GPC3 reduces cell proliferation in renal carcinoma cell lines
Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth...
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| Veröffentlicht in: | BMC cancer 2014-08, Vol.14 (1), p.631-631, Article 631 |
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| creator | Valsechi, Marina Curado Oliveira, Ana Beatriz Bortolozo Conceição, André Luis Giacometti Stuqui, Bruna Candido, Natalia Maria Provazzi, Paola Jocelan Scarin de Araújo, Luiza Ferreira Silva, Jr, Wilson Araújo Calmon, Marilia de Freitas Rahal, Paula |
| description | Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma.
Five clear cell renal cell carcinoma cell lines and carcinoma samples were used to analyze GPC3 mRNA expression (qRT-PCR). Then, representative cell lines, one primary renal carcinoma (786-O) and one metastatic renal carcinoma (ACHN), were chosen to carry out functional studies. We constructed a GPC3 expression vector and transfected the renal carcinoma cell lines, 786-O and ACHN. GPC3 overexpression was analyzed using qRT-PCR and immunocytochemistry. We evaluated cell proliferation using MTT and colony formation assays. Flow cytometry was used to evaluate apoptosis and perform cell cycle analyses.
We observed that GPC3 is downregulated in clear cell renal cell carcinoma samples and cell lines compared with normal renal samples. GPC3 mRNA expression and protein levels in 786-O and ACHN cell lines increased after transfection with the GPC3 expression construct, and the cell proliferation rate decreased in both cell lines following overexpression of GPC3. Further, apoptosis was not induced in the renal cell carcinoma cell lines overexpressing GPC3, and there was an increase in the cell population during the G1 phase in the cell cycle.
We suggest that the GPC3 gene reduces the rate of cell proliferation through cell cycle arrest during the G1 phase in renal cell carcinoma. |
| doi_str_mv | 10.1186/1471-2407-14-631 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4161903</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1561467177</sourcerecordid><originalsourceid>FETCH-LOGICAL-b484t-9444ede247ff393494c755555ae5b09b3ef9b408b352f4b949758c03add8ea643</originalsourceid><addsrcrecordid>eNp1kc1LxDAQxYMoun7cPUnBi5dqppkm7UFBFr9gQQ96Dmmaapa2WZOt4H9vS9dlFc0lw8zL4-U3hBwDPQfI-AWggDhBKmLAmDPYIpN1a3uj3iP7IcwpBZHRbJfsJSnwDDifkMu7pymLvCk7bUKkTV1HC-9qWxmvlta1kW37aavqSCuvbesaNapq25pwSHYqVQdztLoPyMvtzfP0Pp493j1Mr2dxgRku4xwRTWkSFFXFcoY5apEOR5m0oHnBTJUXSLOCpUmFRY65SDNNmSrLzCiO7IBcjb6LrmhMqU279KqWC28b5T-lU1b-nLT2Tb66D4nAIaesN5iOBoV1_xj8nGjXyAGfHPD1lezp9i5nqxjevXcmLGVjw0BDtcZ1QULKAbkAIXrp6S_p3HW-5ziqIIU0Gf5FR5X2LgRvqnUioHLY8F8ZTjZRrB98r5R9AWosoDY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1561151524</pqid></control><display><type>article</type><title>GPC3 reduces cell proliferation in renal carcinoma cell lines</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Springer Nature OA Free Journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Valsechi, Marina Curado ; Oliveira, Ana Beatriz Bortolozo ; Conceição, André Luis Giacometti ; Stuqui, Bruna ; Candido, Natalia Maria ; Provazzi, Paola Jocelan Scarin ; de Araújo, Luiza Ferreira ; Silva, Jr, Wilson Araújo ; Calmon, Marilia de Freitas ; Rahal, Paula</creator><creatorcontrib>Valsechi, Marina Curado ; Oliveira, Ana Beatriz Bortolozo ; Conceição, André Luis Giacometti ; Stuqui, Bruna ; Candido, Natalia Maria ; Provazzi, Paola Jocelan Scarin ; de Araújo, Luiza Ferreira ; Silva, Jr, Wilson Araújo ; Calmon, Marilia de Freitas ; Rahal, Paula</creatorcontrib><description>Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma.
