Clinical significance of mucosal suppressors of cytokine signaling 3 expression in ulcerative colitis
To investigate the clinical significance of mucosal expression of suppressors of cytokine signaling 1 (SOCS1) and SOCS3 in human ulcerative colitis (UC). Biopsy specimens for histological analysis and mRNA detection were obtained endoscopically from the rectum of 62 patients with UC (36 men; age 13-...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2007-06, Vol.13 (21), p.2939-2944 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | Miyanaka, Yoshihiro Ueno, Yoshitaka Tanaka, Shinji Yoshioka, Kyoko Hatakeyama, Tsuyoshi Shimamoto, Masaru Sumii, Masaharu Chayama, Kazuaki |
| description | To investigate the clinical significance of mucosal expression of suppressors of cytokine signaling 1 (SOCS1) and SOCS3 in human ulcerative colitis (UC).
Biopsy specimens for histological analysis and mRNA detection were obtained endoscopically from the rectum of 62 patients with UC (36 men; age 13-76 years). The patients were classified endoscopically according to Matts' grade (grade 1 to 4). Expression of SOCS1 and SOCS3 mRNAs was quantified in samples by competitive reverse transcription-polymerase chain reaction (RT-PCR). GAPDH was used as an internal control for efficiency of RT-PCR and amount of RNA.
SOCS3 mRNA expression was significantly higher in inflamed mucosa of UC than in inactive mucosa. The level of expression was well correlated with the degree of both endoscopic and histologic inflammation. Interestingly, among the patients in remission, the group with relatively low expression of SOCS3 showed a higher rate of remission maintenance over a 12-mo period. In contrast, SOCS1 mRNA was expressed in both inflamed and non-inflamed colonic mucosa and was not correlated with the activity of colonic mucosa or prognosis.
These observations suggest that increased expression of mucosal SOCS3, but not of SOCS1, may play a critical role in the development of the colonic inflammation of UC. |
| doi_str_mv | 10.3748/wjg.v13.i21.2939 |
| format | Article |
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Biopsy specimens for histological analysis and mRNA detection were obtained endoscopically from the rectum of 62 patients with UC (36 men; age 13-76 years). The patients were classified endoscopically according to Matts' grade (grade 1 to 4). Expression of SOCS1 and SOCS3 mRNAs was quantified in samples by competitive reverse transcription-polymerase chain reaction (RT-PCR). GAPDH was used as an internal control for efficiency of RT-PCR and amount of RNA.
SOCS3 mRNA expression was significantly higher in inflamed mucosa of UC than in inactive mucosa. The level of expression was well correlated with the degree of both endoscopic and histologic inflammation. Interestingly, among the patients in remission, the group with relatively low expression of SOCS3 showed a higher rate of remission maintenance over a 12-mo period. In contrast, SOCS1 mRNA was expressed in both inflamed and non-inflamed colonic mucosa and was not correlated with the activity of colonic mucosa or prognosis.
These observations suggest that increased expression of mucosal SOCS3, but not of SOCS1, may play a critical role in the development of the colonic inflammation of UC.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v13.i21.2939</identifier><identifier>PMID: 17589943</identifier><language>eng</language><publisher>United States: Department of Medicine and Molecular Science, Hiroshima University,Hiroshima, Japan%Department of Endoscopy,Hiroshima University Hospital, Hiroshima, Japan%Department of Internal Medicine, Hiroshima Memorial Hospital, Japan</publisher><subject>Adolescent ; Adult ; Aged ; Clinical Research ; Colitis, Ulcerative - metabolism ; Colitis, Ulcerative - pathology ; Colon - metabolism ; Colon - pathology ; Female ; Gene Expression Regulation ; Humans ; Inflammation - metabolism ; Inflammation - pathology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Male ; Middle Aged ; Prognosis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Suppressor of Cytokine Signaling 1 Protein ; Suppressor of Cytokine Signaling 3 Protein ; Suppressor of Cytokine Signaling Proteins - genetics ; Suppressor of Cytokine Signaling Proteins - metabolism</subject><ispartof>World journal of gastroenterology : WJG, 2007-06, Vol.13 (21), p.2939-2944</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>2007 Baishideng Publishing Group Co., Limited. All rights reserved. 2007</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-4702bcb5a72dc5bb92df2ff3862b37f207a03b03c71e5664ed862c0662eee8bb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171145/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4171145/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17589943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyanaka, Yoshihiro</creatorcontrib><creatorcontrib>Ueno, Yoshitaka</creatorcontrib><creatorcontrib>Tanaka, Shinji</creatorcontrib><creatorcontrib>Yoshioka, Kyoko</creatorcontrib><creatorcontrib>Hatakeyama, Tsuyoshi</creatorcontrib><creatorcontrib>Shimamoto, Masaru</creatorcontrib><creatorcontrib>Sumii, Masaharu</creatorcontrib><creatorcontrib>Chayama, Kazuaki</creatorcontrib><title>Clinical significance of mucosal suppressors of cytokine signaling 3 expression in ulcerative colitis</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To investigate the clinical significance of mucosal expression of suppressors of cytokine signaling 1 (SOCS1) and SOCS3 in human ulcerative colitis (UC).
