Efficient in vivo deletion of a large imprinted lncRNA by CRISPR/Cas9

Recent genome-wide studies have revealed that the majority of the mouse genome is transcribed as non-coding RNAs (ncRNAs) and growing evidence supports the importance of ncRNAs in regulating gene expression and epigenetic processes. However, the low efficiency of conventional gene targeting strategi...

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Veröffentlicht in:	RNA biology 2014-07, Vol.11 (7), p.829-835
Hauptverfasser:	Han, Jinxiong, Zhang, Jun, Chen, Li, Shen, Bin, Zhou, Jiankui, Hu, Bian, Du, Yinan, Tate, Peri H, Huang, Xingxu, Zhang, Wensheng
Format:	Artikel
Sprache:	eng
Schlagworte:	alleles Animals Chromosomes, Mammalian - genetics CRISPR-Cas Systems CRISPR/Cas9 engineering epigenetics Gene Deletion gene editing gene expression gene targeting Gene Targeting - economics Gene Targeting - methods Genomic Imprinting genomics imprinting inheritance (genetics) large fragment deletion lncRNA loci Mice mutation non-coding RNA Nuclear Proteins - genetics Nuclear Proteins - metabolism Rian somatic cells Technical Paper transcription (genetics)
Online-Zugang:	Volltext
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