Five clear cell renal cell carcinoma cell lines and carcinoma samples were used to analyze GPC3 mRNA expression (qRT-PCR). Then, representative cell lines, one primary renal carcinoma (786-O) and one metastatic renal carcinoma (ACHN), were chosen to carry out functional studies. We constructed a GPC3 expression vector and transfected the renal carcinoma cell lines, 786-O and ACHN. GPC3 overexpression was analyzed using qRT-PCR and immunocytochemistry. We evaluated cell proliferation using MTT and colony formation assays. Flow cytometry was used to evaluate apoptosis and perform cell cycle analyses.
We observed that GPC3 is downregulated in clear cell renal cell carcinoma samples and cell lines compared with normal renal samples. GPC3 mRNA expression and protein levels in 786-O and ACHN cell lines increased after transfection with the GPC3 expression construct, and the cell proliferation rate decreased in both cell lines following overexpression of GPC3. Further, apoptosis was not induced in the renal cell carcinoma cell lines overexpressing GPC3, and there was an increase in the cell population during the G1 phase in the cell cycle.
We suggest that the GPC3 gene reduces the rate of cell proliferation through cell cycle arrest during the G1 phase in renal cell carcinoma.</description><identifier>ISSN: 1471-2407</identifier><identifier>EISSN: 1471-2407</identifier><identifier>DOI: 10.1186/1471-2407-14-631</identifier><identifier>PMID: 25168166</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Apoptosis ; Breast cancer ; Carcinoma, Renal Cell - genetics ; Carcinoma, Renal Cell - metabolism ; Carcinoma, Renal Cell - pathology ; Cell Cycle ; Cell growth ; Cell Line, Tumor ; Cell Proliferation ; Deoxyribonucleic acid ; DNA ; Extracellular matrix ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Genomics ; Glypicans - genetics ; Glypicans - metabolism ; Humans ; Kidney Neoplasms - genetics ; Kidney Neoplasms - metabolism ; Kidney Neoplasms - pathology ; Liver cancer ; Male ; Metastasis ; Middle Aged ; Polymerase chain reaction ; Real time ; Rodents ; Studies ; Tumors</subject><ispartof>BMC cancer, 2014-08, Vol.14 (1), p.631-631, Article 631</ispartof><rights>2014 Valsechi et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>Valsechi et al.; licensee BioMed Central Ltd. 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b484t-9444ede247ff393494c755555ae5b09b3ef9b408b352f4b949758c03add8ea643</citedby><cites>FETCH-LOGICAL-b484t-9444ede247ff393494c755555ae5b09b3ef9b408b352f4b949758c03add8ea643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161903/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4161903/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,862,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25168166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valsechi, Marina Curado</creatorcontrib><creatorcontrib>Oliveira, Ana Beatriz Bortolozo</creatorcontrib><creatorcontrib>Conceição, André Luis Giacometti</creatorcontrib><creatorcontrib>Stuqui, Bruna</creatorcontrib><creatorcontrib>Candido, Natalia Maria</creatorcontrib><creatorcontrib>Provazzi, Paola Jocelan Scarin</creatorcontrib><creatorcontrib>de Araújo, Luiza Ferreira</creatorcontrib><creatorcontrib>Silva, Jr, Wilson Araújo</creatorcontrib><creatorcontrib>Calmon, Marilia de Freitas</creatorcontrib><creatorcontrib>Rahal, Paula</creatorcontrib><title>GPC3 reduces cell proliferation in renal carcinoma cell lines</title><title>BMC cancer</title><addtitle>BMC Cancer</addtitle><description>Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma.
Five clear cell renal cell carcinoma cell lines and carcinoma samples were used to analyze GPC3 mRNA expression (qRT-PCR). Then, representative cell lines, one primary renal carcinoma (786-O) and one metastatic renal carcinoma (ACHN), were chosen to carry out functional studies. We constructed a GPC3 expression vector and transfected the renal carcinoma cell lines, 786-O and ACHN. GPC3 overexpression was analyzed using qRT-PCR and immunocytochemistry. We evaluated cell proliferation using MTT and colony formation assays. Flow cytometry was used to evaluate apoptosis and perform cell cycle analyses.
