Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators
Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with...
Gespeichert in:
| Veröffentlicht in: | Microbiome 2014-08, Vol.2 (1), p.32-32, Article 32 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 32 |
|---|---|
| container_issue | 1 |
| container_start_page | 32 |
| container_title | Microbiome |
| container_volume | 2 |
| creator | Galimanas, Vaia Hall, Michael William Singh, Natasha Lynch, Michael David Joseph Goldberg, Michael Tenenbaum, Howard Cvitkovitch, Dennis Gerard Neufeld, Josh David Senadheera, Dilani Braziunas |
| description | Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with the disease. Until now, microbial plaque obtained from the subgingival (SubG) sites has been the primary focus for bacterial community analysis using deep sequencing. In addition to the use of SubG plaque, here, we investigated whether plaque obtained from supragingival (SupG) and tongue dorsum sites can serve as alternatives for monitoring CP-associated bacterial biomarkers.
Using SubG, SupG, and tongue plaque DNA from 11 healthy and 13 diseased subjects, we sequenced V3 regions (approximately 200 bases) of the 16S rRNA gene using Illumina sequencing. After quality filtering, approximately 4.1 million sequences were collapsed into operational taxonomic units (OTUs; sequence identity cutoff of >97%) that were classified to a total of 19 phyla spanning 114 genera. Bacterial community diversity and overall composition was not affected by health or disease, and multiresponse permutation procedure (MRPP) on Bray-Curtis distance measures only supported weakly distinct bacterial communities in SubG and tongue plaque depending on health or disease status (P |
| doi_str_mv | 10.1186/2049-2618-2-32 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4164120</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A541258549</galeid><sourcerecordid>A541258549</sourcerecordid><originalsourceid>FETCH-LOGICAL-c491t-2eaa81d99bec618cb5496f201db74212aa1e34643c7fb0b9980491140c3740d3</originalsourceid><addsrcrecordid>eNptkk1r3DAQhkVpaUKaa49F0EtzcKqRZa19KaShH4FAIcldyNJ4o2JLriSnyb-vTNIlC5EOGmae92VGDCHvgZ0CtPIzZ6KruIS24lXNX5HDXeL1s_iAHKf0m5XTgdiI9i054A3njQR2SP5-1SZjdHqkJkzT4l1-WKM5JJdd8DQM1NzG4J2hc-GCDT6XSqLaW-rDHY40xKJOeppH57e06DDRIURqMaPJa866hDohdd46o3OI6R15M-gx4fHTe0Ruvn-7Of9ZXf76cXF-dlkZ0UGuOGrdgu26Hk0ZxfSN6OTAGdh-IzhwrQFrIUVtNkPP-q5ry9QAgpl6I5itj8iXR9t56Se0Bn0uzao5uknHBxW0U_sV727VNtwpAVIAZ8Xg05NBDH8WTFlNLhkcR-0xLElBI7mUHCQU9OMjutUjKueHUBzNiquzppg1bWm-UKcvUOVanJwJHgdX8nuCkz1BYTLe561eUlIX11cvmpsYUoo47CYFptaVUetWqHUrFFc1L4IPz_9nh_9fkPofIpK8aA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1562662161</pqid></control><display><type>article</type><title>Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators</title><source>DOAJ Directory of Open Access Journals</source><source>SpringerLink Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><creator>Galimanas, Vaia ; Hall, Michael William ; Singh, Natasha ; Lynch, Michael David Joseph ; Goldberg, Michael ; Tenenbaum, Howard ; Cvitkovitch, Dennis Gerard ; Neufeld, Josh David ; Senadheera, Dilani Braziunas</creator><creatorcontrib>Galimanas, Vaia ; Hall, Michael William ; Singh, Natasha ; Lynch, Michael David Joseph ; Goldberg, Michael ; Tenenbaum, Howard ; Cvitkovitch, Dennis Gerard ; Neufeld, Josh David ; Senadheera, Dilani Braziunas</creatorcontrib><description>Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with the disease. Until now, microbial plaque obtained from the subgingival (SubG) sites has been the primary focus for bacterial community analysis using deep sequencing. In addition to the use of SubG plaque, here, we investigated whether plaque obtained from supragingival (SupG) and tongue dorsum sites can serve as alternatives for monitoring CP-associated bacterial biomarkers.
