The novel macrolide-Lincosamide-Streptogramin B resistance gene erm(44) is associated with a prophage in Staphylococcus xylosus
A novel erythromycin ribosome methylase gene, erm(44), that confers resistance to macrolide, lincosamide, and streptogramin B (MLSB) antibiotics was identified by whole-genome sequencing of the chromosome of Staphylococcus xylosus isolated from bovine mastitis milk. The erm(44) gene is preceded by a...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2014-10, Vol.58 (10), p.6133-6138 |
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| creator | Wipf, Juliette R K Schwendener, Sybille Perreten, Vincent |
| description | A novel erythromycin ribosome methylase gene, erm(44), that confers resistance to macrolide, lincosamide, and streptogramin B (MLSB) antibiotics was identified by whole-genome sequencing of the chromosome of Staphylococcus xylosus isolated from bovine mastitis milk. The erm(44) gene is preceded by a regulatory sequence that encodes two leader peptides responsible for the inducible expression of the methylase gene, as demonstrated by cloning in Staphylococcus aureus. The erm(44) gene is located on a 53-kb putative prophage designated ΦJW4341-pro. The 56 predicted open reading frames of ΦJW4341-pro are structurally organized into the five functional modules found in members of the family Siphoviridae. ΦJW4341-pro is site-specifically integrated into the S. xylosus chromosome, where it is flanked by two perfect 19-bp direct repeats, and exhibits the ability to circularize. The presence of erm(44) in three additional S. xylosus strains suggests that this putative prophage has the potential to disseminate MLSB resistance. |
| doi_str_mv | 10.1128/AAC.02949-14 |
| format | Article |
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The erm(44) gene is preceded by a regulatory sequence that encodes two leader peptides responsible for the inducible expression of the methylase gene, as demonstrated by cloning in Staphylococcus aureus. The erm(44) gene is located on a 53-kb putative prophage designated ΦJW4341-pro. The 56 predicted open reading frames of ΦJW4341-pro are structurally organized into the five functional modules found in members of the family Siphoviridae. ΦJW4341-pro is site-specifically integrated into the S. xylosus chromosome, where it is flanked by two perfect 19-bp direct repeats, and exhibits the ability to circularize. The presence of erm(44) in three additional S. xylosus strains suggests that this putative prophage has the potential to disseminate MLSB resistance.</description><identifier>ISSN: 0066-4804</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AAC.02949-14</identifier><identifier>PMID: 25092709</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Anti-Bacterial Agents ; Anti-Bacterial Agents - pharmacology ; Drug Resistance, Multiple, Bacterial - genetics ; Lincosamides ; Lincosamides - pharmacology ; Macrolides ; Macrolides - pharmacology ; Mechanisms of Resistance ; Open Reading Frames - genetics ; Prophages ; Prophages - genetics ; Staphylococcus ; Staphylococcus - drug effects ; Staphylococcus - genetics ; Staphylococcus - virology ; Staphylococcus aureus ; Staphylococcus aureus - genetics ; Staphylococcus xylosus ; Streptogramin B ; Streptogramin B - pharmacology</subject><ispartof>Antimicrobial agents and chemotherapy, 2014-10, Vol.58 (10), p.6133-6138</ispartof><rights>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2014, American Society for Microbiology. All Rights Reserved. 2014 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a451t-741ce7baec8c02f6342ae4fdb6ed179f4218cf6e869cee34d32071b9eccb47de3</citedby><cites>FETCH-LOGICAL-a451t-741ce7baec8c02f6342ae4fdb6ed179f4218cf6e869cee34d32071b9eccb47de3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187952/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187952/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25092709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wipf, Juliette R K</creatorcontrib><creatorcontrib>Schwendener, Sybille</creatorcontrib><creatorcontrib>Perreten, Vincent</creatorcontrib><title>The novel macrolide-Lincosamide-Streptogramin B resistance gene erm(44) is associated with a prophage in Staphylococcus xylosus</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>A novel erythromycin ribosome methylase gene, erm(44), that confers resistance to macrolide, lincosamide, and streptogramin B (MLSB) antibiotics was identified by whole-genome sequencing of the chromosome of Staphylococcus xylosus isolated from bovine mastitis milk. The erm(44) gene is preceded by a regulatory sequence that encodes two leader peptides responsible for the inducible expression of the methylase gene, as demonstrated by cloning in Staphylococcus aureus. The erm(44) gene is located on a 53-kb putative prophage designated ΦJW4341-pro. The 56 predicted open reading frames of ΦJW4341-pro are structurally organized into the five functional modules found in members of the family Siphoviridae. ΦJW4341-pro is site-specifically integrated into the S. xylosus chromosome, where it is flanked by two perfect 19-bp direct repeats, and exhibits the ability to circularize. The presence of erm(44) in three additional S. xylosus strains suggests that this putative prophage has the potential to disseminate MLSB resistance.