Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans
Background and Objective: This study analyzed the interaction of Porphyromonas gingivalis ATCC 33277 and Aggregatibacter actinomycetemcomitans Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis. Material and Methods: P...
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| Veröffentlicht in: | Journal of periodontal research 2009-06, Vol.44 (3), p.368-377 |
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| creator | Guentsch, A. Puklo, M. Preshaw, P. M. Glockmann, E. Pfister, W. Potempa, J. Eick, S. |
| description | Background and Objective: This study analyzed the interaction of Porphyromonas gingivalis ATCC 33277 and Aggregatibacter actinomycetemcomitans Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis.
Material and Methods: Peripheral blood polymorphonuclear neutrophils obtained from 12 patients with chronic periodontitis, six patients with aggressive periodontitis and 12 healthy controls were exposed to P. gingivalis and A. actinomycetemcomitans following opsonization of the bacteria using the patient’s own serum. Serum immunoglobulin G (IgG) levels against both periodontopathogens were measured. Phagocytosis and killing of the bacteria, as well as the extracellular human neutrophil elastase activity, were quantified. The total amount and the extracellular release of reactive oxygen species were measured using luminol‐dependent and isoluminol‐dependent chemiluminescence.
Results: Polymorphonuclear neutrophils from patients with chronic (62.16 ± 19.39%) and aggressive (43.26 ± 26.63%) periodontitis phagocytosed more P. gingivalis than the healthy controls (24.43 ± 19.87%) at the 30‐min time point after exposure to the bacteria (p |
| doi_str_mv | 10.1111/j.1600-0765.2008.01113.x |
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Material and Methods: Peripheral blood polymorphonuclear neutrophils obtained from 12 patients with chronic periodontitis, six patients with aggressive periodontitis and 12 healthy controls were exposed to P. gingivalis and A. actinomycetemcomitans following opsonization of the bacteria using the patient’s own serum. Serum immunoglobulin G (IgG) levels against both periodontopathogens were measured. Phagocytosis and killing of the bacteria, as well as the extracellular human neutrophil elastase activity, were quantified. The total amount and the extracellular release of reactive oxygen species were measured using luminol‐dependent and isoluminol‐dependent chemiluminescence.
Results: Polymorphonuclear neutrophils from patients with chronic (62.16 ± 19.39%) and aggressive (43.26 ± 26.63%) periodontitis phagocytosed more P. gingivalis than the healthy controls (24.43 ± 19.87%) at the 30‐min time point after exposure to the bacteria (p < 0.05). High serum IgG levels against P. gingivalis and A. actinomycetemcomitans were detected in subjects with periodontitis. Polymorphonuclear neutrophils from subjects with chronic and aggressive periodontitis released significantly more reactive oxygen species and demonstrated greater human neutrophil elastase activity in the absence of any stimulus than polymorphonuclear neutrophils from healthy controls (p < 0.05). Polymorphonuclear neutrophils in chronic periodontitis released significantly more reactive oxygen species when exposed to P. gingivalis and A. actinomycetemcomitans than polymorphonuclear neutrophils in aggressive periodontitis.