Using SubG, SupG, and tongue plaque DNA from 11 healthy and 13 diseased subjects, we sequenced V3 regions (approximately 200 bases) of the 16S rRNA gene using Illumina sequencing. After quality filtering, approximately 4.1 million sequences were collapsed into operational taxonomic units (OTUs; sequence identity cutoff of >97%) that were classified to a total of 19 phyla spanning 114 genera. Bacterial community diversity and overall composition was not affected by health or disease, and multiresponse permutation procedure (MRPP) on Bray-Curtis distance measures only supported weakly distinct bacterial communities in SubG and tongue plaque depending on health or disease status (P < 0.05). Nonetheless, in SubG and tongue sites, the relative abundance of Firmicutes was increased significantly from health to disease and members of Synergistetes were found in higher abundance across all sites in disease. Taxa indicative of CP were identified in all three locations (for example, Treponema denticola, Porphyromonas gingivalis, Synergistes oral taxa 362 and 363).
For the first time, this study demonstrates that SupG and tongue dorsum plaque can serve as alternative sources for detecting and enumerating known and novel bacterial biomarkers of CP. This finding is clinically important because, in contrast with SubG sampling that requires trained professionals, obtaining plaque from SupG and tongue sites is convenient and minimally-invasive and offers a novel means to track CP-biomarker organisms during treatment outcome monitoring.</description><identifier>ISSN: 2049-2618</identifier><identifier>EISSN: 2049-2618</identifier><identifier>DOI: 10.1186/2049-2618-2-32</identifier><identifier>PMID: 25225610</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Consortia ; Development and progression ; DNA sequencing ; Nucleotide sequencing ; Periodontitis ; Physiological aspects</subject><ispartof>Microbiome, 2014-08, Vol.2 (1), p.32-32, Article 32</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>Copyright © 2014 Galimanas et al.; licensee BioMed Central Ltd. 2014 Galimanas et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-2eaa81d99bec618cb5496f201db74212aa1e34643c7fb0b9980491140c3740d3</citedby><cites>FETCH-LOGICAL-c491t-2eaa81d99bec618cb5496f201db74212aa1e34643c7fb0b9980491140c3740d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164120/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4164120/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25225610$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Galimanas, Vaia</creatorcontrib><creatorcontrib>Hall, Michael William</creatorcontrib><creatorcontrib>Singh, Natasha</creatorcontrib><creatorcontrib>Lynch, Michael David Joseph</creatorcontrib><creatorcontrib>Goldberg, Michael</creatorcontrib><creatorcontrib>Tenenbaum, Howard</creatorcontrib><creatorcontrib>Cvitkovitch, Dennis Gerard</creatorcontrib><creatorcontrib>Neufeld, Josh David</creatorcontrib><creatorcontrib>Senadheera, Dilani Braziunas</creatorcontrib><title>Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators</title><title>Microbiome</title><addtitle>Microbiome</addtitle><description>Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with the disease. Until now, microbial plaque obtained from the subgingival (SubG) sites has been the primary focus for bacterial community analysis using deep sequencing. In addition to the use of SubG plaque, here, we investigated whether plaque obtained from supragingival (SupG) and tongue dorsum sites can serve as alternatives for monitoring CP-associated bacterial biomarkers.