</description><subject>Anti-Bacterial Agents</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Lincosamides</subject><subject>Lincosamides - pharmacology</subject><subject>Macrolides</subject><subject>Macrolides - pharmacology</subject><subject>Mechanisms of Resistance</subject><subject>Open Reading Frames - genetics</subject><subject>Prophages</subject><subject>Prophages - genetics</subject><subject>Staphylococcus</subject><subject>Staphylococcus - drug effects</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - virology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus xylosus</subject><subject>Streptogramin B</subject><subject>Streptogramin B - pharmacology</subject><issn>0066-4804</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0Eokvhxhn52Eqk2I7jxBekZQUUaaUeWs6W40w2rhI72E5LT_x1vGypyqHiNDOab55m5iH0lpIzSlnzYb3enBEmuSwof4ZWlMimEJUUz9GKECEK3hB-hF7FeE1yXUnyEh2xikhWE7lCv64GwM7fwIgnbYIfbQfF1jrjo572-WUKMCe_C7l0-BMOEG1M2hnAO3CAIUwnnJ9iG7GO0RurE3T41qYBazwHPw96BziPXiY9D3ejN96YJeKfOY1LfI1e9HqM8OY-HqPvXz5fbc6L7cXXb5v1ttC8oqmoOTVQtxpMYwjrRcmZBt53rYCO1rLnjDamF9AIaQBK3pWM1LSVYEzL6w7KY_TxoDsv7QSdAZeCHtUc7KTDnfLaqn87zg5q528Up00tK5YFTu4Fgv-xQExqstHAOGoHfomKCsYEIZyJ_6OVYELwku7R9wc0vz7GAP3DRpSovb0q26v-2Ksoz_jpAddxYuraL8Hlpz3Fvnt88YPwX-_L37PisAA</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Wipf, Juliette R K</creator><creator>Schwendener, Sybille</creator><creator>Perreten, Vincent</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>20141001</creationdate><title>The novel macrolide-Lincosamide-Streptogramin B resistance gene erm(44) is associated with a prophage in Staphylococcus xylosus</title><author>Wipf, Juliette R K ; Schwendener, Sybille ; Perreten, Vincent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a451t-741ce7baec8c02f6342ae4fdb6ed179f4218cf6e869cee34d32071b9eccb47de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-Bacterial Agents</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Lincosamides</topic><topic>Lincosamides - pharmacology</topic><topic>Macrolides</topic><topic>Macrolides - pharmacology</topic><topic>Mechanisms of Resistance</topic><topic>Open Reading Frames - genetics</topic><topic>Prophages</topic><topic>Prophages - genetics</topic><topic>Staphylococcus</topic><topic>Staphylococcus - drug effects</topic><topic>Staphylococcus - genetics</topic><topic>Staphylococcus - virology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus xylosus</topic><topic>Streptogramin B</topic><topic>Streptogramin B - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wipf, Juliette R K</creatorcontrib><creatorcontrib>Schwendener, Sybille</creatorcontrib><creatorcontrib>Perreten, Vincent</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial agents and chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wipf, Juliette R K</au><au>Schwendener, Sybille</au><au>Perreten, Vincent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The novel macrolide-Lincosamide-Streptogramin B resistance gene erm(44) is associated with a prophage in Staphylococcus xylosus</atitle><jtitle>Antimicrobial agents and chemotherapy</jtitle><stitle>Antimicrob Agents Chemother</stitle><addtitle>Antimicrob Agents Chemother</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>58</volume><issue>10</issue><spage>6133</spage><epage>6138</epage><pages>6133-6138</pages><issn>0066-4804</issn><eissn>1098-6596</eissn><abstract>A novel erythromycin ribosome methylase gene, erm(44), that confers resistance to macrolide, lincosamide, and streptogramin B (MLSB) antibiotics was identified by whole-genome sequencing of the chromosome of Staphylococcus xylosus isolated from bovine mastitis milk. The erm(44) gene is preceded by a regulatory sequence that encodes two leader peptides responsible for the inducible expression of the methylase gene, as demonstrated by cloning in Staphylococcus aureus. The erm(44) gene is located on a 53-kb putative prophage designated ΦJW4341-pro. The 56 predicted open reading frames of ΦJW4341-pro are structurally organized into the five functional modules found in members of the family Siphoviridae. ΦJW4341-pro is site-specifically integrated into the S. xylosus chromosome, where it is flanked by two perfect 19-bp direct repeats, and exhibits the ability to circularize. The presence of erm(44) in three additional S. xylosus strains suggests that this putative prophage has the potential to disseminate MLSB resistance.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>25092709</pmid><doi>10.1128/AAC.02949-14</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Anti-Bacterial Agents Anti-Bacterial Agents - pharmacology Drug Resistance, Multiple, Bacterial - genetics Lincosamides Lincosamides - pharmacology Macrolides Macrolides - pharmacology Mechanisms of Resistance Open Reading Frames - genetics Prophages Prophages - genetics Staphylococcus Staphylococcus - drug effects Staphylococcus - genetics Staphylococcus - virology Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus xylosus Streptogramin B Streptogramin B - pharmacology |
| title | The novel macrolide-Lincosamide-Streptogramin B resistance gene erm(44) is associated with a prophage in Staphylococcus xylosus |
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