Conclusion: High serum IgG levels against P. gingivalis and A. actinomycetemcomitans promote phagocytosis in periodontitis. The extracellular release of reactive oxygen species and neutrophil elastase by polymorphonuclear neutrophils may also contribute to damage of the surrounding periodontal tissues.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2008.01113.x</identifier><identifier>PMID: 19210340</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aggregatibacter actinomycetemcomitans ; Aggregatibacter actinomycetemcomitans - immunology ; Aggressive Periodontitis - blood ; Aggressive Periodontitis - immunology ; Aggressive Periodontitis - microbiology ; Antibodies, Bacterial - immunology ; Biological and medical sciences ; Case-Control Studies ; Chronic Periodontitis - blood ; Chronic Periodontitis - immunology ; Chronic Periodontitis - microbiology ; Dentistry ; Facial bones, jaws, teeth, parodontium: diseases, semeiology ; Female ; Humans ; Immunoglobulin G - immunology ; Leukocyte Elastase - blood ; Leukocyte Elastase - metabolism ; Luminescence ; Male ; Medical sciences ; Middle Aged ; Neutrophils - immunology ; Non tumoral diseases ; Otorhinolaryngology. Stomatology ; periodontitis ; Phagocytosis ; polymorphonuclear neutrophil ; Porphyromonas gingivalis ; Porphyromonas gingivalis - immunology ; reactive oxygen species ; Reactive Oxygen Species - metabolism</subject><ispartof>Journal of periodontal research, 2009-06, Vol.44 (3), p.368-377</ispartof><rights>2009 The Authors. Journal compilation © 2009 Blackwell Munksgaard</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5713-fc444b49e7e2bfa7089d3b81ec23088097a5a6e061b9ef448f009697ac97ea8f3</citedby><cites>FETCH-LOGICAL-c5713-fc444b49e7e2bfa7089d3b81ec23088097a5a6e061b9ef448f009697ac97ea8f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0765.2008.01113.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0765.2008.01113.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21385927$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19210340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guentsch, A.</creatorcontrib><creatorcontrib>Puklo, M.</creatorcontrib><creatorcontrib>Preshaw, P. M.</creatorcontrib><creatorcontrib>Glockmann, E.</creatorcontrib><creatorcontrib>Pfister, W.</creatorcontrib><creatorcontrib>Potempa, J.</creatorcontrib><creatorcontrib>Eick, S.</creatorcontrib><title>Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objective: This study analyzed the interaction of Porphyromonas gingivalis ATCC 33277 and Aggregatibacter actinomycetemcomitans Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis.
Material and Methods: Peripheral blood polymorphonuclear neutrophils obtained from 12 patients with chronic periodontitis, six patients with aggressive periodontitis and 12 healthy controls were exposed to P. gingivalis and A. actinomycetemcomitans following opsonization of the bacteria using the patient’s own serum. Serum immunoglobulin G (IgG) levels against both periodontopathogens were measured. Phagocytosis and killing of the bacteria, as well as the extracellular human neutrophil elastase activity, were quantified. The total amount and the extracellular release of reactive oxygen species were measured using luminol‐dependent and isoluminol‐dependent chemiluminescence.
Results: Polymorphonuclear neutrophils from patients with chronic (62.16 ± 19.39%) and aggressive (43.26 ± 26.63%) periodontitis phagocytosed more P. gingivalis than the healthy controls (24.43 ± 19.87%) at the 30‐min time point after exposure to the bacteria (p < 0.05). High serum IgG levels against P. gingivalis and A. actinomycetemcomitans were detected in subjects with periodontitis. Polymorphonuclear neutrophils from subjects with chronic and aggressive periodontitis released significantly more reactive oxygen species and demonstrated greater human neutrophil elastase activity in the absence of any stimulus than polymorphonuclear neutrophils from healthy controls (p < 0.05). Polymorphonuclear neutrophils in chronic periodontitis released significantly more reactive oxygen species when exposed to P. gingivalis and A. actinomycetemcomitans than polymorphonuclear neutrophils in aggressive periodontitis.
