In Vivo Incorporation of Unnatural Amino Acids Using the Chemical Aminoacylation Strategy. A Broadly Applicable Mechanistic Tool

We describe one strategy for incorporating unnatural amino acids site-specifically into proteins expressed in living cells, involving organic synthesis to chemically aminoacylate a suppressor tRNA, protein expression in Xenopus oocytes, and, primarily, monitoring protein function by electrophysiolog...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2014-07, Vol.15 (12), p.1710-1720
Hauptverfasser:	Dougherty, Dennis A., Van Arnam, Ethan B.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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