NMR-based Structural Analysis of the Complete Rough-type Lipopolysaccharide Isolated from Capnocytophaga canimorsus

We here describe the NMR analysis of an intact lipopolysaccharide (LPS, endotoxin) in water with 1,2-dihexanoyl-sn-glycero-3-phosphocholine as detergent. When HPLC-purified rough-type LPS of Capnocytophaga canimorsus was prepared, 13C,15N labeling could be avoided. The intact LPS was analyzed by hom...

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Veröffentlicht in:	The Journal of biological chemistry 2014-08, Vol.289 (34), p.23963-23976
Hauptverfasser:	Zähringer, Ulrich, Ittig, Simon, Lindner, Buko, Moll, Hermann, Schombel, Ursula, Gisch, Nicolas, Cornelis, Guy R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Capnocytophaga - chemistry Carbohydrate Conformation Carbohydrate Sequence Chromatography, High Pressure Liquid Gas Chromatography-Mass Spectrometry Lipopolysaccharides - chemistry Lipopolysaccharides - isolation & purification Magnetic Resonance Spectroscopy - methods Molecular Bases of Disease Molecular Sequence Data
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