We observed that GPC3 is downregulated in clear cell renal cell carcinoma samples and cell lines compared with normal renal samples. GPC3 mRNA expression and protein levels in 786-O and ACHN cell lines increased after transfection with the GPC3 expression construct, and the cell proliferation rate decreased in both cell lines following overexpression of GPC3. Further, apoptosis was not induced in the renal cell carcinoma cell lines overexpressing GPC3, and there was an increase in the cell population during the G1 phase in the cell cycle.
We suggest that the GPC3 gene reduces the rate of cell proliferation through cell cycle arrest during the G1 phase in renal cell carcinoma.</description><subject>Apoptosis</subject><subject>Breast cancer</subject><subject>Carcinoma, Renal Cell - genetics</subject><subject>Carcinoma, Renal Cell - metabolism</subject><subject>Carcinoma, Renal Cell - pathology</subject><subject>Cell Cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Extracellular matrix</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genomics</subject><subject>Glypicans - genetics</subject><subject>Glypicans - metabolism</subject><subject>Humans</subject><subject>Kidney Neoplasms - genetics</subject><subject>Kidney Neoplasms - metabolism</subject><subject>Kidney Neoplasms - pathology</subject><subject>Liver cancer</subject><subject>Male</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Polymerase chain reaction</subject><subject>Real time</subject><subject>Rodents</subject><subject>Studies</subject><subject>Tumors</subject><issn>1471-2407</issn><issn>1471-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kc1LxDAQxYMoun7cPUnBi5dqppkm7UFBFr9gQQ96Dmmaapa2WZOt4H9vS9dlFc0lw8zL4-U3hBwDPQfI-AWggDhBKmLAmDPYIpN1a3uj3iP7IcwpBZHRbJfsJSnwDDifkMu7pymLvCk7bUKkTV1HC-9qWxmvlta1kW37aavqSCuvbesaNapq25pwSHYqVQdztLoPyMvtzfP0Pp493j1Mr2dxgRku4xwRTWkSFFXFcoY5apEOR5m0oHnBTJUXSLOCpUmFRY65SDNNmSrLzCiO7IBcjb6LrmhMqU279KqWC28b5T-lU1b-nLT2Tb66D4nAIaesN5iOBoV1_xj8nGjXyAGfHPD1lezp9i5nqxjevXcmLGVjw0BDtcZ1QULKAbkAIXrp6S_p3HW-5ziqIIU0Gf5FR5X2LgRvqnUioHLY8F8ZTjZRrB98r5R9AWosoDY</recordid><startdate>20140829</startdate><enddate>20140829</enddate><creator>Valsechi, Marina Curado</creator><creator>Oliveira, Ana Beatriz Bortolozo</creator><creator>Conceição, André Luis Giacometti</creator><creator>Stuqui, Bruna</creator><creator>Candido, Natalia Maria</creator><creator>Provazzi, Paola Jocelan Scarin</creator><creator>de Araújo, Luiza Ferreira</creator><creator>Silva, Jr, Wilson Araújo</creator><creator>Calmon, Marilia de Freitas</creator><creator>Rahal, Paula</creator><general>BioMed Central</general><general>BioMed Central Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140829</creationdate><title>GPC3 reduces cell proliferation in renal carcinoma cell lines</title><author>Valsechi, Marina Curado ; Oliveira, Ana Beatriz Bortolozo ; Conceição, André Luis Giacometti ; Stuqui, Bruna ; Candido, Natalia Maria ; Provazzi, Paola Jocelan Scarin ; de Araújo, Luiza Ferreira ; Silva, Jr, Wilson Araújo ; Calmon, Marilia de Freitas ; Rahal, Paula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b484t-9444ede247ff393494c755555ae5b09b3ef9b408b352f4b949758c03add8ea643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis</topic><topic>Breast cancer</topic><topic>Carcinoma, Renal Cell - genetics</topic><topic>Carcinoma, Renal Cell - metabolism</topic><topic>Carcinoma, Renal Cell - pathology</topic><topic>Cell Cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Extracellular matrix</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genomics</topic><topic>Glypicans - genetics</topic><topic>Glypicans - metabolism</topic><topic>Humans</topic><topic>Kidney Neoplasms - genetics</topic><topic>Kidney Neoplasms - metabolism</topic><topic>Kidney Neoplasms - pathology</topic><topic>Liver cancer</topic><topic>Male</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Polymerase chain reaction</topic><topic>Real