High-Throughput Bead-Based Identification of Structure-Switching Aptamer Beacons

We describe a new platform to identify structure‐switching DNA beacon aptamers, which detect small molecules in a specific manner. By clonally amplifying a DNA library designed to fluoresce in response to binding events onto microbeads, aptamer beacons can be selected by stringent fluorescence‐assis...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2014-09, Vol.15 (13), p.1877-1881
Hauptverfasser:	Trevino, Simon G., Levy, Matthew
Format:	Artikel
Sprache:	eng
Schlagworte:	aptamers Aptamers, Nucleotide - chemistry Binding Sites Communications Computational Biology Deoxyribonucleic acid DNA DNA structures emulsion PCR Fluorescence Gene Library High-Throughput Screening Assays molecular beacons Oligonucleotides - chemistry SELEX SELEX Aptamer Technique Steroids - antagonists & inhibitors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!