Conclusion: High serum IgG levels against P. gingivalis and A. actinomycetemcomitans promote phagocytosis in periodontitis. The extracellular release of reactive oxygen species and neutrophil elastase by polymorphonuclear neutrophils may also contribute to damage of the surrounding periodontal tissues.</description><subject>Adult</subject><subject>Aggregatibacter actinomycetemcomitans</subject><subject>Aggregatibacter actinomycetemcomitans - immunology</subject><subject>Aggressive Periodontitis - blood</subject><subject>Aggressive Periodontitis - immunology</subject><subject>Aggressive Periodontitis - microbiology</subject><subject>Antibodies, Bacterial - immunology</subject><subject>Biological and medical sciences</subject><subject>Case-Control Studies</subject><subject>Chronic Periodontitis - blood</subject><subject>Chronic Periodontitis - immunology</subject><subject>Chronic Periodontitis - microbiology</subject><subject>Dentistry</subject><subject>Facial bones, jaws, teeth, parodontium: diseases, semeiology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin G - immunology</subject><subject>Leukocyte Elastase - blood</subject><subject>Leukocyte Elastase - metabolism</subject><subject>Luminescence</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neutrophils - immunology</subject><subject>Non tumoral diseases</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>periodontitis</subject><subject>Phagocytosis</subject><subject>polymorphonuclear neutrophil</subject><subject>Porphyromonas gingivalis</subject><subject>Porphyromonas gingivalis - immunology</subject><subject>reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhSMEokPhFVA2sEvwXxJnAVJVlQKqCqqAsrMcz03iIbGD7ZnOvAJPjdMZDbACb2zf-52ja58kSTHKcVyvVjkuEcpQVRY5QYjnKFZpvn2QLI6Nh8kCIUIyyjg7SZ54v0LxXlb14-QE1wQjytAi-XkN6-Ds1OvBp9qkqnfWaJVKs0xl1znwXm8gncBpu7Qm6KDvOW0COKmCtia906FPP1k39TtnR2ukTzttOr2RQ4Rnp7PZqZNBN1ECLp2Fxo47BQFGZUcdpPFPk0etHDw8O-ynyZe3F5_P32VXHy_fn59dZaqoMM1axRhrWA0VkKaVFeL1kjYcgyIUcY7qShayBFTipoaWMd4iVJexquoKJG_pafJm7zutmxGWCkxwchCT06N0O2GlFn93jO5FZzeCYR6teDR4eTBw9scafBCj9gqGQRqway_KihQxAPxPkKCCkZLTCPI9qJz13kF7nAYjMScuVmIOVszBijlxcZ-42Ebp8z9f81t4iDgCLw6A9EoOrZNGaX_kCKa8qEkVudd77k4PsPvvAcSHm4v5FPXZXq99gO1RL933-CG0KsTt9aWgN9_YV8pvBaa_APSd21s</recordid><startdate>200906</startdate><enddate>200906</enddate><creator>Guentsch, A.</creator><creator>Puklo, M.</creator><creator>Preshaw, P. M.</creator><creator>Glockmann, E.</creator><creator>Pfister, W.</creator><creator>Potempa, J.</creator><creator>Eick, S.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200906</creationdate><title>Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans</title><author>Guentsch, A. ; Puklo, M. ; Preshaw, P. M. ; Glockmann, E. ; Pfister, W. ; Potempa, J. ; Eick, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5713-fc444b49e7e2bfa7089d3b81ec23088097a5a6e061b9ef448f009697ac97ea8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adult</topic><topic>Aggregatibacter actinomycetemcomitans</topic><topic>Aggregatibacter actinomycetemcomitans - immunology</topic><topic>Aggressive Periodontitis - blood</topic><topic>Aggressive Periodontitis - immunology</topic><topic>Aggressive Periodontitis - microbiology</topic><topic>Antibodies, Bacterial - immunology</topic><topic>Biological and medical sciences</topic><topic>Case-Control Studies</topic><topic>Chronic Periodontitis - blood</topic><topic>Chronic Periodontitis - immunology</topic><topic>Chronic Periodontitis - microbiology</topic><topic>Dentistry</topic><topic>Facial bones, jaws, teeth, parodontium: diseases, semeiology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoglobulin G - immunology</topic><topic>Leukocyte Elastase - blood</topic><topic>Leukocyte Elastase - metabolism</topic><topic>Luminescence</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neutrophils - immunology</topic><topic>Non tumoral diseases</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>periodontitis</topic><topic>Phagocytosis</topic><topic>polymorphonuclear neutrophil</topic><topic>Porphyromonas gingivalis</topic><topic>Porphyromonas gingivalis - immunology</topic><topic>reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guentsch, A.</creatorcontrib><creatorcontrib>Puklo, M.</creatorcontrib><creatorcontrib>Preshaw, P. M.</creatorcontrib><creatorcontrib>Glockmann, E.</creatorcontrib><creatorcontrib>Pfister, W.</creatorcontrib><creatorcontrib>Potempa, J.</creatorcontrib><creatorcontrib>Eick, S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guentsch, A.</au><au>Puklo, M.</au><au>Preshaw, P. M.</au><au>Glockmann, E.</au><au>Pfister, W.</au><au>Potempa, J.</au><au>Eick, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2009-06</date><risdate>2009</risdate><volume>44</volume><issue>3</issue><spage>368</spage><epage>377</epage><pages>368-377</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective: This study analyzed the interaction of Porphyromonas gingivalis ATCC 33277 and Aggregatibacter actinomycetemcomitans Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis.