Using SubG, SupG, and tongue plaque DNA from 11 healthy and 13 diseased subjects, we sequenced V3 regions (approximately 200 bases) of the 16S rRNA gene using Illumina sequencing. After quality filtering, approximately 4.1 million sequences were collapsed into operational taxonomic units (OTUs; sequence identity cutoff of >97%) that were classified to a total of 19 phyla spanning 114 genera. Bacterial community diversity and overall composition was not affected by health or disease, and multiresponse permutation procedure (MRPP) on Bray-Curtis distance measures only supported weakly distinct bacterial communities in SubG and tongue plaque depending on health or disease status (P < 0.05). Nonetheless, in SubG and tongue sites, the relative abundance of Firmicutes was increased significantly from health to disease and members of Synergistetes were found in higher abundance across all sites in disease. Taxa indicative of CP were identified in all three locations (for example, Treponema denticola, Porphyromonas gingivalis, Synergistes oral taxa 362 and 363).
For the first time, this study demonstrates that SupG and tongue dorsum plaque can serve as alternative sources for detecting and enumerating known and novel bacterial biomarkers of CP. This finding is clinically important because, in contrast with SubG sampling that requires trained professionals, obtaining plaque from SupG and tongue sites is convenient and minimally-invasive and offers a novel means to track CP-biomarker organisms during treatment outcome monitoring.</description><subject>Analysis</subject><subject>Consortia</subject><subject>Development and progression</subject><subject>DNA sequencing</subject><subject>Nucleotide sequencing</subject><subject>Periodontitis</subject><subject>Physiological aspects</subject><issn>2049-2618</issn><issn>2049-2618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNptkk1r3DAQhkVpaUKaa49F0EtzcKqRZa19KaShH4FAIcldyNJ4o2JLriSnyb-vTNIlC5EOGmae92VGDCHvgZ0CtPIzZ6KruIS24lXNX5HDXeL1s_iAHKf0m5XTgdiI9i054A3njQR2SP5-1SZjdHqkJkzT4l1-WKM5JJdd8DQM1NzG4J2hc-GCDT6XSqLaW-rDHY40xKJOeppH57e06DDRIURqMaPJa866hDohdd46o3OI6R15M-gx4fHTe0Ruvn-7Of9ZXf76cXF-dlkZ0UGuOGrdgu26Hk0ZxfSN6OTAGdh-IzhwrQFrIUVtNkPP-q5ry9QAgpl6I5itj8iXR9t56Se0Bn0uzao5uknHBxW0U_sV727VNtwpAVIAZ8Xg05NBDH8WTFlNLhkcR-0xLElBI7mUHCQU9OMjutUjKueHUBzNiquzppg1bWm-UKcvUOVanJwJHgdX8nuCkz1BYTLe561eUlIX11cvmpsYUoo47CYFptaVUetWqHUrFFc1L4IPz_9nh_9fkPofIpK8aA</recordid><startdate>20140826</startdate><enddate>20140826</enddate><creator>Galimanas, Vaia</creator><creator>Hall, Michael William</creator><creator>Singh, Natasha</creator><creator>Lynch, Michael David Joseph</creator><creator>Goldberg, Michael</creator><creator>Tenenbaum, Howard</creator><creator>Cvitkovitch, Dennis Gerard</creator><creator>Neufeld, Josh David</creator><creator>Senadheera, Dilani Braziunas</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140826</creationdate><title>Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators</title><author>Galimanas, Vaia ; Hall, Michael William ; Singh, Natasha ; Lynch, Michael David Joseph ; Goldberg, Michael ; Tenenbaum, Howard ; Cvitkovitch, Dennis Gerard ; Neufeld, Josh David ; Senadheera, Dilani Braziunas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-2eaa81d99bec618cb5496f201db74212aa1e34643c7fb0b9980491140c3740d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Analysis</topic><topic>Consortia</topic><topic>Development and progression</topic><topic>DNA sequencing</topic><topic>Nucleotide sequencing</topic><topic>Periodontitis</topic><topic>Physiological aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galimanas, Vaia</creatorcontrib><creatorcontrib>Hall, Michael William</creatorcontrib><creatorcontrib>Singh, Natasha</creatorcontrib><creatorcontrib>Lynch, Michael David Joseph</creatorcontrib><creatorcontrib>Goldberg, Michael</creatorcontrib><creatorcontrib>Tenenbaum, Howard</creatorcontrib><creatorcontrib>Cvitkovitch, Dennis