Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes

Flavin‐dependent oxidoreductases are increasingly recognized as important biocatalysts for various industrial applications. In order to identify novel activities and to improve these enzymes in engineering approaches, suitable screening methods are necessary. We developed novel microtiter‐plate‐base...

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Veröffentlicht in:	Biotechnology journal 2014-04, Vol.9 (4), p.474-482
Hauptverfasser:	Brugger, Dagmar, Krondorfer, Iris, Zahma, Kawah, Stoisser, Thomas, Bolivar, Juan M., Nidetzky, Bernd, Peterbauer, Clemens K., Haltrich, Dietmar
Format:	Artikel
Sprache:	eng
Schlagworte:	2,6-Dichloroindophenol - analysis 2,6-Dichloroindophenol - chemistry 2,6-Dichloroindophenol - metabolism 2,6‐dichlorophenol‐indophenol 6-dichlorophenol-indophenol Coloring Agents - analysis Coloring Agents - chemistry Coloring Agents - metabolism Enzyme Assays - methods flavin-dependent oxidoreductases high-throughput screening High-Throughput Screening Assays Methylene Blue - analogs & derivatives Methylene Blue - analysis Methylene Blue - chemistry Methylene Blue - metabolism methylene green Oxidation-Reduction Oxidoreductases - metabolism Phenothiazines - analysis Phenothiazines - chemistry Phenothiazines - metabolism thionine
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