expression profiling studies using P4HA1 modulated prostate cells suggested regulation of Matrix metalloproteases 1. The invasive properties of P4HA1 overexpressing cells were reversed by blocking MMP1. Our studies indicate P4HA1 copy number gain in a subset of metastatic prostate tumors and its expression is also regulated by microRNA-124. MiR-124 in turn is negatively regulated by transcriptional repressors EZH2 and CtBP1, both of which are overexpressed in aggressive prostate cancer. Chick chorioallantoic membrane (CAM) assay and mice xenograft investigations show that P4HA1 is required for tumor growth and metastasis in vivo. Our observations suggest that P4HA1 plays a critical role in prostate cancer progression and could serve as a viable therapeutic target.</description><subject>Animals</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - physiology</subject><subject>Disease Progression</subject><subject>Gene Expression</subject><subject>HEK293 Cells</subject><subject>Heterografts</subject><subject>Humans</subject><subject>Male</subject><subject>Matrix Metalloproteinase 1 - biosynthesis</subject><subject>Matrix Metalloproteinase 1 - genetics</subject><subject>Matrix Metalloproteinase 1 - metabolism</subject><subject>Membrane Proteins - biosynthesis</subject><subject>Membrane Proteins - genetics</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Procollagen-Proline Dioxygenase - biosynthesis</subject><subject>Procollagen-Proline Dioxygenase - genetics</subject><subject>Procollagen-Proline Dioxygenase - metabolism</subject><subject>Prostatic Neoplasms - enzymology</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Research Paper</subject><subject>RNA, Small Interfering - administration & dosage</subject><subject>RNA, Small Interfering - genetics</subject><subject>Transfection</subject><issn>1949-2553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkE1Lw0AQhhdBbKm9e5L9A1uzn8lehFLUCi0Wqecw2WzSlW0SdqM0_96IH-i8h5dheJ7DIHRFkwXNFGc3bWPaHkJt-wVjSXaGplQLTZiUfILmMb4m40iRZkxfoAmTlEqu-RSZ_cHio3smlAnShdYPHh-GMrSnwUO0eCfWS0q22x3FcHIRdx6GiAGb4HpnwOMRsdg1eGRjD73FBhpjw-deBxuja5tLdF6Bj3b-3TP0cn-3X63J5unhcbXckI4q3hOhUmZSaVRCVWKNgLJIUlMWShRGa6XkGCqYNBlUuso4q4AnSnAprE6VUnyGbr-83VtxtKWxTR_A511wRwhD3oLL_18ad8jr9j0XVCsqxSi4_iv4JX_exT8A9OFtsA</recordid><startdate>20140830</startdate><enddate>20140830</enddate><creator>Chakravarthi, Balabhadrapatruni V S K</creator><creator>Pathi, Satya Sreehari</creator><creator>Goswami, Moloy T</creator><creator>Cieślik, Marcin</creator><creator>Zheng, Heng</creator><creator>Nallasivam, Sivakumar</creator><creator>Arekapudi, Subramanyeswara R</creator><creator>Jing, Xiaojun</creator><creator>Siddiqui, Javed</creator><creator>Athanikar, Jyoti</creator><creator>Carskadon, Shannon L</creator><creator>Lonigro, Robert J</creator><creator>Kunju, Lakshmi P</creator><creator>Chinnaiyan, Arul M</creator><creator>Palanisamy, Nallasivam</creator><creator>Varambally, Sooryanarayana</creator><general>Impact Journals LLC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>20140830</creationdate><title>The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression</title><author>Chakravarthi, Balabhadrapatruni V S K ; Pathi, Satya Sreehari ; Goswami, Moloy T ; Cieślik, Marcin ; Zheng, Heng ; Nallasivam, Sivakumar ; Arekapudi, Subramanyeswara R ; Jing, Xiaojun ; Siddiqui, Javed ; Athanikar, Jyoti ; Carskadon, Shannon L ; Lonigro, Robert J ; Kunju, Lakshmi P ; Chinnaiyan, Arul M ; Palanisamy, Nallasivam ; Varambally, Sooryanarayana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p163t-4672c75c60160ec4adb07cdb64bc996656561425c8af9f832fa3064354e976663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - physiology</topic><topic>Disease Progression</topic><topic>Gene Expression</topic><topic>HEK293 Cells</topic><topic>Heterografts</topic><topic>Humans</topic><topic>Male</topic><topic>Matrix Metalloproteinase 1 - biosynthesis</topic><topic>Matrix Metalloproteinase 1 - genetics</topic><topic>Matrix Metalloproteinase 1 - metabolism</topic><topic>Membrane Proteins - biosynthesis</topic><topic>Membrane Proteins - genetics</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Procollagen-Proline Dioxygenase - biosynthesis</topic><topic>Procollagen-Proline Dioxygenase - genetics</topic><topic>Procollagen-Proline Dioxygenase - metabolism</topic><topic>Prostatic Neoplasms - enzymology</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Research Paper</topic><topic>RNA, Small Interfering - administration & dosage</topic><topic>RNA, Small Interfering - genetics</topic><topic>Transfection</topic><toplevel>online_resources</toplevel><creatorcontrib>Chakravarthi, Balabhadrapatruni V S K</creatorcontrib><creatorcontrib>Pathi, Satya Sreehari</creatorcontrib><creatorcontrib>Goswami, Moloy T</creatorcontrib><creatorcontrib>Cieślik, Marcin</creatorcontrib><creatorcontrib>Zheng, Heng</creatorcontrib><creatorcontrib>Nallasivam, Sivakumar</creatorcontrib><creatorcontrib>Arekapudi, Subramanyeswara R</creatorcontrib><creatorcontrib>Jing, Xiaojun</creatorcontrib><creatorcontrib>Siddiqui, Javed</creatorcontrib><creatorcontrib>Athanikar, Jyoti</creatorcontrib><creatorcontrib>Carskadon, Shannon L</creatorcontrib><creatorcontrib>Lonigro, Robert J</creatorcontrib><creatorcontrib>Kunju, Lakshmi P</creatorcontrib><creatorcontrib>Chinnaiyan, Arul M</creatorcontrib><creatorcontrib>Palanisamy, Nallasivam</creatorcontrib><creatorcontrib>Varambally, Sooryanarayana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Oncotarget</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chakravarthi, Balabhadrapatruni V S K</au><au>Pathi, Satya Sreehari</au><au>Goswami, Moloy T</au><au>Cieślik, Marcin</au><au>Zheng, Heng</au><au>Nallasivam, Sivakumar</au><au>Arekapudi, Subramanyeswara R</au><au>Jing, Xiaojun</au><au>Siddiqui, Javed</au><au>Athanikar, Jyoti</au><au>Carskadon, Shannon L</au><au>Lonigro, Robert J</au><au>Kunju, Lakshmi P</au><au>Chinnaiyan, Arul M</au><au>Palanisamy, Nallasivam</au><au>Varambally, Sooryanarayana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression</atitle><jtitle>Oncotarget</jtitle><addtitle>Oncotarget</addtitle><date>2014-08-30</date><risdate>2014</risdate><volume>5</volume><issue>16</issue><spage>6654</spage><epage>6669</epage><pages>6654-6669</pages><eissn>1949-2553</eissn><abstract>Collagen prolyl hydroxylases (C-P4HAs) are a family of enzymes involved in collagen biogenesis. One of the isoforms of P4HA, Prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), catalyzes the formation of 4-hydroxyproline that is essential for the proper three-dimensional folding of newly synthesized procollagen chains. Here, we show the overexpression of P4HA1 in aggressive prostate cancer. Immunohistochemical analysis using tissue microarray demonstrated that P4HA1 expression was correlated with prostate cancer progression. Using in vitro studies, we showed that P4HA1 plays a critical role in prostate cancer cell growth and tumor progression. Expression profiling studies using P4HA1 modulated prostate cells suggested regulation of Matrix metalloproteases 1. The invasive properties of P4HA1 overexpressing cells were reversed by blocking MMP1. Our studies indicate P4HA1 copy number gain in a subset of metastatic prostate tumors and its expression is also regulated by microRNA-124. MiR-124 in turn is negatively regulated by transcriptional repressors EZH2 and CtBP1, both of which are overexpressed in aggressive prostate cancer. Chick chorioallantoic membrane (CAM) assay and mice xenograft investigations show that P4HA1 is required for tumor growth and metastasis in vivo. Our observations suggest that P4HA1 plays a critical role in prostate cancer progression and could serve as a viable therapeutic target.</abstract><cop>United States</cop><pub>Impact Journals LLC</pub><pmid>25115393</pmid><doi>10.18632/oncotarget.2208</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Line, Tumor Cell Proliferation - physiology Disease Progression Gene Expression HEK293 Cells Heterografts Humans Male Matrix Metalloproteinase 1 - biosynthesis Matrix Metalloproteinase 1 - genetics Matrix Metalloproteinase 1 - metabolism Membrane Proteins - biosynthesis Membrane Proteins - genetics Mice Mice, Nude MicroRNAs - genetics MicroRNAs - metabolism Procollagen-Proline Dioxygenase - biosynthesis Procollagen-Proline Dioxygenase - genetics Procollagen-Proline Dioxygenase - metabolism Prostatic Neoplasms - enzymology Prostatic Neoplasms - genetics Prostatic Neoplasms - pathology Research Paper RNA, Small Interfering - administration & dosage RNA, Small Interfering - genetics Transfection |
| title | The miR-124-prolyl hydroxylase P4HA1-MMP1 axis plays a critical role in prostate cancer progression |
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