Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging
Stem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of non-hematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellul...
Gespeichert in:
| Veröffentlicht in: | AGE 2014-08, Vol.36 (4), p.9691-9691, Article 9691 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 9691 |
|---|---|
| container_issue | 4 |
| container_start_page | 9691 |
| container_title | AGE |
| container_volume | 36 |
| creator | Aksoy, Ceren Kaya, Fatima Aerts Kuşkonmaz, Bülent Barış Uçkan, Duygu Severcan, Feride |
| description | Stem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of non-hematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellular microenvironment into BM. Here, we investigated age-related differences in BM-MSCs by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and FTIR imaging together with hierarchical clustering as a novel methods to clarify global alterations in the structure and function of macromolecules in characterized BM-MSCs of different aged donors. The results may contribute to identification of age-related new molecular marker(s) to determine the effects of donor age on MSCs. The spectral results reflected that there were significant increases in the concentration of saturated lipids, proteins, glycogen, and nucleic acids in children and adolescent group BM-MSCs when compared to the infants and early and mid adults. The concentration of mentioned macromolecules in adult (early and mid) BM-MSCs were significantly lower than the concentrations in the children and adolescents. These results were attributed to the increase in the proliferation activity in younger BM-MSCs. The distribution of macromolecules into the cells was shown as in the form of chemical maps by FTIR imaging, and the results are in agreement with the ATR-FTIR spectroscopy results. The cellular activity degree was determined by the thiazolyl blue tetrazolium bromide (MTT) proliferation assay to support ATR-FTIR spectroscopy results. BM-MSCs of five different age groups were discriminated by making the hierarchical cluster analysis where the spectral data according to alterations in structure and composition of macromolecules were considered. |
| doi_str_mv | 10.1007/s11357-014-9691-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4150902</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3419203681</sourcerecordid><originalsourceid>FETCH-LOGICAL-c540t-2a562f4c1e38f79b770e9ea43e67d765b79cf37da27f829eae72cba3625892b13</originalsourceid><addsrcrecordid>eNp1UV1rFTEUDKLYa_UH-CIBX3xZzcnmY9cHoRSrhYKg9Tlksyd7t9xNrsluy_33Zrm1VMGnwDkzk5kzhLwG9h4Y0x8yQC11xUBUrWqh0k_IBqQWlVBCPyUbBgqqFiQ_IS9yvmFMyrrhz8kJl6wBYM2G3P2Y0-LmJdkdHcMt5nkc7DzGQKOnfQwxUTsgRe_RzbSMt8tkA-1iQDrZlOIdnTBjcNvDVCTyjBN1uNvlj_Ti-vI7zfvCSzG7uD9QG3o6TnYYw_CSPPN2l_HV_XtKfl58vj7_Wl19-3J5fnZVOSnYXHErFffCAdaN122nNcMWrahR6V4r2enW-Vr3lmvf8LJBzV1na8Vl0_IO6lPy6ai7X7oJe4dhLlHNPhUf6WCiHc3fmzBuzRBvjQDJWsaLwLt7gRR_LeU-ZhrzmtAGjEs2ICUwzlmjCvTtP9CbuKRQ4q2oRmkm6tURHFGunCUn9A9mgJm1VnOs1ZRazVqr0YXz5nGKB8afHguAHwG5rMKA6dHX_1X9DRImr7E</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1558670431</pqid></control><display><type>article</type><title>Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging</title><source>MEDLINE</source><source>SpringerNature Journals</source><source>PubMed Central</source><creator>Aksoy, Ceren ; Kaya, Fatima Aerts ; Kuşkonmaz, Bülent Barış ; Uçkan, Duygu ; Severcan, Feride</creator><creatorcontrib>Aksoy, Ceren ; Kaya, Fatima Aerts ; Kuşkonmaz, Bülent Barış ; Uçkan, Duygu ; Severcan, Feride</creatorcontrib><description>Stem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of non-hematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellular microenvironment into BM. Here, we investigated age-related differences in BM-MSCs by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and FTIR imaging together with hierarchical clustering as a novel methods to clarify global alterations in the structure and function of macromolecules in characterized BM-MSCs of different aged donors. The results may contribute to identification of age-related new molecular marker(s) to determine the effects of donor age on MSCs. The spectral results reflected that there were significant increases in the concentration of saturated lipids, proteins, glycogen, and nucleic acids in children and adolescent group BM-MSCs when compared to the infants and early and mid adults. The concentration of mentioned macromolecules in adult (early and mid) BM-MSCs were significantly