A simple in vitro method for evaluating dendritic cell-based vaccinations
Dendritic cell (DC) therapy is a promising therapy for cancer-targeting treatments. Recently, DCs have been used for treatment of some cancers. We aimed to develop an in vitro assay to evaluate DC therapy in cancer treatment using a breast cancer model. DCs were induced from murine bone marrow monon...
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| Veröffentlicht in: | OncoTargets and therapy 2014-01, Vol.7, p.1455-1464 |
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| container_title | OncoTargets and therapy |
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| creator | Pham, Phuc Van Nguyen, Nhung Thi Nguyen, Hoang Minh Khuat, Lam Tan Le, Phong Minh Pham, Viet Quoc Nguyen, Sinh Truong Phan, Ngoc Kim |
| description | Dendritic cell (DC) therapy is a promising therapy for cancer-targeting treatments. Recently, DCs have been used for treatment of some cancers. We aimed to develop an in vitro assay to evaluate DC therapy in cancer treatment using a breast cancer model.
DCs were induced from murine bone marrow mononuclear cells in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with GM-CSF (20 ng/mL) and IL-4 (20 ng/mL). Immature DCs were primed with breast cancer stem cell (BCSC)-derived antigens. BCSCs were sorted from 4T1 cell lines based on aldehyde dehydrogenase expression. A mixture of DCs and cytotoxic T lymphocytes (CTLs) were used to evaluate the inhibitory effect of antigen-primed DCs on BCSCs. BCSC proliferation and doubling time were recorded based on impedance-based cell analysis using the xCELLigence system. The specification of inhibitory effects of DCs and CTLs was also evaluated using the same system.
The results showed that impedance-based analysis of BCSCs reflected cytotoxicity and inhibitory effects of DCs and CTLs at 72 hours. Differences in ratios of DC:CTL changed the cytotoxicity of DCs and CTLs.
This study successfully used impedance-based cell analysis as a new in vitro assay to evaluate DC efficacy in cancer immunotherapy. We hope this technique will contribute to the development and improvement of immunotherapies in the near future. |
| doi_str_mv | 10.2147/OTT.S67057 |
| format | Article |
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DCs were induced from murine bone marrow mononuclear cells in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with GM-CSF (20 ng/mL) and IL-4 (20 ng/mL). Immature DCs were primed with breast cancer stem cell (BCSC)-derived antigens. BCSCs were sorted from 4T1 cell lines based on aldehyde dehydrogenase expression. A mixture of DCs and cytotoxic T lymphocytes (CTLs) were used to evaluate the inhibitory effect of antigen-primed DCs on BCSCs. BCSC proliferation and doubling time were recorded based on impedance-based cell analysis using the xCELLigence system. The specification of inhibitory effects of DCs and CTLs was also evaluated using the same system.
The results showed that impedance-based analysis of BCSCs reflected cytotoxicity and inhibitory effects of DCs and CTLs at 72 hours. Differences in ratios of DC:CTL changed the cytotoxicity of DCs and CTLs.
This study successfully used impedance-based cell analysis as a new in vitro assay to evaluate DC efficacy in cancer immunotherapy. We hope this technique will contribute to the development and improvement of immunotherapies in the near future.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S67057</identifier><identifier>PMID: 25170272</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Biological products ; Cancer ; Care and treatment ; CTLs ; cytotoxicity assay ; Dendritic cells ; Health aspects ; immunotherapy ; Methodology ; targeting therapy</subject><ispartof>OncoTargets and therapy, 2014-01, Vol.7, p.1455-1464</ispartof><rights>COPYRIGHT 2014 Dove Medical Press Limited</rights><rights>2014 Pham et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-8742eae0118b67ad3b283ab22c5fdfa4ecfd67e3bf45938859b374928323c4c83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145728/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145728/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3849,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25170272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pham, Phuc Van</creatorcontrib><creatorcontrib>Nguyen, Nhung Thi</creatorcontrib><creatorcontrib>Nguyen, Hoang Minh</creatorcontrib><creatorcontrib>Khuat, Lam Tan</creatorcontrib><creatorcontrib>Le, Phong Minh</creatorcontrib><creatorcontrib>Pham, Viet Quoc</creatorcontrib><creatorcontrib>Nguyen, Sinh Truong</creatorcontrib><creatorcontrib>Phan, Ngoc Kim</creatorcontrib><title>A simple in vitro method for evaluating dendritic cell-based vaccinations</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>Dendritic cell (DC) therapy is a promising therapy for cancer-targeting treatments. Recently, DCs have been used for treatment of some cancers. We aimed to develop an in vitro assay to evaluate DC therapy in cancer treatment using a breast cancer model.
DCs were induced from murine bone marrow mononuclear cells in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with GM-CSF (20 ng/mL) and IL-4 (20 ng/mL). Immature DCs were primed with breast cancer stem cell (BCSC)-derived antigens. BCSCs were sorted from 4T1 cell lines based on aldehyde dehydrogenase expression. A mixture of DCs and cytotoxic T lymphocytes (CTLs) were used to evaluate the inhibitory effect of antigen-primed DCs on BCSCs. BCSC proliferation and doubling time were recorded based on impedance-based cell analysis using the xCELLigence system. The specification of inhibitory effects of DCs and CTLs was also evaluated using the same system.
The results showed that impedance-based analysis of BCSCs reflected cytotoxicity and inhibitory effects of DCs and CTLs at 72 hours. Differences in ratios of DC:CTL changed the cytotoxicity of DCs and CTLs.