time</topic><topic>Rodents</topic><topic>Studies</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valsechi, Marina Curado</creatorcontrib><creatorcontrib>Oliveira, Ana Beatriz Bortolozo</creatorcontrib><creatorcontrib>Conceição, André Luis Giacometti</creatorcontrib><creatorcontrib>Stuqui, Bruna</creatorcontrib><creatorcontrib>Candido, Natalia Maria</creatorcontrib><creatorcontrib>Provazzi, Paola Jocelan Scarin</creatorcontrib><creatorcontrib>de Araújo, Luiza Ferreira</creatorcontrib><creatorcontrib>Silva, Jr, Wilson Araújo</creatorcontrib><creatorcontrib>Calmon, Marilia de Freitas</creatorcontrib><creatorcontrib>Rahal, Paula</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valsechi, Marina Curado</au><au>Oliveira, Ana Beatriz Bortolozo</au><au>Conceição, André Luis Giacometti</au><au>Stuqui, Bruna</au><au>Candido, Natalia Maria</au><au>Provazzi, Paola Jocelan Scarin</au><au>de Araújo, Luiza Ferreira</au><au>Silva, Jr, Wilson Araújo</au><au>Calmon, Marilia de Freitas</au><au>Rahal, Paula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GPC3 reduces cell proliferation in renal carcinoma cell lines</atitle><jtitle>BMC cancer</jtitle><addtitle>BMC Cancer</addtitle><date>2014-08-29</date><risdate>2014</risdate><volume>14</volume><issue>1</issue><spage>631</spage><epage>631</epage><pages>631-631</pages><artnum>631</artnum><issn>1471-2407</issn><eissn>1471-2407</eissn><abstract>Glypican 3 (GPC3) is a member of the family of glypican heparan sulfate proteoglycans (HSPGs). The GPC3 gene may play a role in controlling cell migration, negatively regulating cell growth and inducing apoptosis. GPC3 is downregulated in several cancers, which can result in uncontrolled cell growth and can also contribute to the malignant phenotype of some tumors. The purpose of this study was to analyze the mechanism of action of the GPC3 gene in clear cell renal cell carcinoma.
Five clear cell renal cell carcinoma cell lines and carcinoma samples were used to analyze GPC3 mRNA expression (qRT-PCR). Then, representative cell lines, one primary renal carcinoma (786-O) and one metastatic renal carcinoma (ACHN), were chosen to carry out functional studies. We constructed a GPC3 expression vector and transfected the renal carcinoma cell lines, 786-O and ACHN. GPC3 overexpression was analyzed using qRT-PCR and immunocytochemistry. We evaluated cell proliferation using MTT and colony formation assays. Flow cytometry was used to evaluate apoptosis and perform cell cycle analyses.
We observed that GPC3 is downregulated in clear cell renal cell carcinoma samples and cell lines compared with normal renal samples. GPC3 mRNA expression and protein levels in 786-O and ACHN cell lines increased after transfection with the GPC3 expression construct, and the cell proliferation rate decreased in both cell lines following overexpression of GPC3. Further, apoptosis was not induced in the renal cell carcinoma cell lines overexpressing GPC3, and there was an increase in the cell population during the G1 phase in the cell cycle.
We suggest that the GPC3 gene reduces the rate of cell proliferation through cell cycle arrest during the G1 phase in renal cell carcinoma.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>25168166</pmid><doi>10.1186/1471-2407-14-631</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Breast cancer Carcinoma, Renal Cell - genetics Carcinoma, Renal Cell - metabolism Carcinoma, Renal Cell - pathology Cell Cycle Cell growth Cell Line, Tumor Cell Proliferation Deoxyribonucleic acid DNA Extracellular matrix Female Gene expression Gene Expression Regulation, Neoplastic Genomics Glypicans - genetics Glypicans - metabolism Humans Kidney Neoplasms - genetics Kidney Neoplasms - metabolism Kidney Neoplasms - pathology Liver cancer Male Metastasis Middle Aged Polymerase chain reaction Real time Rodents Studies Tumors |
| title | GPC3 reduces cell proliferation in renal carcinoma cell lines |
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