lower than the concentrations in the children and adolescents. These results were attributed to the increase in the proliferation activity in younger BM-MSCs. The distribution of macromolecules into the cells was shown as in the form of chemical maps by FTIR imaging, and the results are in agreement with the ATR-FTIR spectroscopy results. The cellular activity degree was determined by the thiazolyl blue tetrazolium bromide (MTT) proliferation assay to support ATR-FTIR spectroscopy results. BM-MSCs of five different age groups were discriminated by making the hierarchical cluster analysis where the spectral data according to alterations in structure and composition of macromolecules were considered.</description><identifier>ISSN: 0161-9152</identifier><identifier>ISSN: 2509-2715</identifier><identifier>EISSN: 1574-4647</identifier><identifier>EISSN: 2509-2723</identifier><identifier>DOI: 10.1007/s11357-014-9691-7</identifier><identifier>PMID: 25081108</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Adipocytes ; Adolescent ; Adult ; Age ; Age groups ; Aging ; Aging - metabolism ; Analysis ; Biomedical and Life Sciences ; Bone marrow ; Bone Marrow Cells - chemistry ; Bone Marrow Cells - cytology ; Cell Biology ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Child ; Child, Preschool ; Cluster analysis ; Female ; Fourier transforms ; Geriatrics/Gerontology ; Hematology ; Hematopoietic Stem Cells - chemistry ; Hematopoietic Stem Cells - cytology ; Homeostasis ; Humans ; Infant ; Infant, Newborn ; Investigations ; Life Sciences ; Male ; Medicine ; Mesenchymal Stromal Cells - chemistry ; Mesenchymal Stromal Cells - cytology ; Middle Aged ; Molecular Medicine ; Multiprotein Complexes - analysis ; Older people ; Regenerative medicine ; Repair & maintenance ; Spectroscopy, Fourier Transform Infrared - methods ; Spectrum analysis ; Stem cells ; Studies ; Tissue engineering ; Tissues ; Young Adult</subject><ispartof>AGE, 2014-08, Vol.36 (4), p.9691-9691, Article 9691</ispartof><rights>American Aging Association 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-2a562f4c1e38f79b770e9ea43e67d765b79cf37da27f829eae72cba3625892b13</citedby><cites>FETCH-LOGICAL-c540t-2a562f4c1e38f79b770e9ea43e67d765b79cf37da27f829eae72cba3625892b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150902/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4150902/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25081108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aksoy, Ceren</creatorcontrib><creatorcontrib>Kaya, Fatima Aerts</creatorcontrib><creatorcontrib>Kuşkonmaz, Bülent Barış</creatorcontrib><creatorcontrib>Uçkan, Duygu</creatorcontrib><creatorcontrib>Severcan, Feride</creatorcontrib><title>Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging</title><title>AGE</title><addtitle>AGE</addtitle><addtitle>Age (Dordr)</addtitle><description>Stem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of non-hematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellular microenvironment into BM. Here, we investigated age-related differences in BM-MSCs by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and FTIR imaging together with hierarchical clustering as a novel methods to clarify global alterations in the structure and function of macromolecules in characterized BM-MSCs of different aged donors. The results may contribute to identification of age-related new molecular marker(s) to determine the effects of donor age on MSCs. The spectral results reflected that there were significant increases in the concentration of saturated lipids, proteins, glycogen, and nucleic acids in children and adolescent group BM-MSCs when compared to the infants and early and mid adults. The concentration of mentioned macromolecules in adult (early and mid) BM-MSCs were significantly lower than the concentrations in the children and adolescents. These results were attributed to the increase in the proliferation activity in younger BM-MSCs. The distribution of macromolecules into the cells was shown as in the form of chemical maps by FTIR imaging, and the results are in agreement with the ATR-FTIR spectroscopy results. The cellular activity degree was determined by the thiazolyl blue tetrazolium bromide (MTT) proliferation assay to support ATR-FTIR spectroscopy results. BM-MSCs of five different age groups were discriminated by making the hierarchical cluster analysis where the spectral data according to alterations in structure and composition of macromolecules were considered.