This study successfully used impedance-based cell analysis as a new in vitro assay to evaluate DC efficacy in cancer immunotherapy. We hope this technique will contribute to the development and improvement of immunotherapies in the near future.</description><subject>Biological products</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>CTLs</subject><subject>cytotoxicity assay</subject><subject>Dendritic cells</subject><subject>Health aspects</subject><subject>immunotherapy</subject><subject>Methodology</subject><subject>targeting therapy</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNptkl1rFDEUhgdRbK3e-ANkQBARZpuvmWRuhKWoLRR60fU6ZJIzu5FMsiYzA_57s53a7oLkIuHkec8Xb1G8x2hFMOOXd5vN6r7hqOYvinOMuaialqKXR--z4k1KvxBqGkHY6-KM1Jgjwsl5cbMukx32Dkrry9mOMZQDjLtgyj7EEmblJjVavy0NeBPtaHWpwbmqUwlMOSutrc9A8Olt8apXLsG7x_ui-Pn92-bqurq9-3Fztb6tdC46VoIzAgoQxqJruDK0I4KqjhBd96ZXDHRvGg6061ndUiHqtqOctRkiVDMt6EXxdcm7n7oBjAY_RuXkPtpBxT8yKCtPf7zdyW2YJcOs5uSQAC0JTJhhHyGlE_FzVIdBYt4KlCWfH2vG8HuCNMrBpsMelIcwJYnrWtSkFYRm9OOCbpUDaX0fchP6gMs1w7jBVDz0sPoPlY-Bwergobc5fiL4dCTYgXLjLgU3Paz-FPyygDqGlCL0T8NhJA92kdkucrFLhj8c7_IJ_ecP-hfGurqL</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Pham, Phuc Van</creator><creator>Nguyen, Nhung Thi</creator><creator>Nguyen, Hoang Minh</creator><creator>Khuat, Lam Tan</creator><creator>Le, Phong Minh</creator><creator>Pham, Viet Quoc</creator><creator>Nguyen, Sinh Truong</creator><creator>Phan, Ngoc Kim</creator><general>Dove Medical Press Limited</general><general>Dove Press</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140101</creationdate><title>A simple in vitro method for evaluating dendritic cell-based vaccinations</title><author>Pham, Phuc Van ; Nguyen, Nhung Thi ; Nguyen, Hoang Minh ; Khuat, Lam Tan ; Le, Phong Minh ; Pham, Viet Quoc ; Nguyen, Sinh Truong ; Phan, Ngoc Kim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-8742eae0118b67ad3b283ab22c5fdfa4ecfd67e3bf45938859b374928323c4c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological products</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>CTLs</topic><topic>cytotoxicity assay</topic><topic>Dendritic cells</topic><topic>Health aspects</topic><topic>immunotherapy</topic><topic>Methodology</topic><topic>targeting therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pham, Phuc Van</creatorcontrib><creatorcontrib>Nguyen, Nhung Thi</creatorcontrib><creatorcontrib>Nguyen, Hoang Minh</creatorcontrib><creatorcontrib>Khuat, Lam Tan</creatorcontrib><creatorcontrib>Le, Phong Minh</creatorcontrib><creatorcontrib>Pham, Viet Quoc</creatorcontrib><creatorcontrib>Nguyen, Sinh Truong</creatorcontrib><creatorcontrib>Phan, Ngoc Kim</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pham, Phuc Van</au><au>Nguyen, Nhung Thi</au><au>Nguyen, Hoang Minh</au><au>Khuat, Lam Tan</au><au>Le, Phong Minh</au><au>Pham, Viet Quoc</au><au>Nguyen, Sinh Truong</au><au>Phan, Ngoc Kim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple in vitro method for evaluating dendritic cell-based vaccinations</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>7</volume><spage>1455</spage><epage>1464</epage><pages>1455-1464</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Dendritic cell (DC) therapy is a promising therapy for cancer-targeting treatments. Recently, DCs have been used for treatment of some cancers. We aimed to develop an in vitro assay to evaluate DC therapy in cancer treatment using a breast cancer model.
DCs were induced from murine bone marrow mononuclear cells in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with GM-CSF (20 ng/mL) and IL-4 (20 ng/mL). Immature DCs were primed with breast cancer stem cell (BCSC)-derived antigens. BCSCs were sorted from 4T1 cell lines based on aldehyde dehydrogenase expression. A mixture of DCs and cytotoxic T lymphocytes (CTLs) were used to evaluate the inhibitory effect of antigen-primed DCs on BCSCs. BCSC proliferation and doubling time were recorded based on impedance-based cell analysis using the xCELLigence system. The specification of inhibitory effects of DCs and CTLs was also evaluated using the same system.
The results showed that impedance-based analysis of BCSCs reflected cytotoxicity and inhibitory effects of DCs and CTLs at 72 hours. Differences in ratios of DC:CTL changed the cytotoxicity of DCs and CTLs.
This study successfully used impedance-based cell analysis as a new in vitro assay to evaluate DC efficacy in cancer immunotherapy. We hope this technique will contribute to the development and improvement of immunotherapies in the near future.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>25170272</pmid><doi>10.2147/OTT.S67057</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Biological products Cancer Care and treatment CTLs cytotoxicity assay Dendritic cells Health aspects immunotherapy Methodology targeting therapy |
| title | A simple in vitro method for evaluating dendritic cell-based vaccinations |
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