Material and Methods: Peripheral blood polymorphonuclear neutrophils obtained from 12 patients with chronic periodontitis, six patients with aggressive periodontitis and 12 healthy controls were exposed to P. gingivalis and A. actinomycetemcomitans following opsonization of the bacteria using the patient’s own serum. Serum immunoglobulin G (IgG) levels against both periodontopathogens were measured. Phagocytosis and killing of the bacteria, as well as the extracellular human neutrophil elastase activity, were quantified. The total amount and the extracellular release of reactive oxygen species were measured using luminol‐dependent and isoluminol‐dependent chemiluminescence.
Results: Polymorphonuclear neutrophils from patients with chronic (62.16 ± 19.39%) and aggressive (43.26 ± 26.63%) periodontitis phagocytosed more P. gingivalis than the healthy controls (24.43 ± 19.87%) at the 30‐min time point after exposure to the bacteria (p < 0.05). High serum IgG levels against P. gingivalis and A. actinomycetemcomitans were detected in subjects with periodontitis. Polymorphonuclear neutrophils from subjects with chronic and aggressive periodontitis released significantly more reactive oxygen species and demonstrated greater human neutrophil elastase activity in the absence of any stimulus than polymorphonuclear neutrophils from healthy controls (p < 0.05). Polymorphonuclear neutrophils in chronic periodontitis released significantly more reactive oxygen species when exposed to P. gingivalis and A. actinomycetemcomitans than polymorphonuclear neutrophils in aggressive periodontitis.
Conclusion: High serum IgG levels against P. gingivalis and A. actinomycetemcomitans promote phagocytosis in periodontitis. The extracellular release of reactive oxygen species and neutrophil elastase by polymorphonuclear neutrophils may also contribute to damage of the surrounding periodontal tissues.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19210340</pmid><doi>10.1111/j.1600-0765.2008.01113.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aggregatibacter actinomycetemcomitans Aggregatibacter actinomycetemcomitans - immunology Aggressive Periodontitis - blood Aggressive Periodontitis - immunology Aggressive Periodontitis - microbiology Antibodies, Bacterial - immunology Biological and medical sciences Case-Control Studies Chronic Periodontitis - blood Chronic Periodontitis - immunology Chronic Periodontitis - microbiology Dentistry Facial bones, jaws, teeth, parodontium: diseases, semeiology Female Humans Immunoglobulin G - immunology Leukocyte Elastase - blood Leukocyte Elastase - metabolism Luminescence Male Medical sciences Middle Aged Neutrophils - immunology Non tumoral diseases Otorhinolaryngology. Stomatology periodontitis Phagocytosis polymorphonuclear neutrophil Porphyromonas gingivalis Porphyromonas gingivalis - immunology reactive oxygen species Reactive Oxygen Species - metabolism |
| title | Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans |
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