Biopsy specimens for histological analysis and mRNA detection were obtained endoscopically from the rectum of 62 patients with UC (36 men; age 13-76 years). The patients were classified endoscopically according to Matts' grade (grade 1 to 4). Expression of SOCS1 and SOCS3 mRNAs was quantified in samples by competitive reverse transcription-polymerase chain reaction (RT-PCR). GAPDH was used as an internal control for efficiency of RT-PCR and amount of RNA.
SOCS3 mRNA expression was significantly higher in inflamed mucosa of UC than in inactive mucosa. The level of expression was well correlated with the degree of both endoscopic and histologic inflammation. Interestingly, among the patients in remission, the group with relatively low expression of SOCS3 showed a higher rate of remission maintenance over a 12-mo period. In contrast, SOCS1 mRNA was expressed in both inflamed and non-inflamed colonic mucosa and was not correlated with the activity of colonic mucosa or prognosis.
These observations suggest that increased expression of mucosal SOCS3, but not of SOCS1, may play a critical role in the development of the colonic inflammation of UC.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Clinical Research</subject><subject>Colitis, Ulcerative - metabolism</subject><subject>Colitis, Ulcerative - pathology</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - pathology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Prognosis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Suppressor of Cytokine Signaling 1 Protein</subject><subject>Suppressor of Cytokine Signaling 3 Protein</subject><subject>Suppressor of Cytokine Signaling Proteins - genetics</subject><subject>Suppressor of Cytokine Signaling Proteins - metabolism</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUcFO3DAQtaqisoXee0I5VNyytcdJnFyQqhW0lZC4wNmyvePU26y92MlS_r4Ou6LlZGvmvTcz7xHymdElF1X79WnTL_eMLx2wJXS8e0cWAKwroa3oe7JglIqy4yBOyceUNpQC5zV8IKdM1G3XVXxBcDU474waiuR672z-eoNFsMV2MiHN9Wm3i5hSiGkum-cx_HYeX_Aqk_uCF_jnBeKCL5wvpsFgVKPbY2HC4EaXzsmJVUPCT8f3jDzcXN-vfpS3d99_rr7dlqYCPpaVoKCNrpWAtam17mBtwVreNqC5sECFolxTbgTDumkqXOeOoU0DiNhqzc_I1UF3N-ktrg36MapB7qLbqvgsg3Lybce7X7IPe1kxwVhVZ4EvB4En5a3yvdyEKeYzk8xWQ7YTsqdNhl0e58TwOGEa5dYlg8OgPIYpSTHvVAuWgfQANDGkFNG-7sKonCOcdWWOUOYI5Rxhplz8f8M_wjEz_hcrR5vC</recordid><startdate>20070607</startdate><enddate>20070607</enddate><creator>Miyanaka, Yoshihiro</creator><creator>Ueno, Yoshitaka</creator><creator>Tanaka, Shinji</creator><creator>Yoshioka, Kyoko</creator><creator>Hatakeyama, Tsuyoshi</creator><creator>Shimamoto, Masaru</creator><creator>Sumii, Masaharu</creator><creator>Chayama, Kazuaki</creator><general>Department of Medicine and Molecular Science, Hiroshima University,Hiroshima, Japan%Department of Endoscopy,Hiroshima University Hospital, Hiroshima, Japan%Department of Internal Medicine, Hiroshima Memorial Hospital, Japan</general><general>Baishideng Publishing Group Co., Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20070607</creationdate><title>Clinical significance of mucosal suppressors of cytokine signaling 3 expression in ulcerative colitis</title><author>Miyanaka, Yoshihiro ; Ueno, Yoshitaka ; Tanaka, Shinji ; Yoshioka, Kyoko ; Hatakeyama, Tsuyoshi ; Shimamoto, Masaru ; Sumii, Masaharu ; Chayama, Kazuaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-4702bcb5a72dc5bb92df2ff3862b37f207a03b03c71e5664ed862c0662eee8bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Clinical Research</topic><topic>Colitis, Ulcerative - metabolism</topic><topic>Colitis, Ulcerative - pathology</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - pathology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Prognosis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Suppressor