Functional cooperation of mitochondrial protein import receptors in yeast
We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor. We cloned, sequenced and physically mapped the MAS20 gene and found that the protein is homologous to the MOM19 import receptor from Neurospora crassa. MAS20...
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| Veröffentlicht in: | The EMBO journal 1993-11, Vol.12 (11), p.4115-4123 |
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| description | We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor. We cloned, sequenced and physically mapped the MAS20 gene and found that the protein is homologous to the MOM19 import receptor from Neurospora crassa. MAS20 and MOM19 contain the sequence motif F-X-K-A-L-X-V/L, which is repeated several times with minor variations in the MAS70/MOM72 receptors. To determine how MAS20 functions together with the previously identified yeast receptor MAS70, we constructed yeast mutants lacking either one or both of the receptors. Deletion of either receptor alone had little or no effect on fermentative growth and only partially inhibited mitochondrial protein import in vivo. Deletion of both receptors was lethal. Deleting only MAS70 did not affect respiration; deleting only MAS20 caused loss of respiration, but respiration could be restored by overexpressing MAS70. Import of the F1-ATPase beta-subunit into isolated mitochondria was only partly inhibited by IgGs against either MAS20 or MAS70, but both IgGs inhibited import completely. We conclude that the two receptors have overlapping specificities for mitochondrial precursor proteins and that neither receptor is by itself essential. |
| doi_str_mv | 10.1002/j.1460-2075.1993.tb06095.x |
| format | Article |
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We cloned, sequenced and physically mapped the MAS20 gene and found that the protein is homologous to the MOM19 import receptor from Neurospora crassa. MAS20 and MOM19 contain the sequence motif F-X-K-A-L-X-V/L, which is repeated several times with minor variations in the MAS70/MOM72 receptors. To determine how MAS20 functions together with the previously identified yeast receptor MAS70, we constructed yeast mutants lacking either one or both of the receptors. Deletion of either receptor alone had little or no effect on fermentative growth and only partially inhibited mitochondrial protein import in vivo. Deletion of both receptors was lethal. Deleting only MAS70 did not affect respiration; deleting only MAS20 caused loss of respiration, but respiration could be restored by overexpressing MAS70. Import of the F1-ATPase beta-subunit into isolated mitochondria was only partly inhibited by IgGs against either MAS20 or MAS70, but both IgGs inhibited import completely. We conclude that the two receptors have overlapping specificities for mitochondrial precursor proteins and that neither receptor is by itself essential.</description><identifier>ISSN: 0261-4189</identifier><identifier>EISSN: 1460-2075</identifier><identifier>DOI: 10.1002/j.1460-2075.1993.tb06095.x</identifier><identifier>PMID: 8223428</identifier><identifier>CODEN: EMJODG</identifier><language>eng</language><publisher>London: Nature Publishing Group</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Base Sequence ; Biological and medical sciences ; Biological Transport ; Cell Compartmentation ; cell membranes ; Chromosome Mapping ; Chromosomes, Fungal ; deletions ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - metabolism ; Gene Deletion ; Genes, Lethal ; Genomic Library ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; mas20 gene ; mas20 protein ; Membrane Proteins - genetics ; Membrane Proteins - isolation & purification ; Membrane Proteins - metabolism ; Microbiology ; mitochondria ; Mitochondria - metabolism ; Mitochondrial Membrane Transport Proteins ; mitochondrial outer membranes ; Mitochondrial Precursor Protein Import Complex Proteins ; Models, Biological ; Molecular Sequence Data ; mutagenesis ; mutants ; Mycology ; nucleotide sequences ; Protein Precursors - metabolism ; protein transport ; proteins ; Proton-Translocating ATPases - metabolism ; receptors ; Receptors, Cytoplasmic and Nuclear - genetics ; Receptors, Cytoplasmic and Nuclear - isolation & purification ; Receptors, Cytoplasmic and Nuclear - metabolism ; Recombinant Proteins - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Sequence Homology, Amino Acid ; structural genes</subject><ispartof>The EMBO journal, 1993-11, Vol.12 (11), p.4115-4123</ispartof><rights>1993 European Molecular Biology