Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers
The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, an...
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| Veröffentlicht in: | Journal of the American Chemical Society 2014-10, Vol.136 (39), p.13709-13714 |
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| container_end_page | 13714 |
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| container_issue | 39 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 136 |
| creator | Warren, Andrew D Gaylord, Shonda T Ngan, Kevin C Dumont Milutinovic, Milena Kwong, Gabriel A Bhatia, Sangeeta N Walt, David R |
| description | The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for “microdosing.” |
| doi_str_mv | 10.1021/ja505676h |
| format | Article |
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| identifier | ISSN: 0002-7863 |
| ispartof | Journal of the American Chemical Society, 2014-10, Vol.136 (39), p.13709-13714 |
| issn | 0002-7863 1520-5126 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Biomarkers Biomarkers - urine Customizing Diagnostic systems Dose-Response Relationship, Drug Female Guidelines Mice Monitoring Nanoparticles Nanoparticles - administration & dosage Nanoparticles - chemistry Regulators Thrombin Thrombin - administration & dosage Thrombin - chemistry Venous Thromboembolism - diagnosis |
| title | Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers |
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