</description><subject>Adipocytes</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Age</subject><subject>Age groups</subject><subject>Aging</subject><subject>Aging - metabolism</subject><subject>Analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - chemistry</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cell Biology</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cluster analysis</subject><subject>Female</subject><subject>Fourier transforms</subject><subject>Geriatrics/Gerontology</subject><subject>Hematology</subject><subject>Hematopoietic Stem Cells - chemistry</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Investigations</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medicine</subject><subject>Mesenchymal Stromal Cells - chemistry</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Middle Aged</subject><subject>Molecular Medicine</subject><subject>Multiprotein Complexes - analysis</subject><subject>Older people</subject><subject>Regenerative medicine</subject><subject>Repair & maintenance</subject><subject>Spectroscopy, Fourier Transform Infrared - methods</subject><subject>Spectrum analysis</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Tissue engineering</subject><subject>Tissues</subject><subject>Young Adult</subject><issn>0161-9152</issn><issn>2509-2715</issn><issn>1574-4647</issn><issn>2509-2723</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UV1rFTEUDKLYa_UH-CIBX3xZzcnmY9cHoRSrhYKg9Tlksyd7t9xNrsluy_33Zrm1VMGnwDkzk5kzhLwG9h4Y0x8yQC11xUBUrWqh0k_IBqQWlVBCPyUbBgqqFiQ_IS9yvmFMyrrhz8kJl6wBYM2G3P2Y0-LmJdkdHcMt5nkc7DzGQKOnfQwxUTsgRe_RzbSMt8tkA-1iQDrZlOIdnTBjcNvDVCTyjBN1uNvlj_Ti-vI7zfvCSzG7uD9QG3o6TnYYw_CSPPN2l_HV_XtKfl58vj7_Wl19-3J5fnZVOSnYXHErFffCAdaN122nNcMWrahR6V4r2enW-Vr3lmvf8LJBzV1na8Vl0_IO6lPy6ai7X7oJe4dhLlHNPhUf6WCiHc3fmzBuzRBvjQDJWsaLwLt7gRR_LeU-ZhrzmtAGjEs2ICUwzlmjCvTtP9CbuKRQ4q2oRmkm6tURHFGunCUn9A9mgJm1VnOs1ZRazVqr0YXz5nGKB8afHguAHwG5rMKA6dHX_1X9DRImr7E</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Aksoy, Ceren</creator><creator>Kaya, Fatima Aerts</creator><creator>Kuşkonmaz, Bülent Barış</creator><creator>Uçkan, Duygu</creator><creator>Severcan, Feride</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7QG</scope><scope>7RV</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88J</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HEHIP</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>KB0</scope><scope>L.-</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2R</scope><scope>M2S</scope><scope>NAPCQ</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYYUZ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140801</creationdate><title>Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging</title><author>Aksoy, Ceren ; Kaya, Fatima Aerts ; Kuşkonmaz, Bülent Barış ; Uçkan, Duygu ; Severcan, Feride</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-2a562f4c1e38f79b770e9ea43e67d765b79cf37da27f829eae72cba3625892b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adipocytes</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Age</topic><topic>Age groups</topic><topic>Aging</topic><topic>Aging - metabolism</topic><topic>Analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - chemistry</topic><topic>Bone Marrow Cells - cytology</topic><topic>Cell Biology</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cluster analysis</topic><topic>Female</topic><topic>Fourier transforms</topic><topic>Geriatrics/Gerontology</topic><topic>Hematology</topic><topic>Hematopoietic Stem Cells - chemistry</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Investigations</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medicine</topic><topic>Mesenchymal Stromal Cells - chemistry</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Middle Aged</topic><topic>Molecular Medicine</topic><topic>Multiprotein Complexes - analysis</topic><topic>Older people</topic><topic>Regenerative medicine</topic><topic>Repair & maintenance</topic><topic>Spectroscopy, Fourier Transform Infrared - methods</topic><topic>Spectrum analysis</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Tissue engineering</topic><topic>Tissues</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aksoy, Ceren</creatorcontrib><creatorcontrib>Kaya, Fatima Aerts</creatorcontrib><creatorcontrib>Kuşkonmaz, Bülent Barış</creatorcontrib><creatorcontrib>Uçkan, Duygu</creatorcontrib><creatorcontrib>Severcan, Feride</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Access via ABI/INFORM (ProQuest)</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Social