Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages
In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterizat...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2014-09, Vol.80 (18), p.5623-5635 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5635 |
|---|---|
| container_issue | 18 |
| container_start_page | 5623 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 80 |
| creator | Casey, Eoghan Mahony, Jennifer O'Connell-Motherway, Mary Bottacini, Francesca Cornelissen, Anneleen Neve, Horst Heller, Knut J Noben, Jean-Paul Dal Bello, Fabio van Sinderen, Douwe |
| description | In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations. |
| doi_str_mv | 10.1128/AEM.01268-14 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4178594</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3417500821</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-bc930e03b7d0e1d5ebf04df5608447c3df7b545047d7001d07877a2569a923b53</originalsourceid><addsrcrecordid>eNpdkc1v1DAQxS1ERZfCjTOKxIUDWcZfsXNBqqryIW3FBbhatjPZdfHGix1Xgr--KS0V7WmkeT-9madHyCsKa0qZfn96frEGyjrdUvGErCj0upWcd0_JCqDvW8YEHJPnpVwCgIBOPyPHTAIwwemK_LhIEX2NNjd-Z7P1M-bwx84hTU0am3mXEZvNsk7O-hBjLc2A0eWK_mcITamuHNaNq3Frc_CLetjZLZYX5Gi0seDLu3lCvn88_3b2ud18_fTl7HTTeiHk3Drfc0DgTg2AdJDoRhDDKDvQQijPh1E5KSQINSgAOoDSSlkmu972jDvJT8iHW99DdXscPE5zttEcctjb_NskG8xDZQo7s01XRlClZS8Wg7d3Bjn9qlhmsw_FY4x2wlSLobLrtOCSqQV98wi9TDVPS7yFkppTqVW_UO9uKZ9TKRnH-2comJvCzFKY-VuYoTf3X_8f4B7-1xC_Bq_4kWY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1558315879</pqid></control><display><type>article</type><title>Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Casey, Eoghan ; Mahony, Jennifer ; O'Connell-Motherway, Mary ; Bottacini, Francesca ; Cornelissen, Anneleen ; Neve, Horst ; Heller, Knut J ; Noben, Jean-Paul ; Dal Bello, Fabio ; van Sinderen, Douwe</creator><contributor>Björkroth, J.</contributor><creatorcontrib>Casey, Eoghan ; Mahony, Jennifer ; O'Connell-Motherway, Mary ; Bottacini, Francesca ; Cornelissen, Anneleen ; Neve, Horst ; Heller, Knut J ; Noben, Jean-Paul ; Dal Bello, Fabio ; van Sinderen, Douwe ; Björkroth, J.</creatorcontrib><description>In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.01268-14</identifier><identifier>PMID: 25002431</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Bacteriophages - chemistry ; Bacteriophages - genetics ; Bacteriophages - isolation & purification ; Bacteriophages - physiology ; DNA, Viral - genetics ; Fermentation ; Food Microbiology ; Gene Expression Profiling ; Gene Order ; Genetics and Molecular Biology ; Genomes ; Gram-positive bacteria ; Host Specificity ; Lactobacillus delbrueckii ; Lactobacillus delbrueckii - virology ; Mass spectrometry ; Microscopy, Electron, Transmission ; Molecular Sequence Data ; Sequence Analysis, DNA ; Synteny ; Viral Proteins - analysis ; Virion - ultrastructure</subject><ispartof>Applied and Environmental Microbiology, 2014-09, Vol.80 (18), p.5623-5635</ispartof><rights>Copyright © 2014, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright American Society for Microbiology Sep 2014</rights><rights>Copyright © 2014, American Society for Microbiology. All Rights Reserved. 2014 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-bc930e03b7d0e1d5ebf04df5608447c3df7b545047d7001d07877a2569a923b53</citedby><cites>FETCH-LOGICAL-c445t-bc930e03b7d0e1d5ebf04df5608447c3df7b545047d7001d07877a2569a923b53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178594/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4178594/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25002431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Björkroth, J.</contributor><creatorcontrib>Casey, Eoghan</creatorcontrib><creatorcontrib>Mahony, Jennifer</creatorcontrib><creatorcontrib>O'Connell-Motherway, Mary</creatorcontrib><creatorcontrib>Bottacini, Francesca</creatorcontrib><creatorcontrib>Cornelissen, Anneleen</creatorcontrib><creatorcontrib>Neve, Horst</creatorcontrib><creatorcontrib>Heller, Knut J</creatorcontrib><creatorcontrib>Noben, Jean-Paul</creatorcontrib><creatorcontrib>Dal Bello, Fabio</creatorcontrib><creatorcontrib>van Sinderen, Douwe</creatorcontrib><title>Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.</description><subject>Bacteriophages - chemistry</subject><subject>Bacteriophages - genetics</subject><subject>Bacteriophages - isolation & purification</subject><subject>Bacteriophages - physiology</subject><subject>DNA, Viral - genetics</subject><subject>Fermentation</subject><subject>Food Microbiology</subject><subject>Gene Expression Profiling</subject><subject>Gene Order</subject><subject>Genetics and Molecular Biology</subject><subject>Genomes</subject><subject>Gram-positive bacteria</subject><subject>Host Specificity</subject><subject>Lactobacillus delbrueckii</subject><subject>Lactobacillus delbrueckii - virology</subject><subject>Mass spectrometry</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Sequence Data</subject><subject>Sequence Analysis, DNA</subject><subject>Synteny</subject><subject>Viral Proteins - analysis</subject><subject>Virion - ultrastructure</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxS1ERZfCjTOKxIUDWcZfsXNBqqryIW3FBbhatjPZdfHGix1Xgr--KS0V7WmkeT-9madHyCsKa0qZfn96frEGyjrdUvGErCj0upWcd0_JCqDvW8YEHJPnpVwCgIBOPyPHTAIwwemK_LhIEX2NNjd-Z7P1M-bwx84hTU0am3mXEZvNsk7O-hBjLc2A0eWK_mcITamuHNaNq3Frc_CLetjZLZYX5Gi0seDLu3lCvn88_3b2ud18_fTl7HTTeiHk3Drfc0DgTg2AdJDoRhDDKDvQQijPh1E5KSQINSgAOoDSSlkmu972jDvJT8iHW99DdXscPE5zttEcctjb_NskG8xDZQo7s01XRlClZS8Wg7d3Bjn9qlhmsw_FY4x2wlSLobLrtOCSqQV98wi9TDVPS7yFkppTqVW_UO9uKZ9TKRnH-2comJvCzFKY-VuYoTf3X_8f4B7-1xC_Bq_4kWY</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Casey, Eoghan</creator><creator>Mahony, Jennifer</creator><creator>O'Connell-Motherway, Mary</creator><creator>Bottacini, Francesca</creator><creator>Cornelissen, Anneleen</creator><creator>Neve, Horst</creator><creator>Heller, Knut J</creator><creator>Noben, Jean-Paul</creator><creator>Dal Bello, Fabio</creator><creator>van Sinderen, Douwe</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20140901</creationdate><title>Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages</title><author>Casey, Eoghan ; Mahony, Jennifer ; O'Connell-Motherway, Mary ; Bottacini, Francesca ; Cornelissen, Anneleen ; Neve, Horst ; Heller, Knut J ; Noben, Jean-Paul ; Dal Bello, Fabio ; van Sinderen, Douwe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-bc930e03b7d0e1d5ebf04df5608447c3df7b545047d7001d07877a2569a923b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Bacteriophages - chemistry</topic><topic>Bacteriophages - genetics</topic><topic>Bacteriophages - isolation & purification</topic><topic>Bacteriophages - physiology</topic><topic>DNA, Viral - genetics</topic><topic>Fermentation</topic><topic>Food Microbiology</topic><topic>Gene Expression Profiling</topic><topic>Gene Order</topic><topic>Genetics and Molecular Biology</topic><topic>Genomes</topic><topic>Gram-positive bacteria</topic><topic>Host Specificity</topic><topic>Lactobacillus delbrueckii</topic><topic>Lactobacillus delbrueckii - virology</topic><topic>Mass spectrometry</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular Sequence Data</topic><topic>Sequence Analysis, DNA</topic><topic>Synteny</topic><topic>Viral Proteins - analysis</topic><topic>Virion - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casey, Eoghan</creatorcontrib><creatorcontrib>Mahony, Jennifer</creatorcontrib><creatorcontrib>O'Connell-Motherway, Mary</creatorcontrib><creatorcontrib>Bottacini, Francesca</creatorcontrib><creatorcontrib>Cornelissen, Anneleen</creatorcontrib><creatorcontrib>Neve, Horst</creatorcontrib><creatorcontrib>Heller, Knut J</creatorcontrib><creatorcontrib>Noben, Jean-Paul</creatorcontrib><creatorcontrib>Dal Bello, Fabio</creatorcontrib><creatorcontrib>van Sinderen, Douwe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casey, Eoghan</au><au>Mahony, Jennifer</au><au>O'Connell-Motherway, Mary</au><au>Bottacini, Francesca</au><au>Cornelissen, Anneleen</au><au>Neve, Horst</au><au>Heller, Knut J</au><au>Noben, Jean-Paul</au><au>Dal Bello, Fabio</au><au>van Sinderen, Douwe</au><au>Björkroth, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>80</volume><issue>18</issue><spage>5623</spage><epage>5635</epage><pages>5623-5635</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>In this study, three phages infecting Lactobacillus delbrueckii subsp. bulgaricus, named Ld3, Ld17, and Ld25A, were isolated from whey samples obtained from various industrial fermentations. These phages were further characterized in a multifaceted approach: (i) biological and physical characterization through host range analysis and electron microscopy; (ii) genetic assessment through genome analysis; (iii) mass spectrometry analysis of the structural components of the phages; and (iv), for one phage, transcriptional analysis by Northern hybridization, reverse transcription-PCR, and primer extension. The three obtained phage genomes display high levels of sequence identity to each other and to genomes of the so-called group b L. delbrueckii phages c5, LL-Ku, and phiLdb, where some of the observed differences are believed to be responsible for host range variations.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>25002431</pmid><doi>10.1128/AEM.01268-14</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2014-09, Vol.80 (18), p.5623-5635 |
| issn | 0099-2240 1098-5336 1098-6596 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4178594 |
| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Bacteriophages - chemistry Bacteriophages - genetics Bacteriophages - isolation & purification Bacteriophages - physiology DNA, Viral - genetics Fermentation Food Microbiology Gene Expression Profiling Gene Order Genetics and Molecular Biology Genomes Gram-positive bacteria Host Specificity Lactobacillus delbrueckii Lactobacillus delbrueckii - virology Mass spectrometry Microscopy, Electron, Transmission Molecular Sequence Data Sequence Analysis, DNA Synteny Viral Proteins - analysis Virion - ultrastructure |
| title | Molecular characterization of three Lactobacillus delbrueckii subsp. bulgaricus phages |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T18%3A50%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20characterization%20of%20three%20Lactobacillus%20delbrueckii%20subsp.%20bulgaricus%20phages&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Casey,%20Eoghan&rft.date=2014-09-01&rft.volume=80&rft.issue=18&rft.spage=5623&rft.epage=5635&rft.pages=5623-5635&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.01268-14&rft_dat=%3Cproquest_pubme%3E3417500821%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1558315879&rft_id=info:pmid/25002431&rfr_iscdi=true |