Organization</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6285-e315ce8d47c21cbe02dd757506474be8d0744012b2939b1c5532cc1ea06c68443</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC413704/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC413704/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3757773$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8223428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramage, L</creatorcontrib><creatorcontrib>Junne, T</creatorcontrib><creatorcontrib>Hahne, K</creatorcontrib><creatorcontrib>Lithgow, T</creatorcontrib><creatorcontrib>Schatz, G</creatorcontrib><title>Functional cooperation of mitochondrial protein import receptors in yeast</title><title>The EMBO journal</title><addtitle>EMBO J</addtitle><description>We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor. We cloned, sequenced and physically mapped the MAS20 gene and found that the protein is homologous to the MOM19 import receptor from Neurospora crassa. MAS20 and MOM19 contain the sequence motif F-X-K-A-L-X-V/L, which is repeated several times with minor variations in the MAS70/MOM72 receptors. To determine how MAS20 functions together with the previously identified yeast receptor MAS70, we constructed yeast mutants lacking either one or both of the receptors. Deletion of either receptor alone had little or no effect on fermentative growth and only partially inhibited mitochondrial protein import in vivo. Deletion of both receptors was lethal. Deleting only MAS70 did not affect respiration; deleting only MAS20 caused loss of respiration, but respiration could be restored by overexpressing MAS70. Import of the F1-ATPase beta-subunit into isolated mitochondria was only partly inhibited by IgGs against either MAS20 or MAS70, but both IgGs inhibited import completely. We conclude that the two receptors have overlapping specificities for mitochondrial precursor proteins and that neither receptor is by itself essential.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Cell Compartmentation</subject><subject>cell membranes</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Fungal</subject><subject>deletions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - metabolism</subject><subject>Gene Deletion</subject><subject>Genes, Lethal</subject><subject>Genomic Library</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>mas20 gene</subject><subject>mas20 protein</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Membrane Proteins - metabolism</subject><subject>Microbiology</subject><subject>mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>Mitochondrial Membrane Transport Proteins</subject><subject>mitochondrial outer membranes</subject><subject>Mitochondrial Precursor Protein Import Complex Proteins</subject><subject>Models, Biological</subject><subject>Molecular Sequence Data</subject><subject>mutagenesis</subject><subject>mutants</subject><subject>Mycology</subject><subject>nucleotide sequences</subject><subject>Protein Precursors - metabolism</subject><subject>protein transport</subject><subject>proteins</subject><subject>Proton-Translocating ATPases - metabolism</subject><subject>receptors</subject><subject>Receptors, Cytoplasmic and Nuclear - genetics</subject><subject>Receptors, Cytoplasmic and Nuclear - isolation & purification</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>Recombinant Proteins - metabolism</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>structural genes</subject><issn>0261-4189</issn><issn>1460-2075</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUctu1DAUtRCoDIVPQEQIsUu4fsUOEotStVBUxAK6thzHaT1K4tT2QOfvcZhoBEtWfpzHvToHodcYKgxA3m0rzGooCQhe4aahVWqhhoZXD4_Q5gg9RhsgNS4Zls1T9CzGLQBwKfAJOpGEUEbkBl1d7iaTnJ_0UBjvZxv08ip8X4wueXPnpy64DM7BJ-umwo2zD6kI1tg5-RCL_Le3Oqbn6Emvh2hfrOcpurm8-HH-ubz-9unq_Oy6NDWRvLQUc2Nlx4Qh2LQWSNcJLjjUTLA2AyAYA0xa0tCmxYZzSozBVkNtaskYPUUfDr7zrh1tZ-yUgh7UHNyow1557dS_yOTu1K3_qRimAhb921Uf_P3OxqRGF40dBj1Zv4sK10IApzIT3x-IJvgYg-2PMzCopQe1VUvYaglbLT2otQf1kMUv_97yKF2Dz_ibFdfR6KEPejIuHmk0RyIEzbSzA-2XG-z-PxZQF18_fvlzzx6vDh699krfhjzm5jsBTAEzSQlQ-ht_jbBL</recordid><startdate>199311</startdate><enddate>199311</enddate><creator>Ramage, L</creator><creator>Junne, T</creator><creator>Hahne, K</creator><creator>Lithgow, T</creator><creator>Schatz, G</creator><general>Nature Publishing Group</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>199311</creationdate><title>Functional cooperation of mitochondrial protein import receptors in yeast</title><author>Ramage, L ; Junne, T ; Hahne, K ; Lithgow, T ; Schatz, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6285-e315ce8d47c21cbe02dd757506474be8d0744012b2939b1c5532cc1ea06c68443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Cell Compartmentation</topic><topic>cell membranes</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Fungal</topic><topic>deletions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal Proteins - metabolism</topic><topic>Gene Deletion</topic><topic>Genes, Lethal</topic><topic>Genomic Library</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>mas20 gene</topic><topic>mas20 protein</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Membrane Proteins - metabolism</topic><topic>Microbiology</topic><topic>mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>Mitochondrial Membrane Transport Proteins</topic><topic>mitochondrial outer membranes</topic><topic>Mitochondrial Precursor Protein Import Complex Proteins</topic><topic>Models, Biological</topic><topic>Molecular Sequence Data</topic><topic>mutagenesis</topic><topic>mutants</topic><topic>Mycology</topic><topic>nucleotide sequences</topic><topic>Protein Precursors - metabolism</topic><topic>protein transport</topic><topic>proteins</topic><topic>Proton-Translocating ATPases - metabolism</topic><topic>receptors</topic><topic>Receptors, Cytoplasmic and Nuclear - genetics</topic><topic>Receptors, Cytoplasmic and Nuclear - isolation & purification</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>Recombinant Proteins - metabolism</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>structural genes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramage, L</creatorcontrib><creatorcontrib>Junne, T</creatorcontrib><creatorcontrib>Hahne, K</creatorcontrib><creatorcontrib>Lithgow, T</creatorcontrib><creatorcontrib>Schatz, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The EMBO journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramage, L</au><au>Junne, T</au><au>Hahne, K</au><au>Lithgow, T</au><au>Schatz, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional cooperation of mitochondrial protein import receptors in yeast</atitle><jtitle>The EMBO journal</jtitle><addtitle>EMBO J</addtitle><date>1993-11</date><risdate>1993</risdate><volume>12</volume><issue>11</issue><spage>4115</spage><epage>4123</epage><pages>4115-4123</pages><issn>0261-4189</issn><eissn>1460-2075</eissn><coden>EMJODG</coden><abstract>We have identified a 20 kDa yeast mitochondrial outer membrane protein (termed MAS20) which appears to function as a protein import receptor. We cloned, sequenced and physically mapped the MAS20 gene and found that the protein is homologous to the MOM19 import receptor from Neurospora crassa. MAS20 and MOM19 contain the sequence motif F-X-K-A-L-X-V/L, which is repeated several times with minor variations in the MAS70/MOM72 receptors. To determine how MAS20 functions together with the previously identified yeast receptor MAS70, we constructed yeast mutants lacking either one or both of the receptors. Deletion of either receptor alone had little or no effect on fermentative growth and only partially inhibited mitochondrial protein import in vivo. Deletion of both receptors was lethal. Deleting only MAS70 did not affect respiration; deleting only MAS20 caused loss of respiration, but respiration could be restored by overexpressing MAS70. Import of the F1-ATPase beta-subunit into isolated mitochondria was only partly inhibited by IgGs against either MAS20 or MAS70, but both IgGs inhibited import completely. We conclude that the two receptors have overlapping specificities for mitochondrial precursor proteins and that neither receptor is by itself essential.</abstract><cop>London</cop><pub>Nature Publishing Group</pub><pmid>8223428</pmid><doi>10.1002/j.1460-2075.1993.tb06095.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences Biological Transport Cell Compartmentation cell membranes Chromosome Mapping Chromosomes, Fungal deletions Fundamental and applied biological sciences. Psychology Fungal Proteins - metabolism Gene Deletion Genes, Lethal Genomic Library Growth, nutrition, metabolism, transports, enzymes. Molecular biology mas20 gene mas20 protein Membrane Proteins - genetics Membrane Proteins - isolation & purification Membrane Proteins - metabolism Microbiology mitochondria Mitochondria - metabolism Mitochondrial Membrane Transport Proteins mitochondrial outer membranes Mitochondrial Precursor Protein Import Complex Proteins Models, Biological Molecular Sequence Data mutagenesis mutants Mycology nucleotide sequences Protein Precursors - metabolism protein transport proteins Proton-Translocating ATPases - metabolism receptors Receptors, Cytoplasmic and Nuclear - genetics Receptors, Cytoplasmic and Nuclear - isolation & purification Receptors, Cytoplasmic and Nuclear - metabolism Recombinant Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid structural genes |
| title | Functional cooperation of mitochondrial protein import receptors in yeast |
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