Science Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Social Science Premium Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Sociology Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Social Science Database</collection><collection>Sociology Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ABI/INFORM Collection China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>AGE</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aksoy, Ceren</au><au>Kaya, Fatima Aerts</au><au>Kuşkonmaz, Bülent Barış</au><au>Uçkan, Duygu</au><au>Severcan, Feride</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging</atitle><jtitle>AGE</jtitle><stitle>AGE</stitle><addtitle>Age (Dordr)</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>36</volume><issue>4</issue><spage>9691</spage><epage>9691</epage><pages>9691-9691</pages><artnum>9691</artnum><issn>0161-9152</issn><issn>2509-2715</issn><eissn>1574-4647</eissn><eissn>2509-2723</eissn><abstract>Stem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of non-hematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellular microenvironment into BM. Here, we investigated age-related differences in BM-MSCs by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and FTIR imaging together with hierarchical clustering as a novel methods to clarify global alterations in the structure and function of macromolecules in characterized BM-MSCs of different aged donors. The results may contribute to identification of age-related new molecular marker(s) to determine the effects of donor age on MSCs. The spectral results reflected that there were significant increases in the concentration of saturated lipids, proteins, glycogen, and nucleic acids in children and adolescent group BM-MSCs when compared to the infants and early and mid adults. The concentration of mentioned macromolecules in adult (early and mid) BM-MSCs were significantly lower than the concentrations in the children and adolescents. These results were attributed to the increase in the proliferation activity in younger BM-MSCs. The distribution of macromolecules into the cells was shown as in the form of chemical maps by FTIR imaging, and the results are in agreement with the ATR-FTIR spectroscopy results. The cellular activity degree was determined by the thiazolyl blue tetrazolium bromide (MTT) proliferation assay to support ATR-FTIR spectroscopy results. BM-MSCs of five different age groups were discriminated by making the hierarchical cluster analysis where the spectral data according to alterations in structure and composition of macromolecules were considered.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>25081108</pmid><doi>10.1007/s11357-014-9691-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0161-9152 |
| ispartof | AGE, 2014-08, Vol.36 (4), p.9691-9691, Article 9691 |
| issn | 0161-9152 2509-2715 1574-4647 2509-2723 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4150902 |
| source | MEDLINE; SpringerNature Journals; PubMed Central |
| subjects | Adipocytes Adolescent Adult Age Age groups Aging Aging - metabolism Analysis Biomedical and Life Sciences Bone marrow Bone Marrow Cells - chemistry Bone Marrow Cells - cytology Cell Biology Cell Differentiation Cell Proliferation Cells, Cultured Child Child, Preschool Cluster analysis Female Fourier transforms Geriatrics/Gerontology Hematology Hematopoietic Stem Cells - chemistry Hematopoietic Stem Cells - cytology Homeostasis Humans Infant Infant, Newborn Investigations Life Sciences Male Medicine Mesenchymal Stromal Cells - chemistry Mesenchymal Stromal Cells - cytology Middle Aged Molecular Medicine Multiprotein Complexes - analysis Older people Regenerative medicine Repair & maintenance Spectroscopy, Fourier Transform Infrared - methods Spectrum analysis Stem cells Studies Tissue engineering Tissues Young Adult |
| title | Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T16%3A57%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Structural%20investigation%20of%20donor%20age%20effect%20on%20human%20bone%20marrow%20mesenchymal%20stem%20cells:%20FTIR%20spectroscopy%20and%20imaging&rft.jtitle=AGE&rft.au=Aksoy,%20Ceren&rft.date=2014-08-01&rft.volume=36&rft.issue=4&rft.spage=9691&rft.epage=9691&rft.pages=9691-9691&rft.artnum=9691&rft.issn=0161-9152&rft.eissn=1574-4647&rft_id=info:doi/10.1007/s11357-014-9691-7&rft_dat=%3Cproquest_pubme%3E3419203681%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1558670431&rft_id=info:pmid/25081108&rfr_iscdi=true |