of Cytokine Signaling 1 Protein</topic><topic>Suppressor of Cytokine Signaling 3 Protein</topic><topic>Suppressor of Cytokine Signaling Proteins - genetics</topic><topic>Suppressor of Cytokine Signaling Proteins - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Miyanaka, Yoshihiro</creatorcontrib><creatorcontrib>Ueno, Yoshitaka</creatorcontrib><creatorcontrib>Tanaka, Shinji</creatorcontrib><creatorcontrib>Yoshioka, Kyoko</creatorcontrib><creatorcontrib>Hatakeyama, Tsuyoshi</creatorcontrib><creatorcontrib>Shimamoto, Masaru</creatorcontrib><creatorcontrib>Sumii, Masaharu</creatorcontrib><creatorcontrib>Chayama, Kazuaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miyanaka, Yoshihiro</au><au>Ueno, Yoshitaka</au><au>Tanaka, Shinji</au><au>Yoshioka, Kyoko</au><au>Hatakeyama, Tsuyoshi</au><au>Shimamoto, Masaru</au><au>Sumii, Masaharu</au><au>Chayama, Kazuaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical significance of mucosal suppressors of cytokine signaling 3 expression in ulcerative colitis</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2007-06-07</date><risdate>2007</risdate><volume>13</volume><issue>21</issue><spage>2939</spage><epage>2944</epage><pages>2939-2944</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To investigate the clinical significance of mucosal expression of suppressors of cytokine signaling 1 (SOCS1) and SOCS3 in human ulcerative colitis (UC).
Biopsy specimens for histological analysis and mRNA detection were obtained endoscopically from the rectum of 62 patients with UC (36 men; age 13-76 years). The patients were classified endoscopically according to Matts' grade (grade 1 to 4). Expression of SOCS1 and SOCS3 mRNAs was quantified in samples by competitive reverse transcription-polymerase chain reaction (RT-PCR). GAPDH was used as an internal control for efficiency of RT-PCR and amount of RNA.
SOCS3 mRNA expression was significantly higher in inflamed mucosa of UC than in inactive mucosa. The level of expression was well correlated with the degree of both endoscopic and histologic inflammation. Interestingly, among the patients in remission, the group with relatively low expression of SOCS3 showed a higher rate of remission maintenance over a 12-mo period. In contrast, SOCS1 mRNA was expressed in both inflamed and non-inflamed colonic mucosa and was not correlated with the activity of colonic mucosa or prognosis.
These observations suggest that increased expression of mucosal SOCS3, but not of SOCS1, may play a critical role in the development of the colonic inflammation of UC.</abstract><cop>United States</cop><pub>Department of Medicine and Molecular Science, Hiroshima University,Hiroshima, Japan%Department of Endoscopy,Hiroshima University Hospital, Hiroshima, Japan%Department of Internal Medicine, Hiroshima Memorial Hospital, Japan</pub><pmid>17589943</pmid><doi>10.3748/wjg.v13.i21.2939</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Aged Clinical Research Colitis, Ulcerative - metabolism Colitis, Ulcerative - pathology Colon - metabolism Colon - pathology Female Gene Expression Regulation Humans Inflammation - metabolism Inflammation - pathology Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Male Middle Aged Prognosis RNA, Messenger - genetics RNA, Messenger - metabolism Suppressor of Cytokine Signaling 1 Protein Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins - genetics Suppressor of Cytokine Signaling Proteins - metabolism |
| title | Clinical significance of mucosal suppressors of cytokine signaling 3 expression in ulcerative colitis |
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