Gerard</creatorcontrib><creatorcontrib>Neufeld, Josh David</creatorcontrib><creatorcontrib>Senadheera, Dilani Braziunas</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Microbiome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galimanas, Vaia</au><au>Hall, Michael William</au><au>Singh, Natasha</au><au>Lynch, Michael David Joseph</au><au>Goldberg, Michael</au><au>Tenenbaum, Howard</au><au>Cvitkovitch, Dennis Gerard</au><au>Neufeld, Josh David</au><au>Senadheera, Dilani Braziunas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators</atitle><jtitle>Microbiome</jtitle><addtitle>Microbiome</addtitle><date>2014-08-26</date><risdate>2014</risdate><volume>2</volume><issue>1</issue><spage>32</spage><epage>32</epage><pages>32-32</pages><artnum>32</artnum><issn>2049-2618</issn><eissn>2049-2618</eissn><abstract>Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with the disease. Until now, microbial plaque obtained from the subgingival (SubG) sites has been the primary focus for bacterial community analysis using deep sequencing. In addition to the use of SubG plaque, here, we investigated whether plaque obtained from supragingival (SupG) and tongue dorsum sites can serve as alternatives for monitoring CP-associated bacterial biomarkers.
Using SubG, SupG, and tongue plaque DNA from 11 healthy and 13 diseased subjects, we sequenced V3 regions (approximately 200 bases) of the 16S rRNA gene using Illumina sequencing. After quality filtering, approximately 4.1 million sequences were collapsed into operational taxonomic units (OTUs; sequence identity cutoff of >97%) that were classified to a total of 19 phyla spanning 114 genera. Bacterial community diversity and overall composition was not affected by health or disease, and multiresponse permutation procedure (MRPP) on Bray-Curtis distance measures only supported weakly distinct bacterial communities in SubG and tongue plaque depending on health or disease status (P < 0.05). Nonetheless, in SubG and tongue sites, the relative abundance of Firmicutes was increased significantly from health to disease and members of Synergistetes were found in higher abundance across all sites in disease. Taxa indicative of CP were identified in all three locations (for example, Treponema denticola, Porphyromonas gingivalis, Synergistes oral taxa 362 and 363).
For the first time, this study demonstrates that SupG and tongue dorsum plaque can serve as alternative sources for detecting and enumerating known and novel bacterial biomarkers of CP. This finding is clinically important because, in contrast with SubG sampling that requires trained professionals, obtaining plaque from SupG and tongue sites is convenient and minimally-invasive and offers a novel means to track CP-biomarker organisms during treatment outcome monitoring.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>25225610</pmid><doi>10.1186/2049-2618-2-32</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2049-2618 |
| ispartof | Microbiome, 2014-08, Vol.2 (1), p.32-32, Article 32 |
| issn | 2049-2618 2049-2618 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4164120 |
| source | DOAJ Directory of Open Access Journals; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Analysis Consortia Development and progression DNA sequencing Nucleotide sequencing Periodontitis Physiological aspects |
| title | Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T00%3A24%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20community%20composition%20of%20chronic%20periodontitis%20and%20novel%20oral%20sampling%20sites%20for%20detecting%20disease%20indicators&rft.jtitle=Microbiome&rft.au=Galimanas,%20Vaia&rft.date=2014-08-26&rft.volume=2&rft.issue=1&rft.spage=32&rft.epage=32&rft.pages=32-32&rft.artnum=32&rft.issn=2049-2618&rft.eissn=2049-2618&rft_id=info:doi/10.1186/2049-2618-2-32&rft_dat=%3Cgale_pubme%3EA541258549%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1562662161&rft_id=info:pmid/25225610&rft_galeid=A541258549&rfr_iscdi=true |